scholarly journals Mouse Embryonic Development in a Serum-free Whole Embryo Culture System

10.3791/50803 ◽  
2014 ◽  
Author(s):  
Vijay K. Kalaskar ◽  
James D. Lauderdale
Keyword(s):  
Embryonic Development ◽  
Embryo Culture ◽  
Culture System ◽  
Whole Embryo Culture ◽  
Serum Free

The method of chicken whole embryo culture using the eggshell windowing, surrogate eggshell and ex ovo culture system

2018 ◽  
Vol 59 (2) ◽  
pp. 240-244 ◽  
Author(s):  
M. Farzaneh ◽  
F. Attari ◽  
S. E. Khoshnam ◽  
P. E. Mozdziak
Keyword(s):  
Embryo Culture ◽  
Culture System ◽  
Whole Embryo Culture

scholarly journals Exogenous Nucleotides Antagonize the Developmental Toxicity of EthanolIn Vitro

2013 ◽  
Vol 2013 ◽  
pp. 1-8
Author(s):  
Jie Zhao ◽  
Jia-Xi Zhao ◽  
Ya-Jun Xu
Keyword(s):  
Birth Defects ◽  
Embryo Culture ◽  
Culture System ◽  
Growth Parameters ◽  
Developmental Toxicity ◽  
Micromass Culture ◽  
Protective Effects ◽  
Whole Embryo Culture ◽  
Proliferation And Differentiation

The objective of this study was to assess whether nucleotides supplementationin vitrocould suppress ethanol-induced developmental toxicity in mouse. The models of whole embryo culture (WEC) and midbrain (MB) cell micromass culture were used in this study. In WEC system, exposure to 4.0 mg/mL ethanol for 48 h yielded various developmental malformations of the mice embryos. Nucleotides supplementation (0.16, 0.80, 4.00, 20.00, and 100.00 mg/L) improved the growth parameters to some extent, and the protective effects peaked at 4.00 mg/L. In MB cell micromass culture system, exposure to 4.0 mg/mL ethanol for 5 days resulted in suppression of proliferation and differentiation. Supplementation of nucleotides (0.16, 0.80, 4.00, 20.00, and 100.00 mg/L) showed some protective effects, which peaked at 4.00 mg/L, too. The present research indicated that nucleotides supplementation might be of some benefit in the prevention of ethanol-induced birth defects; however, appropriate dosage requires attention.

scholarly journals C-kit signaling promotes human pre-implantation 3PN embryonic development and blastocyst formation

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Jun Tan ◽  
Yang Zou ◽  
Zhi-Hui Huang ◽  
Zhi-Qin Zhang ◽  
Li-Ping Wu ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Embryo Culture ◽  
Culture System ◽  
Target Genes ◽  
Regulatory Mechanism ◽  
Control Group ◽  
Blastocyst Formation ◽  
High Quality ◽  
Immature Oocytes

Abstract Background Although in vitro culture system has been optimized in the past few decades, the problem of few or no high quality embryos has been still not completely solved. Accordingly, fully understanding the regulatory mechanism of pre-implantation embryonic development would be beneficial to further optimize the in vitro embryo culture system. Recent studies have found the expression of c-kit in mouse embryo and its promotion effects on mouse embryonic development. However, it is unclear the expression, the role and the related molecular regulatory mechanism of c-kit in human pre-implantation embryo development. Therefore, the present study is to determine whether c-kit is expressed in human pre-implantation embryos, and to investigate the possible regulatory mechanism of c-kit signaling in the process of embryonic development. Methods The present study includes human immature oocytes and three pronucleus (3PN) embryos collected from 768 women (28–32 ages) undergoing IVF, and normal 2PN embryos collected from ICR mice. Samples were distributed randomly into three different experimental groups: SCF group: G-1™ (medium for culture of embryos from the pro-nucleate stage to day 3) or G-2™ (medium for culture of embryos from day3 to blastocyst stage) + HSA (Human serum album) solution + rhSCF; SCF + imanitib (c-kit inhibitor) group: G-1™ or G-2™ + HSA solution + rhSCF + imanitib; SCF + U0126 (MEK/ERK inhibitor) group: G-1™ or G-2™ + HSA solution + rhSCF + U0126; Control group: G-1™ or G-2™ + HSA solution + PBS; The rate of good quality embryos at day 3, blastulation at day 6 and good quality blastulation at day 6 were analysis. RT-PCR, western blot and immunofluorescence staining were applied to detect the target genes and proteins in samples collected from human or mice, respectively. Results c-kit was expressed ubiquitously in all human immature oocytes, 3PN embryos and 3PN blastocysts. In the experiment of human 3PN embryos, compared with other groups, SCF group showed obviously higher rate of good quality at day 3, better rate of blastocyst formation at day 6 and higher rate of good quality blastocyst formation at day 6. Furthermore, we observed a higher ETV5 expression in SCF group than that in other groups. Similar results were also found in animal experiment. Interestingly, we also found a higher phosphorylation level of MEK/ERK signal molecule in mice embryos from SCF group than those from other groups. Moreover, inhibition of MEK/ERK signaling would remarkably impeded the mice embryonic development, which might be due to the reduced ETV5 expression. Conclusions The present study firstly revealed that c-kit signaling might promote the human pre-implantation embryonic development and blastocyst formation by up-regulating the expression of ETV5 via MEK/ERK pathway. Our findings provide a new idea for optimizing the in vitro embryo culture condition during ART program, which is beneficial to obtain high quality embryos for infertile patients.

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