scholarly journals Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

10.3791/50779 ◽  
2013 ◽  
Author(s):  
Jo-Ann McClure-Warnier ◽  
John M. Conly ◽  
Kunyan Zhang
Keyword(s):  
Staphylococcus Aureus ◽  
Multiplex Pcr ◽  
Pcr Assay ◽  
Methicillin Resistant ◽  
Multiplex Pcr Assay ◽  
Staphylococcal Cassette Chromosome

scholarly journals Characteristics of hospital- and community-acquired meticillin-resistant Staphylococcus aureus in Tehran, Iran

2014 ◽  
Vol 63 (6) ◽  
pp. 796-804 ◽  
Author(s):  
Fateh Rahimi ◽  
Mohammad Katouli ◽  
Mohammad Reza Pourshafie
Keyword(s):  
Staphylococcus Aureus ◽  
Multiplex Pcr ◽  
Outpatient Clinic ◽  
Pcr Assay ◽  
Type Iii ◽  
Multiplex Pcr Assay ◽  
Type 3 ◽  
Hospital Acquired ◽  
Staphylococcal Cassette Chromosome

Staphylococcus aureus is a leading cause of hospital-acquired (HA) and community-acquired (CA) infections worldwide. Recently, S. aureus strains resistant to meticillin (MRSA) have become established within both communities. We isolated 314 isolates of MRSA from hospitalized patients in a referral hospital (HA isolates) and 268 isolates from its outpatient clinic (CA isolates) in Tehran, Iran, between February 2008 and December 2010. These isolates were tested for their susceptibility to 17 antibiotics and typed using the PhPlate system. The diversity in the structures of staphylococcal cassette chromosome mec (SCCmec) elements and ccr types was also detected using a multiplex-PCR assay and isolates were examined for the presence of different classes of prophages. Whilst all isolates were resistant to penicillin, the HA isolates were significantly more resistant to all other antibiotics tested than the CA isolates. Isolates carrying only SCCmec type III and ccr type 3 were dominant (91 %), but 20 % of the CA isolates belonging to less prevalent types carried only SCCmec types IVa, c and ccr type 2. These isolates also carried pvl genes and contained SGA prophage type. Our results indicate that whilst the dominant clonal groups of HA- and CA-MRSA belong to SCCmec type III and carry ccr type 3 genes, several distinct but less prevalent types of CA-MRSA carrying SCCmec type IVa, c and type 2 ccr are also found in Tehran. These strains carry pvl genes and the SGA prophage type, a characteristic that might be used as a marker for detection of CA–MRSA in this country.

scholarly journals A simplified multiplex PCR assay for fast and easy discrimination of globally distributed staphylococcal cassette chromosome mec types in meticillin-resistant Staphylococcus aureus

2010 ◽  
Vol 59 (10) ◽  
pp. 1135-1139 ◽  
Author(s):  
Ehsanollah Ghaznavi-Rad ◽  
Mariana Nor Shamsudin ◽  
Zamberi Sekawi ◽  
Alex van Belkum ◽  
Vasanthakumari Neela
Keyword(s):  
Staphylococcus Aureus ◽  
Multiplex Pcr ◽  
Pcr Assay ◽  
Type Iii ◽  
Multiplex Pcr Assay ◽  
Mrsa Strains ◽  
Globally Distributed ◽  
Staphylococcal Cassette Chromosome

A multiplex PCR assay was developed for the identification of major types and subtypes of staphylococcal cassette chromosome mec (SCCmec) in meticillin-resistant Staphylococcus aureus (MRSA) strains. The method uses a novel 9 valent multiplex PCR plus two primer pairs for S. aureus identification and detection of meticillin resistance. All 389 clinical MRSA isolates from Malaysia and 18 European isolates from the Harmony collection harbouring different SCCmec types that we tested were correctly characterized by our PCR assay. SCCmec type III and V were by far the most common types among both hospital- and community-acquired Malaysian MRSA isolates, with an apparent emergence of MRSA harbouring the IVh type.

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