scholarly journals Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures

10.3791/50253 ◽  
2013 ◽  
Author(s):  
Bruce Yazejian ◽  
Rita M. Yazejian ◽  
Rachel Einarsson ◽  
Alan D. Grinnell
Keyword(s):  
Electrophysiological Recording ◽  
Nerve Muscle

Fate of sperm membrane in fertilized ova

1993 ◽  
Vol 51 ◽  
pp. 40-41
Author(s):  
Frank J. Longo
Keyword(s):  
Electron Microscopy ◽  
Electrical Activity ◽  
Sea Urchins ◽  
Morphological Changes ◽  
Integrated System ◽  
Electrophysiological Recording ◽  
Experimental Conditions ◽  
The Status ◽  
Sperm Membrane ◽  
Temporal And Spatial

Measurement of the egg's electrical activity, the fertilization potential or the activation current (in voltage clamped eggs), provides a means of detecting the earliest perceivable response of the egg to the fertilizing sperm. By using the electrical physiological record as a “real time” indicator of the instant of electrical continuity between the gametes, eggs can be inseminated with sperm at lower, more physiological densities, thereby assuring that only one sperm interacts with the egg. Integrating techniques of intracellular electrophysiological recording, video-imaging, and electron microscopy, we are able to identify the fertilizing sperm precisely and correlate the status of gamete organelles with the first indication (fertilization potential/activation current) of the egg's response to the attached sperm. Hence, this integrated system provides improved temporal and spatial resolution of morphological changes at the site of gamete interaction, under a variety of experimental conditions. Using these integrated techniques, we have investigated when sperm-egg plasma membrane fusion occurs in sea urchins with respect to the onset of the egg's change in electrical activity.

Nerve-Muscle Interaction

1979 ◽  
Vol 7 (5) ◽  
pp. 1169-1169
Author(s):  
S. WONNACOTT
Keyword(s):  
Nerve Muscle

Application and Evaluation of Case-Based Learning as a Part of Early Clinical Exposure for Undergraduate Medical Education Program

2020 ◽  
Vol 5 (08) ◽  
pp. 284-288
Author(s):  
Vandana Daulatabad ◽  
Prafull K. ◽  
Dr. Surekha S. Kadadi-Patil ◽  
Ramesh S. Patil
Keyword(s):  
Medical Education ◽  
Research Centre ◽  
Muscle Physiology ◽  
Case Scenario ◽  
Clinical Exposure ◽  
Clinical Scenarios ◽  
Case Based Learning ◽  
Nerve Muscle ◽  
Case Based ◽  
Early Clinical Exposure

Introduction: Medical Education is witnessing a significant transition and global shift towards competency based medical education (CBME) which includes early clinical exposure (ECE) program to help students apply and correlate principles of preclinical subjects with clinical scenarios, in various forms and in a variety of settings. One of the easy and feasible methods of ECE being Case Based Learning (CBL), our study aimed to design a case scenario and to evaluate impact of case base learning as a part of ECE module in first year undergraduate medical teaching program in nerve muscle physiology. Methods: The present study was conducted in 96 students at Ashwini Rural Medical College Hospital and Research Centre, Solapur after obtaining institutional ethics committee approval. 3 hrs session of CBL was conducted for a case scenario on myasthenia gravis in the nerve muscle physiology module. The students’ responses on pre-test, post-test and their insights regarding the CBL were taken through a pre validated questionnaire using 5-point Likert scale. Results: High impact of CBL was seen as significant improvement in student’s performance. Maximum students felt CBL to be easy method of learning and was highly appreciated through their feedback. Conclusion: CBL was found to have positive impact on understanding and perception of topic. CBL helped students to understand, evaluate, analyze, diagnose and interpret the case, paving them towards newer approach of self-directed and vertical integrated learning. CBL is easier, feasible an effective method among other early clinical exposure methods as it involves students in deeper and self-directed active learning, encouraging and promoting them to reach higher levels of cognitive domain of Bloom’s taxonomy. This method will be very useful in its practical implementation during online classes for ECE module in the threat of COVID 19 situation as well.

scholarly journals Implantable Optrode Array for Optogenetic Modulation and Electrical Neural Recording

Micromachines ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 725
Author(s):  
Saeyeong Jeon ◽  
Youjin Lee ◽  
Daeho Ryu ◽  
Yoon Kyung Cho ◽  
Yena Lee ◽  
...  
Keyword(s):  
Light Emitting Diode ◽  
Neural Recording ◽  
Electrophysiological Recording ◽  
Light Emitting ◽  
Technological Advances ◽  
Neuroscience Research ◽  
Cell Type Specific ◽  
Novel Design

During the last decade, optogenetics has become an essential tool for neuroscience research due to its unrivaled feature of cell-type-specific neuromodulation. There have been several technological advances in light delivery devices. Among them, the combination of optogenetics and electrophysiology provides an opportunity for facilitating optogenetic approaches. In this study, a novel design of an optrode array was proposed for realizing optical modulation and electrophysiological recording. A 4 × 4 optrode array and five-channel recording electrodes were assembled as a disposable part, while a reusable part comprised an LED (light-emitting diode) source and a power line. After the characterization of the intensity of the light delivered at the fiber tips, in vivo animal experiment was performed with transgenic mice expressing channelrhodopsin, showing the effectiveness of optical activation and neural recording.

Role of Muscle Activity in Nerve-Muscle Interaction in vitro

Nature ◽  
1974 ◽  
Vol 248 (5443) ◽  
pp. 70-71 ◽  
Author(s):  
J. H. STEINBACH
Keyword(s):  
Muscle Activity ◽  
Nerve Muscle

Effects of Nerve-Muscle Pedicle on Immobile Rat Vocal Folds in the Presence of Partial Innervation

2010 ◽  
Vol 119 (12) ◽  
pp. 823-829 ◽  
Author(s):  
Takashi Aoyama ◽  
Yoshihiko Kumai ◽  
Eiji Yumoto ◽  
Takaaki Ito ◽  
Satoru Miyamaru
Keyword(s):  
Vocal Folds ◽  
Nerve Muscle
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