scholarly journals Rapid Fibroblast Removal from High Density Human Embryonic Stem Cell Cultures

10.3791/3951 ◽  
2012 ◽  
Author(s):  
William S. Turner ◽  
Kara E. McCloskey
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Cultures ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
High Density

scholarly journals BCL-XL Mediates the Strong Selective Advantage of a 20q11.21 Amplification Commonly Found in Human Embryonic Stem Cell Cultures

2013 ◽  
Vol 1 (5) ◽  
pp. 379-386 ◽  
Author(s):  
Stuart Avery ◽  
Adam J. Hirst ◽  
Duncan Baker ◽  
Chin Yan Lim ◽  
Sharmini Alagaratnam ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Cultures ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Selective Advantage

scholarly journals Testing of nine different xeno-free culture media for human embryonic stem cell cultures

2007 ◽  
Vol 22 (5) ◽  
pp. 1231-1238 ◽  
Author(s):  
Kristiina Rajala ◽  
Heidi Hakala ◽  
Sarita Panula ◽  
Suvi Aivio ◽  
Harri Pihlajamäki ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Cultures ◽  
Human Embryonic Stem Cell ◽  
Culture Media ◽  
Embryonic Stem

scholarly journals A UTF1 -based selection system for stable homogeneously pluripotent human embryonic stem cell cultures

2007 ◽  
Vol 35 (18) ◽  
pp. e118 ◽  
Author(s):  
Shen Mynn Tan ◽  
Siew Tein Wang ◽  
Hannes Hentze ◽  
Peter Dröge
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Cultures ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Selection System

Population estimation of human embryonic stem cell cultures

2009 ◽  
pp. NA-NA
Author(s):  
Thomas Thurnherr ◽  
Andre Choo ◽  
Ivan Reading ◽  
Steve K. W. Oh
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Cultures ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Population Estimation

High-Density Polymer Microarrays: Identifying Synthetic Polymers that Control Human Embryonic Stem Cell Growth

2013 ◽  
Vol 3 (6) ◽  
pp. 848-853 ◽  
Author(s):  
Anne Hansen ◽  
Heidi K. Mjoseng ◽  
Rong Zhang ◽  
Michail Kalloudis ◽  
Vasileios Koutsos ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Growth ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Synthetic Polymers ◽  
High Density

scholarly journals Similar Population of CD133+ and DDX4+ VSEL-Like Stem Cells Sorted from Human Embryonic Stem Cell, Ovarian, and Ovarian Cancer Ascites Cell Cultures: The Real Embryonic Stem Cells?

Cells ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 706 ◽  
Author(s):  
Irma Virant-Klun ◽  
Petra Skerl ◽  
Srdjan Novakovic ◽  
Eda Vrtacnik-Bokal ◽  
Spela Smrkolj
Keyword(s):  
Ovarian Cancer ◽  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Embryonic Stem Cell ◽  
Cell Cultures ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Recurrent Ovarian Cancer

A population of small stem cells with diameters of up to 5 μm resembling very small embryonic-like stem cells (VSELs) were sorted from human embryonic stem cell (hESC) cultures using magnetic-activated cell sorting (MACS) based on the expression of a stem-cell-related marker prominin-1 (CD133). These VSEL-like stem cells had nuclei that almost filled the whole cell volume and expressed stem-cell-related markers (CD133, SSEA-4) and markers of germinal lineage (DDX4/VASA, PRDM14). They were comparable to similar populations of small stem cells sorted from cell cultures of normal ovaries and were the predominant cells in ascites of recurrent ovarian cancer. The sorted populations of CD133+ VSEL-like stem cells were quiescent in vitro, except for ascites, and were highly activated after exposure to valproic acid and follicle-stimulating hormone (FSH), indicating a new tool to study these cells in vitro. These VSEL-like stem cells spontaneously formed clusters resembling tumour-like structures or grew into larger, oocyte-like cells and were differentiated in vitro into adipogenic, osteogenic and neural lineages after sorting. We propose the population of VSEL-like stem cells from hESC cultures as potential original embryonic stem cells, which are present in the human embryo, persist in adult human ovaries from the embryonic period of life and are involved in cancer manifestation.

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