scholarly journals Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

10.3791/3796 ◽  
2012 ◽  
Author(s):  
Juan C. Garcia-Betancur ◽  
Ana Yepes ◽  
Johannes Schneider ◽  
Daniel Lopez
Keyword(s):  
Flow Cytometry ◽  
Bacillus Subtilis ◽  
Fluorescence Microscopy ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis

Electroporative Flow Cytometry for Single-Cell Analysis

2010 ◽  
pp. 125-142
Author(s):  
Chang Lu ◽  
Jun Wang ◽  
Ning Bao ◽  
Hsiang-Yu Wang
Keyword(s):  
Flow Cytometry ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis

Single Cell Analysis of Microalgae and Associated Bacteria Flora by Using Flow Cytometry

2021 ◽  
Vol 26 (6) ◽  
pp. 898-909
Author(s):  
Fabrizio Di Caprio ◽  
Simone Posani ◽  
Pietro Altimari ◽  
Alessandro Concas ◽  
Francesca Pagnanelli
Keyword(s):  
Flow Cytometry ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Associated Bacteria

Single‐Cell Analysis of Cytokine mRNA and Protein Expression by Flow Cytometry

2020 ◽  
Vol 92 (1) ◽  
Author(s):  
Rubina Pal ◽  
Jayne Schaubhut ◽  
Darcey Clark ◽  
Lynette Brown ◽  
Jennifer J. Stewart
Keyword(s):  
Flow Cytometry ◽  
Protein Expression ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Cytokine Mrna ◽  
Mrna And Protein Expression

Single‐Cell Analysis by Chemical Cytometry Combined with Fluorescence Microscopy

2004 ◽  
Vol 32 (1) ◽  
pp. 31-41 ◽  
Author(s):  
Kang Hu ◽  
Arash Zarrine‐Afsar ◽  
Hossein Ahmadzadeh ◽  
Sergey N. Krylov
Keyword(s):  
Fluorescence Microscopy ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Chemical Cytometry

Single-Cell Analysis of Morphological and Metabolic Heterogeneity in Euglena gracilis by Fluorescence-Imaging Flow Cytometry

2018 ◽  
Vol 90 (19) ◽  
pp. 11280-11289 ◽  
Author(s):  
Hector E. Muñoz ◽  
Ming Li ◽  
Carson T. Riche ◽  
Nao Nitta ◽  
Eric Diebold ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Single Cell ◽  
Fluorescence Imaging ◽  
Euglena Gracilis ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Imaging Flow Cytometry ◽  
Metabolic Heterogeneity

scholarly journals Single-cell analysis in situ in a Bacillus subtilis swarming community identifies distinct spatially separated subpopulations differentially expressing hag (flagellin), including specialized swarmers

Microbiology ◽  
2011 ◽  
Vol 157 (9) ◽  
pp. 2456-2469 ◽  
Author(s):  
Kassem Hamze ◽  
Sabine Autret ◽  
Krzysztof Hinc ◽  
Soumaya Laalami ◽  
Daria Julkowska ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Laboratory Strain ◽  
Cell Types ◽  
Spatiotemporal Pattern ◽  
Cell Analysis ◽  
Wide Range ◽  
Low Humidity

The non-domesticated Bacillus subtilis strain 3610 displays, over a wide range of humidity, hyper-branched, dendritic, swarming-like migration on a minimal agar medium. At high (70 %) humidity, the laboratory strain 168 sfp + (producing surfactin) behaves very similarly, although this strain carries a frameshift mutation in swrA, which another group has shown under their conditions (which include low humidity) is essential for swarming. We reconcile these different results by demonstrating that, while swrA is essential for dendritic migration at low humidity (30–40 %), it is dispensable at high humidity. Dendritic migration (flagella- and surfactin-dependent) of strains 168 sfp + swrA and 3610 involves elongation of dendrites for several hours as a monolayer of cells in a thin fluid film. This enabled us to determine in situ the spatiotemporal pattern of expression of some key players in migration as dendrites develop, using gfp transcriptional fusions for hag (encoding flagellin), comA (regulation of surfactin synthesis) as well as eps (exopolysaccharide synthesis). Quantitative (single-cell) analysis of hag expression in situ revealed three spatially separated subpopulations or cell types: (i) networks of chains arising early in the mother colony (MC), expressing eps but not hag; (ii) largely immobile cells in dendrite stems expressing intermediate levels of hag; and (iii) a subpopulation of cells with several distinctive features, including very low comA expression but hyper-expression of hag (and flagella). These specialized cells emerge from the MC to spearhead the terminal 1 mm of dendrite tips as swirling and streaming packs, a major characteristic of swarming migration. We discuss a model for this swarming process, emphasizing the importance of population density and of the complementary roles of packs of swarmers driving dendrite extension, while non-mobile cells in the stems extend dendrites by multiplication.

scholarly journals Droplet flow cytometry for single-cell analysis

RSC Advances ◽  
2021 ◽  
Vol 11 (34) ◽  
pp. 20944-20960
Author(s):  
Ming Li ◽  
Hangrui Liu ◽  
Siyuan Zhuang ◽  
Keisuke Goda
Keyword(s):  
Flow Cytometry ◽  
Single Cell ◽  
High Throughput ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Droplet Flow ◽  
Recent Advances

This work reviews recent advances in the integration of emulsion microdroplets and flow cytometry technologies, so-called droplet flow cytometry (DFC), for high-throughput single-cell analysis.

Single-cell analysis of the murine chemokines MIP-1α, MIP-1β, RANTES and ATAC/lymphotactin by flow cytometry

2003 ◽  
Vol 274 (1-2) ◽  
pp. 83-91 ◽  
Author(s):  
Brigitte G Dorner ◽  
Sabine Steinbach ◽  
Martin B Hüser ◽  
Richard A Kroczek ◽  
Alexander Scheffold
Keyword(s):  
Flow Cytometry ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis
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