scholarly journals The Neuroblast Assay: An Assay for the Generation and Enrichment of Neuronal Progenitor Cells from Differentiating Neural Stem Cell Progeny Using Flow Cytometry

10.3791/3712 ◽  
2012 ◽  
Author(s):  
Hassan Azari ◽  
Sharareh Sharififar ◽  
Jeff M. Fortin ◽  
Brent A. Reynolds
Keyword(s):  
Flow Cytometry ◽  
Stem Cell ◽  
Progenitor Cells ◽  
Neural Stem Cell ◽  
Neuronal Progenitor Cells ◽  
Neuronal Progenitor

scholarly journals A selective cytotoxic adenovirus vector for concentration of pluripotent stem cells in human pluripotent stem cell-derived neural progenitor cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Takamasa Hirai ◽  
Ken Kono ◽  
Rumi Sawada ◽  
Takuya Kuroda ◽  
Satoshi Yasuda ◽  
...  
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Stem Cell ◽  
Progenitor Cells ◽  
Pluripotent Stem Cells ◽  
Pluripotent Stem Cell ◽  
Neural Progenitor Cells ◽  
Neural Progenitor ◽  
Sensitive Detection ◽  
Quality And Safety

AbstractHighly sensitive detection of residual undifferentiated pluripotent stem cells is essential for the quality and safety of cell-processed therapeutic products derived from human induced pluripotent stem cells (hiPSCs). We previously reported the generation of an adenovirus (Ad) vector and adeno-associated virus vectors that possess a suicide gene, inducible Caspase 9 (iCasp9), which makes it possible to sensitively detect undifferentiated hiPSCs in cultures of hiPSC-derived cardiomyocytes. In this study, we investigated whether these vectors also allow for detection of undifferentiated hiPSCs in preparations of hiPSC-derived neural progenitor cells (hiPSC-NPCs), which have been expected to treat neurological disorders. To detect undifferentiated hiPSCs, the expression of pluripotent stem cell markers was determined by immunostaining and flow cytometry. Using immortalized NPCs as a model, the Ad vector was identified to be the most efficient among the vectors tested in detecting undifferentiated hiPSCs. Moreover, we found that the Ad vector killed most hiPSC-NPCs in an iCasp9-dependent manner, enabling flow cytometry to detect undifferentiated hiPSCs intermingled at a lower concentration (0.002%) than reported previously (0.1%). These data indicate that the Ad vector selectively eliminates hiPSC-NPCs, thus allowing for sensitive detection of hiPSCs. This cytotoxic viral vector could contribute to ensuring the quality and safety of hiPSCs-NPCs for therapeutic use.

scholarly journals RNase A Promotes Proliferation of Neuronal Progenitor Cells via an ERK-Dependent Pathway

2018 ◽  
Vol 11 ◽  
Author(s):  
Hsin-Yu Liu ◽  
Chiung-Ya Chen ◽  
Yun-Fen Hung ◽  
Hong-Ru Lin ◽  
Hsu-Wen Chao ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Rnase A ◽  
Neuronal Progenitor Cells ◽  
Neuronal Progenitor ◽  
Dependent Pathway

scholarly journals cAMP promotes differentiation of rodent neuronal progenitor cells

2011 ◽  
Vol 1 (1) ◽  
pp. 9 ◽  
Author(s):  
Guilherme Lepski ◽  
Cinthia Elim Jannes ◽  
Guido Nikkhah
Keyword(s):  
Progenitor Cells ◽  
Neuronal Progenitor Cells ◽  
Neuronal Progenitor

scholarly journals Apolipoprotein E4 Suppresses Neuronal-Specific Gene Expression in Maturing Neuronal Progenitor Cells Exposed to HIV

2017 ◽  
Vol 12 (3) ◽  
pp. 462-483 ◽  
Author(s):  
Rebeca Geffin ◽  
Ricardo Martinez ◽  
Alicia de las Pozas ◽  
Biju Issac ◽  
Micheline McCarthy
Keyword(s):  
Gene Expression ◽  
Progenitor Cells ◽  
Specific Gene ◽  
Apolipoprotein E4 ◽  
Specific Gene Expression ◽  
Neuronal Progenitor Cells ◽  
Neuronal Progenitor

Metabolic glycan labeling-assisted discovery of cell-surface markers for primary neural stem and progenitor cells

2018 ◽  
Vol 54 (43) ◽  
pp. 5486-5489
Author(s):  
Qing-Ran Bai ◽  
Lu Dong ◽  
Yi Hao ◽  
Xing Chen ◽  
Qin Shen
Keyword(s):  
Mass Spectrometry ◽  
Stem Cell ◽  
Cell Surface ◽  
Progenitor Cells ◽  
Neural Stem Cell ◽  
Surface Markers ◽  
Metabolic Labeling ◽  
Cell Surface Markers ◽  
Stem And Progenitor Cells

Metabolic labeling with azidosugars in a neural stem cell (NSC)-enriched endothelial coculture followed by mass-spectrometry profiling identifies sialoglycoproteins on NSCs.

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