scholarly journals Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-cultures from Post-natal Murine Tissues

10.3791/3324 ◽  
2011 ◽  
Author(s):  
Ryan W. O'Meara ◽  
Scott D. Ryan ◽  
Holly Colognato ◽  
Rashmi Kothary
Keyword(s):  
Oligodendrocyte Cultures

625 Establishment of stage-specific pure oligodendrocyte cultures and oligodendrocyte lineage

1997 ◽  
Vol 28 ◽  
pp. S91
Author(s):  
Y. Sakurai ◽  
D. Nishimura ◽  
S. Kawato ◽  
T. Hamada ◽  
H. Yamanouchi ◽  
...  
Keyword(s):  
Oligodendrocyte Cultures

Developmental regulation of α1A-adrenoceptor function in rat brain oligodendrocyte cultures

2002 ◽  
Vol 42 (5) ◽  
pp. 685-696 ◽  
Author(s):  
Amani Khorchid ◽  
Qiaoling Cui ◽  
Eduardo Molina-Holgado ◽  
Guillermina Almazan
Keyword(s):  
Rat Brain ◽  
Developmental Regulation ◽  
Oligodendrocyte Cultures

scholarly journals A role for TGF-beta in oligodendrocyte differentiation.

1993 ◽  
Vol 121 (6) ◽  
pp. 1397-1407 ◽  
Author(s):  
R D McKinnon ◽  
G Piras ◽  
J A Ida ◽  
M Dubois-Dalcq
Keyword(s):  
Cell Proliferation ◽  
Progenitor Cells ◽  
Progenitor Cell ◽  
Tgf Beta ◽  
Oligodendrocyte Differentiation ◽  
Progenitor Cell Proliferation ◽  
Glial Progenitor Cells ◽  
Oligodendrocyte Development ◽  
Oligodendrocyte Cultures

Oligodendrocyte-type-2 astrocyte (O-2A) glial progenitor cells undergo a limited number of mitotic divisions in response to PDGF before differentiating into oligodendrocytes, the myelin-forming cell of the CNS. We examined the mechanism limiting O-2A proliferation, and demonstrate that these cells secrete an inhibitor of cell proliferation that can be neutralized with antibodies to TGF-beta. O-2A cells also secrete an inhibitory activity that cannot be neutralized with TGF-beta antibodies. O-2A progenitor cultures express TGF-beta 1 isoform and its transcript, while oligodendrocyte cultures express TGF-beta 1, beta-2, and beta-3 isoforms. Both recombinant TGF-beta 1 and O-2A conditioned medium inhibit the proliferation of O-2A progenitor cells cultured in the presence of PDGF, and this inhibition can be partially neutralized with polyclonal TGF-beta antibodies. Thus, TGF-beta produced by O-2A cells may limit PDGF-driven mitosis and promote oligodendrocyte development. TGF-beta is a less potent inhibitor of O-2A proliferation when these cells are cultured in the presence of bFGF, suggesting that bFGF interferes with TGF-beta signaling. Thus, the production of TGF-beta by cells in the O-2A lineage may account for the distinct effects of PDGF and bFGF on O-2A progenitor cell proliferation. Moreover, our results suggest that TGF-beta may be an important mediator of oligodendrocyte differentiation.

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