scholarly journals Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

10.3791/3322 ◽  
2011 ◽  
Author(s):  
Jerry Zhou ◽  
Larissa Belov ◽  
Michael J. Solomon ◽  
Charles Chan ◽  
Stephen J. Clarke ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Surface ◽  
Cancer Cell ◽  
Surface Protein ◽  
Protein Profiling ◽  
Colorectal Cancer Cell ◽  
Cell Surface Protein ◽  
Antibody Microarray

scholarly journals Surface-bound galectin-4 regulates gene transcription and secretion of chemokines in human colorectal cancer cell lines

Tumor Biology ◽  
2017 ◽  
Vol 39 (3) ◽  
pp. 101042831769168 ◽  
Author(s):  
U Subrahmanyeswara Rao ◽  
Prema S Rao
Keyword(s):  
Colorectal Cancer ◽  
Cell Proliferation ◽  
Cell Surface ◽  
Cancer Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Colorectal Cancer Cell ◽  
Colorectal Tumorigenesis ◽  
Colorectal Cancer Cells ◽  
Colorectal Cancer Cell Lines

One long-term complication of chronic intestinal inflammation is the development of colorectal cancer. However, the mechanisms linking inflammation to the colorectal tumorigenesis are poorly defined. Previously, we have demonstrated that galectin-4 is predominantly expressed in the luminal epithelia of the gastrointestinal tract, and its loss of expression plays a key role in the colorectal tumorigenesis. However, the mechanism by which galectin-4 regulates inflammation-induced tumorigenesis is unclear. Here, we show that galectin-4 secreted by the colorectal cancer cell lines was bound to the cell surface. Neutralization of surface-bound galectin-4 with anti-galectin-4 antibody resulted in increased cell proliferation with concomitant secretion of several chemokines into the extracellular medium. Neutralization of the surface-bound galectin-4 also resulted in the up-regulation of transcription of 29 genes, several of which are components of multiple inflammation signaling pathways. In an alternate experiment, binding of recombinant galectin-4 protein to cell surface of the galectin-4-negative colorectal cancer cells resulted in increased p27, and decreased cyclin D1 and c-Myc levels, leading to cell cycle arrest and apoptosis. Together, these data demonstrated that surface-bound galectin-4 is a dual function protein—down-regulating cell proliferation and chemokine secretion in galectin-4-expressing colorectal cancer cells on one hand and inducing apoptosis in galectin-4-negative colorectal cancer cells on the other hand.

scholarly journals Comprehensive Cell Surface Protein Profiling Identifies Specific Markers of Human Naive and Primed Pluripotent States

2017 ◽  
Vol 20 (6) ◽  
pp. 874-890.e7 ◽  
Author(s):  
Amanda J. Collier ◽  
Sarita P. Panula ◽  
John Paul Schell ◽  
Peter Chovanec ◽  
Alvaro Plaza Reyes ◽  
...  
Keyword(s):  
Cell Surface ◽  
Surface Protein ◽  
Protein Profiling ◽  
Cell Surface Protein

scholarly journals Facile Discovery of Cell-Surface Protein Targets of Cancer Cell Aptamers

2015 ◽  
Vol 14 (10) ◽  
pp. 2692-2700 ◽  
Author(s):  
Tao Bing ◽  
Dihua Shangguan ◽  
Yinsheng Wang
Keyword(s):  
Cell Surface ◽  
Cancer Cell ◽  
Surface Protein ◽  
Cell Surface Protein ◽  
Protein Targets

scholarly journals Cell-Surface Protein Profiling Identifies Distinctive Markers of Progenitor Cells in Human Skeletal Muscle

2016 ◽  
Vol 7 (2) ◽  
pp. 263-278 ◽  
Author(s):  
Akiyoshi Uezumi ◽  
Masashi Nakatani ◽  
Madoka Ikemoto-Uezumi ◽  
Naoki Yamamoto ◽  
Mitsuhiro Morita ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cell Surface ◽  
Progenitor Cells ◽  
Human Skeletal Muscle ◽  
Surface Protein ◽  
Protein Profiling ◽  
Cell Surface Protein

Immunogold and immunoblot studies on a cell surface protein found in primary mesenchyme cells and in the cortical secretory vesicles of the sea urchin egg

1987 ◽  
Vol 45 ◽  
pp. 832-833
Author(s):  
G.L. Decker ◽  
M.C. Valdizan
Keyword(s):  
Cell Surface ◽  
Sea Urchin ◽  
Surface Protein ◽  
Egg Cell ◽  
Secretory Vesicles ◽  
Cell Surface Protein ◽  
Surface Complexes ◽  
Mesenchyme Cells ◽  
Lowicryl K4m ◽  
Primary Mesenchyme Cells

A monoclonal antibody designated MAb 1223 has been used to show that primary mesenchyme cells of the sea urchin embryo express a 130-kDa cell surface protein that may be directly involved in Ca2+ uptake required for growth of skeletal spicules. Other studies from this laboratory have shown that the 1223 antigen, although in relatively low abundance, is also expressed on the cell surfaces of unfertilized eggs and on the majority of blastomeres formed prior to differentiation of the primary mesenchyme cells.We have studied the distribution of 1223 antigen in S. purpuratus eggs and embryos and in isolated egg cell surface complexes that contain the cortical secretory vesicles. Specimens were fixed in 1.0% paraformaldehyde and 1.0% glutaraldehyde and embedded in Lowicryl K4M as previously reported. Colloidal gold (8nm diameter) was prepared by the method of Mulpfordt.

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