scholarly journals Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation

10.3791/3087 ◽  
2011 ◽  
Author(s):  
Maria L. Lombardi ◽  
Monika Zwerger ◽  
Jan Lammerding
Keyword(s):  
Mechanical Properties ◽  
Cell Nucleus ◽  
Interphase Cell ◽  
Substrate Strain

Tensile shear strength of UF- and MUF-bonded veneer related to data of adhesives and cell walls measured by nanoindentation

Holzforschung ◽  
2010 ◽  
Vol 64 (3) ◽  
Author(s):  
Frank Stöckel ◽  
Johannes Konnerth ◽  
Wolfgang Kantner ◽  
Johann Moser ◽  
Wolfgang Gindl
Keyword(s):  
Mechanical Properties ◽  
Shear Strength ◽  
Bond Strength ◽  
Cell Walls ◽  
Urea Formaldehyde ◽  
Lap Joints ◽  
Tensile Shear Strength ◽  
Tensile Shear ◽  
Interphase Cell ◽  
Cure Time

Abstract The tensile shear strength of veneer lap joints was characterised. The joints were produced with an Automated Bonding Evaluation System (ABES) using urea-formaldehyde (UF) as well as melamine-urea-formaldehyde (MUF) adhesive formulated for particleboard production. At a fixed heating temperature of 110°C, a systematic increase in bond strength was observed for both adhesives with increasing cure time. The absolute bond strength was significantly higher for MUF compared to UF. Nanoindentation experiments with the same specimens used for ABES revealed a very hard, stiff and brittle character of the UF resin, whereas the MUF proved significantly less hard and stiff, and less brit-tle. Wood cell walls in contact with adhesive, i.e., where adhesive penetration into the cell wall was assumed, showed significantly altered mechanical properties. Such cell walls were harder, stiffer and more brittle than unaffected reference cell walls. These effects were slightly more pronounced for UF than for MUF. Comparing UF and MUF, the micro-mechanical properties of cured adhesive and interphase cell walls confirm earlier observations that tougher adhesives can lead to higher macroscopic bond strength. In strong contrast to that, no obvious correlation was found between micromechanical properties and the strong cure time dependence of macroscopic bond strength.

STUDYING THE LOCAL MECHANICAL PROPERTIES OF LIVING CELLS VIA SCANNING ION-CONDUCTING MICROSCOPY

2020 ◽  
pp. 154-156
Author(s):  
A. Vaneev ◽  
N. Savin ◽  
A. Yakovlev ◽  
A. Alova ◽  
S. Lavrushkina ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Mechanical Properties ◽  
Actin Cytoskeleton ◽  
Tumor Cells ◽  
Cell Nucleus ◽  
Living Cells ◽  
Mechanisms Of Action ◽  
Human Prostate Cancer ◽  
Before And After ◽  
Ion Conducting

The mechanical properties of PC-3 tumor cells of human prostate cancer before and after exposure to substances acting on the actin cytoskeleton, microtubules, and cell nucleus were measured by scanning ion-conducting microscopy. Changes in local mechanical properties corresponding to the mechanisms of action of these substances were found

scholarly journals Modeling diffusional transport in the interphase cell nucleus

2007 ◽  
Vol 127 (4) ◽  
pp. 045102 ◽  
Author(s):  
Annika Wedemeier ◽  
Holger Merlitz ◽  
Chen-Xu Wu ◽  
Jörg Langowski
Keyword(s):  
Cell Nucleus ◽  
Interphase Cell

scholarly journals Mapping replicational sites in the eucaryotic cell nucleus.

1989 ◽  
Vol 108 (1) ◽  
pp. 1-11 ◽  
Author(s):  
H Nakayasu ◽  
R Berezney
Keyword(s):  
Cell Nucleus ◽  
Nuclear Matrix ◽  
Nuclear Dna ◽  
Fluorescent Microscopy ◽  
Mouse Fibroblast ◽  
Type I ◽  
Interphase Cell ◽  
Permeabilized Cells ◽  
Replication Sites

We have used fluorescent microscopy to map DNA replication sites in the interphase cell nucleus after incorporation of biotinylated dUTP into permeabilized PtK-1 kangaroo kidney or 3T3 mouse fibroblast cells. Discrete replication granules were found distributed throughout the nuclear interior and along the periphery. Three distinct patterns of replication sites in relationship to chromatin domains in the cell nucleus and the period of S phase were detected and termed type I (early to mid S), type II (mid to late S) and type III (late S). Similar patterns were seen with in vivo replicated DNA using antibodies to 5-bromodeoxyuridine. Extraction of the permeabilized cells with DNase I and 0.2 M ammonium sulfate revealed a striking maintenance of these replication granules and their distinct intranuclear arrangements with the remaining nuclear matrix structures despite the removal of greater than 90% of the total nuclear DNA. The in situ prepared nuclear matrix structures also incorporated biotinylated dUTP into replication granules that were indistinguishable from those detected within the intact nucleus.

scholarly journals Nuclear lamin isoforms differentially contribute to LINC complex-dependent nucleocytoskeletal coupling and whole cell mechanics

2021 ◽  
Author(s):  
Amir Vahabikashi ◽  
Suganya Sivagurunathan ◽  
Fiona Ann Sadsad Nicdao ◽  
Yu Long Han ◽  
Chan Young Park ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Cell Nucleus ◽  
Cell Mechanics ◽  
Linc Complex ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts ◽  
Nuclear Mechanics ◽  
Cellular Volume ◽  
Nuclear Lamin ◽  
A Cell

The ability of a cell to regulate its mechanical properties is central to its function. Emerging evidence suggests that interactions between the cell nucleus and cytoskeleton influence cell mechanics through poorly understood mechanisms. Here we show that A- and B-type nuclear lamin isoforms distinctively modulate both nuclear and cellular volume and selectively stabilize the linker of nucleoskeleton and cytoskeleton (LINC) complexes that couple the nucleus to cytoskeletal actin and vimentin. We reveal, further, that loss of each of the four-known lamin isoforms in the mouse embryonic fibroblasts differentially affects cortical and cytoplasmic stiffness as well as cellular contractility, and then propose a LINC complex mediated model that explains these impaired mechanical phenotypes. Finally, we demonstrate that loss of each lamin isoform softens the nucleus in a manner that correlates with loss of heterochromatin. Together, these findings uncover distinctive roles for each lamin isoform in maintaining cellular and nuclear mechanics.

Lamin A as a Determinant of Mechanical Properties of the Cell Nucleus in Health and Disease

2021 ◽  
Vol 86 (10) ◽  
pp. 1288-1300
Author(s):  
Natalia L. Ovsiannikova ◽  
Svetlana V. Lavrushkina ◽  
Anastasia V. Ivanova ◽  
Ludmila M. Mazina ◽  
Oxana A. Zhironkina ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Cell Nucleus ◽  
Lamin A ◽  
Health And Disease

scholarly journals Mechanical Properties of the Cell Nucleus and the Effect of Emerin Deficiency

2006 ◽  
Vol 91 (12) ◽  
pp. 4649-4664 ◽  
Author(s):  
A.C. Rowat ◽  
J. Lammerding ◽  
J.H. Ipsen
Keyword(s):  
Mechanical Properties ◽  
Cell Nucleus
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