scholarly journals Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

10.3791/3061 ◽  
2011 ◽  
Author(s):  
Patrick J. M. Murphy ◽  
Morgan Shannon ◽  
John Goertz
Keyword(s):  
Atomic Force Microscopy ◽  
Recombinant Dna ◽  
Protein Complexes ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Visualization and identification of hepatitis c viral particles by atomic force microscopy combined with ms/ms analysis

2010 ◽  
Vol 56 (1) ◽  
pp. 26-39 ◽  
Author(s):  
A.L. Kaysheva ◽  
Yu.D. Ivanov ◽  
V.G. Zgoda ◽  
P.A. Frantsuzov ◽  
T.O. Pleshakova ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Hepatitis C ◽  
Protein Complexes ◽  
Mass Spectrometric ◽  
Force Microscopy ◽  
Atomic Force ◽  
Spectrometric Identification ◽  
Detection And Identification ◽  
Viral Particles ◽  
Mass Spectrometric Identification

Possibility of detection and identification of hepatitis C viral particles with mass spectrometry (MS) in combination with atomic force microscopy (AFM) had been investigated. AFM/MS approach is based on two technologies: 1. AFM-biospecific fishing that allows to detect, concentrate from solution and to count protein complexes on a surface of AFM-nanochip; 2. mass spectrometric identification of these complexes. AFM-biospecific fishing of HCVcoreAg from solution was carried onto surface of AFM-nanochips with immobilized anti-HCVcoreAg. It was shown that HCVcoreAg/anti-HCVcoreim complexes were formed onto AFM-nanochips in quantity sufficient for mass spectrometric identification. Thus, AFM/MS approach allows to identify fragments of hepatitis C virus fished onto a surface of AFM-nanochip from serum.

Atomic Force Microscopy on RNA-Protein Complexes related to RNAi

2005 ◽  
Vol 2005 (Fall) ◽  
Author(s):  
Nils Anspach ◽  
Blagovesta Popova ◽  
Christian Hammann ◽  
Lukasz Jaskiewicz ◽  
Witold Filipowicz ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Protein Complexes ◽  
Force Microscopy ◽  
Atomic Force ◽  
Rna Protein Complexes

scholarly journals Atomic force microscopy of DNA-colloidal gold and DNA-protein complexes

1993 ◽  
Author(s):  
Luming Niu ◽  
Wenling Shaiu ◽  
James Vesenka ◽  
Drena D. Larson ◽  
Eric Henderson
Keyword(s):  
Atomic Force Microscopy ◽  
Colloidal Gold ◽  
Protein Complexes ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Single-Molecule Dynamics of the DNA−EcoRII Protein Complexes Revealed with High-Speed Atomic Force Microscopy

Biochemistry ◽  
2009 ◽  
Vol 48 (44) ◽  
pp. 10492-10498 ◽  
Author(s):  
Jamie L. Gilmore ◽  
Yuki Suzuki ◽  
Gintautas Tamulaitis ◽  
Virginijus Siksnys ◽  
Kunio Takeyasu ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Single Molecule ◽  
High Speed ◽  
Protein Complexes ◽  
Force Microscopy ◽  
Atomic Force ◽  
Single Molecule Dynamics ◽  
Molecule Dynamics

scholarly journals Accurate nanoscale flexibility measurement of DNA and DNA–protein complexes by atomic force microscopy in liquid

Nanoscale ◽  
2017 ◽  
Vol 9 (31) ◽  
pp. 11327-11337 ◽  
Author(s):  
Divakaran Murugesapillai ◽  
Serge Bouaziz ◽  
L. James Maher ◽  
Nathan E. Israeloff ◽  
Craig E. Cameron ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Optical Tweezers ◽  
Protein Complexes ◽  
Persistence Length ◽  
Three Dimensional ◽  
Force Microscopy ◽  
Atomic Force ◽  
Afm Imaging ◽  
Flexibility Measurement ◽  
Length Measurements

We obtain accurate three-dimensional persistence length measurements for DNA and DNA–protein complexes using liquid AFM imaging, validated by optical tweezers.

Atomic force microscopy with carbon nanotube probe resolves the subunit organization of protein complexes

2000 ◽  
Vol 49 (3) ◽  
pp. 415-421 ◽  
Author(s):  
K. I. Hohmura ◽  
Y. Itokazu ◽  
S. H. Yoshimura ◽  
G. Mizuguchi ◽  
Y.-s. Masamura ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Carbon Nanotube ◽  
Protein Complexes ◽  
Force Microscopy ◽  
Atomic Force ◽  
Subunit Organization
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