scholarly journals Heterokaryon Technique for Analysis of Cell Type-specific Localization

10.3791/2488 ◽  
2011 ◽  
Author(s):  
Roseann Gammal ◽  
Krista Baker ◽  
Destin Heilman
Keyword(s):  
Cell Type ◽  
Specific Localization ◽  
Cell Type Specific

scholarly journals Quantitative synapse analysis for cell-type specific connectomics

2018 ◽  
Author(s):  
Dika A. Kuljis ◽  
Khaled Zemoura ◽  
Cheryl A. Telmer ◽  
Jiseok Lee ◽  
Eunsol Park ◽  
...  
Keyword(s):  
Large Scale ◽  
Neural Circuit ◽  
Apical Dendrite ◽  
Automated Detection ◽  
Cell Type ◽  
Disease States ◽  
Specific Localization ◽  
Cell Type Specific ◽  
Dendritic Branches ◽  
Circuit Function

AbstractAnatomical methods for determining cell-type specific connectivity are essential to inspire and constrain our understanding of neural circuit function. We developed new genetically-encoded reagents for fluorescence-synapse labeling and connectivity analysis in brain tissue, using a fluorogen-activating protein (FAP)-or YFP-coupled, postsynaptically-localized neuroligin-1 targeting sequence (FAP/YFPpost). Sparse viral expression of FAP/YFPpost with the cell-filling, red fluorophore dTomato (dTom) enabled high-throughput, compartment-specific localization of synapses across diverse neuron types in mouse somatosensory cortex. High-resolution confocal image stacks of virally-transduced neurons were used for 3D reconstructions of postsynaptic cells and automated detection of synaptic puncta. We took advantage of the bright, far-red emission of FAPpost puncta for multichannel fluorescence alignment of dendrites, synapses, and presynaptic neurites to assess subtype-specific inhibitory connectivity onto L2 neocortical pyramidal (Pyr) neurons. Quantitative and compartment-specific comparisons show that PV inputs are the dominant source of inhibition at both the soma and across all dendritic branches examined and were particularly concentrated at the primary apical dendrite, a previously unrecognized compartment of L2 Pyr neurons. Our fluorescence-based synapse labeling reagents will facilitate large-scale and cell-type specific quantitation of changes in synaptic connectivity across development, learning, and disease states.

scholarly journals Correction to: Cell type-specific localization of Ephs pairing with ephrin-B2 in the rat postnatal pituitary gland

2017 ◽  
Vol 371 (2) ◽  
pp. 385-387
Author(s):  
Saishu Yoshida ◽  
Takako Kato ◽  
Naoko Kanno ◽  
Naoto Nishimura ◽  
Hiroto Nishihara ◽  
...  
Keyword(s):  
Pituitary Gland ◽  
Cell Type ◽  
Specific Localization ◽  
Cell Type Specific

Cell type-specific localization of Ephs pairing with ephrin-B2 in the rat postnatal pituitary gland

2017 ◽  
Vol 370 (1) ◽  
pp. 99-112 ◽  
Author(s):  
Saishu Yoshida ◽  
Takako Kato ◽  
Naoko Kanno ◽  
Naoto Nishimura ◽  
Hiroto Nishihara ◽  
...  
Keyword(s):  
Pituitary Gland ◽  
Cell Type ◽  
Specific Localization ◽  
Cell Type Specific

The Stage- and Cell Type-Specific Localization of Fragile X Mental Retardation Protein in Rat Ovaries

2015 ◽  
Vol 22 (12) ◽  
pp. 1524-1529 ◽  
Author(s):  
Noriyuki Takahashi ◽  
Wataru Tarumi ◽  
Masanori T. Itoh ◽  
Bunpei Ishizuka
Keyword(s):  
Mental Retardation ◽  
Fragile X ◽  
Cell Type ◽  
Fragile X Mental Retardation ◽  
Mental Retardation Protein ◽  
Specific Localization ◽  
Cell Type Specific

scholarly journals Heterogeneity and Cell Type-Specific Localization of a Cell Wall Glycoprotein from Carrot Suspension Cells

1991 ◽  
Vol 96 (3) ◽  
pp. 705-712 ◽  
Author(s):  
Fred A. van Engelen ◽  
Peter Sterk ◽  
Hilbert Booij ◽  
Jan H.G. Cordewener ◽  
Wim Rook ◽  
...  
Keyword(s):  
Cell Wall ◽  
Cell Type ◽  
Suspension Cells ◽  
Specific Localization ◽  
A Cell ◽  
Cell Type Specific

scholarly journals Cell type specific localization of sphingomyelin biosynthesis

FEBS Letters ◽  
2000 ◽  
Vol 478 (1-2) ◽  
pp. 9-12 ◽  
Author(s):  
Farsaneh Sadeghlar ◽  
Konrad Sandhoff ◽  
Gerhild van Echten-Deckert
Keyword(s):  
Cell Type ◽  
Specific Localization ◽  
Cell Type Specific
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