scholarly journals Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons

10.3791/2373 ◽  
2011 ◽  
Author(s):  
Christopher Viesselmann ◽  
Jason Ballweg ◽  
Derek Lumbard ◽  
Erik W. Dent
Keyword(s):  
Primary Culture ◽  
Cortical Neurons ◽  
Embryonic Mouse

VIP actions on cellular transduction mechanisms in striatal and cortical neurons in primary culture

1985 ◽  
Vol 10 ◽  
pp. S35
Author(s):  
Samuel Welss ◽  
Sebben Michèle ◽  
DorothyE. Kemp ◽  
Fritz Sladeczek ◽  
Joël Bockaert
Keyword(s):  
Primary Culture ◽  
Cortical Neurons ◽  
Transduction Mechanisms

scholarly journals The NKCC1 antagonist bumetanide mitigates interneuronopathy associated with ethanol exposure in utero

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Alexander GJ Skorput ◽  
Stephanie M Lee ◽  
Pamela WL Yeh ◽  
Hermes H Yeh
Keyword(s):  
Prefrontal Cortex ◽  
Cortical Neurons ◽  
Fetal Alcohol Spectrum Disorders ◽  
Behavioral Flexibility ◽  
Ethanol Exposure ◽  
Embryonic Mouse ◽  
Form And Function ◽  
Tangential Migration ◽  
And Function

Prenatal exposure to ethanol induces aberrant tangential migration of corticopetal GABAergic interneurons, and long-term alterations in the form and function of the prefrontal cortex. We have hypothesized that interneuronopathy contributes significantly to the pathoetiology of fetal alcohol spectrum disorders (FASD). Activity-dependent tangential migration of GABAergic cortical neurons is driven by depolarizing responses to ambient GABA present in the cortical enclave. We found that ethanol exposure potentiates the depolarizing action of GABA in GABAergic cortical interneurons of the embryonic mouse brain. Pharmacological antagonism of the cotransporter NKCC1 mitigated ethanol-induced potentiation of GABA depolarization and prevented aberrant patterns of tangential migration induced by ethanol in vitro. In a model of FASD, maternal bumetanide treatment prevented interneuronopathy in the prefrontal cortex of ethanol exposed offspring, including deficits in behavioral flexibility. These findings position interneuronopathy as a mechanism of FASD symptomatology, and posit NKCC1 as a pharmacological target for the management of FASD.

Cellular migration patterns in the developing mouse cerebral cortex

Development ◽  
1990 ◽  
Vol 110 (3) ◽  
pp. 713-732 ◽  
Author(s):  
C.P. Austin ◽  
C.L. Cepko
Keyword(s):  
Cortical Neurons ◽  
Intermediate Zone ◽  
Cellular Migration ◽  
Three Dimensions ◽  
Cortical Plate ◽  
Cortical Cells ◽  
Migration Patterns ◽  
Embryonic Mouse ◽  
Radial Migration ◽  
Almost All

The migration patterns of embryonic mouse cortical cells were investigated using a replication-incompetent retrovirus vector (BAG). The lateral ventricles of embryonic day 12 mouse embryos were infected with BAG and brains were harvested 2, 3, 4 and 6 days after infection. The location and morphology of all infected cortical cells were recorded from serial sections of entire brains, which were then reconstructed in three dimensions. Examination of the distribution of labelled cells revealed that there were migration patterns characteristic of each medial-lateral domain of the cortex. In the medial and dorsal areas, migration was often radial, although tangential spread increased with survival time, in large part due to ramification of cells in the intermediate zone. In the dorsolateral and lateral areas of the cortex, radial migration was generally not observed. Rather, variable extents of tangential migration occurred, and often resulted in wide separation of cells in the cortical plate. Almost all of the cellular dispersion occurred in the intermediate zone, although a modest degree of dispersion also occurred within the cortical plate itself. Most dispersion occurred in the mediolateral plane, with relatively little dispersion along the anteroposterior axis. Though characteristic migration patterns could be defined, wide variability in the extents of radial migration and tangential separation of cells was seen. The patterns of migration paralleled the distribution of radial glial fibers in all areas, and are most likely a reflection of the role of this network in supporting the migration of cortical neurons. The extent and variability of cellular dispersion supports a lineage-independent mechanism of cortical column ontogenesis.

Fibroblast growth factor 2 induces apoptosis in the early primary culture of rat cortical neurons

2010 ◽  
Vol 316 (14) ◽  
pp. 2278-2290 ◽  
Author(s):  
Tatsurou Yagami ◽  
Kenkichi Takase ◽  
Yasuhiro Yamamoto ◽  
Keiichi Ueda ◽  
Nobuo Takasu ◽  
...  
Keyword(s):  
Growth Factor ◽  
Primary Culture ◽  
Fibroblast Growth Factor ◽  
Cortical Neurons ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Rat Cortical Neurons ◽  
Early Primary
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