scholarly journals Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos

10.3791/1924 ◽  
2010 ◽  
Author(s):  
Jian Zou ◽  
Xiangyun Wei
Keyword(s):  
Brain Development ◽  
Live Imaging ◽  
Zebrafish Embryos

scholarly journals Ultraspecific live imaging of the dynamics of zebrafish neutrophil granules by a histopermeable fluorogenic benzochalcone probe

2019 ◽  
Vol 10 (12) ◽  
pp. 3654-3670 ◽  
Author(s):  
Emma Colucci-Guyon ◽  
Ariane S. Batista ◽  
Suellen D. S. Oliveira ◽  
Magali Blaud ◽  
Ismael C. Bellettini ◽  
...  
Keyword(s):  
Live Imaging ◽  
Zebrafish Embryos ◽  
Wild Type

A fluorogenic benzochalcone specifically labels live neutrophil granules in whole wild-type, GFP- or RFP-expressing zebrafish embryos and larvae.

Application of in utero electroporation and live imaging in the analyses of neuronal migration during mouse brain development

2012 ◽  
Vol 45 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Yoshiaki V. Nishimura ◽  
Tomoyasu Shinoda ◽  
Yutaka Inaguma ◽  
Hidenori Ito ◽  
Koh-ichi Nagata
Keyword(s):  
Brain Development ◽  
Mouse Brain ◽  
Neuronal Migration ◽  
In Utero ◽  
Live Imaging ◽  
In Utero Electroporation ◽  
Mouse Brain Development

scholarly journals TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella

eLife ◽  
2014 ◽  
Vol 3 ◽  
Author(s):  
Hiroaki Ishikawa ◽  
Takahiro Ide ◽  
Toshiki Yagi ◽  
Xue Jiang ◽  
Masafumi Hirono ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Mammalian Cells ◽  
Intraflagellar Transport ◽  
Live Imaging ◽  
Transport Protein ◽  
Zebrafish Embryos ◽  
Particle Assembly ◽  
Biochemical Analyses ◽  
Related Proteins

Cilia/flagella are assembled and maintained by the process of intraflagellar transport (IFT), a highly conserved mechanism involving more than 20 IFT proteins. However, the functions of individual IFT proteins are mostly unclear. To help address this issue, we focused on a putative IFT protein TTC26/DYF13. Using live imaging and biochemical approaches we show that TTC26/DYF13 is an IFT complex B protein in mammalian cells and Chlamydomonas reinhardtii. Knockdown of TTC26/DYF13 in zebrafish embryos or mutation of TTC26/DYF13 in C. reinhardtii, produced short cilia with abnormal motility. Surprisingly, IFT particle assembly and speed were normal in dyf13 mutant flagella, unlike in other IFT complex B mutants. Proteomic and biochemical analyses indicated a particular set of proteins involved in motility was specifically depleted in the dyf13 mutant. These results support the concept that different IFT proteins are responsible for different cargo subsets, providing a possible explanation for the complexity of the IFT machinery.

scholarly journals Bioorthogonal mRNA labeling at the poly(A) tail for imaging localization and dynamics in live zebrafish embryos

2020 ◽  
Vol 11 (11) ◽  
pp. 3089-3095 ◽  
Author(s):  
Kim J. Westerich ◽  
Karthik S. Chandrasekaran ◽  
Theresa Gross-Thebing ◽  
Nadine Kueck ◽  
Erez Raz ◽  
...  
Keyword(s):  
Live Imaging ◽  
Zebrafish Embryos ◽  
In Cells

Live imaging of mRNA in cells and organisms is important for understanding the dynamic aspects underlying its function.

Functions of thioredoxin1 in brain development and in response to environmental chemicals in zebrafish embryos

2019 ◽  
Vol 314 ◽  
pp. 43-52 ◽  
Author(s):  
Lixin Yang ◽  
Chen Zeng ◽  
Yuanyuan Zhang ◽  
Feifei Wang ◽  
Masanari Takamiya ◽  
...  
Keyword(s):  
Brain Development ◽  
Environmental Chemicals ◽  
Zebrafish Embryos

scholarly journals The second pharyngeal pouch is generated by dynamic remodeling of endodermal epithelium in zebrafish

Development ◽  
2020 ◽  
Vol 147 (24) ◽  
pp. dev194738
Author(s):  
Kazunori Okada ◽  
Shinji Takada
Keyword(s):  
Retinoic Acid ◽  
Live Imaging ◽  
Zebrafish Embryos ◽  
Retinoic Acid Signaling ◽  
Pharyngeal Pouch ◽  
Pharyngeal Arches ◽  
Pharyngeal Endoderm ◽  
Cell Tracing ◽  
The Future ◽  
Epithelial Remodeling

ABSTRACTPharyngeal arches (PAs) are segmented by endodermal outpocketings called pharyngeal pouches (PPs). Anterior and posterior PAs appear to be generated by different mechanisms, but it is unclear how the anterior and posterior PAs combine. Here, we addressed this issue with precise live imaging of PP development and cell tracing of pharyngeal endoderm in zebrafish embryos. We found that two endodermal bulges are initially generated in the future second PP (PP2) region, which separates anterior and posterior PAs. Subsequently, epithelial remodeling causes contact between these two bulges, resulting in the formation of mature PP2 with a bilayered morphology. The rostral and caudal bulges develop into the operculum and gill, respectively. Development of the caudal PP2 and more posterior PPs is affected by impaired retinoic acid signaling or pax1a/b dysfunction, suggesting that the rostral front of posterior PA development corresponds to the caudal PP2. Our study clarifies an aspect of PA development that is essential for generation of a seamless array of PAs in zebrafish.

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