scholarly journals Microvolume Protein Concentration Determination Using the NanoDrop 2000c Spectrophotometer

10.3791/1610 ◽  
2009 ◽  
Author(s):  
Philippe Desjardins ◽  
Joel B. Hansen ◽  
Michael Allen
Keyword(s):  
Protein Concentration ◽  
Concentration Determination

FPCount protocol - Full protocol v1

2021 ◽  
Author(s):  
Eszter Csibra ◽  
Guy-Bart Stan
Keyword(s):  
Protein Concentration ◽  
Fluorescent Protein ◽  
R Package ◽  
Protein Storage ◽  
Concentration Determination ◽  
Plate Reader ◽  
Full Protocol ◽  
Microplate Reader

FPCount is a complete protocol for fluorescent protein calibration, consisting of: 1. FP expression/purification using Thermo's HisPur Cobalt Resin. 2. FP concentration determination in a microplate reader. 3. FP fluorescence quantification in a microplate reader. Results can be analysed with the corresponding R package, FPCountR. --- Summary 1. Expression 2. Harvesting/Washing 3. Lysis 4. Fractionation 5. Gel1: Verification of Expression/Fractions 6. Purification 7. Gel2: Verification of Purification 8. Protein concentration and buffer exchange 9. Quantification of FP concentration (part1) 10. Quantification of FP fluorescence 11. Quantification of FP concentration (part2) 12. Protein Storage 13. Calibration of Plate Reader

Research highlights: nanopore protein detection and analysis

Lab on a Chip ◽  
2015 ◽  
Vol 15 (17) ◽  
pp. 3424-3427 ◽  
Author(s):  
Shiv Acharya ◽  
Shayson Edwards ◽  
Jacob Schmidt
Keyword(s):  
Protein Folding ◽  
Protein Concentration ◽  
Protein Detection ◽  
Specific Protein ◽  
Protein Species ◽  
Concentration Determination

Nanopore detection of specific protein species using carrier DNA – Quantitative protein concentration determination using aptamers – Protein folding studied with unfoldase-coupled nanopores.

FPCount protocol - in-lysate (purification free) protocol v1

2021 ◽  
Author(s):  
Eszter Csibra ◽  
Guy-Bart Stan
Keyword(s):  
Protein Concentration ◽  
Fluorescent Protein ◽  
R Package ◽  
Protein Quantification ◽  
Protein Storage ◽  
Cell Lysates ◽  
Concentration Determination ◽  
His Tag ◽  
Plate Reader ◽  
Microplate Reader

FPCount is a complete protocol for fluorescent protein calibration, consisting of: 1. FP expression and production of cell lysates. 2. FP concentration determination in a microplate reader. 3. FP fluorescence quantification in a microplate reader. Results can be analysed with the corresponding R package, FPCountR. This in-lysate version of the protocol uses the ECmax protein quantification protocol of FPs in lysates and does not require His-tag purification of the FPs. Note that it is only suitable for FPs with entries in FPbase. If you want to verify or validate results, it's recommended you follow the 'short' protocol, which requires FP purification, or the 'complete' protocol, which requires FP purification and compares three protein quantification methods. --- Summary 1. Expression 2. Harvesting/Washing 3. Lysis 4. Fractionation 8. Protein concentration and buffer exchange 9. Quantification of FP concentration (part1) 10. Quantification of FP fluorescence 12. Protein storage 13. Calibration of Plate Reader

Good Old-Fashioned Protein Concentration Determination by

2021 ◽  
pp. 21-28
Author(s):  
Caroline May ◽  
Bettina Serschnitzki ◽  
Katrin Marcus
Keyword(s):  
Protein Concentration ◽  
Concentration Determination
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