scholarly journals Recordings of Neural Circuit Activation in Freely Behaving Animals

10.3791/1297 ◽  
2009 ◽  
Author(s):  
Jens Herberholz
Keyword(s):  
Neural Circuit ◽  
Freely Behaving

scholarly journals Wireless Neural Stimulation in Freely Behaving Small Animals

2009 ◽  
Vol 102 (1) ◽  
pp. 598-605 ◽  
Author(s):  
Scott K. Arfin ◽  
Michael A. Long ◽  
Michale S. Fee ◽  
Rahul Sarpeshkar
Keyword(s):  
Neural Circuit ◽  
Neural Stimulation ◽  
Strong Increase ◽  
Battery Life ◽  
Sleep Mode ◽  
Motor Nucleus ◽  
Wireless Receiver ◽  
Bipolar Electrodes ◽  
Downstream Target ◽  
Freely Behaving

We introduce a novel wireless, low-power neural stimulation system for use in freely behaving animals. The system consists of an external transmitter and a miniature, implantable wireless receiver–stimulator. The implant uses a custom integrated chip to deliver biphasic current pulses to four addressable bipolar electrodes at 32 selectable current levels (10 μA to 1 mA). To achieve maximal battery life, the chip enters a sleep mode when not needed and can be awakened remotely when required. To test our device, we implanted bipolar stimulating electrodes into the songbird motor nucleus HVC (formerly called the high vocal center) of zebra finches. Single-neuron recordings revealed that wireless stimulation of HVC led to a strong increase of spiking activity in its downstream target, the robust nucleus of the arcopallium. When we used this device to deliver biphasic pulses of current randomly during singing, singing activity was prematurely terminated in all birds tested. Thus our device is highly effective for remotely modulating a neural circuit and its corresponding behavior in an untethered, freely behaving animal.

scholarly journals Neural circuit activity in freely behaving zebrafish (Danio rerio)

2011 ◽  
Vol 214 (6) ◽  
pp. 1028-1038 ◽  
Author(s):  
F. A. Issa ◽  
G. O'Brien ◽  
P. Kettunen ◽  
A. Sagasti ◽  
D. L. Glanzman ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Neural Circuit ◽  
Freely Behaving

scholarly journals Targeted manipulation of neuronal activity in behaving adult flies

2016 ◽  
Author(s):  
Stefanie Hampel ◽  
Andrew Michael Seeds
Keyword(s):  
Drosophila Melanogaster ◽  
Neuronal Activity ◽  
Neural Circuits ◽  
Neural Circuit ◽  
Fruit Fly ◽  
Specific Behavior ◽  
Freely Behaving ◽  
The Rich ◽  
Circuit Function

The ability to control the activity of specific neurons in freely behaving animals provides an effective way to probe the contributions of neural circuits to behavior. Wide interest in studying principles of neural circuit function using the fruit fly Drosophila melanogaster has fueled the construction of an extensive transgenic toolkit for performing such neural manipulations. Here we describe approaches for using these tools to manipulate the activity of specific neurons and assess how those manipulations impact the behavior of flies. We also describe methods for examining connectivity among multiple neurons that together form a neural circuit controlling a specific behavior. This work provides a resource for researchers interested in examining how neurons and neural circuits contribute to the rich repertoire of behaviors performed by flies.

scholarly journals Simultaneous electrophysiological recording and fiber photometry in freely behaving mice

2019 ◽  
Author(s):  
Amisha A Patel ◽  
Niall McAlinden ◽  
Keith Mathieson ◽  
Shuzo Sakata
Keyword(s):  
Neural Circuit ◽  
Electrophysiological Recording ◽  
Field Potentials ◽  
Cell Type ◽  
Specific Population ◽  
Population Activity ◽  
Custom Made ◽  
Cell Type Specific ◽  
Freely Behaving

AbstractIn vivo electrophysiology is the gold standard technique used to investigate sub-second neural dynamics in freely behaving animals. However, monitoring cell-type-specific population activity is not a trivial task. Over the last decade, fiber photometry based on genetically encoded calcium indicators has been widely adopted as a versatile tool to monitor cell-type-specific population activity in vivo. However, this approach suffers from low temporal resolution. Here, we combine these two approaches to monitor both sub-second field potentials and cell-type-specific population activity in freely behaving mice. By developing an economical custom-made system, and constructing a hybrid implant of an electrode and a fiber optic cannula, we simultaneously monitor artifact-free pontine field potentials and calcium transients in cholinergic neurons across the sleep-wake cycle. We find that pontine cholinergic activity co-occurs with sub-second pontine waves, called P-waves, during rapid eye movement sleep. Given the simplicity of our approach, simultaneous electrophysiological recording and cell-type-specific imaging provides a novel and valuable tool for interrogating state-dependent neural circuit dynamics in vivo.

scholarly journals Targeted manipulation of neuronal activity in behaving adult flies

2016 ◽  
Author(s):  
Stefanie Hampel ◽  
Andrew Michael Seeds
Keyword(s):  
Drosophila Melanogaster ◽  
Neuronal Activity ◽  
Neural Circuits ◽  
Neural Circuit ◽  
Fruit Fly ◽  
Specific Behavior ◽  
Freely Behaving ◽  
The Rich ◽  
Circuit Function

The ability to control the activity of specific neurons in freely behaving animals provides an effective way to probe the contributions of neural circuits to behavior. Wide interest in studying principles of neural circuit function using the fruit fly Drosophila melanogaster has fueled the construction of an extensive transgenic toolkit for performing such neural manipulations. Here we describe approaches for using these tools to manipulate the activity of specific neurons and assess how those manipulations impact the behavior of flies. We also describe methods for examining connectivity among multiple neurons that together form a neural circuit controlling a specific behavior. This work provides a resource for researchers interested in examining how neurons and neural circuits contribute to the rich repertoire of behaviors performed by flies.

scholarly journals Endogenous and exogenous control of visuospatial selective attention in freely behaving mice

2019 ◽  
Author(s):  
Wen-Kai You ◽  
Shreesh P. Mysore
Keyword(s):  
Spatial Attention ◽  
Neural Circuit ◽  
Flanker Task ◽  
Visuospatial Attention ◽  
Lower Probability ◽  
Response Accuracy ◽  
Evidence Accumulation ◽  
Spatial Probability ◽  
Probability Task ◽  
Freely Behaving

SUMMARYSelective spatial attention, the ability to dynamically prioritize the most important spatial location, is essential for adaptive behavior. It has been studied primarily in head-fixed animals, and almost exclusively in primates. Here, we report the development of two human-inspired, discrimination-based behavioral paradigms for studying selective visuospatial attention in the freely behaving mouse: the spatial probability task, and the flanker task. In the spatial probability task, we found enhanced response accuracy, perceptual discriminability, and rates of sensory evidence accumulation at the location with higher probability of target occurrence, and opposite effects at the lower probability location. In the absence of systematic differences in sensory input, motor biases, and trial structure, these results demonstrated endogenous expectation-driven shifts of spatial attention. In the flanker task, we found that a second, ‘flanker’ stimulus presented with the target, but with incongruent information, caused switch-like decrements in response accuracy and perceptual discriminability as a function of flanker contrast, as well as a reduced rate of evidence accumulation. These results demonstrated exogenous capture of spatial attention. The innovation of behavioral tasks for selective visuospatial attention in unrestrained mice opens up a rich avenue for future research dissecting the neural circuit mechanisms underlying this critical executive function.

scholarly journals Targeted manipulation of neuronal activity in behaving adult flies

2016 ◽  
Author(s):  
Stefanie Hampel ◽  
Andrew Michael Seeds
Keyword(s):  
Drosophila Melanogaster ◽  
Neuronal Activity ◽  
Neural Circuits ◽  
Neural Circuit ◽  
Fruit Fly ◽  
Specific Behavior ◽  
Freely Behaving ◽  
The Rich ◽  
Circuit Function

The ability to control the activity of specific neurons in freely behaving animals provides an effective way to probe the contributions of neural circuits to behavior. Wide interest in studying principles of neural circuit function using the fruit fly Drosophila melanogaster has fueled the construction of an extensive transgenic toolkit for performing such neural manipulations. Here we describe approaches for using these tools to manipulate the activity of specific neurons and assess how those manipulations impact the behavior of flies. We also describe methods for examining connectivity among multiple neurons that together form a neural circuit controlling a specific behavior. This work provides a resource for researchers interested in examining how neurons and neural circuits contribute to the rich repertoire of behaviors performed by flies.

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