CAENORHABDITIS ELEGANS, GALLERIA MELLONELLA E DROSOPHILA MELANOGASTER COMOMODELOS ALTERNATIVOS PARA PESQUISA IN VIVO DE PLANTAS MEDICINAIS

2021 ◽  
pp. 156-163
Author(s):  
Raquel Borges de Barros Primo ◽  
Jacenir Vieira da Silva ◽  
Larissa P. Mueller ◽  
Flávio H. S. Araújo ◽  
Silvia Aparecida Oesterreich
Keyword(s):  
Drosophila Melanogaster ◽  
Caenorhabditis Elegans ◽  
Galleria Mellonella

scholarly journals Elevated CO2 regulates the Wnt signaling pathway in mammals, Drosophila melanogaster and Caenorhabditis elegans

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Masahiko Shigemura ◽  
Emilia Lecuona ◽  
Martín Angulo ◽  
Laura A. Dada ◽  
Melanie B. Edwards ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Caenorhabditis Elegans ◽  
Wnt Signaling ◽  
Large Scale ◽  
Wnt Pathway ◽  
Wnt Signaling Pathway ◽  
Secondary Analysis ◽  
Evolutionarily Conserved ◽  
Integrated Or

AbstractCarbon dioxide (CO2) is sensed by cells and can trigger signals to modify gene expression in different tissues leading to changes in organismal functions. Despite accumulating evidence that several pathways in various organisms are responsive to CO2 elevation (hypercapnia), it has yet to be elucidated how hypercapnia activates genes and signaling pathways, or whether they interact, are integrated, or are conserved across species. Here, we performed a large-scale transcriptomic study to explore the interaction/integration/conservation of hypercapnia-induced genomic responses in mammals (mice and humans) as well as invertebrates (Caenorhabditis elegans and Drosophila melanogaster). We found that hypercapnia activated genes that regulate Wnt signaling in mouse lungs and skeletal muscles in vivo and in several cell lines of different tissue origin. Hypercapnia-responsive Wnt pathway homologues were similarly observed in secondary analysis of available transcriptomic datasets of hypercapnia in a human bronchial cell line, flies and nematodes. Our data suggest the evolutionarily conserved role of high CO2 in regulating Wnt pathway genes.

scholarly journals HEWAN SEBAGAI MODEL PENYAKIT INFEKSI PERNAFASAN YANG DISEBABKAN OLEH BAKTERI

2021 ◽  
Vol 4 (1) ◽  
pp. 48-56
Author(s):  
Noor Andryan Ilsan ◽  
Siti Nurfajriah ◽  
Maulin Inggraini
Keyword(s):  
Caenorhabditis Elegans ◽  
Danio Rerio ◽  
Galleria Mellonella

Pendahuluan: Penyakit infeksi pernafasan karena bakteri merupakan penyakit yang memiliki kasus tinggi di Indonesia. Penyakit pernafasan karena infeksi bakteri juga bersifat nosokomial dan dapat menyebar di komunitas. Bakteri yang menyebabkan infeksi pernafasan ini sangat beragam baik dari jenis bakterinya, tingkat resistensinya, maupun tingkat virulensinya. Tingkat virulensi bakteri mempengaruhi konsekuensi penyakitnya pada pasien. Metode: Dalam menentukan virulensi bakteri secara in vivo, beberapa hewan dapat digunakan sebagai model infeksi pernafasan karena bakteri seperti tikus, ikan zebra (Danio rerio), ngengat lilin (Galleria mellonella), nematoda Caenorhabditis elegans. Hasil: Dari sudut pandang author, jika menilik biaya dan kemudahan sebagai prioritas, ulat G. mellonella memiliki beberapa keunggulan dibandingkan hewan lain seperti biaya produksi murah, tidak membutuhkan perizinan etik, dapat diinkubasi pada suhu 37° C, juga sudah banyak publikasi yang menggunakan ulat ini dalam uji virulensi bakteri. Kesimpulan: Review artikel ini akan menjelaskan perbandingan kelebihan dan kekurangan hewan model tersebut dalam model in vivo bakteri infeksi pernafasan.

scholarly journals Galleria mellonella insektua eta Caenorhabditis elegans nematodoa, infekzio eredu boteretsuak Candida glabrata eta erlazionatutako espezieen birulentzia ikertzeko

2020 ◽  
pp. 175-190
Author(s):  
Ainara Hernando-Ortiz ◽  
Estibaliz Mateo ◽  
Marcelo Ortega-Riveros ◽  
Iker De-la-Pinta ◽  
Guillermo Quindós ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Caenorhabditis Elegans ◽  
Candida Glabrata ◽  
Galleria Mellonella ◽  
Candida Bracarensis ◽  
Candida Nivariensis

Kandidiasia Candida generoko espezieek eragindako infekzio mikotikoa da. Candida albicans agente etiologiko nagusia da baina gero eta gehiagotan Candida generoko beste espezie batzuk agertzen ari dira kandidiasiaren eragile bezala eta, hauen artean, Candida glabrata espeziea. Espezie honekin lotuta beste bi espezie daude, Candida bracarensis eta Candida nivariensis, teknika molekularrek ondo desberdindu ditzaketenak. Aldaketa etiologiko hauek ondorio larriak izan ditzakete kandidiasiaren diagnostikoan, tratamenduan edota pronostikoan, besteak beste, bere patogenia edo antifungikoekiko sentikortasuna oso desberdina izan daitezkeelako. Ordezko eredu esperimentalek erabiliz mikroorganismoek eragindako gaixotasunen patogenia eta terapia ezagutzeko ezinbesteko aukera ematen dizkigute, eta hauen artean, Caenorhabditis elegans nematodoa eta Galleria mellonella lepidopteroa ditugu. Ikerketa lan honetan konbentzionalak ez diren bi animalia eredu hauen erabilgarritasuna ebaluatu nahi izan da Candida glabrata, Candida bracarensis eta Candida nivariensis harreman filogenetiko estua duten hiru espezie hauen birulentzia in vivo aztertzeko.

Insights into amyloid disease from fly models

2014 ◽  
Vol 56 ◽  
pp. 69-83 ◽  
Author(s):  
Ko-Fan Chen ◽  
Damian C. Crowther
Keyword(s):  
Drosophila Melanogaster ◽  
Neurodegenerative Disorders ◽  
Amyloid Β ◽  
Amyloid Β Peptide ◽  
Disease Pathogenesis ◽  
Amyloid Aggregates ◽  
Aβ Amyloid ◽  
Polypeptide Chains ◽  
Amyloid Diseases

The formation of amyloid aggregates is a feature of most, if not all, polypeptide chains. In vivo modelling of this process has been undertaken in the fruitfly Drosophila melanogaster with remarkable success. Models of both neurological and systemic amyloid diseases have been generated and have informed our understanding of disease pathogenesis in two main ways. First, the toxic amyloid species have been at least partially characterized, for example in the case of the Aβ (amyloid β-peptide) associated with Alzheimer's disease. Secondly, the genetic underpinning of model disease-linked phenotypes has been characterized for a number of neurodegenerative disorders. The current challenge is to integrate our understanding of disease-linked processes in the fly with our growing knowledge of human disease, for the benefit of patients.

INFLUÊNCIA DO SUBSTRATO E DA LUMINOSIDADE NA INFECÇÃO DE NEMATOIDES ENTOMOPATOGÊNICOS EM Galleria mellonella (LEPIDOPTERA: PYRALIDAE)

2018 ◽  
Vol 20 (2) ◽  
pp. 91-101
Author(s):  
Andressa Lima de Brida ◽  
Silvia Renata Siciliano Wilcken ◽  
Luis Garrigós Leite
Keyword(s):  
Galleria Mellonella ◽  
Steinernema Carpocapsae ◽  
Steinernema Feltiae ◽  
Lepidoptera Pyralidae

Nematoides entomopatogênicos (NEPs) são alternativas eficientes para o controle de pragas. O emprego de novas técnicas da produção in vivo, permite o progresso da tecnologia de formulação de bioinseticidas. O objetivo do trabalho, foi avaliar a influência da luminosidade e do substrato na capacidade de infecção de juvenis infectantes (JIs) de Steinernema brazilense IBCBn 06, Steinernema carpocapsae IBCBn 02, Steinernema feltiae IBCBn 47 e Heterorhabditis amazonensis IBCBn 24 em lagartas de Galleria mellonella (Lepidoptera: Pyralidae). O delineamento experimental foi inteiramente casualizado com quatro tratamentos e oito repetições. As parcelas, constituídas por placa de Petri com, substrato-areia e substrato-papel filtro, com e sem luminosidade, inoculados com suspensão de 1,5 mL contendo 400JIs e quatro lagartas de G. mellonella. O número de JIs foi quantificado após a mortalidade das lagartas. A taxa de infecção de JIs de S. carpocapsae IBCBn 02 e S. feltiae IBCBn 47 variaram de 2,14 a 3,28 e de 11,04 a 13,09 JIs/lagarta. O substrato-areia com e sem luminosidade permitiu a maior taxa de infeção dos JIs de S. brazilense IBCBn 06 de 7,86 e 9,44 JIs/lagarta, e 13,49 JIs/lagarta com luminosidade para H. amazonensis IBCBn 24. O substrato-areia, permite a maior taxa de infecção por JIs de NEPs.

Sign in / Sign up

Export Citation Format

Share Document