POTENCIAL DE MAURITIA FLEXUOSA MART. COMO ANTIMICROBIANO E MODULADOR DE RESISTÊNCIA DE CEPAS DE STAPHYLOCOCCUS AUREUS

Modulation of the Antibiotic Activity by the Mauritia flexuosa (Buriti) Fixed Oil against Methicillin-Resistant Staphylococcus Aureus (MRSA) and Other Multidrug-Resistant (MDR) Bacterial Strains

Pathogens ◽

10.3390/pathogens7040098 ◽

2018 ◽

Vol 7 (4) ◽

pp. 98 ◽

Cited By ~ 6

Author(s):

Yara Faustino Pereira ◽

Maria do Socorro Costa ◽

Saulo Relison Tintino ◽

Janaína Esmeraldo Rocha ◽

Fábio Fernandes Galvão Rodrigues ◽

...

Keyword(s):

Fatty Acids ◽

Staphylococcus Aureus ◽

Oleic Acid ◽

Unsaturated Fatty Acids ◽

Saturated Fatty Acids ◽

Physicochemical Characterization ◽

Composition Analysis ◽

Palm Tree ◽

Bacterial Strains ◽

Mauritia Flexuosa

Mauritia flexuosa (buriti) is a typical Brazilian palm tree found in swampy regions with many plant forms. The fruit has various purposes with the pulps to the seeds being used for ice creams, sweets, creams, jellies, liqueurs, and vitamin production. A physicochemical characterization of the fixed pulp oil and its antibacterial and aminoglycoside antibiotic modifying activity against Gram-positive and Gram-negative multiresistant bacterial strains were performed using broth microdilution assays. Physical properties, such as moisture, pH, acidity, peroxide index, relative density, and refractive index, indicated oil stability and chemical quality. In the GC/MS chemical composition analysis, a high content of unsaturated fatty acids (89.81%) in relation to saturated fatty acids (10.19%) was observed. Oleic acid (89.81%) was the main fatty acid identified. In the antibacterial test, the fixed oil obtained the Minimum Inhibitory Concentration (MIC) ≥ 1024 μg/mL for all standard and multiresistant bacterial strains. The synergic effect of fixed pulp oil combined was observed only in Staphylococcus aureus SA–10, with an MIC reduction of the gentamicin and amikacin by 40.00% and 60.55%, respectively. The data indicates the M. flexuosa fixed oil as a valuable source of oleic acid and modulator of aminoglycoside activity.

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Evaluation of the Antimicrobial Activity of Buriti (Mauritia Flexuosa) Pulp Extracts

The Open Food Science Journal ◽

10.2174/1874256401911010089 ◽

2019 ◽

Vol 11 (1) ◽

pp. 89-94

Author(s):

Carla Roana Moraes Monteiro ◽

Adriele Rodrigues dos Santos ◽

Marcos Vieira da Silva ◽

Adenilson Renato Rudke ◽

Iara de Melo Rodrigues ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Inhibitory Concentration ◽

Minimum Bactericidal Concentration ◽

Palm Tree ◽

Gram Negative ◽

Mauritia Flexuosa ◽

Pharmaceutical Industries ◽

Positive Results

Background: Buriti (Mauritia flexuosa) is a palm tree typical of the Amazon region. This plant belongs to the Arecaceae family and is economically important because it contains substances important for the food, cosmetic and pharmaceutical industries. It has, in its fruits, compounds with antimicrobial potential. Objective: The objective of this study was to evaluate the minimal inhibitory concentration and minimum bactericidal concentration of the Buriti extracts against the four bacteria: Salmonella enterica serotype Typhimurium ATCC 14028, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853. Methods: 15 extracts from buriti pulp were obtained varying in temperature, mass of buriti and ethanol content. The antimicrobial activity of these extracts was evaluated. To determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), the methodology recommended by the Clinical and Laboratory Standards Institute was followed. Results: The results showed which buriti pulp extracts had strong inhibitory activity. Gram-positive results ranged from 21 to 78 μg.mL-1. For Gram-negative, they ranged from 30 to 111 μg.mL-1. Conclusion: The buriti extracts significantly slowed the growth of the tested bacteria.

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Antibacterial effect of the hydroalcoholic extract of Mauritia flexuosa leaves on gram-negative and gram-positive bacteria

F1000Research ◽

10.12688/f1000research.19151.1 ◽

2019 ◽

Vol 8 ◽

pp. 1487

Author(s):

Ana Sandoval Vergara ◽

Marlon Farfán Córdova ◽

Marco Leoncio Salazar Castillo ◽

Icela Marissa Rodríguez Haro ◽

Ana Paula Vizconde Rodríguez

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Effect ◽

Gram Negative Bacteria ◽

Gram Positive ◽

Hydroalcoholic Extract ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Extract Concentration ◽

Mauritia Flexuosa

Background: Plant-derived compounds are sometimes used as substitutes for pharmaceuticals. Mauritia flexuosa is a palm tree that is widely distributed in South America, especially in the Amazon region. The San Martín region of Peru, in which this species of the Arecaceae family is found, has great biological diversity and there is economic potential in the utilization of natural resources in the region. Methods: In this study, the antibacterial effect of the hydroalcoholic extract of Mauritia flexuosa leaves was evaluated for gram-positive bacteria Staphylococcus aureus ATCC 25923 and Bacillus subtilis ATCC 6633 and gram-negative Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Salmonella enterica subsp. enterica ser. Typhi ATCC 11011. Mauritia flexuosa leaves were used to prepare concentrations of 10, 20, 40 and 60mg/ml. Phytochemical analysis was performed to identify secondary metabolites in the plants. For the experiment, 10 Mueller-Hinton agar plates were prepared and 1ml of bacterial inoculum, standardized to 0.5 McFarland, was added to each plate. The hydroalcoholic extract was added via the diffusion method, making five holes of 5mm each (four with extract concentrations and one with distilled water as a control group), and the plates were incubated for 24 hours at 36°C. The inhibition halo was measured in mm using a digital vernier caliper. Results: For gram-negative bacteria, an antibacterial effect was demonstrated for Pseudomonas aeruginosa only, at an extract concentration of 60mg/ml, with an inhibition halo of 14.8 mm. For gram-positive bacteria Baccillus subtilis and Staphylococcus aureus, an antibacterial effect was demonstrated at an extract concentration of 60mg/ml, with inhibition halos of 13.2mm and 15.4mm in diameter, respectively. Conclusion: It can be concluded that the hydroalcoholic extract of Mauritia flexuosa does not inhibit bacterial growth for gram-negative bacteria Salmonella Typhi and Escherichia coli.

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Cytochemical Studies of Cell Wall Biosynthesis in Staphylococcus Aureus

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100094905 ◽

1972 ◽

Vol 30 ◽

pp. 328-329

Author(s):

Masaatsu Koike ◽

Koichi Nakashima ◽

Kyoko Iida

Keyword(s):

Staphylococcus Aureus ◽

Periodate Oxidation ◽

Early Time ◽

The Other ◽

Penicillin G ◽

Cell Wall Biosynthesis ◽

Septal Wall ◽

Peptidoglycan Biosynthesis ◽

Gram Positive Bacteria ◽

Cross Linkage

Penicillin exerts the activity to inhibit the peptide cross linkage between each polysaccharide backbone at the final stage of wall-peptidoglycan biosynthesis of bacteria. Morphologically, alterations of the septal wall and mesosome in gram-positive bacteria, which were occurred in early time after treatment with penicillin, have been observed. In this experiment, these alterations were cytochemically investigated by means of silver-methenamine staining after periodate oxidation, which is applied for detection of localization of wall mucopolysaccharide.Staphylococcus aureus strain 209P treated with 100 u/ml of penicillin G was divided into two aliquotes. One was fixed by Kellenberger-Ryter's OSO4 fixative at 30, 60 and 120 min after addition of the antibiotic, dehydrated through alcohol series, and embedded in Epon 812 (Specimen A). The other was fixed by 21 glutaraldehyde, dehydrated through glycolmethacrylate series and embedded in glycolmethacrylate mixture, according to Bernhard's method (Specimen B).

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Gold labeling of teichoic acid on adjacent surfaces of staphylococcus aureus

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100160443 ◽

1990 ◽

Vol 48 (3) ◽

pp. 578-579

Author(s):

Margaret Hukee

Keyword(s):

Staphylococcus Aureus ◽

Teichoic Acid ◽

Protein Labeling ◽

Minimal Amount ◽

Section Thickness ◽

Double Labeling ◽

Parallel Surfaces ◽

Supporting Membrane

Gold labeling of two antigens (double labeling) is often done on two section surfaces separated by section thickness. Whether labeling is done on both sides of the same section or on two parallel surfaces separated by section thickness (PSSST), comparable results are dependent on an equal number of epitopes being exposed at each surface. We propose a method to study protein labeling within the same field of proteins, by examining two directly adjacent surfaces that were split during sectioning. The number of labeling sites on adjacent surfaces (AS) were compared to sites on PSSST surfaces in individual bacteria.Since each bacteria needed to be recognizable in all three section surfaces, one-hole grids were used for labeling. One-hole grids require a supporting membrane and excessive handling during labeling often ruptures the membrane. To minimize handling, a labeling chamber was designed that is inexpensive, disposable, minimizes contamination, and uses a minimal amount of solution.

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