High-speed 3D imaging based on structured illumination and electrically tunable lens

2017 ◽  
Vol 15 (9) ◽  
pp. 090004 ◽  
Author(s):  
Dongping Wang Dongping Wang ◽  
Yunlong Meng Yunlong Meng ◽  
Dihan Chen Dihan Chen ◽  
Yeung Yam Yeung Yam ◽  
Shih-Chi Chen Shih-Chi Chen
Keyword(s):  
High Speed ◽  
3D Imaging ◽  
Structured Illumination ◽  
Tunable Lens

scholarly journals The human sperm beats anisotropically and asymmetrically in 3D

2019 ◽  
Author(s):  
Hermes Gadêlha ◽  
Paul Hernández-Herrera ◽  
Fernando Montoya ◽  
Alberto Darszon ◽  
Gabriel Corkidi
Keyword(s):  
Ion Channels ◽  
Mathematical Analysis ◽  
Molecular Motors ◽  
High Speed ◽  
3D Imaging ◽  
Human Sperm ◽  
Travelling Wave ◽  
Fundamental Question ◽  
Sperm Flagellum ◽  
Straight Line

The canonical beating of the human sperm flagellum is postulated to be symmetric. This is despite the reported asymmetries inherent to the flagellar axonemal structure, from distribution and activation of molecular motors to, even, the localisation of regulatory ion channels. This raises a fundamental question: how symmetric beating is possible within such intrinsically asymmetric flagellar complex? Here, we employ high-speed 3D imaging with mathematical analysis capable of resolving the flagellar movement in 4D (3D+time). This reveals that the human sperm beating is both anisotropic and asymmetric, and composed by a superposition of two transversal waves: an asymmetric travelling wave and a symmetric standing wave. This novel anisotropic travelling-pulsation mechanism induces sperm rolling self-organisation and causes a flagellar kinematic illusion, so that the beat appears to be symmetric if observed with 2D microscopy. The 3D beating anisotropy thus regularises the intrinsic flagellar asymmetry to achieve symmetric side-to-side movement and straight-line swimming.

scholarly journals High-speed dual-layer scanning photoacoustic microscopy using focus tunable lens modulation at resonant frequency

2017 ◽  
Vol 25 (22) ◽  
pp. 26427 ◽  
Author(s):  
Kiri Lee ◽  
Euiheon Chung ◽  
Seungrag Lee ◽  
Tae Joong Eom
Keyword(s):  
Resonant Frequency ◽  
High Speed ◽  
Photoacoustic Microscopy ◽  
Tunable Lens

High-Speed Structured Illumination Microscopy and Its Applications

2020 ◽  
Vol 57 (24) ◽  
pp. 240001
Author(s):  
赵天宇 Zhao Tianyu ◽  
汪召军 Wang Zhaojun ◽  
冯坤 Feng Kun ◽  
梁言生 Liang Yansheng ◽  
何旻儒 He Minru ◽  
...  
Keyword(s):  
High Speed ◽  
Structured Illumination ◽  
Structured Illumination Microscopy

Fast 3D imaging algorithm for high-speed spinning targets based on GRT-CLEAN

2018 ◽  
Vol 33 (3) ◽  
pp. 228-238
Author(s):  
王 会 WANG Hui ◽  
巨 欢 JU Huan ◽  
方 阳 FANG Yang ◽  
李荣旭 LI Rong-xu ◽  
王保平 WANG Bao-ping
Keyword(s):  
High Speed ◽  
3D Imaging ◽  
Imaging Algorithm ◽  
Spinning Targets

High-speed 3D imaging of the beating heart using temporal estimation

1987 ◽  
Vol 39 (3) ◽  
pp. 279-290 ◽  
Author(s):  
R.S. Acharya ◽  
P.B. Heffernan ◽  
R.A. Robb ◽  
H. Wechsler
Keyword(s):  
High Speed ◽  
3D Imaging ◽  
Beating Heart ◽  
Temporal Estimation

scholarly journals High-speed Imaging at 3 Tesla: A Technical and Clinical Review with an Emphasis on Whole-brain 3D Imaging

2004 ◽  
Vol 3 (4) ◽  
pp. 177-187 ◽  
Author(s):  
Shinji NAGANAWA ◽  
Hisashi KAWAI ◽  
Hiroshi FUKATSU ◽  
Takeo ISHIGAKI ◽  
Tomomi KOMADA ◽  
...  
Keyword(s):  
Clinical Review ◽  
High Speed ◽  
3D Imaging ◽  
3 Tesla ◽  
High Speed Imaging ◽  
Whole Brain

scholarly journals High-speed multiplane structured illumination microscopy of living cells using an image-splitting prism

Nanophotonics ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 143-148
Author(s):  
Adrien Descloux ◽  
Marcel Müller ◽  
Vytautas Navikas ◽  
Andreas Markwirth ◽  
Robin van den Eynde ◽  
...  
Keyword(s):  
High Speed ◽  
Three Dimensional ◽  
Super Resolution ◽  
Optical Element ◽  
Spatial Light Modulators ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Image Stack ◽  
Light Modulators ◽  
3D Information

AbstractSuper-resolution structured illumination microscopy (SR-SIM) can be conducted at video-rate acquisition speeds when combined with high-speed spatial light modulators and sCMOS cameras, rendering it particularly suitable for live-cell imaging. If, however, three-dimensional (3D) information is desired, the sequential acquisition of vertical image stacks employed by current setups significantly slows down the acquisition process. In this work, we present a multiplane approach to SR-SIM that overcomes this slowdown via the simultaneous acquisition of multiple object planes, employing a recently introduced multiplane image splitting prism combined with high-speed SIM illumination. This strategy requires only the introduction of a single optical element and the addition of a second camera to acquire a laterally highly resolved 3D image stack. We demonstrate the performance of multiplane SIM by applying this instrument to imaging the dynamics of mitochondria in living COS-7 cells.

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