Early-photon guided reconstruction method for time-domain fluorescence lifetime tomography

2016 ◽  
Vol 14 (7) ◽  
pp. 071702-71706 ◽  
Author(s):  
Lin Zhang Lin Zhang ◽  
Chuangjian Cai Chuangjian Cai ◽  
Yanlu Lv Yanlu Lv ◽  
and Jianwen Luo and Jianwen Luo
Keyword(s):  
Fluorescence Lifetime ◽  
Time Domain ◽  
Reconstruction Method

scholarly journals In Vivo Time Domain Optical Imaging of Renal Ischemia-Reperfusion Injury: Discrimination Based on Fluorescence Lifetime

2007 ◽  
Vol 6 (5) ◽  
pp. 7290.2007.00030 ◽  
Author(s):  
Abedelnasser Abulrob ◽  
Eric Brunette ◽  
Jacqueline Slinn ◽  
Ewa Baumann ◽  
Danica Stanimirovic
Keyword(s):  
Optical Imaging ◽  
Fluorescence Lifetime ◽  
Fluorescence Intensity ◽  
Time Domain ◽  
Ex Vivo ◽  
Renal Ischemia ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Lifetime Analysis

Fluorescence lifetime is an intrinsic parameter of the fluorescent probe, independent of the probe concentration but sensitive to changes in the surrounding microenvironment. Therefore, fluorescence lifetime imaging could potentially be applied to in vivo diagnostic assessment of changes in the tissue microenvironment caused by disease, such as ischemia. The aim of this study was to evaluate the utility of noninvasive fluorescence lifetime imaging in distinguishing between normal and ischemic kidney tissue in vivo. Mice were subjected to 60-minute unilateral kidney ischemia followed by 6-hour reperfusion. Animals were then injected with the near-infrared fluorescence probe Cy5.5 or saline and imaged using a time-domain small-animal optical imaging system. Both fluorescence intensity and lifetime were acquired. The fluorescence intensity of Cy5.5 was clearly reduced in the ischemic compared with the contralateral kidney, and the fluorescence lifetime of Cy5.5 was not detected in the ischemic kidney, suggesting reduced kidney clearance. Interestingly, the two-component lifetime analysis of endogenous fluorescence at 700 nm distinguished renal ischemia in vivo without the need for Cy5.5 injection for contrast enhancement. The average fluorescence lifetime of endogenous tissue fluorophores was a sensitive indicator of kidney ischemia ex vivo. The study suggests that fluorescence lifetime analysis of endogenous tissue fluorophores could be used to discriminate ischemic or necrotic tissues by noninvasive in vivo or ex vivo organ imaging.

Reconstruction From Antenna-Transformed Radar Data Using a Time-Domain Reconstruction Method

2007 ◽  
Vol 45 (3) ◽  
pp. 689-696 ◽  
Author(s):  
Hui Zhou ◽  
Motoyuki Sato ◽  
Takashi Takenaka ◽  
Guofa Li
Keyword(s):  
Time Domain ◽  
Radar Data ◽  
Reconstruction Method

A wide-field time-domain fluorescence lifetime imaging microscope with optical sectioning

2002 ◽  
Vol 73 (4) ◽  
pp. 1898-1907 ◽  
Author(s):  
S. E. D. Webb ◽  
Y. Gu ◽  
S. Lévêque-Fort ◽  
J. Siegel ◽  
M. J. Cole ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Time Domain ◽  
Wide Field ◽  
Fluorescence Lifetime Imaging ◽  
Optical Sectioning ◽  
Lifetime Imaging

scholarly journals Data Processing for Time-Domain Fluorescence Lifetime Imaging Microscopy

2013 ◽  
Vol 19 (S2) ◽  
pp. 758-759
Author(s):  
P.A. Young ◽  
A. Grislis ◽  
P.R. Barber ◽  
P.J. Keely ◽  
K.W. Eliceiri
Keyword(s):  
Data Processing ◽  
Fluorescence Lifetime ◽  
Time Domain ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

Extended abstract of a paper presented at Microscopy and Microanalysis 2013 in Indianapolis, Indiana, USA, August 4 – August 8, 2013.

Detection Method of Dissolved Oxygen Concentration in Water Based on Time-Domain Fluorescence Lifetime

2018 ◽  
Vol 38 (10) ◽  
pp. 1001005 ◽  
Author(s):  
涂梦迪 Tu Mengdi ◽  
殷高方 Yin Gaofang ◽  
赵南京 Zhao Nanjing ◽  
覃志松 Qin Zhisong ◽  
王翔 Wang Xiang ◽  
...  
Keyword(s):  
Dissolved Oxygen ◽  
Oxygen Concentration ◽  
Fluorescence Lifetime ◽  
Time Domain ◽  
Detection Method ◽  
Dissolved Oxygen Concentration ◽  
Water Based
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