scholarly journals Microbiological properties of root zone of coconut grown in different soil series in Sri Lanka

CORD ◽  
2005 ◽  
Vol 21 (01) ◽  
pp. 34
Author(s):  
K.S.K.S. Fernando ◽  
N.A. Tennakoon ◽  
S. Widanapathirana

In Sri Lanka, coconut is cultivated under diverse soil conditions of varying fertility status available in different agro-climatic zones. Although chemical and physical characteristics in some of these soils are known, their biological properties are yet to be studied.  Hence the aim of this study was to determine the microbiological parameters in root zone of coconut grown in different soil series.  For this study, 20 different soil series in coconut growing area were analyzed for number of total bacterial and fungal colonies, identification of bacteria and fungi, biomass C and N; CO2 evolution and nitrogen mineralization.   The microbiological parameters varied with the soil series. The bacterial population densities were significantly (p=0.001) high in Ambakelle, Kalpitiya, Rathupasa and Madampe soil series while the fungal colonies were significantly (p=0.001) high in Thambarawa, Pallama, Wilatawa and Ambakelle soil series.   Only Kalpitiya, Kuliyapitiya, Gambura soil series contained biomass C more than 200 mg/100 g of soils.  Biomass N was the highest in Boralu soil series followed by Kalpitiya, Rathupasa and Gambura.  Carbon diozide evolution was high in Kalpitiya, Katunayaka, Wariyapola soil series while N mineralization rate was high in Ambakelle, Weliketiya and Rathupasa soil series.  Bacillus spp. were the dominant bacteria in all studied soil series.  In addition, Micrococcus, Staphylococcus, Enterobacter, Serratia, Corynebacterium, Pseudomonas and Actinomycetes were also identified. Fungi such as Penicillium, Aspergillus and Curvularia were common in most of the soil series than other species.

CORD ◽  
2004 ◽  
Vol 20 (01) ◽  
pp. 34
Author(s):  
K.S.K.S. Fernando ◽  
N.A. Tennakoon ◽  
S. Widanapathirana

In Sri Lanka, coconut is cultivated under diverse soil conditions of varying fertility status available in different agro-climatic zones. Although chemical and physical characteristics in some of these soils are known, their biological properties are yet to be studied.   Hence the aim of this study was to determine the microbiological parameters in root zone of coconut grown in different soil series.  For this study, 20 different soil series in coconut growing area were analyzed for number of total bacterial and fungal colonies, identification of bacteria and fungi, biomass C and N; CO2 evolution and nitrogen mineralization.   The microbiological parameters varied with the soil series. The bacterial population densities   were   significantly   (p=0.001)   high   in  Ambakelle,   Kalpitiya,   Rathupasa   and Madampe soil series while the fungal colonies were significantly (p=0.001) high in Thambarawa, Pallama, Wilatawa and Ambakelle soil series.


2021 ◽  
Vol 16 (1) ◽  
pp. 34-45
Author(s):  
Nur Baitul Izati Rasli ◽  
Nor Azam Ramli ◽  
Mohd Rodzi Ismail

Occupants of buildings spent most of their time indoors and posed themselves to the indoor biological contaminants (i.e. bacteria and fungi) in the built environment. However, the factors that influenced the prevalence of suspended bacteria and fungi in the air of the built environment are not well recognised. Authorities must take the growth of biological contaminants seriously to prevent serious health effects among occupants of buildings. This review summarises the factors (i.e. outdoor air, dust, ventilation problems, humidity/moisture surface problems and human occupancy) of indoor biological contaminants and their species in buildings that could worsen the indoor air quality. Bacteria species could exist in various indoor buildings (i.e. schools, cafeterias, mosques, faculties, child day-care centres, hospitals, dwellings, educational buildings, homes, elderly care centres, libraries, restaurants, and factories). The dominant bacteria were Bacillus spp. (24.1 %), Staphylococcus spp. (20.7 %), and Micrococcus spp. (20.7 %) whereas for fungi species were Penicillium spp. (25 %), Cladosporium spp. (21.9 %), and Aspergillus spp. (21.9 %).


Soil Security ◽  
2021 ◽  
pp. 100008
Author(s):  
Debabrata Nath ◽  
Ranjan Laik ◽  
Vijay Singh Meena ◽  
Biswajit Pramanick ◽  
Santosh Kumar Singh

2019 ◽  
Vol 121 (2) ◽  
pp. 400-410 ◽  
Author(s):  
Rinaldo Botondi

PurposeThe purpose of this paper is to evaluate the possible microbiological contamination in terms of total counts and representative microbial groups found in the hazelnuts as well as in work areas and on the working surfaces of a hazelnut processing plant.Design/methodology/approachSome microbiological parameters related to the manufacturing process (e.g. aerobic colony count yeast and fungi) were evaluated. Indoor air samples were tested in order to evaluate the possible contamination of fungal strains (i.e. mycotoxin producing fungi).FindingsThe results showed that the highest values (>of 500 CFU/m3) for bacteria and fungi were only observed in the shelling and sorting areas. Some species such as Aspergillus and Penicillium (potential mycotoxin-producers) as well as some noxious colonies ofAspergillus fumigatuswere detected along the processing line. No occurrence of aflatoxins was observed in the finished product. Microbial loads obtained through surface analyses were in accordance to the reference data.Originality/valueThe aim of this study was to monitor and improve the procedures currently used in a hazelnut processing plant.


2017 ◽  
Vol 9 (11) ◽  
pp. 259
Author(s):  
Yu. I. Mitrofanov ◽  
G. Yu. Rabinovich

This paper presents the results of the research carried out in the All-Russian Research Institute of Reclaimed Lands. It has been established that land improvement techniques increased the integral index of physical soil conditions from 0.41-0.60 to 0.80-0.86. The soil demonstrated a greater total number of microorganisms, as well as certain physiological groups such as actinomycetes, autochthonous and especially nitrogen-fixers; but the number of fungi and denitrifiers, as well as the fungi causing the Fusarium wilt of the plants, decreased.Average crops increase achieved with the help of deep reclaiming band tillage of drained gleyish soil to depth 50-60 cm was 9.0-24.1% in both wet and dry years. This land improvement technique effect lasts for two-three years or longer. The ridged plowing compared to the ordinary plowing raised the spring crops by 0.55-0.61 ton/ha (by 16.9-17.5%) without making any additional expenditures.


2020 ◽  
Vol 10 (1) ◽  
pp. 43-48
Author(s):  
A. P. Paliy ◽  
S. O. Gujvinska ◽  
L. P. Livoshchenko ◽  
L. I. Nalivayko ◽  
Ye. M. Livoshchenko ◽  
...  

To maintain a stable composition of the gastrointestinal tract microflora in farm animals it is necessary to use probiotic agents to ensure the full functioning of the digestive, hormonal, and immune systems of the body. Most modern probiotics include lactic acid bacteria and bifidobacteria, which are the most physiologically valuable components of a healthy organism’s an indigenous microflora. The aim of this study was to provide indication and identification from the milk of healthy cows and gastric tract of healthy pigs and calves of the genus bacteria Lactobacillus, Bifidobacterium, and Lactococcus. The objects of research were cultures of microorganisms isolated from cows milk (82), the gastrointestinal tract of cattle (317), and piglets of different age groups (114). Bacteriological studies were carried out on the basis of the veterinary sanitation and parasitology laboratory of the National Scientific Center "Institute of Experimental and Clinical Veterinary Medicine" (Kharkiv) in accordance with current regulatory documents. According to the research of the gastrointestinal tract of clinically healthy calves and piglets isolated and typified to 317 and 114 cultures of microorganisms, the species composition of the microflora (82 bacterial cultures) of the cisternous and parenchymatous milk of clinically healthy cows was determined. A total of 513 isolates of microorganisms were isolated, including: Enterobacter spp. –2 (0,39%), Staphylococcus spp. – 7 (1,37%), Bacillus spp. – 11 (2,14%), Enterococcus spp. – 33 (6.43%), Lactococcus spp. – 75(14,62%), Bifidоbacterium spp. – 170 (33,14%), and Lactobacillus spp. – 215 (41,91%). In the study of the biological properties of isolated microorganisms Lactobacillus spp. (215) established their species identity: L. brevis – 7 (3.26%), L. delbrueckii – 9 (4,19%), L. acidophilus – 21 (9,77%), L. fermentum – 23 (10,69%), L. casei – 57 (26,51%), and L. plantarum – 98 (45.58%). Cultures of Bifidobacterium spp. (170) belong to B. suis – 2 (1,18%), B. breve – 7 (4,12%), B. lactentis – 15 (8,82%), B. bifidum – 21 (12,35%), B. longum – 22 (12,94%), B. infantis – 25 (14,71%), and B. adolescentis – 78 (45,88%). From samples of biological material of farm animals, 75 cultures of the genus Lactococcus spp. were isolated (75) of which Lactococcus lactis is representative. Isolated bacteria Lactobacillus spp., Bifidobacterium spp. and Lactococcus spp. promising when creating innovative probiotic products for farm animals.


Author(s):  
Muhammad Naveed ◽  
Syeda Sosan Bukhari ◽  
Adnan Mustafa ◽  
Allah Ditta ◽  
Saud Alamri ◽  
...  

Nickel (Ni) bioavailable fraction in the soil is of utmost importance because of its involvement in plant growth and environmental feedbacks. High concentrations of Ni in the soil environment, especially in the root zone, may retard plant growth that ultimately results in reduced plant biomass and yield. However, endophytic microorganisms have great potential to reduce the toxicity of Ni, especially when applied together with zeolite. The present research work was conducted to evaluate the potential effects of an endophytic bacterium Caulobacter sp. MN13 in combination with zeolite on the physiology, growth, quality, and yield of sesame plant under normal and Ni stressed soil conditions through possible reduction of Ni uptake. Surface sterilized sesame seeds were sown in pots filled with artificially Ni contaminated soil amended with zeolite. Results revealed that plant agronomic attributes such as shoot root dry weight, total number of pods, and 1000-grains weight were increased by 41, 45, 54, and 65%, respectively, over control treatment, with combined application of bacteria and zeolite in Ni contaminated soil. In comparison to control, the gaseous exchange parameters (CO2 assimilation rate, transpiration rate, stomatal- sub-stomatal conductance, chlorophyll content, and vapor pressure) were significantly enhanced by co-application of bacteria and zeolite ranging from 20 to 49% under Ni stress. Moreover, the combined utilization of bacteria and zeolite considerably improved water relations of sesame plant, in terms of relative water content (RWC) and relative membrane permeability (RMP) along with improvement in biochemical components (protein, ash, crude fiber, fat), and micronutrients in normal as well as in Ni contaminated soil. Moreover, the same treatment modulated the Ni-stress in plants through improvement in antioxidant enzymes (AEs) activities along with improved Ni concentration in the soil and different plant tissues. Correlation and principal component analysis (PCA) further revealed that combined application of metal-tolerant bacterium Caulobacter sp. MN13 and zeolite is the most influential strategy in alleviating Ni-induced stress and subsequent improvement in growth, yield, and physio-biochemical attributes of sesame plant.


2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 361-363
Author(s):  
E. Patkowska ◽  
D. Pięta ◽  
A. Pastucha

The purpose of the present studies was to establish the species composition of fungi occurring on the underground parts of winter wheat, Kobra cv. and to determine the quantitative and qualitative composition of microorganisms developing in the rhizosphere of this plant. The mycological analysis of the infected roots and the stem base of winter wheat gave 320 fungi isolates. Fusarium spp. were most frequently isolated, and their proportion was 64.7% of all the fungi. This genus was represented by F. avenaceum, F. culmorum, F. equiseti, F. graminearum, F. oxysporum and F. solani. Among these species the dominating ones were F. avenaceum (19.4%) and F. culmorum (38.7%). The microbiological analysis of winter wheat rhizosphere gave the highest number of total bacteria (6.32 × 106 cfu/g of d.w. of soil). The number of Pseudomonas pp. was 3.56 × 106 cfu/g of d.w. of soil, and the number of Bacillus spp. was 2.42 × 106 cfu/g of d.w. of soil. Fusarium spp. and Rhizoctonia solani dominated within pathogenic fungi isolated from the rhizosphere of winter wheat.


2020 ◽  
Vol 73 (1) ◽  
pp. 61
Author(s):  
Bhanu Priya ◽  
Abhishek Kumar ◽  
Neeraj Sharma

The new oxidovanadium(iv) complexes of composition [VO(3,5(NO2)2C6H2(OH)CONHO)2] 1 and [VO(acac)(3,5(NO2)2C6H2(OH)CONHO)] 2 (where acac=(CH3COCHCOCH3)–] have been synthesised by the reactions of VOSO4·5H2O and [VO(acac)2] with potassium 3,5-dinitrosalicylhydroxamate (3,5-(NO2)2SHK) and characterised by elemental analyses, molar conductivity, magnetic moment measurements and FT-IR, UV-vis, and electron spin resonance (ESR) spectroscopies and mass spectrometry. Infrared spectra of complexes have indicated bonding through oxygen atoms of carbonyl and hydroxamic groups (O,O coordination). The magnetic moment, ESR, and mass spectra of the complexes suggested their monomeric nature, and a distorted square-pyramidal geometry around the vanadium has tentatively been proposed. The electrochemical behaviour of 1 and 2 has been studied by cyclic voltammetry. Thermal behaviour of the complexes studied by thermogravimetric and differential thermal analysis techniques has yielded VO2 as the decomposition product. The invitro antimicrobial activity of the ligand and complexes has been assayed against pathogenic bacteria and fungi by the minimum inhibitory concentration (MIC) method. The invitro antioxidant activity of the complexes has been determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method.


2011 ◽  
Vol 20 (6) ◽  
pp. 607-617 ◽  
Author(s):  
Nor Husna Mat Hussin ◽  
Lye Munn Sann ◽  
Mariana Nor Shamsudin ◽  
Zailina Hashim

This study reports the types and concentrations of bacterial and fungal bioaerosols found in five randomly selected primary schools in Malaysia. Normal flora bacteria was the most frequently isolated bacteria including Staphylococcus spp., Pseudomonas spp. and Bacillus spp. Terribacillus spp. found in this study had never been reported before. The most frequently isolated fungal genera were Aspergillus, Penicillium, Fusarium, Rhizopus and Zygomycetes. The average concentration of bacteria in indoor and outdoor air were 1025 ± 612 CFU/m3 and 1473 ± 1261 CFU/m3, respectively, while the average concentration of fungal bioaerosol in indoor and outdoor air were 292 ± 83 CFU/m3 and 401 ± 235 CFU/m3, respectively. The percentages of bacterial and fungal samples that were within the American Conference of Industrial Hygenists (ACGIH) recommended levels were 44% and 33.8%, respectively. The ratio of indoor to outdoor fungi concentration was below 1.0, suggesting minimal indoor generative source for fungal bioaerosols. However, the ratio of indoor to outdoor bacteria concentration was approaching 1.0, suggesting the presence of potential internal generative source and inadequate ventilation. Building occupants might be one of the potential sources of bacteria in the indoor air as the bacteria concentrations without occupants were significantly lower than with occupants (p < 0.05).


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