Advances in application of dromedary camel tissue culture research

2019 ◽  
Keyword(s):  
Tissue Culture ◽  
Vaccine Development ◽  
Economic Value ◽  
Culture Technique ◽  
Cultural Value ◽  
Dromedary Camel ◽  
Treatment Efficiency ◽  
Dromedary Camels ◽  
Infection Dynamics ◽  
Shed Light

The dromedary camels have a great economic value in Asia and Africa, where they are kept for production of milk, meat, wool and leather; they are also used for transportation in some areas. Recently, the camel racing practice in the Middle East has added to the cultural value of dromedary camels, which lead to increased interest in improving their genetic makeup, reproductivity and treatment efficiency. Tissue culture-based therapy in domestic animals is described as safe with considerable welfares to the animals. Tissue culture application is currently growing in the fields of vaccine production, virus cultivation and study, cancer research, gene therapy, Immunological studies and molecular biology. In veterinary medicine tissue culture technique has been used for the study of viral infection dynamics and vaccine development and for treatment of many affection including musculoskeletal system injuries, liver disease and mastitis. However, in dromedary camels such applications are relatively less. The current work reviewed the available research on tissue culture in dromedary camels. Our review shed light on the therapeutic, genetic, preventive and reproductive contribution of tissue culture application in dromedary camels.

scholarly journals EFEKTIFITAS PENAMBAHAN MEDIA ORGANIK EKSTRAK UBI JALAR (Ipomoea batatas L) PADA PERTUMBUHAN SUBKULTUR ANGGREK Cattleya sp.

2017 ◽  
Vol 4 (1) ◽  
pp. 5
Author(s):  
Shinta Nurdika Meilani ◽  
Septarini Dian Anitasari ◽  
Fatimatuz Zuhro
Keyword(s):  
Tissue Culture ◽  
Sweet Potato ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Ipomoea Batatas ◽  
Economic Value ◽  
Conventional Technique ◽  
Culture Technique ◽  
Leaf Width ◽  
Number Of Leaves

<p align="center"> </p><p><em>Orchids (Chattleya sp) is popular plant which is favorted by peoples and has higt economic value. It’s price Rp. 562,000 / plant. Tissue culture technique is needed to produce this flower quicky than conventional technique. This study have aims to determine the effect of some medium concentration of organic sweet potato (Ipomoea batatas L) on growing Cattleya sp and for knowing the best of concentration on the growth of  Chattleya sp. </em><em>The method used was Completely Random Design (RAL) with four of factors, that is: 0 g / L, 75 g / L, 150 g / L, and 300 g / L. The results were analyzed with Anova test and Duncan test multiple ranger test at the level of 5%. Each treatment was repeated six times. Parameters were observed are the number of new shoots, long leaf, number of leaves, leaf width, long roots, and height of the plant for 5 weeks. Based on the results of analysis showed that the sweet potato extract on 150 g / L concentration give the best results in long root parameter, the concentration of 75 g / L gived the best results to long leaf parameter and concentration of 300 g / L gived the best effect against leaf width. Same concentrations of sweet potato did not give significantly effect to number of new shoots, plantlets height, and number of leaves. </em></p>

scholarly journals KONSENTRASI IAA (Indole Acetic Acid) DAN BAP (Benzyl Amino Purine) PADA KULTUR JARINGAN ULIN (Eusideroxylon zwageri)

2020 ◽  
Vol 7 (4) ◽  
Author(s):  
Herni Wahyuni ◽  
Reine Suci Wulandari ◽  
Muflihati Muflihati
Keyword(s):  
Tissue Culture ◽  
Indole Acetic Acid ◽  
Economic Value ◽  
Culture Technique ◽  
Optimum Concentration ◽  
Randomized Design ◽  
In The Wild ◽  
Completely Randomized Design ◽  
Benzyl Amino Purine ◽  
Eusideroxylon Zwageri

Ulin (Eusideroxylon zwageri) is a species of wood with high economic value that is difficult to find in the wild. Regeneration of E. zwageri is naturally very slow. Efforts to increase the procurement of E. zwageri seedlings can be done with a tissue culture technique. Through tissue culture, the seeds obtained will be uniform, have better quality and faster. This study aims to determine the effect and optimum concentration of IAA and BAP growth regulators on E. zwageri explants growth. The method used is experimental using a completely randomized design. Treatment factors are IAA concentration factor with 5 levels of treatment (0 mg/L, 0.5 mg/L, 1 mg/L, 1.5 mg/L, and 2 mg/L) and BAP concentration of 5 treatment levels (0 mg/L, 1 mg/L, 2 mg/L, 3 mg/L, and 4 mg/L). Each treatment was repeated 3 times. The results showed that the treatment of IAA concentration, BAP concentration and the interaction of IAA and BAP concentration affected the swelling of explants and growth of E. zwageri callus. The best concentration for callus explants was IAA of 2 mg/L and BAP of 0 mg/L, with a time of 12-13 weeks after planting.Keywords: BAP, E. zwageri, IAA, Tissue Culture

scholarly journals The Effect of Plant Growth Regulators on Callus Induction and Regeneration of Amygdalus communis

2011 ◽  
Vol 3 (3) ◽  
pp. 97-100
Author(s):  
Naimeh SHARIFMOGHADAM ◽  
Abbas SAFARNEJAD ◽  
Sayed Mohammad TABATABAEI
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Base Material ◽  
Culture Technique ◽  
Induction Medium ◽  
Root Induction ◽  
Amygdalus Communis

The Almond (Amygdalus communis) is one of the most important and oldest commercial nut crops, belonging to the Rosaceae family. Almond has been used as base material in pharmaceutical, cosmetic, hygienically and food industry. Propagation by tissue culture technique is the most important one in woody plants. In the current research, in vitro optimization of tissue culture and mass production of almond was investigated. In this idea, explants of actively growing shoots were collected and sterilized, then transferred to MS medium with different concentrations and combinations of plant growth regulators. The experiment was done in completely randomized blocks design, with 7 treatment and 30 replications. After 4 weeks, calli induction, proliferation, shoot length and number of shoot per explants were measured. Results showed that the best medium for shoot initiation and proliferation was MS + 0.5 mg/l IAA (Indol-3-Acetic Acid) + 1 mg/l BA (Benzyl Adenine). Autumn was the best season for collecting explants. The shoots were transferred to root induction medium with different concentrations of plant growth regulators. The best root induction medium was MS + 0.5 mg/l IBA (Indol Butyric Acid).

scholarly journals The effect of auxin 2,4-D and cytokinin 2-ip on direct somatic embryogenesis formation of Coffea arabica L. leaf explant

2011 ◽  
Vol 27 (2) ◽  
Author(s):  
Rina Arimarsetiowati
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Coffea Arabica ◽  
Callus Formation ◽  
Culture Technique ◽  
Plant Production ◽  
Direct Somatic Embryogenesis ◽  
Arabica Coffee ◽  
Randomized Design ◽  
Coffea Arabica L

One of the propagation technique for coffee plant production is tissue culture. Tissue culture technique for Coffea arabica L. faces some problems, mainly in the planlet formation regenerated from explants. The objective of this experiment was to examine the effect 2,4-D and 2-ip combination on the formation of direct somatic embryogenesis of Coffea arabica L. in leaves explant. Auxin (2,4-D) and cytokinin (2-ip) concentrations of, respectively, 1; 5 µM and 5; 10; 15; 20 were used as treatments. This research was conducted using completely randomized design with 10 replications. Observation to induce somatic embryos was done by quantitatively on number of callus from explant and number of embryogenic callus. Beside that, observation by qualitative descriptive was also done on deve lopment of embryogenesis. The results showed that Arabica coffee leaves explant of AS 2K clones could be induced in all medium combination except 5µM 2,4-D and 20µM 2-ip combination. Arabica coffee leaves explant of S 795, Sigararutang and AS 1 varieties could be induced in all medium combination. The highest frequency of callus formation was found in AS 2K, Sigararutang and AS 1 varieties on medium containing 1µM 2,4-D in combination with 10µM 2-ip, whereas for the S 795 variety on medium containing 5µM 2,4-D in combination with 10µM 2-ip. The highest frequency of embriogenic callus in all Arabica coffee variety could be reached on medium containing 5µM 2,4-D in combination with 15µM 2-ip. Key words : Coffea arabica L., somatic embryogenesis, 2,4-D, 2-ip, tissue culture, leaves, callus embryogenic.

scholarly journals KARAKTERISTIK BIOLOGI KUDA LAUT (Hippocampus spp) SEBAGAI PENGETAHUAN DASAR BUDIDAYANYA

OSEANA ◽  
2020 ◽  
Vol 45 (1) ◽  
pp. 70-81
Author(s):  
Dwi Eny Djoko Setyono
Keyword(s):  
Wild Population ◽  
Economic Value ◽  
Fishing Effort ◽  
Culture Technique ◽  
Ornamental Fish ◽  
Biological Characteristics ◽  
Basic Knowledge ◽  
Natural Growth ◽  
Steady Condition ◽  
Natural Medicine

Many species of marine organisms live in Indonesian waters have a potential for aquaculture (fishes, crustaceans, mollusc, echinoderm, and algae). They are cultured for food resources, the basis for cosmetic and natural medicine, and also as ornamental fish for marine aquarium. Seahorse (Syngnathidae: Hippocampus) is a marine fish which has high ecological and economic value. Fishing pressure of wild seahorse increased significantly when buyers offering an attractive price for the catch. High levels of fishing effort keep the wild stocks of seahorses well below the level that could be supported by natural growth and recruitment. Aquaculture for seahorses is very important to support sustainable production and to maintain the wild population in steady condition. Culture technique for seahorses has been developed in some countries, including Indonesia. However, seahorse aquaculture in Indonesia has not been achieved as expected due to the limited understanding of some biological characteristics. This manuscript deliberates some biological characteristics that useful as basic knowledge for seahorse aquaculture.

scholarly journals Production high class of micro tuber potato seeds (Solanum tuberosum L.). by Using tissue culture technique

2009 ◽  
Vol 3 (2) ◽  
pp. 99-106
Author(s):  
A.A. Al-jibouri ◽  
A.A. Al-salhay
Keyword(s):  
Tissue Culture ◽  
Solanum Tuberosum L ◽  
Culture Technique ◽  
Potato Cultivars ◽  
Condition Numbers ◽  
Mass Propagation ◽  
High Class ◽  
Dormancy Period ◽  
Elisa Technique

The aim of this investigation was produced micro tubers of four potato cultivars Premiere, Bintje, Estima and Escort in vitro. Apical meristems (0.2-0.4 mm) of potato cultivars were excised and cultured on nutrient medium and incubated at 24±2 Cº and 1000 lux light intensity for 16 hrs per day. The developing plantlets were examined serological by using ELISA technique to eliminate the viral infected plantlets. The virus-free plantlets were chopped into pieces with single bud and re cultured on fresh medium for mass propagation. For micro tubers formation in test tubes, the cultures were transferred to another medium containing a high percent of sucrose (60g/L) with different concentrations of kinetin; the cultures were incubated under 16±2 Cº and 8 hrs photoperiod. The plantlets formed micro tubers after 8-10 weeks from culturing. The results showed significant differences among cultivar’s in their response to in vitro culture and micro tubers formation. The results also showed that the kinetin concentration had significant effect on micro tubers, and 1mg/l kinetin concentration was the best. The micro tubers were stored for 10 week at 4Cº to break down the dormancy period, and gave 100% germination under nursery condition. Numbers of tubers derived from micro tubers and normal tubers of these cultivars were compared at the end of season.

scholarly journals Initiation of Ipomoea batatas Through Tissue Culture Technique

2021 ◽  
Vol 12 (2) ◽  
pp. 79-83
Author(s):  
Mahmoud Ali ◽  
Ali Abido ◽  
Mohamed El-Torky ◽  
Badria Hassan ◽  
Marwa Abdelwahab
Keyword(s):  
Tissue Culture ◽  
Ipomoea Batatas ◽  
Culture Technique ◽  
Tissue Culture Technique

scholarly journals The effects of differences in water exchange on the productivity of glass eel stage in Anguilla bicolor bicolor culture

2021 ◽  
Vol 322 ◽  
pp. 02006
Author(s):  
Eri Setiadi ◽  
Adang Saputra ◽  
Imam Taufik
Keyword(s):  
Water Quality ◽  
Blood Glucose ◽  
Survival Rate ◽  
Quality Management ◽  
Water Exchange ◽  
Water Quality Management ◽  
Economic Value ◽  
Culture Technique ◽  
Glass Eel ◽  
Survival And Growth

Eel, Anguilla bicolor bicolor has an economic value for export commodity. Eel farming has been developed both of home to industries scales. However, the main problem during the glass eel culture is the low survival rate, characterized by high mortality. This condition is related to the culture technique that has not been established yet, especially water quality management. The research aims to determine the best water exchange during the culture of the glass eel on the survival, growth, and blood glucose. The different percentages of water exchange as a treatment were followed A) control (without water exchange); B) 20% of water exchange; C) 40% of water exchange; and D) 60% of water exchange. The water exchange has affected the survival, growth, and blood glucose (P<0.05). The ammonia, nitrite, and nitrate values during the culture period showed that the control was higher compared to the others. The water exchange of 40% is suitable for glass eel stage culture to obtain the best survival and growth.

scholarly journals Mass Propagation of Nauclea diderrichii (de Willd. & Th. Dur) Merr. Seedlings through Tissue Culture Technique

2021 ◽  
Vol 31 (1) ◽  
pp. 51-60
Author(s):  
RI Oyediran ◽  
JO Afolabi ◽  
DB Olomola ◽  
FO Akanni
Keyword(s):  
Tissue Culture ◽  
Adventitious Shoots ◽  
Basal Medium ◽  
Culture Technique ◽  
Seedling Production ◽  
Mass Propagation ◽  
In Vitro Plantlets ◽  
Number Of Leaves ◽  
Nauclea Diderrichii

Nauclea diderrichii is a tree species of economic importance. However, its plantation establishment is limited by inadequate seedling production. Hence, there is ample scope of tissue culture for its mass propagation. Its in vitro plantlets development as affected by media strengths indicated that 100 % seed germination was obtained in full MS basal medium while the least (3.35 %) was from quarter-strength at 8 Weeks after inoculation (WAI). The effects of BAP and NAA assessed on the growth of its sub-cultured plantlets showed that highest number of leaves (17) and adventitious shoots (3) were obtained from MS basal medium supplemented with 0.1 mg/l BAP only. Whereas, highest shoot length (3.61 cm) and average number of roots (5/plantlet) were obtained from the same medium without hormone(s) at 8 WAI. Further sub-culturing into MS with 0.05 mg/l NAA resulted into plantlets having optimum shoot and massive root growth ready for acclimatization in 6 WAI. The plantlets were successfully acclimatized using coconuthusk/ topsoil mixture with 90 % survival. Plant Tissue Cult. & Biotech. 31(1): 51-60, 2021 (June)

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