scholarly journals Planar-bilayer activities of linear oligoester bolaamphiphiles

2011 ◽  
Vol 7 ◽  
pp. 1562-1569 ◽  
Author(s):  
Jonathan K W Chui ◽  
Thomas M Fyles ◽  
Horace Luong
Keyword(s):  
Ion Transport ◽  
Voltage Clamp ◽  
Grid Method ◽  
Planar Bilayer ◽  
Transport Activity ◽  
Planar Bilayers ◽  
Voltage Clamp Technique ◽  
Clamp Technique ◽  
Voltage Dependent ◽  
Related Compounds

Voltage-clamp experiments of eight oligoester bolaamphiphiles in two subclasses are described. Syntheses of three new terephthalate-based compounds were achieved in three linear steps. Together with five previously described, related compounds, the ion transport activity was assessed by means of the voltage-clamp technique. All of the compounds show multiple types of conductance behavior in planar bilayers, a subset of which was exponentially voltage-dependent. The varied and irregular activities were summarized with the aid of a recently developed “activity-grid” method.

Adjusting the neurons models in neuromimetic ICs using the voltage-clamp technique

2008 ◽  
Author(s):  
Sylvain Saighi ◽  
Laure Buhry ◽  
Yannick Bornat ◽  
Gilles N'Kaoua ◽  
Jean Tomas ◽  
...  
Keyword(s):  
Voltage Clamp ◽  
Voltage Clamp Technique ◽  
Clamp Technique

scholarly journals Membrane properties of a barnacle photoreceptor examined by the voltage clamp technique

1970 ◽  
Vol 208 (2) ◽  
pp. 385-413 ◽  
Author(s):  
H. Mack Brown ◽  
S. Hagiwara ◽  
H. Koike ◽  
R. M. Meech
Keyword(s):  
Voltage Clamp ◽  
Membrane Properties ◽  
Voltage Clamp Technique ◽  
Clamp Technique

Characteristics of L- and T-type Ca2+ currents in canine cardiac Purkinje cells

1989 ◽  
Vol 256 (5) ◽  
pp. H1478-H1492 ◽  
Author(s):  
Y. Hirano ◽  
H. A. Fozzard ◽  
C. T. January
Keyword(s):  
Kinetic Parameters ◽  
Voltage Clamp ◽  
Purkinje Cells ◽  
Current Density ◽  
Voltage Dependence ◽  
Cell Voltage ◽  
Voltage Clamp Technique ◽  
Recovery From Inactivation ◽  
Cardiac Purkinje Cells ◽  
Voltage Dependent

Two types of Ca2+ currents were recorded in single dialyzed canine cardiac Purkinje cells using a whole cell voltage clamp technique. T-type current was easily separated from L-type current, because its voltage dependence of inactivation and activation was more negative and it decayed rapidly. L-type current was available at more depolarized holding potentials, activated at more positive voltages, and decayed slowly. In 2 mM extracellular Ca2+ concentration [( Ca]o), the average peak T- and L-type current density was 1.70 and 2.87 pA/pF, respectively. T-type current was relatively insensitive to modification by Ca2+, nifedipine, Cd2+, BAY K 8644, or isoproterenol. T-type current was more sensitive to block by Ni2+ and amiloride. Replacement of Ca2+ by Ba2+ or Sr2+ did not increase T-type current. Changes in the Ca2+ or Ba2+ concentration caused parallel shifts in the voltage dependence of several kinetic parameters for L- and T-type current. In 2 mM [Ca]o, the V1/2 (Boltzmann fit) for inactivation of T-type current was -68 mV with a slope of 3.9, and for L-type current the V1/2 was -31 mV with a slope of 5.5. Recovery from inactivation of L- and T-type current was voltage dependent, and for similar conditions L-type current recovered from inactivation more rapidly than T-type current. These findings show that T- and L-type currents are large in cardiac Purkinje cells, and they can easily be separated by their voltage, kinetic, and pharmacological differences. Both may have important physiological roles.

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