scholarly journals ROMP-Derived cyclooctene-based monolithic polymeric materials reinforced with inorganic nanoparticles for applications in tissue engineering

2010 ◽  
Vol 6 ◽  
pp. 1199-1205 ◽  
Author(s):  
Franziska Weichelt ◽  
Solvig Lenz ◽  
Stefanie Tiede ◽  
Ingrid Reinhardt ◽  
Bernhard Frerich ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stromal Cells ◽  
Ring Opening ◽  
Chemical Properties ◽  
Polymeric Materials ◽  
Human Adipose Tissue ◽  
Calcium Hydroxyapatite ◽  
Inorganic Nanoparticles ◽  
Physico Chemical ◽  
Scaffold Materials

Porous monolithic inorganic/polymeric hybrid materials have been prepared via ring-opening metathesis copolymerization starting from a highly polar monomer, i.e., cis-5-cyclooctene-trans-1,2-diol and a 7-oxanorborn-2-ene-derived cross-linker in the presence of porogenic solvents and two types of inorganic nanoparticles (i.e., CaCO3 and calcium hydroxyapatite, respectively) using the third-generation Grubbs initiator RuCl2(Py)2(IMesH2)(CHPh). The physico-chemical properties of the monolithic materials, such as pore size distribution and microhardness were studied with regard to the nanoparticle type and content. Moreover, the reinforced monoliths were tested for the possible use as scaffold materials in tissue engineering, by carrying out cell cultivation experiments with human adipose tissue-derived stromal cells.

scholarly journals Graphene and its derivatives as biomedical materials: future prospects and challenges

2018 ◽  
Vol 8 (3) ◽  
pp. 20170056 ◽  
Author(s):  
Arghya Narayan Banerjee
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Graphene Oxide ◽  
Cell Growth ◽  
Drug Loading ◽  
Chemical Properties ◽  
Culture Cell ◽  
Biomedical Materials ◽  
Physico Chemical ◽  
Bio Imaging

Graphene and its derivatives possess some intriguing properties, which generates tremendous interests in various fields, including biomedicine. The biomedical applications of graphene-based nanomaterials have attracted great interests over the last decade, and several groups have started working on this field around the globe. Because of the excellent biocompatibility, solubility and selectivity, graphene and its derivatives have shown great potential as biosensing and bio-imaging materials. Also, due to some unique physico-chemical properties of graphene and its derivatives, such as large surface area, high purity, good bio-functionalizability, easy solubility, high drug loading capacity, capability of easy cell membrane penetration, etc., graphene-based nanomaterials become promising candidates for bio-delivery carriers. Besides, graphene and its derivatives have also shown interesting applications in the fields of cell-culture, cell-growth and tissue engineering. In this article, a comprehensive review on the applications of graphene and its derivatives as biomedical materials has been presented. The unique properties of graphene and its derivatives (such as graphene oxide, reduced graphene oxide, graphane, graphone, graphyne, graphdiyne, fluorographene and their doped versions) have been discussed, followed by discussions on the recent efforts on the applications of graphene and its derivatives in biosensing, bio-imaging, drug delivery and therapy, cell culture, tissue engineering and cell growth. Also, the challenges involved in the use of graphene and its derivatives as biomedical materials are discussed briefly, followed by the future perspectives of the use of graphene-based nanomaterials in bio-applications. The review will provide an outlook to the applications of graphene and its derivatives, and may open up new horizons to inspire broader interests across various disciplines.

Poly(1,3-Butylene Fumerate) and Poly(1,3-Butylene Fumerate)-co-(1,3-Butylene Maleate) as Electrospun Scaffold Materials

2009 ◽  
Vol 1239 ◽  
Author(s):  
Kirsten Nicole Cicotte ◽  
Shawn M. Dirk ◽  
Elizabeth Hedberg-Dirk
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Ring Opening ◽  
Room Temperature ◽  
Electrospinning Process ◽  
Crosslinking Reaction ◽  
Electrospun Scaffold ◽  
Fiber Mats ◽  
Nano Fiber ◽  
Scaffold Materials

AbstractPoly(butylene fumerate) (PBF) and poly(butylene fumerate)-co-(butylene maleate) (PBFcBM) have been synthesized from the ring opening and condensation reactions of maleic anhydride (MA) and 1,3-butanediol (BD). PBFcBM synthesized in this way contains greater than 85% maleate groups. Both PBF and PBFcBM have a glass transition temperature (Tg) below room temperature and therefore cannot be electrospun using the conventional electrospinning process as a non-porous film results. To facilitate production of nonwoven micro- and nano-fiber mats, a UV-source (λ=356 nm) was used in combination with a photoinitator loaded polymer solution to initiate the crosslinking reaction of the fumerate and maleate functional groups as the fibers were produced. The resulting non-woven fiber mats are potentially suitable scaffolds for tissue engineering and drug delivery application.

scholarly journals Mitigating the non-specific uptake of immunomagnetic microparticles enables the extraction of endothelium from human fat

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Jeremy A. Antonyshyn ◽  
Vienna Mazzoli ◽  
Meghan J. McFadden ◽  
Anthony O. Gramolini ◽  
Stefan O. P. Hofer ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Adipose Tissue ◽  
Stromal Cells ◽  
Reliable Method ◽  
Vascular Tissue ◽  
Human Adipose Tissue ◽  
Vascular Tissue Engineering ◽  
Microvascular Endothelial Cells ◽  
Specific Uptake

AbstractEndothelial cells are among the fundamental building blocks for vascular tissue engineering. However, a clinically viable source of endothelium has continued to elude the field. Here, we demonstrate the feasibility of sourcing autologous endothelium from human fat – an abundant and uniquely dispensable tissue that can be readily harvested with minimally invasive procedures. We investigate the challenges underlying the overgrowth of human adipose tissue-derived microvascular endothelial cells by stromal cells to facilitate the development of a reliable method for their acquisition. Magnet-assisted cell sorting strategies are established to mitigate the non-specific uptake of immunomagnetic microparticles, enabling the enrichment of endothelial cells to purities that prevent their overgrowth by stromal cells. This work delineates a reliable method for acquiring human adipose tissue-derived microvascular endothelial cells in large quantities with high purities that can be readily applied in future vascular tissue engineering applications.

Physico-chemical properties and degradability of non-woven hyaluronan benzylic esters as tissue engineering scaffolds

Biomaterials ◽  
2002 ◽  
Vol 23 (4) ◽  
pp. 1053-1063 ◽  
Author(s):  
E Milella ◽  
E Brescia ◽  
C Massaro ◽  
P.A Ramires ◽  
M.R Miglietta ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Chemical Properties ◽  
Tissue Engineering Scaffolds ◽  
Physico Chemical

Cartilaginous Tissue Formation with an Elastic PLCL Scaffold and Human Adipose Tissue-Derived Stromal Cells

2007 ◽  
Vol 342-343 ◽  
pp. 385-388
Author(s):  
So Eun Lee ◽  
Young Mee Jung ◽  
Soo Hyun Kim ◽  
Sang Heon Kim ◽  
Jong Won Rhie ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Adipose Tissue ◽  
Stromal Cells ◽  
Cartilage Tissue Engineering ◽  
Human Adipose Tissue ◽  
Cartilage Tissue ◽  
Tissue Formation ◽  
Cartilaginous Tissue

In cartilage tissue engineering, as a cell source, adult stem cells are very attractive for clinical applications. Recent studies suggest that human adipose tissue-derived stromal cells (ASCs) have multilineage potential similar to bone marrow-derived stromal cells (BMSCs). ASCs are obtained from adipose tissue easily isolated by suction-assisted lipectomy in various body parts. Also, as one of major factors of cartilage tissue engineering, scaffolds have an important role in cartilage formation. Poly(L-lactide-co-ε-carprolactone) scaffolds have physiological activity, biodegradability, high cell affinity, and mechano-activity. The object of this study is cartilaginous tissue formation using highly elastic PLCL scaffolds and ASCs in vitro and in vivo. Poly(L-lactide-co-ε-carprolactone) copolymers were synthesized from lactide and ε-carprolactone in the presence of stannous octoate as catalyst. The scaffolds with 85% porosity and 300-500μm pore size were fabricated by gel-pressing method. ASCs were seeded on scaffolds and cultured for 21days in vitro. Cell/polymer constructs were characterized by reverse transcriptase-polymerase chain reaction for confirming differentiation to chondrocytes onto PLCL scaffolds. Also, for examining cartilaginous tissue formation in vivo, ASCs seeded scaffolds which were induced chondrogenesis for 2 weeks were implanted in nude mice subcutaneously for up to 8weeks. Histological studies showed that implants partially developed cartilaginous tissue within lacunae. And there was an accumulation of sulfated glycoaminoglycans. Immunohistochemical analysis revealed that implants were positively stained for specific extracellular matrix. These results indicate that ASCs and PLCL scaffols could be used to cartilage tissue engineering.

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