scholarly journals Development of novel cyclic NGR peptide–daunomycin conjugates with dual targeting property

2018 ◽  
Vol 14 ◽  
pp. 911-918 ◽  
Author(s):  
Andrea Angelo Pierluigi Tripodi ◽  
Szilárd Tóth ◽  
Kata Nóra Enyedi ◽  
Gitta Schlosser ◽  
Gergely Szakács ◽  
...  
Keyword(s):  
Tumor Therapy ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Drug Conjugates ◽  
Dual Targeting ◽  
Adenocarcinoma Cells ◽  
Ngr Peptide ◽  
Control Compound ◽  
Ht 29 ◽  
Colon Adenocarcinoma Cells

Cyclic NGR peptides as homing devices are good candidates for the development of drug conjugates for targeted tumor therapy. In our previous study we reported that the Dau=Aoa-GFLGK(c[KNGRE]-GG-)-NH2 conjugate has a significant antitumor activity against both CD13+ HT-1080 human fibrosarcoma and CD13− but integrin positive HT-29 human colon adenocarcinoma cells. However, it seems that the free ε-amino group of Lys in the cycle is not necessary for the biological activity. Therefore, we developed novel cyclic NGR peptide–daunomycin conjugates in which Lys was replaced by different amino acids (Ala, Leu, Nle, Pro, Ser). The exchange of the Lys residue in the cycle simplified the cyclization step and resulted in a higher yield. The new conjugates showed lower chemostability against deamidation of Asn than the control compound, thus they had lower selectivity to CD13+ cells. However, the cellular uptake and cytotoxic effect of Dau=Aoa-GFLGK(c[NleNGRE]-GG-)-NH2 was higher in comparison to the control especially on HT-29 cells. Therefore, this conjugate is more suitable for drug targeting with dual targeting property.

scholarly journals Effect of β-carotene-rich tomato lycopene β-cyclase (tlcy-b) on cell growth inhibition in HT-29 colon adenocarcinoma cells

2008 ◽  
Vol 102 (2) ◽  
pp. 207-214 ◽  
Author(s):  
Paola Palozza ◽  
Diana Bellovino ◽  
Rossella Simone ◽  
Alma Boninsegna ◽  
Francesco Cellini ◽  
...  
Keyword(s):  
Cell Growth ◽  
Growth Inhibition ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Cell Growth Inhibition ◽  
Adenocarcinoma Cells ◽  
Ht 29 ◽  
Colon Adenocarcinoma Cells ◽  
Β Carotene

Lycopene β-cyclase (tlcy-b) tomatoes, obtained by modulating carotenogenesis via genetic engineering, contain a large amount of β-carotene, as clearly visible by their intense orange colour. In the present study we have subjected tlcy-b tomatoes to an in vitro simulated digestion and analysed the effects of digestate on cell proliferation. To this aim we used HT-29 human colon adenocarcinoma cells, grown in monolayers, as a model. Digested tomatoes were diluted (20 ml, 50 ml and 100 ml/l) in culture medium and added to the cells for different incubation times (24 h, 48 h and 72 h). Inhibition of cell growth by tomato digestate was dose-dependent and resulted from an arrest of cell cycle progression at the G0/G1 and G2/M phase and by apoptosis induction. A down-regulation of cyclin D1, Bcl-2 and Bcl-xl expression was observed. We also found that heat treatment of samples before digestion enhanced β-carotene release and therefore cell growth inhibition. To induce with purified β-carotene solubilised in tetrahydrofuran the same cell growth inhibition obtained with the tomato digestate, a higher amount of the carotenoid was necessary, suggesting that β-carotene micellarised during digestion is utilised more efficiently by the cells, but also that other tomato molecules, reasonably made available during digestion, may be present and cooperate with β-carotene in promoting cell growth arrest.

The proliferative response of HT-29 human colon adenocarcinoma cells to bombesin-like peptides

2001 ◽  
Vol 172 (2) ◽  
pp. 151-157 ◽  
Author(s):  
Giuseppe Cassano ◽  
Nicoletta Resta ◽  
Giuseppe Gasparre ◽  
Claudio Lippe ◽  
Ginevra Guanti
Keyword(s):  
Proliferative Response ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Adenocarcinoma Cells ◽  
Ht 29 ◽  
Human Colon Adenocarcinoma ◽  
Colon Adenocarcinoma Cells

Isolation and anticancer effect of brucine in human colon adenocarcinoma cells HT-29

2021 ◽  
Vol 17 (74) ◽  
pp. 367
Author(s):  
Jianping Zhao ◽  
Zhenyu Feng ◽  
Shuang Meng ◽  
Xiaorong Zhou ◽  
Xiaojuan Ma ◽  
...  
Keyword(s):  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Anticancer Effect ◽  
Adenocarcinoma Cells ◽  
Ht 29 ◽  
Human Colon Adenocarcinoma ◽  
Colon Adenocarcinoma Cells

scholarly journals Epigallocatechin-3-gallate-induced stress signals in HT-29 human colon adenocarcinoma cells

2003 ◽  
Vol 24 (8) ◽  
pp. 1369-1378 ◽  
Author(s):  
C. Chen ◽  
G. Shen ◽  
V. Hebbar ◽  
R. Hu ◽  
E. D. Owuor ◽  
...  
Keyword(s):  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Induced Stress ◽  
Adenocarcinoma Cells ◽  
Stress Signals ◽  
Ht 29 ◽  
Human Colon Adenocarcinoma ◽  
Colon Adenocarcinoma Cells

Effect of prostaglandin E2 on the proliferation, Ca2+ mobilization and cAMP in HT-29 human colon adenocarcinoma cells

2000 ◽  
Vol 152 (2) ◽  
pp. 217-222 ◽  
Author(s):  
Giuseppe Cassano ◽  
Giuseppe Gasparre ◽  
Francesco Susca ◽  
Claudio Lippe ◽  
Ginevra Guanti
Keyword(s):  
Prostaglandin E2 ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Adenocarcinoma Cells ◽  
Ht 29 ◽  
Human Colon Adenocarcinoma ◽  
Colon Adenocarcinoma Cells
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