scholarly journals Development of a fluorogenic small substrate for dipeptidyl peptidase-4

2017 ◽  
Vol 13 ◽  
pp. 2690-2697 ◽  
Author(s):  
Futa Ogawa ◽  
Masanori Takeda ◽  
Kanae Miyanaga ◽  
Keita Tani ◽  
Ryuji Yamazawa ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Organic Compounds ◽  
Reaction Medium ◽  
Dipeptidyl Peptidase ◽  
Fluorogenic Substrate ◽  
Fluorometric Determination ◽  
Dipeptidyl Peptidase 4 ◽  
Fluorogenic Probes ◽  
Hydrolysis Of

A series of aniline and m-phenylenediamine derivatives with electron-withdrawing 3,3,3-trifluoropropenyl substituents were synthesized as small and chemically stable fluorescent organic compounds. Their fluorescence performances were evaluated by converting 2,4-disubstituted aniline 1 to the non-fluorescent dipeptide analogue H-Gly-Pro-1 for the use as a fluorogenic substrate for dipeptidyl peptidase-4 (DPP-4). The progress of the enzymatic hydrolysis of H-Gly-Pro-1 with DPP-4 was monitored by fluorometric determination of 1 released into the reaction medium. The results suggest that 1 could be used as fluorophore in OFF–ON-type fluorogenic probes.

The determination of urea in soil extracts and related samples—a review

Soil Research ◽  
2004 ◽  
Vol 42 (7) ◽  
pp. 709 ◽  
Author(s):  
David F. Lambert ◽  
John E. Sherwood ◽  
Paul S. Francis
Keyword(s):  
Enzymatic Hydrolysis ◽  
Flow Injection Analysis ◽  
Acidic Solution ◽  
Clinical Applications ◽  
Urease Inhibitors ◽  
Viable Option ◽  
Soil Extracts ◽  
Diacetyl Monoxime ◽  
Hydrolysis Of

Although the dominant methods for the determination of urea in clinical applications incorporate selective enzymatic hydrolysis of urea, the determination of urea in soil extracts is complicated by the presence of urease inhibitors. The spectrophotometric determination of urea with an acidic solution diacetyl monoxime and semicarbazide is a viable option but traditional manual procedures are time-consuming. New variations on these procedures, based on microplates or flow-injection analysis methodologies, allow a far greater number of samples to be analysed with high precision and sensitivity.

scholarly journals Determination of inhibition in the enzymatic hydrolysis of cellobiose using hybrid neural modeling

2005 ◽  
Vol 22 (1) ◽  
pp. 19-29 ◽  
Author(s):  
F. C. Corazza ◽  
L. P. V. Calsavara ◽  
F. F. Moraes ◽  
G. M. Zanin ◽  
I. Neitzel
Keyword(s):  
Enzymatic Hydrolysis ◽  
Neural Modeling ◽  
Hydrolysis Of

A miniaturized microtiter plate protocol for the determination of selenomethionine in selenized yeast via enzymatic hydrolysis of protein-bound selenium

2011 ◽  
Vol 3 (3) ◽  
pp. 738 ◽  
Author(s):  
Michael Stiboller ◽  
Markus Damm ◽  
Allycia M. Barbera ◽  
Doris Kuehnelt ◽  
Kevin A. Francesconi ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Microtiter Plate ◽  
Hydrolysis Of ◽  
Selenized Yeast

Selecting the right blood glucose monitor for the determination of glucose during the enzymatic hydrolysis of corncob pretreated with different methods

2011 ◽  
Vol 102 (20) ◽  
pp. 9646-9652 ◽  
Author(s):  
Erinc Bahcegul ◽  
Emre Tatli ◽  
Nazife Isik Haykir ◽  
Serpil Apaydin ◽  
Ufuk Bakir
Keyword(s):  
Blood Glucose ◽  
Enzymatic Hydrolysis ◽  
Blood Glucose Monitor ◽  
The Right ◽  
Hydrolysis Of

Sequence Determination of an Antioxidant Peptide Obtained by Enzymatic Hydrolysis of Oyster Crassostrea madrasensis (Preston)

2016 ◽  
Vol 22 (3) ◽  
pp. 421-433 ◽  
Author(s):  
K. K. Asha ◽  
K. R. Remya Kumari ◽  
K. Ashok Kumar ◽  
Niladri S. Chatterjee ◽  
R. Anandan ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sequence Determination ◽  
Antioxidant Peptide ◽  
Hydrolysis Of
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