scholarly journals Biantennary oligoglycines and glyco-oligoglycines self-associating in aqueous medium

2014 ◽  
Vol 10 ◽  
pp. 1372-1382 ◽  
Author(s):  
Svetlana V Tsygankova ◽  
Alexander A Chinarev ◽  
Alexander B Tuzikov ◽  
Nikolai Severin ◽  
Alexey A Kalachev ◽  
...  
Keyword(s):  
Hydrogen Bonding ◽  
Sialic Acid ◽  
Antiviral Activity ◽  
Aqueous Medium ◽  
General Formula ◽  
Driving Force ◽  
Polypeptide Chain ◽  
Chain Conformation ◽  
Mica Surface ◽  
Antenna Length

Oligoglycines designed in a star-like fashion, so-called tri- and tetraantennary molecules, were found to form highly ordered supramers in aqueous medium. The formation of these supramers occurred either spontaneously or due to the assistance of a mica surface. The driving force of the supramer formation is hydrogen bonding, the polypeptide chain conformation is related to the folding of helical polyglycine II (PG II). Tri- and tetraantennary molecules are capable of association if the antenna length reach 7 glycine (Gly) residues. Properties of similar biantennary molecules have not been investigated yet, and we compared their self-aggregating potency with similar tri- and tetraantennary analogs. Here, we synthesized oligoglycines of the general formula R-Gly n -Х-Gly n -R (X = -HN-(СН2) m -NH-, m = 2, 4, 10; n = 1–7) without pendant ligands (R = H) and with two pendant sialoligands (R = sialic acid or sialooligosaccharide). Biantennary oligoglycines formed PG II aggregates, their properties, however, differ from those of the corresponding tri- and tetraantennary oligoglycines. In particular, the tendency to aggregate starts from Gly4 motifs instead of Gly7. The antiviral activity of end-glycosylated peptides was studied, and all capable of assembling glycopeptides demonstrated an antiviral potency which was up to 50 times higher than the activity of peptide-free glycans.

Sialic Acid Dependent Polypeptide Chain Heterogeneity of Human Fibrinogen Demonstrated by Two-Dimensional Electrophoresis

1982 ◽  
Vol 47 (01) ◽  
pp. 019-021 ◽  
Author(s):  
Cemal Kuyas ◽  
André Haeberli ◽  
P Werner Straub
Keyword(s):  
Sialic Acid ◽  
Polypeptide Chain ◽  
Two Dimensional ◽  
Charge Heterogeneity ◽  
Human Fibrinogen ◽  
Two Dimensional Electrophoresis ◽  
Isoelectric Points ◽  
Polypeptide Chains ◽  
Dimensional Electrophoresis ◽  
Chain Type

SummaryHuman fibrinogen was compared with asialofibrinogen by two-dimensional electrophoresis to evaluate the contribution of sialic acid to the heterogeneity of the γ- and Bβ-polypeptide chains.Reduced fibrinogen showed three major variants for both the γ- and Bβ-chains. In addition two minor γ-bands with a more acidic isoelectric point than the normal γ-chains were observed. Electrophoresis in the second dimension (SDS) suggests that these most acidic bands are γ-chain-variants with a higher molecular weight. In asialofibrinogen only two predominant variants with more alkaline isoelectric points were present in each chain type.It is concluded that enzymatic removal of sialic acid partially reduces the heterogeneity of the γ- and Bβ-polypeptide chains of human fibrinogen, but additional sources producing charge heterogeneity must be sought.

scholarly journals Potent sialic acid inhibitors that target influenza A virus hemagglutinin

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yu-Jen Chang ◽  
Cheng-Yun Yeh ◽  
Ju-Chien Cheng ◽  
Yu-Qi Huang ◽  
Kai-Cheng Hsu ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Antiviral Activity ◽  
Binding Site ◽  
Influenza A Virus ◽  
Receptor Binding ◽  
Influenza A ◽  
The United States ◽  
Ligand Complex ◽  
Receptor Binding Site ◽  
Computational Strategy

AbstractEradicating influenza A virus (IAV) is difficult, due to its genetic drift and reassortment ability. As the infectious cycle is initiated by the influenza glycoprotein, hemagglutinin (HA), which mediates the binding of virions to terminal sialic acids moieties, HA is a tempting target of anti-influenza inhibitors. However, the complexity of the HA structure has prevented delineation of the structural characterization of the HA protein–ligand complex. Our computational strategy efficiently analyzed > 200,000 records of compounds held in the United States National Cancer Institute (NCI) database and identified potential HA inhibitors, by modeling the sialic acid (SA) receptor binding site (RBS) for the HA structure. Our modeling revealed that compound NSC85561 showed significant antiviral activity against the IAV H1N1 strain with EC50 values ranging from 2.31 to 2.53 µM and negligible cytotoxicity (CC50 > 700 µM). Using the NSC85561 compound as the template to generate 12 derivatives, robust bioassay results revealed the strongest antiviral efficacies with NSC47715 and NSC7223. Virtual screening clearly identified three SA receptor binding site inhibitors that were successfully validated in experimental data. Thus, our computational strategy has identified SA receptor binding site inhibitors against HA that show IAV-associated antiviral activity.

Polypeptide chain conformation in feather keratin

1965 ◽  
Vol 14 (1) ◽  
pp. 279-282 ◽  
Author(s):  
R.D.B. Fraser ◽  
E. Suzuki
Keyword(s):  
Polypeptide Chain ◽  
Chain Conformation ◽  
Feather Keratin

scholarly journals α-NH4Fe(HAsO4)2

2014 ◽  
Vol 70 (6) ◽  
pp. i21-i22 ◽  
Author(s):  
Najoua Ouerfelli ◽  
Amira Souilem ◽  
Mohamed Faouzi Zid ◽  
Ahmed Driss
Keyword(s):  
Hydrogen Bonding ◽  
Ion Exchange ◽  
Coordination Number ◽  
General Formula ◽  
Title Compound ◽  
Three Dimensional ◽  
Coordination Polyhedra ◽  
Structural Relationships ◽  
Ammonium Iron

The title compound α-ammonium iron(III) bis[hydrogen arsenate(V)], α-NH4Fe(HAsO4)2(or poly[ammonium bis(μ-hydrogen arsenato)ferrate(III)], {NH4[Fe(HAsO4)2]}n), synthesized hydrothermally, is isostructural with NH4Fe(HPO4)2. Condensation of the hydrogen arsenate groups with FeO6coordination octahedraviacommon corners results in an overall three-dimensional framework containing interconnected channels parallel to thea-,b-andc-axis directions. The NH4+cations are located in three intersecting tunnels, which is promising as an ion exchange. Hydrogen bonding of the types O—H...O and N—H...O consolidates the packing of the structure. The distortion of the coordination polyhedra is analyzed by means of the effective coordination number and distortion indices. Structural relationships with other compounds of general formulaMIMIII[HXO4)]2(X= P, As) are discussed.

scholarly journals Sialic acid involves in the interaction between ovomucin and hemagglutinin and influences the antiviral activity of ovomucin

2018 ◽  
Vol 119 ◽  
pp. 533-539 ◽  
Author(s):  
Qi Xu ◽  
Yuanyuan Shan ◽  
Ning Wang ◽  
Yaping Liu ◽  
Maojie Zhang ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Antiviral Activity
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