scholarly journals Interaction of ghrelin and opioids in luteinizing hormone (LH) secretion by pituitary cells of common carp, Cyprinus carpio (Actinopterygii: Cypriniformes: Cyprinidae), cultured in vitro

2011 ◽  
Vol 41 (2) ◽  
pp. 81-87
Author(s):  
Mirosława Sokołowska-Mikołajczyk ◽  
Magdalena Socha ◽  
Tomasz Mikołajczyk ◽  
Piotr Epler ◽  
Barbara Fałowska
Keyword(s):  
Luteinizing Hormone ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Pituitary Cells ◽  
Lh Secretion

scholarly journals Sex dependent action of Aroclor 1254 on basal and sGnRHa-stimulated secretion of LH from the pituitary cells of common carp, Cyprinus carpio L.

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Magdalena Socha ◽  
Mirosława Sokołowska-Mikołajczyk ◽  
Jarosław Chyb ◽  
Ewa Drąg-Kozak ◽  
Ewa Łuszczek-Trojnar
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Hormonal Regulation ◽  
Pituitary Cells ◽  
Aroclor 1254 ◽  
Cell Incubation ◽  
The Media ◽  
Lh Release ◽  
Sites Of Action ◽  
Lh Secretion

AbstractPolychlorinated biphenyls (PCBs) affect the hypothalamic-pituitary-gonadal axis in many vertebrates, changing the hormonal regulation of reproduction. To identify one of the possible sites of action of PCBs on gonadotropin release in common carp, the direct effects of Aroclor 1254 on luteinizing hormone (LH) secretion from dispersed pituitary cells were investigated. Pituitary cells were obtained from sexually mature male and female common carp (Cyprinus carpio L.) at the time of natural spawning. The cells were incubated with different concentrations of Aroclor 1254 (5, 10, 50 and 100 ng mL−1 medium) and/or salmon gonadotropin-releasing hormone analogue (sGnRHa) at a concentration of 10−8 M. LH levels were measured in the cultured medium by the ELISA method after 10 hours of cell incubation. Incubation of male pituitary cells in the presence of tested concentrations of Aroclor did not change the basal LH secretion to the media. In the female pituitary cell incubations Aroclor (5, 10 and 100 ng mL−1 medium) caused a significant increase in LH concentrations in comparison to control incubations. In the case of sGnRHa-stimulated LH secretion in incubations of cells of both sexes, all the concentrations of Aroclor significantly stimulated LH release and potentiated stimulatory effects of sGnRH analogue. These results indicate that endocrine disrupters, such as Aroclor 1254, may affect reproduction in fish, acting also directly on gonadotrophs at the level of the pituitary gland, changing LH secretion.

scholarly journals  Steroid effects on the in vitro LH secretion from pituitary cells of sexually immature carp (Cyprinus carpio L.) under the influence of naltrexone and morphine

2010 ◽  
Vol 55 (No. 6) ◽  
pp. 250-257
Author(s):  
M. Sokolowska-Mikolajczyk ◽  
M. Socha ◽  
P. Epler
Keyword(s):  
Cyprinus Carpio ◽  
Sex Steroids ◽  
Pituitary Cells ◽  
Control Medium ◽  
Opioid Agonist ◽  
Carp Cyprinus Carpio ◽  
Lh Release ◽  
Lh Secretion

Dispersed cells from sexually immature carp (footlings) pituitaries were exposed to estradiol (E<sub>2</sub>) or testosterone (T) (both at 3 &times; 10<sup>&ndash;8</sup> M) in the presence of opioid receptor antagonist naltrexone (10<sup>&ndash;8</sup> or 10<sup>&ndash;6</sup> M) and/or agonist &ndash; morphine (10<sup>&ndash;8</sup> or 10<sup>&ndash;6</sup> M). Naltrexone alone at 10<sup>&ndash;6</sup> M increased the LH level as compared with the control. Morphine (10<sup>&ndash;8</sup> or 10<sup>&ndash;6</sup> M), T and E2 had no influence on LH levels. The combination of T with naltrexone (10<sup>&ndash;8</sup> M) stimulated LH release if compared with the control or with T alone. Morphine (both concentrations) with T caused significantly higher LH secretion than the control medium and T alone. Estradiol with naltrexone (10<sup>&ndash;8</sup> and 10<sup>&ndash;6</sup> M) had no influence on LH concentration. In media with E2 and morphine (10<sup>&ndash;8</sup> M) LH levels were higher than in the control and estradiol alone. The results show that in common carp sex steroids affect the response of pituitary cells to opioid agonist or antagonist giving an evidence on the role of steroids in LH release mediated by the opioid system.

Energetic dependence of NPY-induced LH secretion in a teleost fish (Dicentrarchus labrax)

1999 ◽  
Vol 277 (6) ◽  
pp. R1627-R1634 ◽  
Author(s):  
José Miguel Cerdá-Reverter ◽  
Lisa Ann Sorbera ◽  
Manuel Carrillo ◽  
Silvia Zanuy
Keyword(s):  
Glucose Metabolism ◽  
Luteinizing Hormone ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Pituitary Cells ◽  
Lh Secretion ◽  
Dose Dependent Increase ◽  
Dose Dependent

The purpose of this work was to examine the role of energetic status in neuropeptide Y (NPY)-induced luteinizing hormone (LH) secretion and glucose metabolism in fish. Fasted juvenile sea bass ( Dicentrarchus labrax) were injected intraperitoneally with pig (p) NPY or pNPY + glucose, whereas fed animals were injected with pNPY alone and plasma glucose, insulin, and LH levels were examined. pNPY alone or in combination with glucose was found to induce a dose-dependent increase in LH secretion in fasted animals. Similar LH responses to pNPY were observed in vitro in dispersed pituitary cells isolated from fed and fasted animals incubated in L-15 and restricted media. Injection of pNPY + glucose in fasted animals resulted in depletion of glucose. Insulin plasma levels decreased in fasted animals coinjected with pNPY + glucose but remained stable when NPY was administrated alone to fed and fasted animals. Results suggest that 1) NPY-induced LH secretion in fish is dependent on energetic status and 2) NPY is capable of modifying glucose metabolism.

scholarly journals In vitro cultivation of Trypanoplasma borreli (Protozoa:Kinetoplastida), a parasite from the blood of common carp Cyprinus carpio

2000 ◽  
Vol 41 ◽  
pp. 195-201 ◽  
Author(s):  
D Steinhagen ◽  
W Hedderich ◽  
A Skouras ◽  
JP Scharsack ◽  
J Schuberth ◽  
...  
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
In Vitro Cultivation ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio

scholarly journals The in vitro toxicity of atrazine on kinematics and DNA fragmentation in common carp (Cyprinus carpio) sperm cells

2019 ◽  
Vol 70 (3) ◽  
pp. 1639
Author(s):  
M.E. ÖZGÜR
Keyword(s):  
Dna Fragmentation ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
In Vitro Toxicity ◽  
Computer Assisted ◽  
Sperm Cells ◽  
Common Carp Cyprinus Carpio ◽  
Significant Difference ◽  
Carp Cyprinus Carpio

This study investigated the in vitro effects of different concentrations of Atrazine (0.001, 0.01, 0.1, 0.5 mg/L) added to motile and immotile solutions on kinematics quality of sperm cells of common carp, Cyprinus carpio, which is a fish of economic significance. The kinematics of the sperm cells was analyzed by a computer-assisted sperm analysis system (CASA). As a result of the study, while there was a significant difference (P < 0.05) between the groups in terms of the VSL (μm/s) and VCL (μm/s) values after the Atrazine-added immotile solution’s (IMS) and incubation for 3 hours, there was a significant difference (P < 0.05) in only the VSL values directly activated by the Atrazine-added motile solution (MS). DNA fragmentation was evident but not in higher numbers in the 0.1 mg/L atrazine group. Finally, it was determined the effective concentration (EC50) values of the VSL value of the motile and immotile solution as 0.34 mg/L and 0.03 mg/L, respectively.

Ionophore A23187 partially reverses LH secretory defect of pituitary cells from old rats

1987 ◽  
Vol 253 (3) ◽  
pp. E233-E237
Author(s):  
R. S. Chuknyiska ◽  
M. R. Blackman ◽  
G. S. Roth
Keyword(s):  
Primary Cultures ◽  
Extracellular Calcium ◽  
Base Line ◽  
Ionophore A23187 ◽  
Pituitary Cells ◽  
Lh Release ◽  
Old Rats ◽  
Lh Releasing Hormone ◽  
Lh Secretion

We measured in vitro release of luteinizing hormone (LH) in the presence of 1.5 mM extracellular calcium, with and without LH-releasing hormone (LHRH; 10(-10) to 10(-7) M) or the ionophore A23187 (10(-7) to 10(-4) M), in primary cultures of anterior pituitary cells from intact mature (6 mo) and old (24 mo) male and intact and ovariectomized mature and old female Wistar rats. Base-line as well as LHRH- and A23187-mediated LH secretion was decreased from cells of old rats. However, exposure to A23187 led to a nearly twofold greater augmentation of LH release from cells of old rats, thus decreasing the apparent age-related LH secretory deficit by approximately one-half. We then measured LHRH-mediated (10(-8) M) vs. A23187-mediated (10(-4) M) LH release with and without extracellular calcium (0.08-1.5 mM). For cells from both mature and old rats, there was a similar calcium dependency for A23187- and LHRH-mediated LH release, with optimal LH secretion at 1.0-1.5 mM extracellular calcium concentrations. Again, both LHRH- and A23187-stimulated LH release was significantly lower and exposure to A23187 led to a greater increase in LH release from cells of old rats. Taken together with similar findings in other systems, these data suggest that the in vitro LH secretory defect of pituitary cells from old rats results in part from one or more defects in calcium mobilization and that such alterations may be a widespread manifestation of aging.

Stimulatory effect of thyrotrophin-releasing hormone (TRH) on the release of luteinizing hormone (LH) from rat anterior pituitary cells in vitro

1983 ◽  
Vol 61 (2) ◽  
pp. 186-189 ◽  
Author(s):  
Noboru Fujihara ◽  
Masataka Shiino
Keyword(s):  
Luteinizing Hormone ◽  
Anterior Pituitary ◽  
Pituitary Cells ◽  
Thyrotrophin Releasing Hormone ◽  
Releasing Hormone ◽  
Anterior Pituitary Cells ◽  
Lh Release ◽  
Lh Rh

The effect of thyrotrophin-releasing hormone (TRH, 10−7 M) on luteinizing hormone (LH) release from rat anterior pituitary cells was examined using organ and primary cell culture. The addition of TRH to the culture medium resulted in a slightly enhanced release of LH from the cultured pituitary tissues. However, the amount of LH release stimulated by TRH was not greater than that produced by luteinizing hormone – releasing hormone (LH–RH, 10−7 M). Actinomycin D (2 × 10−5 M) and cycloheximide (10−4 M) had an inhibitory effect on the action of TRH on LH release. The inability of TRH to elicit gonadotrophin release from the anterior pituitary glands in vivo may partly be due to physiological inhibition of its action by other hypothalamic factor(s).

Oxytocin modulates the luteinizing hormone response of the rat anterior pituitary to gonadotrophin-releasing hormone in vitro

1995 ◽  
Vol 145 (1) ◽  
pp. 113-119 ◽  
Author(s):  
J J Evans ◽  
S J Hurd ◽  
D R Mason
Keyword(s):  
Luteinizing Hormone ◽  
Anterior Pituitary ◽  
The Self ◽  
Female Rats ◽  
Hormone Response ◽  
Priming Process ◽  
Lh Release ◽  
Gonadotrophin Releasing Hormone ◽  
Lh Secretion

Abstract Although GnRH is believed to be the primary secretagogue for LH, oxytocin has also been shown to stimulate LH release from the anterior pituitary. We investigated the possibility that the two secretagogues interact in the modulation of LH release. Anterior pituitaries were removed from adult female rats at pro-oestrus, and tissue pieces were pre-incubated in oxytocin for 3 h prior to being stimulated with 15 min pulses of GnRH. LH output over the 1 h period from the beginning of the GnRH pulse was determined. Control incubations were carried out in the absence of oxytocin, and background secretory activities without GnRH stimulation were also determined. Tissue which was pre-exposed to oxytocin (0·012–1·25 μm) had an increased LH response to GnRH (1·25 nm). The increase was larger than the sum of the LH outputs obtained with oxytocin and GnRH separately, revealing that oxytocin synergistically enhanced LH secretion elicited by GnRH (P<0·05; ANOVA). If stimulation by GnRH was delayed for 2 h after incubation with oxytocin, an increase in LH secretion was still observed, indicating that oxytocin-induced modulation did not rapidly disappear. Oxytocin pre-incubation was observed to result in an increase of maximal GnRH-induced LH output (P<0·001; t-test), as well as an increase of intermediate responses. The LH response of the anterior pituitary to subsequent pulses of GnRH was modified by the self-priming process. The effect of oxytocin pretreatment on the response of primed tissue to GnRH was also investigated. It was found that pre-incubation in oxytocin also enhanced the LH response of primed tissue to GnRH. The study has revealed that oxytocin increases the LH output of anterior pituitary tissue in response to GnRH. The effect occurs on both GnRH-primed and unprimed tissues. The results suggest that oxytocin has the potential to regulate the dynamics of the pro-oestrous LH surge. Journal of Endocrinology (1995) 145, 113–119

Sign in / Sign up

Export Citation Format

Share Document