Change of aquaporin-1 in rat models of kidney-yang-deficiency syndrome

2008 ◽  
Vol 6 (5) ◽  
pp. 498-501 ◽  
Author(s):  
Y Li
Keyword(s):  
Deficiency Syndrome ◽  
Rat Models ◽  
Aquaporin 1 ◽  
Kidney Yang Deficiency

scholarly journals Establishment and Comparison of Combining Disease and Syndrome Model of Asthma with “Kidney Yang Deficiency” and “Abnormal Savda”

2013 ◽  
Vol 2013 ◽  
pp. 1-15 ◽  
Author(s):  
Bei Li ◽  
Qing-li Luo ◽  
Mammat Nurahmat ◽  
Hua-liang Jin ◽  
Yi-jie Du ◽  
...  
Keyword(s):  
Airway Remodeling ◽  
Deficiency Syndrome ◽  
High Dose ◽  
Rat Models ◽  
Traditional Uighur Medicine ◽  
Kidney Yang Deficiency ◽  
Abnormal Savda ◽  
Abnormal Savda Syndrome ◽  
Nerve System ◽  
First Time

The study was the first time to establish and compare two rat models of two common syndromes: Kidney Yang Deficiency syndrome (KYDS) in traditional Chinese medicine (TCM) and abnormal savda syndrome (ASS) in traditional Uighur medicine (TUM). Then, we also established and evaluated rat models of combining disease and syndrome models of asthma with KYDS or ASS. Results showed that usage of the high dose of corticosterone (CORT) injection or external factors could successfully establish the KYDS or ASS rat models, and the two models had similar changes in biological characterization, abnormal behaviors, dysfunction of hypothalamic-pituitary-target organ axes (HPTOA), and sympathetic/parasympathetic (S/P) nerve system but varied in different degrees. The rat models of combining disease and syndrome of asthma with KYDS or ASS had either pathological characteristics of asthma such as airway hyperresponsiveness (AHR), airway inflammation, airway remodeling, which were more serious than allergy exposure alone, or the syndrome performance of Kidney Yang Deficiency in TCM and abnormal savda in TUM. These findings provide a biological rationale for further investigation of combining disease and syndrome model of asthma as an effective animal model for exploring asthma based on the theory of traditional medicine.

scholarly journals Effects of Haima Duobian Pill in a Rat Model of Kidney Yang Deficiency Syndrome

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Yijia Zeng ◽  
Tingna Li ◽  
Xiaorui Zhang ◽  
Yuanyuan Ren ◽  
Qinwan Huang ◽  
...  
Keyword(s):  
Rat Model ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Cyclic Guanosine Monophosphate ◽  
Deficiency Syndrome ◽  
Guanosine Monophosphate ◽  
Network Pharmacology ◽  
Enzyme Linked Immunosorbent Assay ◽  
Therapeutic Effects ◽  
Kidney Yang Deficiency

Objective. Modern research shows that Haima Duobian pill (HDP) can relieve the kidney yang deficiency syndrome (KYDS), but the mechanism is still unclear. The aim of this work was to study the effects of HDP in a rat model of KYDS. Materials and Methods. The network pharmacology methods were used to predict the therapeutic effects of Haima Duobian pill. Adenine was used to establish the rat model of kidney yang deficiency syndrome. The general physical signs of rats were observed after different doses of Haima Duobian pill (HDP) were given. Serum cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP), luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone (T), estradiol (E2), and gonadotropin-releasing hormone (GnRH) levels were determined using enzyme-linked immunosorbent assay (ELISA) kits. Then, the histopathologic changes and sperm activity were detected. Results. HDP could improve the general signs of kidney yang deficiency syndrome rats. After the rats were treated with HDP, the expression of cGMP and E2 was significantly inhibited and the expression of cAMP and T was significantly increased. The pathological damage of testis, epididymis, and seminal vesicle was alleviated, and the sperm activity was improved. Conclusion. For adenine-induced kidney yang deficiency syndrome in rats, HDP had a significant therapeutic effect.

scholarly journals Resequencing DCDC5 in the Flanking Region of an LD-SNP Derived from a Kidney-Yang Deficiency Syndrome Family

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Li Ping Zhou ◽  
Wei Wei Liu ◽  
Tian E. Zhang ◽  
Wei Hong Li ◽  
Ling Ling Tan ◽  
...  
Keyword(s):  
Dna Analysis ◽  
Substitution Effect ◽  
Deficiency Syndrome ◽  
Trna Synthetase ◽  
Genomic Variation ◽  
Single Nucleotide ◽  
Genetic Traits ◽  
Kidney Yang Deficiency ◽  
Flanking Region ◽  
Nonsynonymous Variation

Objective. To explore the genetic traits of Kidney-yang deficiency syndrome (KDS).Design. Twelve KDS subjects and three spouses from a typical KDS family were recruited. Their genomic DNA samples were genotyped by Affymetrix 100K single-nucleotide polymorphism (SNP) arrays. The linkage disequilibrium (LD) SNPs were generated using GeneChip DNA analysis software (GDAS, Affymetrix). Genes located within 100 bp of the flanks of LD SNPs were mined via GeneView. 29 exons of the doublecortin domain containing 5 (DCDC5), a representative gene within the flank of an LD SNP, were resequenced.Results. Five LD SNPs display midrange linkage with KDS. Two genes with established functions, DCDC5 and Leucyl-tRNA synthetase, were mined in the flanks of LD SNPs. Resequencing of DCDC5 revealed a nonsynonymous variation, in which 3764T/A was replaced by C/G. Accordingly, the Ser1172was substituted by Pro1172. The S1172P substitution effect was evaluated as “possibly damaging” by PolyPhen.Conclusion. We have identified a genomic variation of DCDC5 based on the LD SNPs derived from a KDS family. DCDC5 and other genes surrounding these SNPs display some relationships with key symptoms of KDS.

Mahuang-Xixin-Fuzi decoction reduces the infection of influenza A virus in Kidney-Yang deficiency syndrome mice

2016 ◽  
Vol 192 ◽  
pp. 217-224 ◽  
Author(s):  
Rong Rong ◽  
Rong-rong Li ◽  
Yan-bao Hou ◽  
Jing Li ◽  
Jia-xing Ding ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Deficiency Syndrome ◽  
Kidney Yang Deficiency

Structural–Activity Relationship of Ginsenosides from Steamed Ginseng in the Treatment of Erectile Dysfunction

2018 ◽  
Vol 46 (01) ◽  
pp. 137-155 ◽  
Author(s):  
Ang Ying ◽  
Qing-Tao Yu ◽  
Li Guo ◽  
Wen-Song Zhang ◽  
Jin-Feng Liu ◽  
...  
Keyword(s):  
Erectile Dysfunction ◽  
Hypoxia Inducible Factor ◽  
Corpus Cavernosum ◽  
Cyclic Guanosine Monophosphate ◽  
Deficiency Syndrome ◽  
Guanosine Monophosphate ◽  
Hydroxyl Groups ◽  
Mice Model ◽  
Kidney Yang Deficiency ◽  
Relationship Of

Ginseng has been reported to have diverse pharmacological effects. One of the therapeutic claims for ginseng is to enhance sexual function. Ginsenosides are considered as the major active constituents. A steaming process can alter the ginsenoside profile of ginseng products. The structure–function relationship of ginsenosides in the treatment of erectile dysfunction (ED) has not been investigated yet. In this work, 15 different processed ginsengs are produced by steaming, and 13 major ginsensosides are quantified by liquid chromatography with UV detection, including Rg1, Re, Rf, Rb1, Rc, Rb2, Rf, Rk3, Rh4, 20S-Rg3, 20R-Rg3, Rk1, and Rg5. Their anti-ED activities are screened using hydrocortisone-induced mice model (Kidney Yang Deficiency Syndrome in Chinese Medicine) and primary corpus cavernosum smooth muscle cells (CCSMCs). A processed ginseng with steaming treatment at 120[Formula: see text]C for 4[Formula: see text]h and five times possesses abundant ginsenosides Rk1, Rk3, Rh4 and Rg5 transformed via deglycosylation and dehydroxylation, and produces optimal activity against ED. The number of sugar molecules, structure of hydroxyl groups and stereoselectivity in ginsenosides affect their anti-ED activity. Among the 13 ginsenosides, Rk1, Rk3, Rh4 and Rg5 are the most efficient in decreasing intracellular calcium levels by inhibiting phosphodiesterase 5A (PDE5A) to reduce the degradation of cyclic guanosine monophosphate (cGMP) in CCSMCs. Rg5 also restrain hypoxia inducible factor-1[Formula: see text] (HIF-1[Formula: see text] expression in hypoxia state, and increase endothelial nitric oxide synthase (eNOS) expression in isolated rat cavernous tissue. These observations suggest a role for steamed ginseng containing two pairs of geometric isomers (i.e., Rk1/Rg5 and Rk3/Rh4) in the treatment of ED.

scholarly journals The Th17/Treg Immune Balance in Ulcerative Colitis Patients with Two Different Chinese Syndromes: Dampness-Heat in Large Intestine and Spleen and Kidney Yang Deficiency Syndrome

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Yang Gong ◽  
Lixing Liu ◽  
Xiaojuan He ◽  
Hongyan Zhao ◽  
Jing Yang ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Flow Cytometry ◽  
Large Intestine ◽  
Colonic Mucosa ◽  
Deficiency Syndrome ◽  
Healthy Control Group ◽  
Control Group ◽  
Immune Balance ◽  
Kidney Yang Deficiency ◽  
Healthy Control

Objective. To investigate the Th17/Treg immune balance in the ulcerative colitis (UC) patients with two Chinese syndrome: dampness-heat in large intestine (DHLI) and spleen and kidney Yang deficiency (SKYD).Methods. Ninety UC patients (45 were diagnosed with DHLI and 45 with SKYD syndrome) and 23 healthy people were recruited. The serumIL-17 and TGF-β1 levels of these participants were measured with ELISA; the expression of IL-17 and TGF-β1 in colonic mucosa tissue was determined with immunohistochemistry and the percentage of Th17 and Treg in peripheral blood with flow cytometry.Results. The levels of IL-17 and Th17 were significantly higher in both DHLI and SKYD groups than in healthy control group and higher in DHLI than in SKYD group (P<0.05). The levels of TGF-β1 and Treg were significantly lower in the two UC patients groups than in healthy control group; and lower in SKYD group than in DHLI group (P<0.05).Conclusions. UC with DHLI syndrome could be characterized by the elevation of Th17 and IL-17 levels, which indicated an accentuation of inflammatory reaction; UC with SKYD syndrome could be characterized by the reduction of serum Treg and TGF-β1 levels, which represented a depression of immune tolerance.

scholarly journals Chronic hypertension increases aortic endothelial hydraulic conductivity by upregulating endothelial aquaporin-1 expression

2017 ◽  
Vol 313 (5) ◽  
pp. H1063-H1073 ◽  
Author(s):  
Jimmy Toussaint ◽  
Chirag Bharavi Raval ◽  
Tieuvi Nguyen ◽  
Hadi Fadaifard ◽  
Shripad Joshi ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Hydraulic Conductivity ◽  
Water Transport ◽  
Aortic Endothelial Cells ◽  
Rat Models ◽  
Aquaporin 1 ◽  
Hypertensive Rat ◽  
And Function ◽  
Aortic Endothelial

Numerous studies have examined the role of aquaporins in osmotic water transport in various systems, but virtually none have focused on the role of aquaporin in hydrostatically driven water transport involving mammalian cells save for our laboratory’s recent study of aortic endothelial cells. Here, we investigated aquaporin-1 expression and function in the aortic endothelium in two high-renin rat models of hypertension, the spontaneously hypertensive genetically altered Wistar-Kyoto rat variant and Sprague-Dawley rats made hypertensive by two-kidney, one-clip Goldblatt surgery. We measured aquaporin-1 expression in aortic endothelial cells from whole rat aortas by quantitative immunohistochemistry and function by measuring the pressure-driven hydraulic conductivities of excised rat aortas with both intact and denuded endothelia on the same vessel. We used them to calculate the effective intimal hydraulic conductivity, which is a combination of endothelial and subendothelial components. We observed well-correlated enhancements in aquaporin-1 expression and function in both hypertensive rat models as well as in aortas from normotensive rats whose expression was upregulated by 2 h of forskolin treatment. Upregulated aquaporin-1 expression and function may be a response to hypertension that critically determines conduit artery vessel wall viability and long-term susceptibility to atherosclerosis. NEW & NOTEWORTHY The aortic endothelia of two high-renin hypertensive rat models express greater than two times the aquaporin-1 and, at low pressures, have greater than two times the endothelial hydraulic conductivity of normotensive rats. Data are consistent with theory predicting that higher endothelial aquaporin-1 expression raises the critical pressure for subendothelial intima compression and for artery wall hydraulic conductivity to drop.

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