scholarly journals Hybrid Beads Bearing Immobilized Bacteria as Advanced Means for the Removal of Acid Blue 93 Dye

2020 ◽  
Vol 57 (3) ◽  
pp. 125-136
Author(s):  
Teodor Sandu ◽  
Maria Luiza Jecu ◽  
Iuliana Raut ◽  
Mariana Calin ◽  
Elvira Alexandrescu ◽  
...  
Keyword(s):  
Uv Spectroscopy ◽  
Blue Dye ◽  
Pseudomonas Sp ◽  
Sem Images ◽  
Magnetite Fe3o4 ◽  
Hybrid Copolymer ◽  
Before And After ◽  
Wet Phase Inversion ◽  
Poly Acrylonitrile ◽  
Acid Blue

This paper describes the preparation of three- component hybrid copolymer beads, with water purification features. These newly developed hybrid beads were prepared starting from a mixture of poly(acrylonitrile- co- methacrylic acid) (PAN-co-PMAA), polyvinyl alcohol (PVA) and magnetite (Fe3O4), respectively. The preparation itself involved a Wet Phase Inversion (WPI) process. A Pseudomonas sp. strain was immobilized onto previously mentioned beads, before and after activation of the beads surface with glutardialdehyde, and afterwards used for the amendment of simulated water bearing an azo- blue dye, i.e. Acid Blue 93. In order to highlight the immobilization of Pseudomonas sp. strain, FTIR spectra and TGA results were recorded. CFU measurements as well as SEM images further provided evidence towards the occurrence of immobilization. The biodegradation studies of Acid Blue 93were carried out by means of UV spectroscopy at various contact times (24; 72 and 144 h) of the hybrid beads with the targeted dye.

scholarly journals Toxicity and Biological Effects of Beauveria brongniartii Fe0 Nanoparticles against Spodoptera litura (Fabricius)

Insects ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 895
Author(s):  
Jing Xu ◽  
Kaihui Zhang ◽  
Andrew G. S. Cuthbertson ◽  
Cailian Du ◽  
Shaukat Ali
Keyword(s):  
Spodoptera Litura ◽  
Insect Pests ◽  
Growth Parameters ◽  
Uv Spectroscopy ◽  
Biological Effects ◽  
Fungal Treatment ◽  
Beauveria Brongniartii ◽  
Sem Images ◽  
X Ray ◽  
Infection Period

Nanotechnology has clear potential in the development of innovative insecticidal products for the biorational management of major insect pests. Metal-based nanoparticles of different microbial pest control agents have been effective against several pests. Synthesis of Beauveria brongniartii based Fe0 nanoparticles (Fe0NPs) and their bio-efficacy against Spodoptera litura was observed during this study. Beauveria brongniartii conidia were coated with Fe0NPs and characterized by applying a selection of different analytical techniques. Ultraviolet (UV) spectroscopy showed the characteristic band of surface plasmon at 430 nm; Scanning electron microscopy (SEM) images showed spherical shaped nanoparticles with a size ranging between 0.41 to 0.80 µm; Energy-dispersive X-ray (EDX) spectral analysis revealed characteristic Fe peaks at 6.5 and 7.1 Kev; the X-ray diffractogram showed three strong peaks at 2θ values of 45.72°, 64.47°, and 84.05°. The bioassay studies demonstrated that mortality of 2nd instar S. litura larvae following Fe0NPs treatment increased with increasing concentrations of Fe0NPs at different time intervals. The median lethal concentration (LC50) values of Fe0NPs against S. litura after seven days of fungal treatment was 59 ppm, whereas median survival time (LT50) values for 200 and 500 ppm concentrations of Fe0NPs against S. litura seven days post-treatment were 5.1 and 2.29 days, respectively. Beauveria brongniartii-Fe0NPs caused significant reductions in feeding and growth parameters (relative growth rate, relative consumption rate, and efficiency of conversion of ingested food) of S. litura. Beauveria brongniartii Fe0NPs induced reduction in glutathione-S-transferase activities throughout the infection period whereas activities of antioxidant enzymes decreased during later periods of infection. These findings suggest that B. brongniartii Fe0NPs can potentially be used in biorational S. litura management programs.

Removal of methylene blue dye from aqueous medium by nano poly acrylonitrile particles

2012 ◽  
Vol 44 (1-3) ◽  
pp. 151-160 ◽  
Author(s):  
Mohamed Samir Mohy Eldin ◽  
S.A. El-Sakka ◽  
M.M. El-Masry ◽  
I.I. Abdel-Gawad ◽  
S.S. Garybe
Keyword(s):  
Methylene Blue ◽  
Aqueous Medium ◽  
Blue Dye ◽  
Methylene Blue Dye ◽  
Poly Acrylonitrile

scholarly journals Research on Mechanical Performance & Behaviour of Geopolymer Concrete Subjected to Elevated Temperatures

2019 ◽  
Vol 8 (2S8) ◽  
pp. 883-887
Keyword(s):  
Compressive Strength ◽  
Fly Ash ◽  
Silica Fume ◽  
Elevated Temperatures ◽  
Mechanical Performance ◽  
Geopolymer Concrete ◽  
Sem Images ◽  
Before And After ◽  
Alkaline Conditions ◽  
Compressive Strength Of Concrete

The investigative studies on mechanical performance & behaviour, of Geopolymer Concrete (GPC) before and after the exposure to elevated temperatures (of 200 0 C -1000 0 C with an increment of 100 0 C). Indicate that the GPC Specimens Exhibited better Compressive strength at higher temperatures than that of those made by regular OPC Concrete with M30 Grade. The chronological changes in the geopolymeric structure upon exposure to these temperatures and their reflections on the thermal behaviour have also been explored. The SEM images indicate GPC produced by fly ash , metakaolin and silica fume, under alkaline conditions form Mineral binders that are not only non-flammable and but are also non-combustible resins and binders. Further the Observations drawn disclose that the mass and compressive strength of concrete gets reduced with increase in temperatures.

Study on Adsorption Properties of Different Dyes on Montmorillonite

2012 ◽  
Vol 454 ◽  
pp. 305-309 ◽  
Author(s):  
Ping Chen ◽  
Xian Jun Lu ◽  
Jun Qiu
Keyword(s):  
Methylene Blue ◽  
Ph Value ◽  
Carbon Chain ◽  
Adsorption Properties ◽  
Organically Modified ◽  
Optimum Ph ◽  
Before And After ◽  
Higher Temperature ◽  
Adsorption Amount ◽  
Acid Blue

Using Octadecyltrimethylammonium cations with long carbon chain to modify calcium montmorillonite samples, the adsorption properties of different dyes on montmorillonite before and after modification were investigated. Results showed that the basic dye of methylene blue was easily adsorbed by natual calcium montmorillonite, while the adsorption ability for acid blue and neutral blue was greatly improved when it was organically modified, and the adsorption amount increased with the increasing of modifier dosage. The adsorption ratio was influnced by reaction time, the temperature and the solution’s pH value. It suggested that the adsorption balance time was about 60min; higher temperature was good for acid blue adsorbed on organic montmorillonite, but neutral blue’s adsorption amount was greater at the room temperature; the optimum pH was 9 for methylene blue adsorption, while low pH was better for acid blue and neutral blue adsorption.

Utilization of eutrophicated Lemna minor for biosorption of acid blue dye

2021 ◽  
Author(s):  
P. Prakash ◽  
J. Aravind Kumar ◽  
Balaji Dhandapani ◽  
Dhanya Vishnu ◽  
S. Hrishitha Sree ◽  
...  
Keyword(s):  
Lemna Minor ◽  
Blue Dye ◽  
Acid Blue

Transplantation of Retinal Pigment Epithelium Using Viable Cryopreserved Cells

1997 ◽  
Vol 6 (2) ◽  
pp. 149-162 ◽  
Author(s):  
Yusuf K. Durlu ◽  
Makoto Tamai
Keyword(s):  
Tissue Culture ◽  
Indirect Elisa ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Light And Electron Microscopy ◽  
Blue Dye ◽  
Rpe Cells ◽  
Before And After ◽  
Rcs Rats ◽  
Human Rpe Cells

Transplantation of retinal pigment epithelium (RPE) may have potential clinical application for the surgical treatment of RPE-specific retinal degeneration, including age-related macular degeneration. The feasibility of an RPE storage bank has been investigated by experimenting with transplantation using viable, cryopreserved RPE cells. Fresh and cultured fetal human and bovine RPE cells were cryopreserved in 90% fetal bovine serum containing 10% dimethyl sulfoxide. The viability of the cells before and after cryopreservation was evaluated by trypan blue dye exclusion test, microculture tetrazolium assay (MTA), tissue culture, and transplantation after cryopreservation. The origin of RPE cells before and after cryopreservation was assessed by immunocytochemistry, immunoblotting, and indirect ELISA of RPE-marker protein using cytokeratin for cultured fetal human RPE cells and by immunocytochemistry of cellular retinaldehyde-binding protein (CR-ALBP) for cultured bovine RPE cells. Freshly isolated and cryopreserved uncultured bovine RPE cells were transplanted by posterior transscleral approach into the subretinal spaces of adult albino rabbits and 23-day-old Royal College of Surgeons (RCS) rats with a 33 gauge Hamilton syringe. Following surgery, artificial retinal blebs were confirmed by fundus examination. Morphologic examination was performed postoperatively by light and electron microscopy in albino rabbits and by light microscopy in RCS rats up to 3 mo. Control subretinal injections using vehicle solution also were performed in RCS rats. Cultured fetal human and bovine RPE cells after cryopreservation were found to be viable, based on the results of trypan blue dye exclusion test, MTA, tissue culture, and transplantation. Expression and reexpression of cytokeratin intermediate filaments in cultured fetal human RPE were demonstrated by immunocytochemistry, immunoblotting, and indirect ELISA before and after cryopreservation. Immunocytochemistry of CRALBP before and after cryopreservation in uncultured bovine RPE cells disclosed expression and reexpression of RPE cell marker protein. No uncultured fetal human RPE cells showed proliferation in tissue culture after cryopreservation. In rabbits, light and electron microscopy disclosed xenografted RPE cells residing on Bruch's membrane of the host retina. No sign of graft vs. host reaction was observed. No morphologic difference was noted between the fresh and 10-day-old cryopreserved RPE cells in situ following transplantation at day 25. In RCS rats, subretinal injection of 3-wk-old cryopreserved bovine RPE cells partially rescued photoreceptor cells locally at the transplanted area observed at 3 mo postoperatively. The retinal photoreceptors at the inferior hemisphere of the transplanted eye and the eye injected with vehicle solution showed no rescue effect. We found that cryopreserved cultured fetal human RPE cells and uncultured and cultured bovine RPE cells can be used for RPE transplantation studies. The ability to create an RPE storage bank as a source of donor cells may result in several clinical advantages.

scholarly journals Biodecolorization of anthraquinone dyes using immobilised mycelium of Bjerkandera adusta CCBAS930

2020 ◽  
Vol 171 ◽  
pp. 01013 ◽  
Author(s):  
Kamila Rybczyńska-Tkaczyk ◽  
Teresa Korniłłowicz-Kowalska
Keyword(s):  
Phenolic Compounds ◽  
Lepidium Sativum ◽  
Carminic Acid ◽  
Secondary Products ◽  
Bjerkandera Adusta ◽  
Anthraquinone Dyes ◽  
Before And After ◽  
Acid Blue 129 ◽  
Acid Blue ◽  
Acid Green 25

The aim of this study was to characterize the activity of oxidoreductases during biotransformation of 0.01% anthraquinone dyes: Alizarin Blue Black B (ABBB) and Acid Blue 129 (AB129), Carminic Acid (CA), Remazol Brilliant Blue R (RBBR), Acid Green 25 (AG25) and Poly R-478 by immobilized strain of Bjerkandera adusta CCBAS 930. Phenolic compounds, phytotoxicity (Lepidium sativum L.), biotoxicity were evaluated to determine the toxicity of anthraquinone dyes before and after the treatment with immobilized B. adusta CCBAS 930. More than 60% of CA and AB129 were removed by immobilized B. adusta CCBAS after 7 days. No secondary products toxic to plants and bacteria were formed during immobilized cultures of B. adusta CCBAS 930.

Photocatalytic Comparative Study of TiO2, ZnO, Ag-G-ZnO and Ag-G-TiO2 Nanocomposite Films

2017 ◽  
Vol 751 ◽  
pp. 825-830 ◽  
Author(s):  
Phuri Kalnaowakul ◽  
Tonghathai Phairatana ◽  
Aphichart Rodchanarowan
Keyword(s):  
Colloidal Solution ◽  
Nanocomposite Films ◽  
Blue Dye ◽  
Methylene Blue Dye ◽  
X Ray Diffraction ◽  
Sem Images ◽  
X Ray ◽  
Vis Spectroscopy ◽  
Photocatalytic Activities ◽  
Xrd Patterns

In this study, the photocatalytic properties and morphology of TiO2, ZnO, Ag-graphene-zinc oxide (Ag-G-ZnO) and Ag-graphene-titanium dioxide (Ag-G-TiO2) nanocomposite were compared. The Ag-G-ZnO and Ag-G-TiO2 nanocomposite were successfully prepared by thermal decomposition of colloidal solution. These prepared composites were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-Vis spectroscopy and photocatalytic activities. The results from XRD patterns show that Ag-G-TiO2 composites and the Ag-G-ZnO nanocomposites were in the form of fcc and hcp crystal structure, respectively. The SEM images show that at calcination of 500 °C for 3 h, the composite thin film of Ag-G-ZnO and Ag-G-TiO2 were homogenous. In the case of the photocatalytic experiments using methylene blue dye (MB) under UV irradiation, the order of the photocatalytic activities from high to low performances are Ag-G-ZnO, Ag-G-TiO2, ZnO and TiO2, respectively.

Macroscale Mechanical and Microscale Structural Changes in Chinese Wufeng Shale With Supercritical Carbon Dioxide Fracturing

SPE Journal ◽  
2017 ◽  
Vol 23 (03) ◽  
pp. 691-703 ◽  
Author(s):  
Qing-You Liu ◽  
Lei Tao ◽  
Hai-Yan Zhu ◽  
Zheng-Dong Lei ◽  
Shu Jiang ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Shale Gas ◽  
Structural Changes ◽  
Triaxial Tests ◽  
Upper Ordovician ◽  
Sem Images ◽  
Bedding Planes ◽  
Before And After ◽  
Supercritical Carbon

Summary Waterless fracturing for shale-gas exploitation using supercritical carbon dioxide (scCO2) is both effective and environmentally friendly, and has become an extensive research topic. Previous researchers have focused on the chemical and physical properties and microstructure of sandstone, carbonate, and shale caprock, rather than on the properties of shale-gas formations. The macroscale mechanical properties and microscale fracture characteristics of Wufeng Shale exposed to scCO2 (at greater than 31.8°C and 7.29 MPa) are still not well-understood. To study the macroscale and microscale changes of shale subjected to scCO2, we obtained Chinese Wufeng Shale crops (Upper Ordovician Formation) from Yibin, Sichuan Basin, China. The shale samples were divided into two groups. The first group was exposed to scCO2, and the second group was exposed to nitrogen (N2). Scanning-electron-microscope (SEM) and X-ray-diffraction (XRD) images were taken to study the original microstructure and mineral content of the shale. To study the macroscale mechanical changes of Wufeng Shale immersed in scCO2 or N2 for 10 hours, triaxial tests with controlled coring angles were conducted. SEM and XRD images were taken after the triaxial tests. In the SEM images, tight bedding planes and undamaged minerals (with sharp edges and smooth surfaces) were found in N2-treated samples both before and after testing, indicating that exposure to N2 did not affect the microstructures. However, the SEM images for the microstructure scCO2-treated samples before and after testing were quite different. The bedding planes were damaged, which left some connected microfractures and corrosion holes, and some mineral types were broken into small particles and left with uneven mineral surfaces. This shows that scCO2 can change rock microstructures and make some minerals (e.g., calcite) fracture more easily. The complex microscale fractures and the decrease in strength for scCO2-treated shale aid the seepage and gathering of gas, enhancing shale-gas recovery. Knowledge of the multiscale physical and chemical changes of shale exposed to scCO2 is not only essential for scCO2 fracturing, but it is also important for scCO2 jets used to break rock and for the geological storage of CO2.

Sign in / Sign up

Export Citation Format

Share Document