scholarly journals Comparative Study on the Effect of Three Disinfection Procedure on the Streptococcus pyogenes Biofilm Formed on Plastic Materials Used in Paedodontics and Orthodontics

2017 ◽  
Vol 54 (1) ◽  
pp. 116-118
Author(s):  
Alexandru Simion Ogodescu ◽  
Alexandru Attila Morvay ◽  
Adriana Balan ◽  
Laura Gavrila ◽  
Ana Petcu ◽  
...  
Keyword(s):  
Streptococcus Pyogenes ◽  
Laser Scanning ◽  
Plastic Material ◽  
Plastic Substrate ◽  
Confocal Laser ◽  
Photodynamic Effect ◽  
Plastic Materials ◽  
Disinfection Procedure ◽  
Oral Microbiology ◽  
Materials Used

Plastic materials are widely used today in Paedodontics and Orthodontics for manufacturing preventive and therapeutic devices. Since these are worn for long times in the oral cavity biofilm forms on the smooth acrylic surfaces of those appliances. The biofilm must be removed not to destroy the oral microbiology. The aim of this study was to research the possibility of removing the microbial biofilm and disinfecting retainers using the photodynamic effect of toluidine blue O, Fotosan System (CMS Dental, Copenhagen, Denmark) in comparison to two products available on the market Corega Denture Cleanser Tablets (GlaxoSmithKline) and the Retainer Brite� Cleaning Tablets (DENTSPLY International Raintree Essix, FL, USA). The plastic material used in this experiment was the cold-cure acrylic Palapress� vario (Heraeus-Kulzer GmbH, Hanau, Germany). Images of the biofilm formed by Streptococcus pyogenes were obtained using a confocal laser scanning m icroscope. The images were analyzed using Comstat 2 software. The results showed that all the three investigated methods had a disinfectant effect. Corega Denture Cleanser Tablets reduced most of the biofilm formed on the plastic substrate.

scholarly journals Cytocompatibility of 3D printed dental materials for temporary restorations on fibroblasts

2020 ◽  
Author(s):  
Jung-Hyun Park ◽  
Hyun Lee ◽  
Jong-Woo Kim ◽  
Ji-Hwan Kim
Keyword(s):  
3D Printing ◽  
Laser Scanning ◽  
Dental Materials ◽  
Three Dimensional ◽  
Confocal Laser Scanning Microscope ◽  
Confocal Laser ◽  
Control Group ◽  
Digital Light Processing ◽  
3D Printed ◽  
Materials Used

Abstract Background Three-dimensional (3D) printing is widely used in the fabrication of dental prostheses; however, the influence of dental materials used for 3D printing on temporary restoration of fibroblasts in tissues is unclear. Thus, the influence of different dental materials on fibroblasts were investigated. Methods Digital light processing (DLP) type 3D printing was used. Specimens in the control group were fabricated by mixing liquid and powder self-curing resin restoration materials. The temporary resin materials used were Model, Castable, Clear-SG, Tray, and Temporary, and the self-curing resin materials used were Lang dental, Alike, Milky blue, TOKVSO CUREFAST, and UniFast III. Fibroblast cells were cultured on each specimen and subsequently post-treated for analysis. Morphology of the adhered cells were observed using a confocal laser scanning microscope (CLSM) and a scanning electron microscope (SEM). Results CLSM and SEM cell imaging revealed that the 3D printed material group presented better cell adhesion with well-distributed filopodia compared to that in the conventional resin material group. Cell proliferation was significantly higher in the 3D printing materials. Conclusion This indicates that using resins fabricated by 3D printing technology rather than the ones fabricated by self-curing technology is recommended for the fabrication of dental temporary restorations.

scholarly journals Cytocompatibility of 3D printed dental materials for temporary restorations on fibroblasts

2020 ◽  
Author(s):  
Jung-Hyun Park ◽  
Hyun Lee ◽  
Jong-Woo Kim ◽  
Ji-Hwan Kim
Keyword(s):  
Cell Proliferation ◽  
Cell Adhesion ◽  
3D Printing ◽  
Laser Scanning ◽  
Dental Materials ◽  
Confocal Laser ◽  
Control Group ◽  
Digital Light Processing ◽  
3D Printed ◽  
Materials Used

Abstract Background Three-dimensional (3D) printing is widely used in the fabrication of dental prostheses; however, the influence of dental materials used for 3D printing on temporary restoration of fibroblasts in tissues is unclear. Thus, the influence of different dental materials on fibroblasts were investigated. Methods Digital light processing (DLP) type 3D printing was used. Specimens in the control group were fabricated by mixing liquid and powder self-curing resin restoration materials. The temporary resin materials used were Model, Castable, Clear-SG, Tray, and Temporary, and the self-curing resin materials used were Lang dental, Alike, Milky blue, TOKVSO CUREFAST, and UniFast III. Fibroblast cells were cultured on each specimen and subsequently post-treated for analysis. Morphology of the adhered cells were observed using a confocal laser scanning microscope (CLSM) and a scanning electron microscope (SEM). Results CLSM and SEM cell imaging revealed that the 3D printed material group presented better cell adhesion with well-distributed filopodia compared to that in the conventional resin material group. Cell proliferation was significantly higher in the 3D printing materials. Conclusion Superior cytocompatibility of the specimens fabricated through 3D printing and polishing process was demonstrated with the proof of better cell adhesion and higher cell proliferation.

scholarly journals The Influence of the Characteristics of Plastic Materials Used in the Performance of the Thoraco-Lumbar Orthoses

2018 ◽  
Vol 55 (1) ◽  
pp. 85-90 ◽  
Author(s):  
Constantin Nanu ◽  
Ion Poeata ◽  
Cezar Popescu ◽  
Lucian Eva ◽  
Bogdan Florin Toma ◽  
...  
Keyword(s):  
Plastic Material ◽  
Burst Fracture ◽  
Rigid Plastic ◽  
Element Analysis ◽  
Plastic Materials ◽  
Plastic Type ◽  
Lumbar Area ◽  
Bone Fragments ◽  
Materials Used ◽  
State Of Tension

The aim of the paper is to identify the of optimal plastic type used in obtaining thoraco-lumbar orthoses - used in the treatments of comminutive fracture, of burst fracture type of the vertebrae in the lumbar area. For this purpose, with the help of Finite Element Analysis (FEA), a theoretical study was carried out on the influence of elastic properties of plastics, used in the achieving of lumbar orthoses, on the state of tension and on the local displacements of the bone fragments from the traumatized area under the condition of the movement from the base of extension and flexion. In the study the force of flexion, the force of extension and the elastic modulus of plastic material varied on three levels. The theoretical results obtained were completed with clinical trials carried out on a total of 26 patients who suffer thoraco-lumbar comminutive fracture, burst fractures type, at vertebra T11 and were immobilized in Boston-type plastic orthoses made of: polypropylene (PP), rigid vinyl polychloride (PVC-D) and polytetrafluoroethylene (PTFE). As a result of observations, it was found that the use of an orthesis made from rigid plastic material, although it appears higher stresses in the traumatized zone, the displacements of bone fragments are smaller, the pains is higher in the fractured zone, the angle of kyphosis (LKA) close to the normal value and a better mobility of the spine (ODI indicator).

scholarly journals Cytocompatibility of 3D printed dental materials for temporary restorations on fibroblasts

2020 ◽  
Author(s):  
Jung-Hyun Park ◽  
Hyun Lee ◽  
Jong-Woo Kim ◽  
Ji-Hwan Kim
Keyword(s):  
Cell Proliferation ◽  
Cell Adhesion ◽  
3D Printing ◽  
Laser Scanning ◽  
Dental Materials ◽  
Confocal Laser ◽  
Control Group ◽  
Digital Light Processing ◽  
3D Printed ◽  
Materials Used

Abstract Background Three-dimensional (3D) printing is widely used in the fabrication of dental prostheses; however, the influence of dental materials used for 3D printing on temporary restoration of fibroblasts in tissues is unclear. Thus, the influence of different dental materials on fibroblasts were investigated. Methods Digital light processing (DLP) type 3D printing was used. Specimens in the control group were fabricated by mixing liquid and powder self-curing resin restoration materials. The temporary resin materials used were Model, Castable, Clear-SG, Tray, and Temporary, and the self-curing resin materials used were Lang dental, Alike, Milky blue, TOKVSO CUREFAST, and UniFast III. Fibroblast cells were cultured on each specimen and subsequently post-treated for analysis. Morphology of the adhered cells were observed using a confocal laser scanning microscope (CLSM) and a scanning electron microscope (SEM). Results CLSM and SEM cell imaging revealed that the 3D printed material group presented better cell adhesion with well-distributed filopodia compared to that in the conventional resin material group. Cell proliferation was significantly higher in the 3D printing materials. Conclusion Superior cytocompatibility of the specimens fabricated through 3D printing and polishing process was demonstrated with the proof of better cell adhesion and higher cell proliferation.

3-D imaging of cells using a confocal laser scanning microscope and digital image processing

1988 ◽  
Vol 46 ◽  
pp. 96-97
Author(s):  
Thomas M. Jovin ◽  
Michel Robert-Nicoud ◽  
Donna J. Arndt-Jovin ◽  
Thorsten Schormann
Keyword(s):  
Laser Scanning ◽  
Confocal Laser Scanning Microscope ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Scanning Microscope ◽  
Laser Scanning Microscope ◽  
Biochemical Processes ◽  
Adjacent Structures

Light microscopic techniques for visualizing biomolecules and biochemical processes in situ have become indispensable in studies concerning the structural organization of supramolecular assemblies in cells and of processes during the cell cycle, transformation, differentiation, and development. Confocal laser scanning microscopy offers a number of advantages for the in situ localization and quantitation of fluorescence labeled targets and probes: (i) rejection of interfering signals emanating from out-of-focus and adjacent structures, allowing the “optical sectioning” of the specimen and 3-D reconstruction without time consuming deconvolution; (ii) increased spatial resolution; (iii) electronic control of contrast and magnification; (iv) simultanous imaging of the specimen by optical phenomena based on incident, scattered, emitted, and transmitted light; and (v) simultanous use of different fluorescent probes and types of detectors.We currently use a confocal laser scanning microscope CLSM (Zeiss, Oberkochen) equipped with 3-laser excitation (u.v - visible) and confocal optics in the fluorescence mode, as well as a computer-controlled X-Y-Z scanning stage with 0.1 μ resolution.

3-Dimensional immunolabeling and in situ hybridization detection using fluorescence photooxidation and intermediate-voltage Electron Microscopy

1994 ◽  
Vol 52 ◽  
pp. 164-165
Author(s):  
Thomas J. Deerinck ◽  
Maryann E. Martone ◽  
Varda Lev-Ram ◽  
David P. L. Green ◽  
Roger Y. Tsien ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Reactive Oxygen ◽  
Confocal Laser Scanning Microscope ◽  
Fluorescent Dye ◽  
Confocal Laser ◽  
3 Dimensional ◽  
Voltage Electron ◽  
Dimensional Distribution ◽  
Electron Microscopes ◽  
New Generation

The confocal laser scanning microscope has become a powerful tool in the study of the 3-dimensional distribution of proteins and specific nucleic acid sequences in cells and tissues. This is also proving to be true for a new generation of high contrast intermediate voltage electron microscopes (IVEM). Until recently, the number of labeling techniques that could be employed to allow examination of the same sample with both confocal and IVEM was rather limited. One method that can be used to take full advantage of these two technologies is fluorescence photooxidation. Specimens are labeled by a fluorescent dye and viewed with confocal microscopy followed by fluorescence photooxidation of diaminobenzidine (DAB). In this technique, a fluorescent dye is used to photooxidize DAB into an osmiophilic reaction product that can be subsequently visualized with the electron microscope. The precise reaction mechanism by which the photooxidation occurs is not known but evidence suggests that the radiationless transfer of energy from the excited-state dye molecule undergoing the phenomenon of intersystem crossing leads to the formation of reactive oxygen species such as singlet oxygen. It is this reactive oxygen that is likely crucial in the photooxidation of DAB.

Vacuoles Induced in Mammalian Cells by Helicobacter Cytotoxin are Acidic Organelles

1990 ◽  
Vol 48 (3) ◽  
pp. 168-169
Author(s):  
M. H. Chestnut ◽  
C. E. Catrenich
Keyword(s):  
Acridine Orange ◽  
Mammalian Cells ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Ulcer Disease ◽  
Gastroduodenal Disease ◽  
H Pylori

Helicobacter pylori is a non-invasive, Gram-negative spiral bacterium first identified in 1983, and subsequently implicated in the pathogenesis of gastroduodenal disease including gastritis and peptic ulcer disease. Cytotoxic activity, manifested by intracytoplasmic vacuolation of mammalian cells in vitro, was identified in 55% of H. pylori strains examined. The vacuoles increase in number and size during extended incubation, resulting in vacuolar and cellular degeneration after 24 h to 48 h. Vacuolation of gastric epithelial cells is also observed in vivo during infection by H. pylori. A high molecular weight, heat labile protein is believed to be responsible for vacuolation and to significantly contribute to the development of gastroduodenal disease in humans. The mechanism by which the cytotoxin exerts its effect is unknown, as is the intracellular origin of the vacuolar membrane and contents. Acridine orange is a membrane-permeant weak base that initially accumulates in low-pH compartments. We have used acridine orange accumulation in conjunction with confocal laser scanning microscopy of toxin-treated cells to begin probing the nature and origin of these vacuoles.

A confocal laser scanning microscope for fluorescence and reflection at video rates

1989 ◽  
Vol 47 ◽  
pp. 134-135
Author(s):  
P.M. Houpt ◽  
A. Draaijer
Keyword(s):  
Light Source ◽  
Laser Scanning ◽  
Focal Point ◽  
Confocal Laser Scanning Microscope ◽  
Confocal Laser ◽  
Point Light Source ◽  
Volume Of Interest ◽  
Ion Laser ◽  
Point Light ◽  
Point Detector

In confocal microscopy, the object is scanned by the coinciding focal points (confocal) of a point light source and a point detector both focused on a certain plane in the object. Only light coming from the focal point is detected and, even more important, out-of-focus light is rejected.This makes it possible to slice up optically the ‘volume of interest’ in the object by moving it axially while scanning the focused point light source (X-Y) laterally. The successive confocal sections can be stored in a computer and used to reconstruct the object in a 3D image display.The instrument described is able to scan the object laterally with an Ar ion laser (488 nm) at video rates. The image of one confocal section of an object can be displayed within 40 milliseconds (1000 х 1000 pixels). The time to record the total information within the ‘volume of interest’ normally depends on the number of slices needed to cover it, but rarely exceeds a few seconds.

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