scholarly journals In Vitro Asymbiotic Germination for Micropropagation of the Recalcitrant Terrestrial OrchidChloraea crispa(Orchidaceae)

2017 ◽  
Vol 5 (8) ◽  
pp. 1600142 ◽  
Author(s):  
Karla Quiroz ◽  
Jessica Saavedra ◽  
Hermine Vogel ◽  
Gabriela Verdugo ◽  
Peter D. S. Caligari ◽  
...  
Keyword(s):  
Asymbiotic Germination

scholarly journals Asymbiotic germination and morphological studies of seeds of Atlantic Rainforest micro-orchids (Pleurothallidinae)

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0243297
Author(s):  
Franciele Marx Koene ◽  
Érika Amano ◽  
Eric de Camargo Smidt ◽  
Luciana Lopes Fortes Ribas
Keyword(s):  
Developmental Stages ◽  
Morphometric Study ◽  
Morphometric Characters ◽  
Orchid Species ◽  
Morphological Studies ◽  
Germination Process ◽  
Length Width ◽  
Asymbiotic Germination ◽  
Protocorm Formation

The morphological and morphometric characters of seeds belonging to 11 species of the subtribe Pleurothallidinae using light and scanning electron microscopy were studied to understand the in vitro germination process. Qualitative data (color, shape, ornamentation) and quantitative ones were also evaluated in seeds and embryos (length, width, volume and air space percentage between the integument and the embryo). The viability of the seeds was evaluated by in vitro germination in woody plant medium (WPM), and by analysis of the developmental stages of protocorms until seedling formation (two to 24 weeks). Morphometric data showed variations within the genus Acianthera and between species of different genera. The best germination and protocorm formation responses occurred with Acianthera prolifera (92%) and Acianthera ochreata (86%), with the formation of seedlings after 12 and 16 weeks of sowing, respectively. The seeds and embryos of A. prolifera and A. ochreata were larger (length, width, and volume) with a structural polarity that may have facilitated their germination comparing to others studied species. Other characteristics of A. prolifera seeds that may have contributed to these results include the presence of a thin testa without ornamentation and a suspensor. The protocorms of Anathalis obovata, Dryadella liliputiana, and Octomeria gracillis developed slowly in the WPM, not reaching the seedling stage in 24 weeks of cultivation. This morphological and morphometric study contributes to the understanding of asymbiotic germination of some micro-orchid species.

scholarly journals Asymbiotic in vitro germination and seed quality assessment of Australian terrestrial orchids

2012 ◽  
Vol 60 (7) ◽  
pp. 592 ◽  
Author(s):  
Nicole Dowling ◽  
Manfred Jusaitis
Keyword(s):  
Seed Quality ◽  
Culture Media ◽  
Vital Staining ◽  
Terrestrial Orchid ◽  
Triphenyltetrazolium Chloride ◽  
Asymbiotic Seed Germination ◽  
Orchid Species ◽  
Terrestrial Orchids ◽  
Asymbiotic Germination

Determining the seed quality and germination requirements for threatened orchid species in storage is vital for future conservation efforts. Seeds of many Australian terrestrial orchid species are held in conservation collections around the country, but few have been germinated in vitro, fuelling concerns over their long-term viability. This study tested three methods of assessing orchid seed quality; asymbiotic germination was compared with vital staining using triphenyltetrazolium chloride or fluorescein diacetate. Six culture media were examined for efficacy in promoting asymbiotic seed germination of four Australian terrestrial orchid species (Pterostylis nutans, Microtis arenaria, Thelymitra pauciflora and Prasophyllum pruinosum). Germination occurred on all media but germination rates were consistently highest on BM1 and development was most advanced on BM1, P723 and Malmgren media. Subsequent trials tested the efficacy of BM1 for asymbiotic germination of additional genera (Caladenia, Calochilus and Diuris), several congeneric species, and two species collected from several different provenances within each of their ranges. The results indicate that asymbiotic germination on BM1 medium is an effective technique for testing the performance of Australian terrestrial orchid seeds. The efficacy of vital stains to determine seed viability, however, remains uncertain, as significant disagreement between degree of staining and germinability was observed for some species.

scholarly journals Effects of wavelength of LED-light on in vitro asymbiotic germination and seedling growth of Bletilla ochracea Schltr. (Orchidaceae)

2011 ◽  
Vol 28 (4) ◽  
pp. 397-400 ◽  
Author(s):  
Toshinari Godo ◽  
Kazuhiro Fujiwara ◽  
Kaiyun Guan ◽  
Kazumitsu Miyoshi
Keyword(s):  
Seedling Growth ◽  
Led Light ◽  
Asymbiotic Germination
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