scholarly journals Development and Characterization of 25 Microsatellite Primers for Ilex chinensis (Aquifoliaceae)

2015 ◽  
Vol 3 (10) ◽  
pp. 1500057 ◽  
Author(s):  
Wen-Wen Chen ◽  
Zhen-Zhu Xiao ◽  
Xin Tong ◽  
Yu-Pei Liu ◽  
Yuan-Yuan Li
Keyword(s):  
Microsatellite Primers

scholarly journals Agronomical and molecular characterization of banana germplasm

2010 ◽  
Vol 45 (2) ◽  
pp. 146-154 ◽  
Author(s):  
Lorenna Alves Mattos ◽  
Edson Perito Amorim ◽  
Vanusia Batista de Oliveira Amorim ◽  
Kelly de Oliveira Cohen ◽  
Carlos Alberto da Silva Ledo ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Physicochemical Characteristics ◽  
High Variability ◽  
Microsatellite Primers ◽  
Germplasm Bank ◽  
B Genome ◽  
Agronomical Traits ◽  
Simple Sequence ◽  
Agronomical Characters

The objective of the present work was to characterize banana accessions from the Germplasm Bank at Embrapa Mandioca e Fruticultura Tropical (Brazil), using agronomical, physical and physicochemical characteristics of fruit and simple sequence repeats (SSR) markers. Twenty-six accessions were analyzed, in which high genetic variability was found, especially for the agronomical characters number of fruit and weight of bunch. Accessions with high contents of carotenoids (diploid 'Jaran'), polyphenols (triploid 'Caipira' and tetraploid 'Teparod') and vitamin C (diploid 'Tuugia' and an unknown triploid AAA) in the fruit were identified. Thirteen microsatellite primers revealed an average of 7.23 alleles, which showed high variability. A dendrogram was prepared using the Gower algorithm for the distance matrices obtained from the agronomical, physical and physicolchemical analysis of fruit and SSR markers. Adopting the average genetic divergence as the cut-off point, three clusters were found: G1, formed by the diploids 'Jaran', 028003-01 and M-48; G2, by the diploids 'Malbut' and 'Ido 110'; and G3, by 21 tri-and tetraploid accessions, including one diploid, 'Tuugia'. The triploids with the B genome 'Thap Maeo', 'Walha', 'Pacha Nadan' and 'Champa Madras' were grouped in G2. Results from this work can be used for breeding hybrids with good agronomical traits and fruit quality.

scholarly journals Identification and characterization of 17 microsatellite primers for the tick, Ixodes ricinus, using enriched genomic libraries

2006 ◽  
Vol 6 (4) ◽  
pp. 1165-1167 ◽  
Author(s):  
KNUT H. RØED ◽  
GUNNAR HASLE ◽  
V. MIDTHJELL ◽  
GRETHE SKRETTING ◽  
HANS P. LEINAAS
Keyword(s):  
Ixodes Ricinus ◽  
Microsatellite Primers ◽  
Genomic Libraries ◽  
Identification And Characterization

Isolation and characterization of nuclear microsatellite primers for the Barbary thuja, Tetraclinis articulata (Vahl) Mast. (Cupressaceae)

2013 ◽  
Vol 6 (1) ◽  
pp. 233-235
Author(s):  
María Teresa Lorenzo ◽  
Ramón Casimiro-Soriguer ◽  
Francisco Balao ◽  
Juan Luis García-Castaño ◽  
Jose M. Sánchez-Robles ◽  
...  
Keyword(s):  
Microsatellite Primers ◽  
Isolation And Characterization ◽  
Tetraclinis Articulata ◽  
Nuclear Microsatellite

scholarly journals Development and Characterization of Microsatellite Primers inGeranium carolinianum(Geraniaceae) with 454 Sequencing

2013 ◽  
Vol 1 (8) ◽  
pp. 1300006 ◽  
Author(s):  
Rebecca Y. Shirk ◽  
Travis C. Glenn ◽  
Shu-Mei Chang ◽  
J. L. Hamrick
Keyword(s):  
454 Sequencing ◽  
Microsatellite Primers

scholarly journals Characterization of Nuclear Microsatellite Markers for the Narrow Endemic Syringa josikaea Jacq. fil. ex Rchb.

2013 ◽  
Vol 41 (1) ◽  
pp. 301 ◽  
Author(s):  
Bertalan LENDVAY ◽  
Andrzej PEDRYC ◽  
Mária Höhn
Keyword(s):  
Microsatellite Markers ◽  
Ornamental Plants ◽  
Enriched Library ◽  
Microsatellite Primers ◽  
Genetic Lineages ◽  
Nuclear Microsatellite ◽  
Rare Endemic ◽  
Two Populations ◽  
Pcr Conditions

The species of the genus Syringa L. are among the most popular ornamental plants worldwide. One particular species, Syringa josikaea, a rare endemic of the Carpathian Mountains, is of great conservation interest. Although microsatellite markers may be useful for studying the genetic variability of varieties and populations, no microsatellites have previously been characterized for any species of the Syringa genus. Our aim was therefore to test the applicability of microsatellite primers developed for neighboring genera (Olea and Ligustrum) and to complement these with markers isolated and characterized for S. josikaea. Twelve primer pairs of Olea and Ligustrum were tested by optimizing PCR conditions and checking the variability in 40 samples of two populations of S. josikaea. Two of them proved to be easy to PCR amplify and variable at the same time. To develop new primers we constructed a microsatellite enriched library and sequenced 48 clones. 18 sequences contained microsatellite motifs, and three of the designed primer pairs presented high allele variability. The five primer pairs characterized for S. josikaea proved to be highly informative and sufficient to distinguish between individuals. These microsatellite primers are valuable tools to study genetic variation of native populations, genetic lineages of hybrids and cultivars of S. josikaea.

scholarly journals Development and Characterization of Microsatellite Primers in the Federally EndangeredAstragalus bibullatus(Fabaceae)

2016 ◽  
Vol 4 (4) ◽  
pp. 1500126 ◽  
Author(s):  
Ashley B. Morris ◽  
Cassandra Scalf ◽  
Austin Burleyson ◽  
La Tonya Johnson ◽  
Kevin Trostel
Keyword(s):  
Microsatellite Primers

Development and characterization of polymorphic microsatellite markers for Spathoglottis plicata (Orchidaceae)

2018 ◽  
Vol 16 (6) ◽  
pp. 572-575
Author(s):  
Chiuan-Yu Li ◽  
Chi-Chun Huang ◽  
Chaur-Tzuhn Chen ◽  
Kuo-Hsiang Hung
Keyword(s):  
Microsatellite Markers ◽  
Microsatellite Primers ◽  
Wild Populations ◽  
Terrestrial Orchid ◽  
Chain Reaction ◽  
Expected Heterozygosity ◽  
Genetic Patterns ◽  
Polymerase Chain ◽  
Polymorphic Microsatellite

AbstractWe developed novel and polymorphic microsatellite primers for Spathoglottis plicata, a tropical and subtropical terrestrial orchid, to investigate the genetic patterns and population structure among wild populations, and also to identify the varieties and hybrids of S. plicata in horticultural industry. The 12 novel microsatellites from S. plicata were developed by using polymerase chain reaction (PCR)-based isolation of microsatellite arrays. These markers that were successfully PCR amplified exhibited polymorphisms in S. plicata. The number of alleles, observed heterozygosity, expected heterozygosity and polymorphism information content values across loci ranged from 2.000 to 8.000, 0.000 to 0.756, 0.208 to 0.813 and 0.405 to 0.805 in total populations, respectively. The newly developed microsatellite markers exhibited variation in S. plicata. These markers can be used as a tool to further investigate the genetic diversity, conservation genetics and variety/hybrid identification of S. plicata.

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