scholarly journals Development of Genomic Microsatellites inGleditsia triacanthos(Fabaceae) Using Illumina Sequencing

2013 ◽  
Vol 1 (12) ◽  
pp. 1300050 ◽  
Author(s):  
Sandra A. Owusu ◽  
Margaret Staton ◽  
Tara N. Jennings ◽  
Scott Schlarbaum ◽  
Mark V. Coggeshall ◽  
...  
Keyword(s):  
Illumina Sequencing ◽  
Genomic Microsatellites

scholarly journals Development of genic and genomic microsatellites in Gleditsia triacanthos L. (Fabaceae) using Illumina sequencing

2017 ◽  
Vol 0 (0) ◽  
Author(s):  
Yawen Wu ◽  
Ruhua Zhang ◽  
Margaret Staton ◽  
Scott E. Schlarbaum ◽  
Mark V. Coggeshall ◽  
...  
Keyword(s):  
Illumina Sequencing ◽  
Gleditsia Triacanthos ◽  
Genomic Microsatellites

scholarly journals Diversity level of genomic microsatellites in redbay (Perseaborbonia L.) generated by Illumina sequencing

2015 ◽  
Vol 4 (1) ◽  
pp. 2 ◽  
Author(s):  
Chien-Chih Chen ◽  
Yi Xu ◽  
Tao Xu ◽  
Margaret Staton ◽  
Garrick Stott ◽  
...  
Keyword(s):  
Illumina Sequencing ◽  
Genomic Microsatellites

scholarly journals Microbiome-based environmental monitoring of a dairy processing facility highlights the challenges associated with low microbial-load samples

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Aoife J. McHugh ◽  
Min Yap ◽  
Fiona Crispie ◽  
Conor Feehily ◽  
Colin Hill ◽  
...  
Keyword(s):  
Environmental Monitoring ◽  
Illumina Sequencing ◽  
Food Chain ◽  
Control Measures ◽  
Initial Assessment ◽  
Limiting Factor ◽  
Accurate Identification ◽  
Processing Facility ◽  
High Concentrations ◽  
Sequencing Platforms

AbstractEfficient and accurate identification of microorganisms throughout the food chain can potentially allow the identification of sources of contamination and the timely implementation of control measures. High throughput DNA sequencing represents a potential means through which microbial monitoring can be enhanced. While Illumina sequencing platforms are most typically used, newer portable platforms, such as the Oxford Nanopore Technologies (ONT) MinION, offer the potential for rapid analysis of food chain microbiomes. Initial assessment of the ability of rapid MinION-based sequencing to identify microbes within a simple mock metagenomic mixture is performed. Subsequently, we compare the performance of both ONT and Illumina sequencing for environmental monitoring of an active food processing facility. Overall, ONT MinION sequencing provides accurate classification to species level, comparable to Illumina-derived outputs. However, while the MinION-based approach provides a means of easy library preparations and portability, the high concentrations of DNA needed is a limiting factor.

scholarly journals Raw pacific biosciences and illumina sequencing reads and assembled genome data for the cattle ticks Rhipicephalus microplus and Rhipicephalus annulatus

Data in Brief ◽  
2021 ◽  
Vol 35 ◽  
pp. 106852
Author(s):  
Felix D. Guerrero ◽  
Noushin Ghaffari ◽  
Kylie G. Bendele ◽  
Richard P. Metz ◽  
C. Michael Dickens ◽  
...  
Keyword(s):  
Illumina Sequencing ◽  
Rhipicephalus Microplus ◽  
Pacific Biosciences ◽  
Genome Data ◽  
Cattle Ticks ◽  
Rhipicephalus Annulatus

Structural families of genomic microsatellites

Gene ◽  
2008 ◽  
Vol 408 (1-2) ◽  
pp. 124-132 ◽  
Author(s):  
Juan A. Subirana ◽  
Xavier Messeguer
Keyword(s):  
Genomic Microsatellites

Diversity of Prokaryotic Community at a Shallow Marine Hydrothermal Site Elucidated by Illumina Sequencing Technology

2014 ◽  
Vol 69 (4) ◽  
pp. 457-466 ◽  
Author(s):  
Valeria Lentini ◽  
Concetta Gugliandolo ◽  
Boyke Bunk ◽  
Jörg Overmann ◽  
Teresa L. Maugeri
Keyword(s):  
Illumina Sequencing ◽  
Prokaryotic Community ◽  
Sequencing Technology ◽  
Shallow Marine

Illumina Sequencing Reveals Conserved and Novel MicroRNAs of Dendrobium nobile Protocorm Involved in Synthesizing Dendrobine, a Potential Nanodrug

2021 ◽  
Vol 17 (3) ◽  
pp. 416-425
Author(s):  
Xu Qian ◽  
Jieying Zhu ◽  
Qingjun Yuan ◽  
Qi Jia ◽  
Hui Jin ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Illumina Sequencing ◽  
Plant Hormone ◽  
Signal Transduction Pathway ◽  
Perennial Herb ◽  
Transduction Pathway ◽  
Biological Regulation ◽  
Dendrobium Nobile ◽  
Short Period ◽  
Post Transcriptional Regulation

Emergency of nanoparticulate drug delivery systems has improved the target, bioavailability, and curative effect of traditional Chinese medicine (TCM). However, the application of nano-preparation has been limited owing to the low content of active ingredients in part TCM. MicroRNAs (miRNAs) regulate plant growth, development, and response to environmental stresses at post-transcriptional regulation level by cleavage or translational inhibition. The molecular functions of miRNAs playing a role in synthesizing active comportments at medicinal plants have been widely researched. Dendrobium nobile is a perennial herb in the orchidaceae family. D. nobile protocorm can produce plant-specific metabolites at a short period. Therefore, it is a good substitute for producing metabolites. To understand the functions of miRNAs in D. nobile protocorm, Illumina sequencing of D. nobile protocorm (Dnp), D. officinale protocorm (Dcp), and D. nobile leaf (Dnl) were carried out. A total of 439, 412, and 432 miRNAs were identified from Dnp, Dcp, and Dnl, respectively. Some specific miRNAs were identified among them. Through combing GO and KEGG function annotation, miRNAs mainly involved metabolic pathways, plant hormone signal transduction, biological regulation, and protein binding. Acetyl-CoA acetyltransferase (AACT), mevalonate kinase (MK), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR), and 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (HDS), synthesizing basic precursor isoprene pyrophosphate (IPP) in terpenoid backbone biosynthesis pathway, were predicted as potential targets of 6 different miRNAs. Twenty-six miRNAs participated in auxin, cytokinin, abscisic acid, jasmonic acid, and salicylic acid signal transduction pathway. This report provided valuable candidate genes in Dnp involved in terpenoid biosynthesis and plant hormone signal transduction pathway. At the same time, it can help accelerate the use of dendrobine into nano preparation.

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