scholarly journals Production of biodegradable film from soy protein and essential oil of lemon peel and use it as cheese preservative

2017 ◽  
Vol 30 (2) ◽  
pp. 27-35 ◽  
Author(s):  
Shayma T. G. Al-Sahlany
Keyword(s):  
Essential Oil ◽  
Soy Protein ◽  
Inhibition Activity ◽  
Bacterial Strains ◽  
Biodegradable Film ◽  
Protein Film ◽  
Main Compound ◽  
E Coli ◽  
Lemon Peel ◽  
White Cheese

Soy protein with essential oil of lemon peel was used to produce a biodegradable film, which showed inhibition activity against some species of selected bacterial strains. The films were white cheese coating and studied for its biodegradation in the soil. The percentage of soy protein extract was 15% (W: W) while the percentage of lemon peel essential oil was 3% (V: W). The GC-MS result of lemon peel essential oil consisted of 38 compounds, of which the main compound is D-Limonene in 63.43%. The films produced showed inhibition activity against both Gram-positive and Gramnegative bacteria. Inhibition activity was highest against Staphylococcus aureus (22 mm zone), while E. coli O157: H7 had the lowest inhibition (18mm) when 0.1mL of essential oil with 10mL film mixed. The coating process of white cheese reduced the total microbial population (CFU/g) during 30 days of storage time. The TCB, TC, ST and MY of cheese coating were 4.28, 1.53, 1.62 and 1.33 log. CFU/g respectively after 30 days compared to the cheese non-coating samples, with 6.53, 2.51, 3.17 and 1.81 log. CFU/g, respectively. The biodegradation in soil was 97 and 72% for soy protein film and soy protein film with lemon peel essential oil after 35 days.

scholarly journals Antimicrobial activities and mechanism of action of Cymbopogon khasianus (Munro ex Hackel) Bor essential oil

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Gurpreet Singh ◽  
Meenu Katoch
Keyword(s):  
Essential Oil ◽  
Control Measures ◽  
Food Storage ◽  
Major Constituent ◽  
Haemolytic Activity ◽  
Human Pathogens ◽  
Health Food ◽  
Bacterial Strains ◽  
Safe Alternative ◽  
E Coli

Abstract Background Due to concerns regarding the safety of the chemical control measures, the trend is shifting globally towards the use of natural compounds as antimicrobial agent especially, plant essential oils. Results This study presented the antibacterial potential of Cymbopogon khasianus essential oil (CKEO) against human pathogens: Pseudomonas aeruginosa, Salmonella typhimurium, Escherichia coli, Klebsiella pneumoniae, Bacillus subtilis, Staphylococcus aureus and Candida albicans with MIC ranging from 20 to 100 μg/mL. CKEO, in comparison to its major constituent, geraniol, showed better MICs against tested pathogens. In combination studies, the effective concentrations of CKEO and streptomycin were reduced from 20 to 5 μg/mL and 11 to 0.7 ng/mL against E. coli. This suggests their synergistic action. However, CKEO showed partial synergy with ciprofloxacin. To understand the efficacy of CKEO, time-kill kinetics was performed. CKEO took the half time to show the bactericidal effect in comparison to streptomycin at their 2x MICs (double the MIC), while their combination took only 30 min for this. Fluorescence and surface electron microscopic and protein estimation studies suggested the multi-target action of CKEO-streptomycin combination against E. coli. Further, CKEO alone/in combination exhibited less than 10% haemolytic activity at its MIC. Conclusion These results indicate that CKEO is a potentially safe alternative for the treatment of various pathogenic bacterial strains. It could be used for a variety of applications including human health, food storage, aquaculture, etc.

scholarly journals Modulating the Precursor and Terpene Synthase Supply for the Whole-Cell Biocatalytic Production of the Sesquiterpene (+)-Zizaene in a Pathway Engineered E. coli

Genes ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 478 ◽  
Author(s):  
Francisco Aguilar ◽  
Thomas Scheper ◽  
Sascha Beutel
Keyword(s):  
Essential Oil ◽  
Metabolic Flux ◽  
Mevalonate Pathway ◽  
Terpene Synthase ◽  
Methylerythritol Phosphate Pathway ◽  
Main Compound ◽  
E Coli ◽  
Methylerythritol Phosphate ◽  
Cosmetic Industry ◽  
Multiple Copies

The vetiver essential oil from Chrysopogon zizanioides contains fragrant sesquiterpenes used widely in the formulation of nearly 20% of men’s cosmetics. The growing demand and issues in the supply have raised interest in the microbial production of the sesquiterpene khusimol, the main compound of the vetiver essential oil due to its woody smell. In this study, we engineered the biosynthetic pathway for the production of (+)-zizaene, the immediate precursor of khusimol. A systematic approach of metabolic engineering in Escherichia coli was applied to modulate the critical bottlenecks of the metabolic flux towards (+)-zizaene. Initially, production of (+)-zizaene was possible with the endogenous methylerythritol phosphate pathway and the codon-optimized zizaene synthase (ZS). Raising the precursor E,E-farnesyl diphosphate supply through the mevalonate pathway improved the (+)-zizaene titers 2.7-fold, although a limitation of the ZS supply was observed. To increase the ZS supply, distinct promoters were tested for the expression of the ZS gene, which augmented 7.2-fold in the (+)-zizaene titers. Final metabolic enhancement for the ZS supply by using a multi-plasmid strain harboring multiple copies of the ZS gene improved the (+)-zizaene titers 1.3-fold. The optimization of the fermentation conditions increased the (+)-zizaene titers 2.2-fold, achieving the highest (+)-zizaene titer of 25.09 mg L−1. This study provides an alternative strategy to enhance the terpene synthase supply for the engineering of isoprenoids. Moreover, it demonstrates the development of a novel microbial platform for the sustainable production of fragrant molecules for the cosmetic industry.

scholarly journals Escherichia coli and Staphylococcus aureus responsible for bovine mastitis sensitivity to the essential oil of Algerian Thymus fontanesii Boiss. et Reut.

2020 ◽  
Vol 7 (1) ◽  
pp. 26-32
Author(s):  
Bendella Amina nor elhouda ◽  
Ghazi Kheira ◽  
Meliani Samia
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Bovine Mastitis ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Zones Of Inhibition

AbstractThe aim of this study is to test two different methods for evaluating the in vitro antibacterial effect of Thymus fontanesii Boiss. et Reut. essential oil against standard and clinical bacterial strains responsible for bovine mastitis: the disc diffusion method or the aromatogram which allows the demonstration of the antibacterial power of essential oils on the bacterial strains tested, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and two strains isolated from bovine mastitis milk S. aureus and E. coli. The inhibition activity of the essential oil of T. fontanesii on bacterial strains by the two methods shows that the antimicrobial power of this oil is very important and is characterized by bactericidal and bacteriostatic action against gram negative and gram positive bacteria. The antimicrobial evaluation by the aromatogram showed good antibacterial activity against all the strains tested, the zones of inhibition of the bacteria were between 23,33±1,527mm and 37,5±3,535 mm. The search for minimum inhibitory concentrations MIC and bactericides CMB made it possible to quantitatively assess the antimicrobial power of this essential oil. In this work, the MIC was 0,625 µl/ml for all strains tested, and the lowest CMB was that of T. fontanesii against E. coli ATCC 25922 was 0,625 µl/ml.

scholarly journals β-Lactamase inhibition profile of new amidine-substituted diazabicyclooctanes

2021 ◽  
Vol 17 ◽  
pp. 711-718
Author(s):  
Zafar Iqbal ◽  
Lijuan Zhai ◽  
Yuanyu Gao ◽  
Dong Tang ◽  
Xueqin Ma ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Second Generation ◽  
Bacterial Species ◽  
The Other ◽  
Inhibition Activity ◽  
Bacterial Strains ◽  
E Coli ◽  
Mic Value

The diazabicyclooctane (DBO) scaffold is the backbone of non-β-lactam-based second generation β-lactamase inhibitors. As part of our efforts, we have synthesized a series of DBO derivatives A1–23 containing amidine substituents at the C2 position of the bicyclic ring. These compounds, alone and in combination with meropenem, were tested against ten bacterial strains for their antibacterial activity in vitro. All compounds did not show antibacterial activity when tested alone (MIC >64 mg/L), however, they exhibited a moderate inhibition activity in the presence of meropenem by lowering its MIC values. The compound A12 proved most potent among the other counterparts against all bacterial species with MIC from <0.125 mg/L to 2 mg/L, and is comparable to avibactam against both E. coli strains with a MIC value of <0.125 mg/L.

scholarly journals Essential Oil Yield Pattern and Antibacterial and Insecticidal Activities ofTrachyspermum ammiandMyristica fragrans

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Rajgovind Soni ◽  
Gaurav Sharma ◽  
Nakuleshwar Dut Jasuja
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
E Coli ◽  
Chromatography Analysis ◽  
Alpha Pinene ◽  
Gas Chromatography Analysis ◽  
Insecticidal Activities

Two Indian spices,Trachyspermum ammiandMyristica fragrans,were studied for their essential oil (EO) yielding pattern, insecticidal activity, antibacterial activity, and composition. The essential oils (EOs) ofT. ammi(1.94±30 mL/100 gm) andM. fragrans(5.93±90 mL/100 gm) were extracted using hydrodistillation method. In Gas Chromatography analysis, the beta-pinene, alpha-pinene, alpha-p-menth-1-en-4-ol, Limonene, and elemicin were found as major constituents ofT. ammiessential oil whereasM. fragransessential oil mostly contains Gamma-Terpinolene, p-Cymene, Thymol, and beta-pinene. The insecticidal activities of EO were demonstrated using LC50values againstPlodia interpunctellaand EO ofT. ammiwas found comparatively more effective than EO ofM. fragrans.Further, individual EO and combination of essential oil were examined for antibacterial activity against three Gram (−) bacterial strains (E. coli-MTCC 443,P. vulgaris-MTCC 1771, andK. pneumoniae-MTCC number 7028) and three Gram (+) bacterial strains (S. aureus-MTCC 3381,B. subtilis-MTCC 10619, andB. megaterium-MTCC 2412) by well agar diffusion method. The essential oil in combination (CEO) exhibited higher antibacterial activity as compared with individual essential oils.

scholarly journals The effect of cold aqueous extract of lemon peel against types of bacteria isolated from the cooling devices Filters

2017 ◽  
Vol 28 (1) ◽  
pp. 70
Author(s):  
Ashraf S. Hassan
Keyword(s):  
Aqueous Extract ◽  
Bacterial Species ◽  
Water Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Citrus Peel ◽  
E Coli ◽  
Lemon Peel ◽  
Bacillus Spp

The antibacterial effect of citrus peel lemon against the bacterial strains obtained from the filters of air conditioners have been selected based on the presence most in these filters such as Streptococcus, Bacillus spp, Pseudomonas, E coli. agar well diffusion method used to evaluate antibacterial activity of citrus peels water extract. through the results became clear to us that the cold aqueous extract of lemon peel showed a significant effect on the growth of bacterial species through the diameters of inhibition zone that appeared in all concentrations of the extract (125, 250, 500, 1000) mg/ml. Gram-positive bacteria Streptococcus spp were the most affected Where the diameters of inhibition zone (18, 15, 12, 0, 0) mm respectively, while The Gram-negative bacteria E coli least affected. The results obtained in this study indicate that citrus lemon peel can be used in the treatment of diseases caused by organisms for the purposes of the pharmaceutical.

scholarly journals Chemical Composition and Antibacterial Activity of Berries Essential Oil of Algerian Juniperus thurifera (Var. aurasiaca)

2018 ◽  
Vol 24 (3) ◽  
pp. 240-245
Author(s):  
Lamia Boudjedjou ◽  
Messaoud Ramdani ◽  
Azzeddine Zeraib ◽  
Tarek Benmeddour ◽  
Azzedine Fercha
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Synergistic Effects ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Juniperus Thurifera ◽  
E Coli ◽  
Conventional Antibiotics ◽  
Promising Source

Background: Over the past decade, most antibiotic research programs have focused on finding new compounds with antimicrobial activity. This study aims to investigate the chemical composition and antibacterial activity of the essential oil (EO) extracted from ripe berries of Algerian Juniperus thurifera var. aurasiaca. Methods: The chemical composition of J. thurifera EO extracted by hydrodistillation was analyzed by using the GC-MS technique. Antibacterial activity of EO alone and in combination with three conventional antibiotics was assessed by using disc diffusion method against four bacterial strains. Results: Thirty-five components were identified, representing ~87 % of the oil. The main components were m-mentha-6,8-diene (15.43 %), β-pinene (10.59 %), elemol (8.31 %) and terpinene-4-ol (7.44 %). The essential oil showed strong antibacterial activity against S. aureus and E. coli, but no activity against P. aeruginosa and B. subtilis. Synergistic effects were observed because of the combined application of EO with gentamicin against all strains tested, and with amoxicillin against B. subtilis. Furthermore, the combination of EO/cefazolin demonstrated an additive effect against B. subtilis. In contrast, the combination of EO with amoxicillin and céfazoline revealed antagonistic effects against S. aureus, E. coli, and P. aeruginosa. Conclusion: This is the first report on the chemical composition and antibacterial activity of Algerian juniper berries’ essential oil. The results indicate that the studied EO may be a promising source of antibacterial compounds that could be useful for pharmaceutical applications especially in combination with conventional antibiotics.

scholarly journals β-Lactamase inhibition profile of new amidine substituted diazabicyclooctanes

2020 ◽  
Author(s):  
zafar iqbal ◽  
Yuanyu Gao ◽  
Dong Tang ◽  
Xueqin Ma ◽  
Jinbo Ji ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Second Generation ◽  
Bacterial Species ◽  
The Other ◽  
Inhibition Activity ◽  
Bacterial Strains ◽  
E Coli ◽  
Mic Value

Diazabicyclooctane (DBO) scaffold is the backbone of non-β-lactam based second generation β-lactamase inhibitors. As part of our efforts we have synthesized a series of DBO derivatives A1-A23 containing amidine substituents at C2 position of the bicyclic ring. These compounds, alone and in combination with meropenem, were tested against ten bacterial strains for their antibacterial activity in vitro. All compounds didn’t show antibacterial activity when alone (MIC, >64 mg/L), however exhibited moderate inhibition activity in the presence of meropenem by lowering its MIC values. Compound A12 proved most potent among the other counterparts against all bacterial species with MIC from <0.125 mg/L – 2 mg/L, and is comparable to avibactam against both E. coli strains with MIC value of <0.125 mg/L.

scholarly journals Synthesis, Docking Studies, and In Vitro Evaluation of Some Novel Thienopyridines and Fused Thienopyridine–Quinolines as Antibacterial Agents and DNA Gyrase Inhibitors

Molecules ◽  
2019 ◽  
Vol 24 (20) ◽  
pp. 3650 ◽  
Author(s):  
Eman M. Mohi El-Deen ◽  
Eman A. Abd El-Meguid ◽  
Sherifa Hasabelnaby ◽  
Eman A. Karam ◽  
Eman S. Nossier
Keyword(s):  
Staphylococcus Aureus ◽  
Dna Gyrase ◽  
Docking Studies ◽  
Inhibition Activity ◽  
Bacterial Strains ◽  
Reference Drug ◽  
E Coli ◽  
Ic50 Values ◽  
Gyrase Inhibitors

A series of novel thienopyridines and pyridothienoquinolines (3a,b–14) was synthesized, starting with 2-thioxo-1,2-dihydropyridine-3-carbonitriles 1a and 1b. All compounds were evaluated for their in vitro antimicrobial activity against six bacterial strains. Compounds 3a,b, 4a, 5b, 6a,b, 7a, 9b, 12b, and 14 showed significant growth inhibition activity against both Gram-positive and Gram-negative bacteria compared with the reference drug. The most active compounds (4a, 7a, 9b, and 12b) against Staphylococcus aureus were also tested for their in vitro inhibitory action on methicillin-resistant Staphylococcus aureus (MRSA). The tested compounds showed promising inhibition activity, with the performance of 12b being equal to gentamicin and that of 7a exceeding it. Moreover, the most promising compounds were also screened for their Escherichia coli DNA gyrase inhibitory activity, compared with novobiocin as a reference DNA gyrase inhibitor. The results revealed that compounds (3a, 3b, 4a, 9b, and 12b) had the highest inhibitory capacity, with IC50 values of 2.26–5.87 µM (that of novobiocin is equal to 4.17 µM). Docking studies were performed to identify the mode of binding of the tested compounds to the active site of E. coli DNA gyrase B.

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