Factory trial: Dorr purification versus BMA65

Sugar Industry ◽

10.36961/si18650 ◽

2017 ◽

pp. 481-489

Author(s):

S. Royce ◽

D. Simkiss ◽

C.D. Rhoten ◽

R.W. Howe ◽

M.J. Blowers

Keyword(s):

Physical Properties ◽

Purification Process ◽

Industrial Trial ◽

Purification Methods

In terms of raw juice purification British Sugar uses Dorr carbonatation. However the classical purification process incorporates a ‘hot liming stage’ that results in juices of lower colour and increased juice thermostability. The downside of classical liming is a loss of ‘physical properties’ namely settlement and filtration. British Sugar has carried out an industrial trial that incorporates a ‘hot liming’ stage by adoption of the principles of BMA65. The trial was carried out at Bury St Edmunds factory during the 2016/17 campaign, the objective to directly compare the two purification methods. The paper discusses the results and observations made during the trial.

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A Study on the Purification methods of Carbon Nanotubes and Stm Imaging of the Carbon Nanotubes

MRS Proceedings ◽

10.1557/proc-349-231 ◽

1994 ◽

Vol 349 ◽

Cited By ~ 2

Author(s):

Motoo Yumura ◽

Kunio Uchida ◽

Hiroyuki Niino ◽

Satoshi Ohshima ◽

Yasunori Kuriki ◽

...

Keyword(s):

Carbon Nanotubes ◽

Raman Scattering ◽

Electronic Properties ◽

Amorphous Carbon ◽

Purification Process ◽

Graphitic Sheet ◽

Purification Methods ◽

Scattering Measurement

AbstractsPurification process to obtain nanotubes out of the cathode product mostly including fragments of graphitic sheet and/or amorphous carbon has been established. Structures and electronic properties of carbon nanotubes were studied. By the Raman scattering measurement, only one peak at 1581 cm−1 akin to that of graphite was found out. It was confirmed by STM observation of purified nanotubes. Purified nanotubes was semiconductor or semimetal.

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Process Development and Scale-up Optimization of the SARS-CoV-2 Receptor Binding Domain-Based Vaccine Candidate, RBD219-N1C1

10.1101/2020.12.30.424829 ◽

2020 ◽

Author(s):

Jungsoon Lee ◽

Zhuyun Liu ◽

Wen-Hsiang Chen ◽

Junfei Wei ◽

Rakhi Kundu ◽

...

Keyword(s):

Receptor Binding ◽

Process Development ◽

Low Cost ◽

Scale Up ◽

Neutralizing Antibody ◽

Protein Antigen ◽

Purification Process ◽

Good Manufacturing Practices ◽

Purification Methods ◽

Selection Of

AbstractA SARS-CoV-2 RBD219-N1C1 (RBD219-N1C1) recombinant protein antigen formulated on Alhydrogel® has recently been shown to elicit a robust neutralizing antibody response against SARS-CoV-2 pseudovirus in mice. The antigen has been produced under current good manufacturing practices (cGMP) and is now in clinical testing. Here, we report on process development and scale-up optimization for upstream fermentation and downstream purification of the antigen. This includes production at the 1 and 5 L scale in the yeast, Pichia pastoris, and the comparison of three different chromatographic purification methods. This culminated in the selection of a process to produce RBD219-N1C1 with a yield of >400 mg per liter of fermentation with >92% purity and >39% target product recovery after purification. In addition, we show the results from analytical studies, including SEC-HPLC, DLS, and an ACE2 receptor binding assay that were performed to characterize the purified proteins to select the best purification process. Finally, we propose an optimized upstream fermentation and downstream purification process that generates quality RBD219-N1C1 protein antigen and is fully scalable at a low cost.

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Process development and scale-up optimization of the SARS-CoV-2 receptor binding domain–based vaccine candidate, RBD219-N1C1

Applied Microbiology and Biotechnology ◽

10.1007/s00253-021-11281-3 ◽

2021 ◽

Author(s):

Jungsoon Lee ◽

Zhuyun Liu ◽

Wen-Hsiang Chen ◽

Junfei Wei ◽

Rakhi Kundu ◽

...

Keyword(s):

Receptor Binding ◽

Process Development ◽

Low Cost ◽

Scale Up ◽

Neutralizing Antibody ◽

Protein Antigen ◽

Vaccine Antigen ◽

Yeast Fermentation ◽

Purification Process ◽

Purification Methods

Abstract A SARS-CoV-2 RBD219-N1C1 (RBD219-N1C1) recombinant protein antigen formulated on Alhydrogel® has recently been shown to elicit a robust neutralizing antibody response against SARS-CoV-2 pseudovirus in mice. The antigen has been produced under current good manufacturing practices (cGMPs) and is now in clinical testing. Here, we report on process development and scale-up optimization for upstream fermentation and downstream purification of the antigen. This includes production at the 1-L and 5-L scales in the yeast, Pichia pastoris, and the comparison of three different chromatographic purification methods. This culminated in the selection of a process to produce RBD219-N1C1 with a yield of >400 mg per liter of fermentation with >92% purity and >39% target product recovery after purification. In addition, we show the results from analytical studies, including SEC-HPLC, DLS, and an ACE2 receptor binding assay that were performed to characterize the purified proteins to select the best purification process. Finally, we propose an optimized upstream fermentation and downstream purification process that generates quality RBD219-N1C1 protein antigen and is fully scalable at a low cost. Key points • Yeast fermentation conditions for a recombinant COVID-19 vaccine were determined. • Three purification protocols for a COVID-19 vaccine antigen were compared. • Reproducibility of a scalable, low-cost process for a COVID-19 vaccine was shown. Graphical abstract

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Quantification of anacardic acid, the toxic component in raw and purified samples of Semecarpus anacardium L. by Siddha purification processes

Journal of Complementary and Integrative Medicine ◽

10.1515/jcim-2021-0010 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Juliet Lawrence ◽

Reena Viswan Lilly ◽

Arthi Velmurugan ◽

Karthik Raja Sundhara moorthy ◽

Sudha Revathy Sudarsanam ◽

...

Keyword(s):

Defense Mechanisms ◽

Chemical Change ◽

Strong Support ◽

Anacardic Acid ◽

Purification Process ◽

Semecarpus Anacardium ◽

Purification Methods ◽

Moderate Change ◽

Physical And Chemical ◽

Major Reduction

Abstract Objectives The knowledge of purification process is unique since it deals with various physical and chemical changes in the inherent toxic properties during the process and it renders a strong support in the art of medicine preparation in Siddha system of medicine. Semecarpus anacardium L. is listed under Schedule e (1) drugs and purification is mandatory before its administration in medicine preparation. The bioactive compounds present in the plant like alkaloids, terpenoids, flavonoids, saponins, glycosides, steroids enhance the medical efficacy and defense mechanisms of the plant. Hence an attempt has been made to reveal the chemical change, occurring during the purification process in Siddha system of medicine by evaluating the phytochemicals and anacardic acid which is responsible for forming blisters and dermatitis. Methods Five common methods were used to purify the Semecarpus anacardium nuts as per the Siddha classical texts. The quantification of phytochemicals and anacardic acid has been done in unpurified, intermediate and purified samples. Results Significant changes were observed in phytochemicals and anacardic acid quantity in unpurified and purified samples. The major reduction of anacardic acid from 5.62 % in S1 to below detectable limit (BDL) in S2, S3, S4, 0.24% in S5 and 3.32% in S6. There is mild to moderate change in the phytochemicals which will support the efficacy of the drug. Conclusions With the results of phytochemicals, though all the purification methods have been evidenced to reduce the toxic nature, among the above five, the process II may be recommended for further research and medicine preparation.

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The Photometric Properties of the Ap Stars

International Astronomical Union Colloquium ◽

10.1017/s0252921100080684 ◽

1976 ◽

Vol 32 ◽

pp. 365-377 ◽

Cited By ~ 2

Author(s):

B. Hauck

Keyword(s):

Physical Properties ◽

Ap Stars

The Ap stars are numerous - the photometric systems tool It would be very tedious to review in detail all that which is in the literature concerning the photometry of the Ap stars. In my opinion it is necessary to examine the problem of the photometric properties of the Ap stars by considering first of all the possibility of deriving some physical properties for the Ap stars, or of detecting new ones. My talk today is prepared in this spirit. The classification by means of photoelectric photometric systems is at the present time very well established for many systems, such as UBV, uvbyβ, Vilnius, Geneva and DDO systems. Details and methods of classification can be found in Golay (1974) or in the proceedings of the Albany Colloquium edited by Philip and Hayes (1975).

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The Infection of Cells by Bullet-Shaped Viruses

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100063779 ◽

1969 ◽

Vol 27 ◽

pp. 372-373

Author(s):

Frederick A. Murphy ◽

Alyne K. Harrison ◽

Sylvia G. Whitfield

Keyword(s):

Electron Microscopy ◽

Physical Properties ◽

Host Cell ◽

Thin Section ◽

Cultured Cells ◽

Cell Infection ◽

Thin Section Electron Microscopy ◽

Section Electron ◽

Section Electron Microscopy

The bullet-shaped viruses are currently classified together on the basis of similarities in virion morphology and physical properties. Biologically and ecologically the member viruses are extremely diverse. In searching for further bases for making comparisons of these agents, the nature of host cell infection, both in vivo and in cultured cells, has been explored by thin-section electron microscopy.

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Transmission electron microscopy of composite materials

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100107125 ◽

1988 ◽

Vol 46 ◽

pp. 1012-1015

Author(s):

K.P.D. Lagerlof

Keyword(s):

Composite Materials ◽

Physical Properties ◽

Structural Defects ◽

Structural Composites ◽

Multiphase Materials ◽

Structural Reinforcement ◽

Transmission Electron ◽

Reinforced Fiber ◽

Particulate Reinforced Composites ◽

Definition Of

Although most materials contain more than one phase, and thus are multiphase materials, the definition of composite materials is commonly used to describe those materials containing more than one phase deliberately added to obtain certain desired physical properties. Composite materials are often classified according to their application, i.e. structural composites and electronic composites, but may also be classified according to the type of compounds making up the composite, i.e. metal/ceramic, ceramic/ceramie and metal/semiconductor composites. For structural composites it is also common to refer to the type of structural reinforcement; whisker-reinforced, fiber-reinforced, or particulate reinforced composites [1-4].For all types of composite materials, it is of fundamental importance to understand the relationship between the microstructure and the observed physical properties, and it is therefore vital to properly characterize the microstructure. The interfaces separating the different phases comprising the composite are of particular interest to understand. In structural composites the interface is often the weakest part, where fracture will nucleate, and in electronic composites structural defects at or near the interface will affect the critical electronic properties.

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