scholarly journals Chromosome Counting in Few Species of Musa by Traditional Technique

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
AL Veerabhadra Swamy ◽  
GV Amrutha
Keyword(s):  
Chromosome Counting ◽  
Traditional Technique

Optimization of FCAW Process Parameters using A Non-Traditional Technique

2008 ◽  
Vol 41 (3) ◽  
pp. 32
Author(s):  
T. Kannan ◽  
N. Murugan ◽  
N. A. Prabu
Keyword(s):  
Process Parameters ◽  
Traditional Technique

Antibiotic Distribution in a Bone Cement Spacer Model

2007 ◽  
Vol 17 (4) ◽  
pp. 218-223 ◽  
Author(s):  
K. Shiramizu ◽  
V. Lovric ◽  
A.M.D. Leung ◽  
W.R. Walsh
Keyword(s):  
Liquid Phase ◽  
Bone Cement ◽  
Cross Sections ◽  
High Dose ◽  
Cement Spacer ◽  
Traditional Technique ◽  
Low Viscosity ◽  
Significant Difference ◽  
Group A ◽  
Medium Viscosity

Purpose To mix high dose antibiotic powder to the bone cement more easily, Hanssen et al reported mixing the antibiotics with the cement during its liquid phase but made no comments about the relevance of cement viscosity and antibiotic distribution. The purpose of this study was to investigate the effect of the cement mixing technique and cement viscosity on the antibiotics distribution in a cement spacer model. Methods Thirty cylindrical models from three groups were examined. Group A was made by mixing the antibiotics with medium viscosity cement prior to adding the liquid monomer (traditional technique). Group B was made by mixing the antibiotics with medium viscosity cement during its liquid phase (Hanssen's technique). Group C was made by traditional technique with low viscosity cement. In all groups 2 g of tetracycline was used. Three 0.1 mm thick cross sections from each spacer model were examined under the fluorescent microscope. The fluorescent spots of tetracycline were calculated automatically in pixels. To evaluate the distribution of the antibiotics in the spacer model, we selected the cross section with the highest number of pixels and the one with the lowest number of pixels from each of the three cross sections and calculated the difference between them. The distribution disequilibrium was compared between group A and B, A and C. Results No significant difference was observed in either comparison. Conclusion The Hanssen's mixing technique can be used when using high dose antibiotics, and either medium or low viscosity cement could be used in terms of antibiotic distribution.

scholarly journals Needle Adjustment Free (NAF) running suture technique (PAN suture) in laparoscopic partial nephrectomy

BMC Surgery ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jun-wei Pan ◽  
Xiang Zhang ◽  
Xing-wei Jin ◽  
Xiao Liu ◽  
Wei-chao Tu ◽  
...  
Keyword(s):  
Partial Nephrectomy ◽  
Conversion Rate ◽  
Suture Technique ◽  
Laparoscopic Partial Nephrectomy ◽  
Continuous Suture ◽  
Discharge Time ◽  
Open Conversion ◽  
Traditional Technique ◽  
Affected Side ◽  
Suture Group

Abstract Background It is proposed a new running suture technique called Needle Adjustment Free (NAF) technique, or PAN suture. The efficiency and the safety were evaluated in laparoscopic partial nephrectomy. Methods This new running suture technique avoids the Needle Adjustment method used in traditional techniques. The new continuous suture technique (11 patients) was compared with the traditional continuous suture method (33 patients) used in both transperitoneal and retroperitoneal laparoscopic partial nephrectomy (LPN) in terms of suture time (ST), warm ischemia time (WIT), blood loss (BL), open conversion rate and post-op discharge time, post-op bleeding, post-op DVT, ΔGFR (affected side, 3 months post-op). Differences were considered significant when P < 0.05. Results ST in the PAN suture group was 30.37 ± 16.39 min, which was significant shorter (P = 0.0011) than in the traditional technique group which was 13.68 ± 3.33 min. WIT in the traditional technique group was 28.73 ± 7.89 min, while in the PAN suture group was 20.64 ± 5.04 min, P = 0.0028. The BL in entirety in the traditional technique group was 141.56 ± 155.23 mL, and in the PAN suture group was 43.18 ± 31.17 mL (P = 0.0017). BL in patients without massive bleeding in the traditional technique group was significantly greater than in the PAN suture group at 101.03 ± 68.73 mL versus 43.18 ± 31.17 mL (P = 0.0008). The open conversion rate was 0 % in both groups. There was no significant difference between the two groups in postoperative discharge time, post-op bleeding, post-op DVT, ΔGFR (affected side, 3 months post-op). Conclusions The NAF running suture technique, or PAN suture, leading to less ST, WIT and BL, which was shown to be more effective and safer than the traditional technique used for LPN. A further expanded research with larger sample size is needed.

scholarly journals Studies on Colchicine Induced Chromosome Doubling for Enhancement of Quality Traits in Ornamental Plants

Plants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 194 ◽  
Author(s):  
Ayesha Manzoor ◽  
Touqeer Ahmad ◽  
Muhammad Bashir ◽  
Ishfaq Hafiz ◽  
Cristian Silvestri
Keyword(s):  
Chromosome Doubling ◽  
Ornamental Plants ◽  
Plant Size ◽  
Unreduced Gametes ◽  
Chromosome Counting ◽  
Morphological And Physiological Traits ◽  
Routine Procedures ◽  
Mitotic Inhibitor ◽  
Intense Color

Polyploidy has the utmost importance in horticulture for the development of new ornamental varieties with desirable morphological traits referring to plant size and vigor, leaf thickness, larger flowers with thicker petals, intense color of leaves and flowers, long lasting flowers, compactness, dwarfness and restored fertility. Polyploidy may occur naturally due to the formation of unreduced gametes or can be artificially induced by doubling the number of chromosomes in somatic cells. Usually, natural polyploid plants are unavailable, so polyploidy is induced synthetically with the help of mitotic inhibitors. Colchicine is a widely used mitotic inhibitor for the induction of polyploidy in plants during their cell division by inhibiting the chromosome segregation. Different plant organs like seeds, apical meristems, flower buds, and roots can be used to induce polyploidy through many application methods such as dipping/soaking, dropping or cotton wool. Flow cytometry and chromosome counting, with an observation of morphological and physiological traits are routine procedures for the determination of ploidy level in plants.

Optimization of ultraviolet irradiation of Clarias gariepinus (Burchell, 1822) eggs for androgen production

Zygote ◽  
2021 ◽  
pp. 1-6
Author(s):  
Victor Tosin Okomoda ◽  
Haziqah Jumahat Nursyaza ◽  
Ijabo Oga Samuel ◽  
Anuar Hassan ◽  
Abraham Sunday Oladimeji ◽  
...  
Keyword(s):  
Survival Rate ◽  
Uv Irradiation ◽  
Uv Light ◽  
Clarias Gariepinus ◽  
African Catfish ◽  
Chromosome Counting ◽  
Complete Inactivation ◽  
Cell Cleavage ◽  
Optimal Protocol ◽  
Prolonged Duration

Summary The optimum distance and duration of ultraviolet (UV) irradiation for the complete inactivation of African catfish Clarias gariepinus egg nucleus was investigated in this study. The UV light was suspended above the unfertilized eggs at four distances (5, 10, 20 and 30 cm) and for five durations (1, 2, 3, 4 and 5 min). Then, the irradiated eggs were activated with sperm from diploid C. gariepinus and cold shocked at 5°C for 5 min just moments before cell cleavage. Ploidy analysis was performed using karyotype chromosome counting. The results obtained suggested that the further the distance, the better the hatchability rate, however prolonged duration seemed to significantly reduced hatchability. All treatments with surviving progenies at the end of the study showed evidence of successfully diploid gynogen (2n = 56) induction at different percentages. However, the optimal protocol that gave a moderately high hatchability/survival rate and completely induced gynogens was exposure of the eggs to UV irradiation at 20 cm for 1 min. It was concluded that the distance and duration of UV irradiation affects gynogenetic induction in African catfish C. gariepinus.

Regulation of X-Chromosome Counting by Tsix and Xite Sequences

Science ◽  
2005 ◽  
Vol 309 (5735) ◽  
pp. 768-771 ◽  
Author(s):  
J. T. Lee
Keyword(s):  
X Chromosome ◽  
Chromosome Counting

scholarly journals Mechanisms involved in the induction of aneuploidy: the significance of chromosome loss

2000 ◽  
Vol 23 (4) ◽  
pp. 1077-1082 ◽  
Author(s):  
A.I. Seoane ◽  
A.M. Güerci ◽  
F.N. Dulout
Keyword(s):  
Cadmium Chloride ◽  
Potassium Dichromate ◽  
Chinese Hamster ◽  
Nickel Chloride ◽  
Chromosome Loss ◽  
X Rays ◽  
Chromosome Counting ◽  
Cadmium Sulfate ◽  
Chromium Chloride ◽  
Diploid Cells

The induction of aneuploidy by physical and chemical agents using different test systems was evaluated. The effect of X-rays, caffeine, acetaldehyde, ethanol, diethylstilbestrol, propionaldehyde, and chloral hydrate was studied by chromosome counting in Chinese hamster embryonic diploid cells. Aneugenic ability of cadmium chloride, cadmium sulfate, potassium dichromate, chromium chloride, nickel chloride, and nickel sulfate was assessed by means of anaphase-telophase analysis in Chinese hamster ovary cells. Chromosome counting in human fibroblasts (MRC-5 cell line) was employed to evaluate the effect of cacodilic acid, cadmium chloride, cadmium sulfate, and potassium dichromate. Finally, the induction of kinetochore-positive and kinetochore negative micronuclei by cadmium chloride, cadmium sulfate, potassium dichromate, chromium chloride, and nickel chloride was studied using CREST antibodies. When the effect of different agents was determined by chromosome counting, an increase of hypoploid but not of hyperploid cells was observed. Anaphase-telophase analysis showed that metal salts increased the frequency of lagging chromosomes. This finding has been confirmed by the increment of kinetochore-positive micronuclei using CREST antibodies. Therefore, chromosome loss could be considered as the main cause of induced aneuploidy.

TACE with cone beam computed tomography is more effective than traditional technique in BCLC A HCC patients ineligible to surgery

2015 ◽  
Vol 47 ◽  
pp. e33-e34
Author(s):  
R. Patti ◽  
N. Mezzina ◽  
F. Masutti ◽  
C. Abazia ◽  
V. Lanzillotti ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Cone Beam Computed Tomography ◽  
Cone Beam ◽  
Traditional Technique
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