scholarly journals A Nasopharyngeal Swab Specimen: How to Perform?

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Şahin Bayram ◽  
Aytuluk Hande Gürbüz
Keyword(s):  
Nasopharyngeal Swab ◽  
Swab Specimen

scholarly journals Is the patient infected with SARS-CoV-2?

2021 ◽  
Author(s):  
Jeffrey D Klausner ◽  
Noah Kojima ◽  
Susan M. Butler-Wu
Keyword(s):  
Drug Administration ◽  
Food And Drug Administration ◽  
Nasopharyngeal Swab ◽  
Reference Standard ◽  
The Us ◽  
Swab Specimen

The US Food and Drug Administration currently uses the nasopharyngeal swab specimen as the reference standard for evaluation of SARS-CoV-2 assays. We propose that the patient-infected status algorithm is a superior way to classify whether an individual is infected or not infected.

scholarly journals Para-aortic lymphadenopathy associated with adult COVID-19 multisystem inflammatory syndrome

2021 ◽  
Vol 14 (12) ◽  
pp. e246884
Author(s):  
Victor Carvalho ◽  
Paula H Damasco ◽  
Thiago S Mello ◽  
Bruno Gonçalves
Keyword(s):  
Complete Resolution ◽  
High Flow ◽  
Nasopharyngeal Swab ◽  
Minimal Amount ◽  
High Flow Nasal Cannula ◽  
Reverse Transcription Pcr ◽  
Aortic Lymph Node ◽  
Inflammatory Syndrome ◽  
Swab Specimen ◽  
Interlobular Septal Thickening

A 21-year-old woman arrived at the emergency department with dyspnoea, arterial hypotension and abdominal pain after 5 days with a influenza-like syndrome. SARS-CoV-2 was detected by reverse transcription PCR in a nasopharyngeal swab specimen. CT of the chest and abdomen with contrast demonstrated a minimal amount of free intraperitoneal fluid, gallbladder with wall oedema, multiple para-aortic lymph node and interlobular septal thickening with ground glass opacities on the lungs. No pleural effusion or thromboembolism. Early broad-spectrum antibiotics, high-flow nasal cannula and norepinephrine were started. She was successfully treated with intravenous immunoglobulin and pulse corticosteroid therapy with methylprednisolone. The patient was discharged home with complete resolution of her symptoms and returned to her previous health status.

scholarly journals How to Obtain a Nasopharyngeal Swab Specimen

2020 ◽  
Vol 382 (22) ◽  
pp. e76 ◽  
Author(s):  
Francisco M. Marty ◽  
Kaiwen Chen ◽  
Kelly A. Verrill
Keyword(s):  
Nasopharyngeal Swab ◽  
Swab Specimen

scholarly journals Comparison of Two Commercial Molecular Tests and a Laboratory-Developed Modification of the CDC 2019-nCoV Reverse Transcriptase PCR Assay for the Detection of SARS-CoV-2

2020 ◽  
Vol 58 (8) ◽  
Author(s):  
Nicholas M. Moore ◽  
Haiying Li ◽  
Debra Schejbal ◽  
Jennifer Lindsley ◽  
Mary K. Hayden
Keyword(s):  
Reverse Transcriptase ◽  
Medical Record ◽  
Medical Record Review ◽  
Nasopharyngeal Swab ◽  
Upper Respiratory Tract ◽  
Pcr Assay ◽  
Reverse Transcriptase Pcr ◽  
Viral Transport Medium ◽  
Record Review ◽  
Swab Specimen

ABSTRACT We compared the ability of 2 commercial molecular amplification assays (RealTime SARS-CoV-2 on the m2000 [abbreviated ACOV; Abbott] and ID Now COVID-19 [abbreviated IDNOW; Abbott]) and a laboratory-developed test (modified CDC 2019-nCoV reverse transcriptase PCR [RT-PCR] assay with RNA extraction by eMag [bioMérieux] and amplification on QuantStudio 6 or ABI 7500 real-time PCR system [abbreviated CDC COV]) to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in upper respiratory tract specimens. Discrepant results were adjudicated by medical record review. A total of 200 nasopharyngeal swab specimens in viral transport medium (VTM) were collected from symptomatic patients between 27 March and 9 April 2020. Results were concordant for 167 specimens (83.5% overall agreement), including 94 positive and 73 negative specimens. The ACOV assay yielded 33 additional positive results, 25 of which were also positive by the CDC COV assay but not by the IDNOW assay. In a follow-up evaluation, 97 patients for whom a dry nasal swab specimen yielded negative results by IDNOW had a paired nasopharyngeal swab specimen collected in VTM and tested by the ACOV assay; SARS-CoV-2 RNA was detected in 13 (13.4%) of these specimens. Medical record review deemed all discrepant results to be true positives. The IDNOW test was the easiest to perform and provided a result in the shortest time but detected fewer cases of COVID-19. The ACOV assay detected more cases of COVID-19 than the CDC COV or IDNOW assays.

scholarly journals Complete Genome Sequence of a 2019 Novel Coronavirus (SARS-CoV-2) Strain Causing a COVID-19 Case in Morocco

2020 ◽  
Vol 9 (27) ◽  
Author(s):  
Sanaâ Lemriss ◽  
Amal Souiri ◽  
Narjis Amar ◽  
Nabil Lemzaoui ◽  
Omar Mestoui ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Nasopharyngeal Swab ◽  
Novel Coronavirus ◽  
Swab Specimen ◽  
Moroccan Patient

ABSTRACT Here, we report a complete genome sequence obtained for a novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strain isolated from a nasopharyngeal swab specimen of a Moroccan patient with coronavirus disease 2019 (COVID-19).

scholarly journals A Novel Just-In-Time-Online-Training for Nasopharyngeal Swab Specimen Collection During the COVID-19 Pandemic

Cureus ◽  
2021 ◽  
Author(s):  
Asit Misra ◽  
Kristy J Carlson ◽  
Christie A Barnes ◽  
Samuel K Pate ◽  
Benjamin B Stobbe ◽  
...  
Keyword(s):  
Online Training ◽  
Just In Time ◽  
Nasopharyngeal Swab ◽  
Specimen Collection ◽  
Swab Specimen

scholarly journals Frequency of asymptomatic carriers of SARS-CoV-2 among children and adults after school reopening

2021 ◽  
Vol 47 (1) ◽  
Author(s):  
Gregorio P. Milani ◽  
Paola Marchisio ◽  
Alessia Rocchi ◽  
Giuseppe Bertolozzi ◽  
Ludovico Furlan ◽  
...  
Keyword(s):  
Odds Ratio ◽  
Cross Sectional Study ◽  
After School ◽  
Nasopharyngeal Swab ◽  
Short Stay ◽  
Sectional Study ◽  
Cross Sectional ◽  
Similar Frequency ◽  
Asymptomatic Carriers ◽  
Swab Specimen

Abstract Background Children often develop an asymptomatic form of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), but it is debated if children are at higher risk than adults to be asymptomatic carriers of SARS-CoV-2, especially during the school reopening. The main aim of this study was to investigate the frequency of SARS-CoV-2 asymptomatic carriers in children and adults during the reopening of the schools in Milan, Italy. Methods We conducted a cross-sectional study at the pediatric and adult Emergency Department (ED) of the Ca' Granda Ospedale Maggiore Policlinico (Milan) between October 1 and 31, 2020, i.e. 3 weeks after the reopening of schools. Patients admitted to the ED short stay observation and without any sign or symptom consistent with a SARS-CoV-2 were eligible. These patients underwent a nasopharyngeal swab specimen for the detection of SARS-CoV-2. The odds ratio and its 95% confidence interval (CI) was calculated to assess the risk of asymptomatically carrying the SARS-CoV-2 infection in children and adults. Results A total of 69 (27 females, median age 8.7 years) children and 251 (107 females, median age 71 years) adults were enrolled. Pediatric and adult subjects tested positive for SARS-CoV-2 with a similar frequency (1/69 [1.4%] vs 4/251 [1.6%]). Children had an odds ratio to be a carrier of 0.91 (CI 0.02– 9.38) compared to adults. Conclusions The frequency of asymptomatic SARS-CoV-2 carriers was similar among children and adults. Considering the emerging diffusion of new SARS-CoV-2 variants, the asymptomatic spread of SARS-CoV-2 infection among children and adults should be monitored.

scholarly journals First Report of a SARS-CoV-2 Genome Sequence with a Spike His69-Val70 Deletion and an Asn439Lys Mutation in Morocco

2021 ◽  
Vol 10 (11) ◽  
Author(s):  
Tarik Aanniz ◽  
Mouna Ouadghiri ◽  
Mohammed Amine Bendahou ◽  
Mohammed Walid Chemao-Elfihri ◽  
Mohamed Chenaoui ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Male Patient ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Clinical Sample ◽  
Nasopharyngeal Swab ◽  
Ion Torrent ◽  
First Report ◽  
Swab Specimen

ABSTRACT We report the nearly complete genome sequence and the genetic variations of a clinical sample of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) collected from a nasopharyngeal swab specimen from a male patient from Harhoura-Rabat, Morocco. The sequence, which was obtained using Ion Torrent technology, is valuable as it carries a recently described deletion (His69-Val70) and substitution (Asn439Lys).

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