"Determining Whether Julius Seizure Protein Partners Skap, Nrv3, and ATPα-6a Affect Bang- and Cold-Sensitivity in Drosophila melanogaster"

2021 ◽  
Author(s):  
Juan Peticco
Keyword(s):  
Drosophila Melanogaster ◽  
Cold Sensitivity ◽  
Protein Partners

scholarly journals TEMPERATURE-SENSITIVE MUTATIONS IN DROSOPHILA MELANOGASTER. XVII. HEAT- AND COLD-SENSITIVE LETHALS ON CHROMOSOME 3

Genetics ◽  
1973 ◽  
Vol 74 (3) ◽  
pp. 509-520
Author(s):  
S Elaine Tasaka ◽  
David T Suzuki
Keyword(s):  
Drosophila Melanogaster ◽  
Ethyl Methanesulfonate ◽  
Cold Sensitivity ◽  
Chromosome 3 ◽  
Temperature Sensitive ◽  
Single Mutation ◽  
Physical Length ◽  
Lethal Mutations ◽  
Genetic Length ◽  
Cold Sensitive

ABSTRACT Ethyl methanesulfonate-treated third chromosome of Drosophila melanogaster were tested for the presence of dominant and recessive temperature-sensitive lethal mutations at 17°, 22° and 29°C. Out of 1,176 chromosomes tested, no dominant ts lethals, 21 heat-sensitive, 22 cold-sensitive and 10 heat-cold-sensitive lethals were recovered. Heat-cold sensitivity was produced by a single mutation in all cases. Sixty-two percent of the ts lethals were fertile as homozygotes in both sexes. Surprisingly, 88% of the ts lethals mapped between st and Sb, a region straddling the centromere and estimated to comprise 12.9% of the genetic length and 55% of the physical length of chromosome 3. All but one of the heat- and cold-sensitive lethals complemented with each other at their respective restrictive temperatures.

scholarly journals The lethal(1)TW-6cs mutation of Drosophila melanogaster is a dominant antimorphic allele of nod and is associated with a single base change in the putative ATP-binding domain.

Genetics ◽  
1991 ◽  
Vol 129 (2) ◽  
pp. 409-422 ◽  
Author(s):  
R S Rasooly ◽  
C M New ◽  
P Zhang ◽  
R S Hawley ◽  
B S Baker
Keyword(s):  
Drosophila Melanogaster ◽  
Chromosome Breakage ◽  
Base Change ◽  
Cold Sensitivity ◽  
Atp Binding ◽  
Loss Of Function ◽  
Single Base ◽  
Recessive Lethal Mutation ◽  
Cold Sensitive ◽  
Single Base Change

Abstract The l(1)TW-6cs mutation is a cold-sensitive recessive lethal mutation in Drosophila melanogaster, that affects both meiotic and mitotic chromosome segregation. We report the isolation of three revertants of this mutation. All three revert both the meiotic and mitotic effects as well as the cold sensitivity, demonstrating that all three phenotypes are due to a single lesion. We further show that these revertants fail to complement an amorphic allele of the nod (no distributive disjunction) locus, which encodes a kinesin-like protein. These experiments demonstrate that l(1)TW-6cs is an antimorphic allele of nod, and we rename it nodDTW. Sequencing of the nod locus on a nodDTW-bearing chromosome reveals a single base change in the putative ATP-binding region of the motor domain of nod. Recessive, loss-of-function mutations at the nod locus specifically disrupt the segregation of nonexchange chromosomes in female meiosis. We demonstrate that, at 23.5 degrees, the meiotic defects in nodDTW/+ females are similar to those observed in nod/nod females; that is, the segregation of nonexchange chromosomes is abnormal. However, in nodDTW/nodDTW females, or in nodDTW/+ females at 18 degrees, we observe a more severe meiotic defect that apparently affects the segregation of both exchange and nonexchange chromosomes. In addition, nodDTW homozygotes and hemizygous males have previously been shown to exhibit mitotic defects including somatic chromosome breakage and loss. We propose that the defective protein encoded by the nodDTW allele interferes with proper chromosome movement during both meiosis and mitosis, perhaps by binding irreversibly to microtubules.

scholarly journals The evolution of cold nociception in drosophilid larvae and identification of a neural basis for cold acclimation

2021 ◽  
Author(s):  
Nathaniel J. Himmel ◽  
Jamin M. Letcher ◽  
Akira Sakurai ◽  
Thomas R. Gray ◽  
Maggie N. Benson ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Cold Acclimation ◽  
Cold Tolerance ◽  
Cold Sensitivity ◽  
Class Iii ◽  
Neural Basis ◽  
Body Of Knowledge ◽  
Growing Body ◽  
Noxious Cold ◽  
Cold Sensing

AbstractCold temperatures can be fatal to insects, but many species have evolved the ability to cold acclimate, thereby increasing their cold tolerance. While there is a growing body of knowledge concerning the mechanisms underlying cold tolerance, relatively little is known concerning how insects sense noxious cold (cold nociception), or how cold nociception might function in cold tolerance. It has been previously shown that Drosophila melanogaster larvae perform highly stereotyped, cold-evoked behaviors under the control of noxious cold-sensing neurons (nociceptors) innervating the barrier epidermis. In the present study, we first sought to describe cold-nociceptive behavior among 11 drosophilid species with differing cold tolerances and from differing climates. Behavioral analyses revealed that the predominant cold-evoked response among drosophilid larvae is a head-to-tail contraction (CT) behavior, which is likely inherited from a common ancestor. However, despite lack of phylogenetic signal (suggesting trait lability), the CT behavior was transient and there was no clear evidence that cold sensitivity was related to thermal environment; collectively this suggests that the behavior might not be adaptive. We therefore sought to uncover an alternative way that cold nociception might be protective. Using a combination of cold-shock assays, optogenetics, electrophysiology, and methods to genetically disrupt neural transmission, we demonstrate that cold sensing neurons in Drosophila melanogaster (Class III nociceptors) are sensitized by and critical to cold acclimation. Moreover, we demonstrate that cold acclimation can be optogenetically-evoked, sans cold. Collectively, these findings reveal that cold nociception constitutes a peripheral neural basis for Drosophila larval cold acclimation.Significance StatementMany insects adapt to cold in response to developmental exposure to cool temperatures. While there is a growing body of knowledge concerning the mechanisms underlying cold tolerance, it is unknown how sensory neurons might contribute. Here, we show that noxious cold sensing (cold nociception) is widely present among drosophilid larvae, and that cold-sensing neurons (Class III cold nociceptors) are necessary and sufficient drivers of cold acclimation. This suggests that cold acclimation has, at least in part, a neural basis.

TEMPERATURE-SENSITIVE MUTATIONS IN DROSOPHILA MELANOGASTER. XVI. THE GENETIC PROPERTIES OF SEX-LINKED RECESSIVE COLD-SENSITIVE MUTANTS

1973 ◽  
Vol 15 (2) ◽  
pp. 237-254 ◽  
Author(s):  
Helen Mayoh ◽  
David T. Suzuki
Keyword(s):  
Drosophila Melanogaster ◽  
Ribosomal Protein ◽  
Cold Sensitivity ◽  
Female Sterility ◽  
Temperature Sensitive ◽  
X Chromosomes ◽  
Cold Sensitive ◽  
The Right ◽  
Minute Mutations

Among 3,919 EMS-treated X chromosomes, 25 were retained as cold-sensitive (cs) lethals. That is, more than 20% of flies carrying a cs lethal survive at 22 °C and none at 17 °C and for cs semi-lethals, >30% at 22 °C and <13% at 17 °C. The cs mutations are not randomly distributed, seven being located at the X tip and three being alleles to the right of car. Over half exhibited female sterility or reduced fertility and seven exhibited visible phenotypes characteristic of bobbed and Minute mutations. The possible enrichment for ribosomal protein defects by screening for cold-sensitivity is discussed.

scholarly journals Proteomic Characterization of Inbreeding-Related Cold Sensitivity in Drosophila melanogaster

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e62680 ◽  
Author(s):  
Cornelis J. Vermeulen ◽  
Kamilla S. Pedersen ◽  
Hans C. Beck ◽  
Jørgen Petersen ◽  
Kristina Kirilova Gagalova ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Cold Sensitivity

Author response for "Location and arrangement of campaniform sensilla in Drosophila melanogaster"

2020 ◽  
Author(s):  
Gesa F. Dinges ◽  
Alexander S. Chockley ◽  
Till Bockemühl ◽  
Kei Ito ◽  
Alexander Blanke ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Author Response ◽  
Campaniform Sensilla

Live Confocal Analysis of Fertilization and Early Development

2001 ◽  
Vol 7 (S2) ◽  
pp. 1012-1013
Author(s):  
Uyen Tram ◽  
William Sullivan
Keyword(s):  
Drosophila Melanogaster ◽  
Embryonic Development ◽  
Real Time ◽  
Early Development ◽  
Fluorescent Probes ◽  
Primary Defect ◽  
Fluorescent Analysis ◽  
Dynamic Event ◽  
Enormous Amount ◽  
Fluorescent Studies

Embryonic development is a dynamic event and is best studied in live animals in real time. Much of our knowledge of the early events of embryogenesis, however, comes from immunofluourescent analysis of fixed embryos. While these studies provide an enormous amount of information about the organization of different structures during development, they can give only a static glimpse of a very dynamic event. More recently real-time fluorescent studies of living embryos have become much more routine and have given new insights to how different structures and organelles (chromosomes, centrosomes, cytoskeleton, etc.) are coordinately regulated. This is in large part due to the development of commercially available fluorescent probes, GFP technology, and newly developed sensitive fluorescent microscopes. For example, live confocal fluorescent analysis proved essential in determining the primary defect in mutations that disrupt early nuclear divisions in Drosophila melanogaster. For organisms in which GPF transgenics is not available, fluorescent probes that label DNA, microtubules, and actin are available for microinjection.

Apoptosis and development

2003 ◽  
Vol 39 ◽  
pp. 11-24 ◽  
Author(s):  
Justin V McCarthy
Keyword(s):  
Drosophila Melanogaster ◽  
Mammalian Cells ◽  
Cell Number ◽  
Model Organisms ◽  
Biological Processes ◽  
Nematode Caenorhabditis Elegans ◽  
Apoptotic Pathway ◽  
Genetic Pathways ◽  
Evolutionarily Conserved ◽  
Multicellular Organisms

Apoptosis is an evolutionarily conserved process used by multicellular organisms to developmentally regulate cell number or to eliminate cells that are potentially detrimental to the organism. The large diversity of regulators of apoptosis in mammalian cells and their numerous interactions complicate the analysis of their individual functions, particularly in development. The remarkable conservation of apoptotic mechanisms across species has allowed the genetic pathways of apoptosis determined in lower species, such as the nematode Caenorhabditis elegans and the fruitfly Drosophila melanogaster, to act as models for understanding the biology of apoptosis in mammalian cells. Though many components of the apoptotic pathway are conserved between species, the use of additional model organisms has revealed several important differences and supports the use of model organisms in deciphering complex biological processes such as apoptosis.

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