Defining the promoter architecture of the SCO4409 sigma factor gene in Streptomyces coelicolor

2020 ◽  
Author(s):  
Anastasia Tishena
Keyword(s):  
Sigma Factor ◽  
Streptomyces Coelicolor ◽  
Factor Gene ◽  
Promoter Architecture

scholarly journals Construction and Characterization of aMycobacterium tuberculosis Mutant Lacking the Alternate Sigma Factor Gene, sigF

2000 ◽  
Vol 68 (10) ◽  
pp. 5575-5580 ◽  
Author(s):  
Ping Chen ◽  
Rafael E. Ruiz ◽  
Qing Li ◽  
Richard F. Silver ◽  
William R. Bishai
Keyword(s):  
Stationary Phase ◽  
Sigma Factor ◽  
Type Strain ◽  
Wild Type Strain ◽  
Axenic Culture ◽  
Wild Type ◽  
Intracellular Growth ◽  
Factor Gene ◽  
Time To Death

ABSTRACT The alternate RNA polymerase sigma factor gene, sigF, which is expressed in stationary phase and under stress conditions in vitro, has been deleted in the virulent CDC1551 strain ofMycobacterium tuberculosis. The growth rate of the ΔsigF mutant was identical to that of the isogenic wild-type strain in exponential phase, although in stationary phase the mutant achieved a higher density than the wild type. The mutant showed increased susceptibility to rifampin and rifapentine. Additionally, the ΔsigF mutant displayed diminished uptake of chenodeoxycholate, and this effect was reversed by complementation with a wild-type sigF gene. No differences in short-term intracellular growth between mutant and wild-type organisms within human monocytes were observed. Similarly, the organisms did not differ in their susceptibilities to lymphocyte-mediated inhibition of intracellular growth. However, mice infected with the ΔsigF mutant showed a median time to death of 246 days compared with 161 days for wild-type strain-infected animals (P < 0.001). These data indicate that M. tuberculosis sigF is a nonessential alternate sigma factor both in axenic culture and for survival in macrophages in vitro. While the ΔsigF mutant produces a lethal infection of mice, it is less virulent than its wild-type counterpart by time-to-death analysis.

scholarly journals Expression of a Cryptic Secondary Sigma Factor Gene Unveils Natural Competence for DNA Transformation in Staphylococcus aureus

2012 ◽  
Vol 8 (11) ◽  
pp. e1003003 ◽  
Author(s):  
Kazuya Morikawa ◽  
Aya J. Takemura ◽  
Yumiko Inose ◽  
Melody Tsai ◽  
Le Thuy Nguyen Thi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Sigma Factor ◽  
Dna Transformation ◽  
Natural Competence ◽  
Factor Gene

Pleiotropic anti-anti-sigma factor BldG is phosphorylated by several anti-sigma factor kinases in the process of activating multiple sigma factors in Streptomyces coelicolor A3(2)

Gene ◽  
2020 ◽  
Vol 755 ◽  
pp. 144883
Author(s):  
Beatrica Sevcikova ◽  
Bronislava Rezuchova ◽  
Erik Mingyar ◽  
Dagmar Homerova ◽  
Renata Novakova ◽  
...  
Keyword(s):  
Sigma Factor ◽  
Streptomyces Coelicolor ◽  
Sigma Factors

scholarly journals DNA base sequence variability inkatF(putative sigma factor) gene ofEscherichia coli

1992 ◽  
Vol 20 (20) ◽  
pp. 5479-5480 ◽  
Author(s):  
Anna Ivanova ◽  
Mary Renshaw ◽  
Ramareddy V. Guntaka ◽  
Gerald Cohen
Keyword(s):  
Sigma Factor ◽  
Ofescherichia Coli ◽  
Base Sequence ◽  
Sequence Variability ◽  
Factor Gene ◽  
Dna Base

Conservation of Putative Promoter Sequences Located Upstream of Chlamydial Major Sigma Factor Gene,sigAamongChlamydiaSpp.

1999 ◽  
Vol 43 (5) ◽  
pp. 419-424 ◽  
Author(s):  
Yoshitsugu Ochiai ◽  
Hideto Fukushi ◽  
Yan Cai ◽  
Tsuyoshi Yamaguchi ◽  
Katsuya Hirai
Keyword(s):  
Sigma Factor ◽  
Putative Promoter ◽  
Factor Gene ◽  
Promoter Sequences

scholarly journals BldG and SCO3548 Interact Antagonistically To Control Key Developmental Processes in Streptomyces coelicolor

2009 ◽  
Vol 191 (8) ◽  
pp. 2541-2550 ◽  
Author(s):  
Archana Parashar ◽  
Kimberley R. Colvin ◽  
Dawn R. D. Bignell ◽  
Brenda K. Leskiw
Keyword(s):  
Sigma Factor ◽  
Antibiotic Production ◽  
Streptomyces Coelicolor ◽  
Affinity Purification ◽  
Regulatory System ◽  
Direct Interaction ◽  
Morphological Differentiation ◽  
Sigma Factors ◽  
Developmental Processes ◽  
Two Hybrid

ABSTRACT The similarity of BldG and the downstream coexpressed protein SCO3548 to anti-anti-sigma and anti-sigma factors, respectively, together with the phenotype of a bldG mutant, suggests that BldG and SCO3548 interact as part of a regulatory system to control both antibiotic production and morphological differentiation in Streptomyces coelicolor. A combination of bacterial two-hybrid, affinity purification, and far-Western analyses demonstrated that there was self-interaction of both BldG and SCO3548, as well as a direct interaction between the two proteins. Furthermore, a genetic complementation experiment demonstrated that SCO3548 antagonizes the function of BldG, similar to other anti-anti-sigma/anti-sigma factor pairs. It is therefore proposed that BldG and SCO3548 form a partner-switching pair that regulates the function of one or more sigma factors in S. coelicolor. The conservation of bldG and sco3548 in other streptomycetes demonstrates that this system is likely a key regulatory switch controlling developmental processes throughout the genus Streptomyces.

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