scholarly journals Differential screening of a subtractive cDNA library reveals that maternal undernutrition affects fetal heart gene expression

2005 ◽  
pp. 28-29
Author(s):  
Hyungchul Han ◽  
Thomas Hansen
Keyword(s):  
Gene Expression ◽  
Cdna Library ◽  
Fetal Heart ◽  
Differential Screening ◽  
Subtractive Cdna Library ◽  
Maternal Undernutrition

scholarly journals 347 ETHYLENE-INDUCED GENE EXPRESSION DURING FLOWER PETAL ABSCISSION OF ZONAL GERANIUM

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 480d-480
Author(s):  
David G. Clark ◽  
Kathleen B. Evensen
Keyword(s):  
Gene Expression ◽  
Cdna Library ◽  
Plant Development ◽  
Differential Screening ◽  
Flower Petal ◽  
Pelargonium Xhortorum ◽  
Ethylene Action ◽  
Regulated Gene Expression ◽  
Identification And Characterization

Ethylene-regulated gene expression is being studied in several plant systems, but the exact mechanism of ethylene action during plant development and senescence is poorly understood. When geranium (Pelargonium Xhortorum) flowers are exposed to 1 μ1/L of ethylene gas for 1 hour, petals begin to abscise within 60-90 minutes from the start of treatment, The rapidity of the response implies that it must be very direct. We now demonstrate that ethylene acts at the level of message accumulation. We have constructed a cDNA library from mRNA isolated from ethylene-treated geranium gynoecia. Ethylene-induced clones have been isolated by differential screening of this library with cDNA probes synthesized from ethylene-treated and untreated geranium gynoecia mRNA. Identification and characterization of these clones will be discussed.

scholarly journals Analysis of Expressed Sequence Tag Data and Gene Expression Profiles Involved in Conidial Germination of Fusarium oxysporum

2006 ◽  
Vol 72 (2) ◽  
pp. 1667-1671 ◽  
Author(s):  
Ye Deng ◽  
Haitao Dong ◽  
Qingchao Jin ◽  
Cheng'en Dai ◽  
Yongqi Fang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Synthesis ◽  
Fusarium Oxysporum ◽  
Cdna Library ◽  
Expressed Sequence Tag ◽  
Expression Profiles ◽  
Cdna Array ◽  
Gene Expression Profiles ◽  
Conidial Germination ◽  
Expressed Sequence

ABSTRACT We obtained 3,372 tentative unique transcripts (TUTs) from a cDNA library of Fusarium oxysporum. A cDNA array with 3,158 TUTs was produced to analyze gene expression profiles in conidial germination. It seems that ras and other signaling genes, e.g., ccg, cooperatively initiate conidial germination in Fusarium by increasing protein synthesis.

scholarly journals Differential Screening of a Subtracted cDNA Library: A Method to Search for Genes Preferentially Expressed in Multiple Tissues

BioTechniques ◽  
1997 ◽  
Vol 23 (6) ◽  
pp. 1084-1086 ◽  
Author(s):  
H. Jin ◽  
X. Cheng ◽  
L. Diatchenko ◽  
P.D. Siebert ◽  
C.-C. Huang
Keyword(s):  
Cdna Library ◽  
Differential Screening

scholarly journals Mechanisms for the adverse effects of late gestational increases in maternal cortisol on the heart revealed by transcriptomic analyses of the fetal septum

2014 ◽  
Vol 46 (15) ◽  
pp. 547-559 ◽  
Author(s):  
Elaine M. Richards ◽  
Charles E. Wood ◽  
Maria Belen Rabaglino ◽  
Andrew Antolic ◽  
Maureen Keller-Wood
Keyword(s):  
Gene Expression ◽  
Gene Ontology ◽  
Cell Apoptosis ◽  
Metabolic Pathways ◽  
Fetal Heart ◽  
Pathway Inference ◽  
Apoptosis Pathways ◽  
Altered Metabolism ◽  
Mitochondrial Number ◽  
Muscle Cell Apoptosis

We have previously shown in sheep that 10 days of modest chronic increase in maternal cortisol resulting from maternal infusion of cortisol (1 mg/kg/day) caused fetal heart enlargement and Purkinje cell apoptosis. In subsequent studies we extended the cortisol infusion to term, finding a dramatic incidence of stillbirth in the pregnancies with chronically increased cortisol. To investigate effects of maternal cortisol on the heart, we performed transcriptomic analyses on the septa using ovine microarrays and Webgestalt and Cytoscape programs for pathway inference. Analyses of the transcriptomic effects of maternal cortisol infusion for 10 days (130 day cortisol vs 130 day control), or ∼25 days (140 day cortisol vs 140 day control) and of normal maturation (140 day control vs 130 day control) were performed. Gene ontology terms related to immune function and cytokine actions were significantly overrepresented as genes altered by both cortisol and maturation in the septa. After 10 days of cortisol, growth factor and muscle cell apoptosis pathways were significantly overrepresented, consistent with our previous histologic findings. In the term fetuses (∼25 days of cortisol) nutrient pathways were significantly overrepresented, consistent with altered metabolism and reduced mitochondria. Analysis of mitochondrial number by mitochondrial DNA expression confirmed a significant decrease in mitochondria. The metabolic pathways modeled as altered by cortisol treatment to term were different from those modeled during maturation of the heart to term, and thus changes in gene expression in these metabolic pathways may be indicative of the fetal heart pathophysiologies seen in pregnancies complicated by stillbirth, including gestational diabetes, Cushing's disease and chronic stress.

Single pass cDNA sequencing - a powerful tool to analyse gene expression in preparasitic juveniles of the southern root-knot nematode Meloidogyne incognita

Nematology ◽  
2001 ◽  
Vol 3 (2) ◽  
pp. 129-139 ◽  
Author(s):  
Jaap Bakker ◽  
Fred Gommers ◽  
Geert Smant ◽  
Pierre Abad ◽  
Marie-Noëlle Rosso ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cdna Library ◽  
Meloidogyne Incognita ◽  
Sequence Data ◽  
Homo Sapiens ◽  
Large Fraction ◽  
Nematode Species ◽  
Cdna Libraries ◽  
Parasitic Nematodes ◽  
Root Knot Nematode

AbstractExpressed sequence tags (EST) have been widely used to assist in gene discovery in various organisms (e.g., Arabidopsis thaliana, Caenorhabditis elegans, Mus musculus, and Homo sapiens). In this paper we describe an EST project, which aims to investigate gene expression in Meloidogyne incognita at the onset of parasitism. Approximately 1000 5′-end sequence tags were produced from a cDNA library made of freshly hatched preparasitic second stage juveniles (J2). The EST were identified in the primary transformants of the cDNA library, and assigned to nine different functional groups, including (candidate) parasitism genes. A large fraction of the EST (45%) did not have a putative homologue in public databases. Sixty five percent of the EST that could be clustered into a functional group had putative homologues in other nematode species. EST were found for virtually all parasitism related genes that have been cloned from M. incognita to date. In addition, several novel genes were tagged, including a xylanase and a chitinase gene. The efficiency of EST projects, which produce sequence data for thousands of genes in months time without any difficult pre-selections of mRNA pools, makes random sequencing cDNA libraries a superior method to identify candidates for parasitism related genes in plant-parasitic nematodes. The sequences in this paper are retrievable from Genbank with the accession numbers BE191640 to BE191741, BE217592 to BE217720, BE225324 to BE225598, BE238852 to BE239221, and BE240829 to BE240865.

The addition of 5′-coding information to a 3′-directed cDNA library improves analysis of gene expression

Gene ◽  
1994 ◽  
Vol 146 (2) ◽  
pp. 199-207
Author(s):  
Matoba Ryo ◽  
Okubo Kousaku ◽  
Hori Naohiro ◽  
Fukushima Atsushi ◽  
Matsubara Kenichi
Keyword(s):  
Gene Expression ◽  
Cdna Library

Optimization of RNA Isolation from Brittle Leaf Disease Affected Date Palm Leaves and Construction of a Subtractive cDNA Library

2008 ◽  
Vol 41 (1) ◽  
pp. 63-68 ◽  
Author(s):  
Mohammed Najib Saïdi ◽  
Radhia Gargouri-Bouzid ◽  
Mariem Rayanni ◽  
Noureddine Drira
Keyword(s):  
Cdna Library ◽  
Date Palm ◽  
Rna Isolation ◽  
Subtractive Cdna Library ◽  
Leaf Disease
Sign in / Sign up

Export Citation Format

Share Document