Evaluation of the Antioxidant and Anticancer Activity of Scutellaria barbata, Hedyotis diffusa, and Celastrus hindsii

2020 ◽  
Vol 2 (3) ◽  
pp. 21-25
Author(s):  
Nguyen Viet Quang Nam ◽  
Nguyen Cao Hai Vy ◽  
Nguyen Minh Trung
Keyword(s):  
Anticancer Activity ◽  
Scutellaria Barbata ◽  
Hedyotis Diffusa

scholarly journals Hedyotis diffusa plus Scutellaria barbata Suppress the Growth of Non-Small-Cell Lung Cancer via NLRP3/NF-Κb/MAPK Signaling Pathways

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Ya-Xin Lv ◽  
Hao-Ran Pan ◽  
Xin-Ying Song ◽  
Qing-Qi Chang ◽  
Dan-Dan Zhang
Keyword(s):  
Lung Cancer ◽  
Cell Proliferation ◽  
Mapk Signaling ◽  
Small Cell ◽  
Small Cell Lung ◽  
Scutellaria Barbata ◽  
Hedyotis Diffusa ◽  
Mapk Signaling Pathways

Hedyotis diffusa (HD) plus Scutellaria barbata (SB) have been widely used in antitumor clinical prescribes as one of herb pairs in China. We investigated the effect of aqueous extract from Hedyotis diffusa plus Scutellaria barbata at the equal weight ratio (HDSB11) in inhibiting the growth of murine non-small-cell lung cancer cell (NSCLC) line LLC in vivo and in vitro in this study. Compared with other aqueous extracts, HDSB11 showed the lowest IC50 in inhibiting cell proliferation at 0.43 mg/ml. Besides, HDSB11 effectively suppressed colony formation and induced cell apoptosis. The further assessment of HDSB11 on the murine Lewis-lung-carcinoma-bearing mouse model showed it significantly inhibited tumors’ bioluminescence at the dose of 30 g crude drug/kg. Mechanistically, HDSB11 attenuated the expressions of NLRP3, procaspase-1, caspase-1, PRAP, Bcl-2, and cyclin D1 and downregulated the phosphorylation levels of NF-κB, ERK, JNK, and p38 MAPK. In conclusion, HDSB11 could alleviate cell proliferation and colony formation and induce apoptosis in vitro and tumor growth in vivo, partly via NF-κB and MAPK signaling pathways to suppress NLRP3 expression.

scholarly journals Ethyl Acetate Fraction from Hedyotis diffusa plus Scutellaria barbata Exerts Anti-Inflammatory Effects by Regulating miR-155 Expression and JNK Signaling Pathway

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Yuan Xu ◽  
Xiao-Xia Chen ◽  
Yi-Xin Jiang ◽  
Dan-Dan Zhang
Keyword(s):  
Ethyl Acetate ◽  
Signaling Pathway ◽  
Ethyl Acetate Fraction ◽  
Equal Weight ◽  
Dependent Manner ◽  
Jnk Signaling ◽  
Scutellaria Barbata ◽  
Hedyotis Diffusa ◽  
Jnk Signaling Pathway ◽  
Anti Inflammatory

Hedyotis diffusa Willd and Scutellaria barbata D. Don (HDSB) were the core couplet in medicines that were commonly used for the purpose of anti-inflammation and anticancer treatments in China. However, biological properties of this couplet have not been fully elucidated. In this study, we screened fractions of HDSB for their anti-inflammatory activities and explored pertinent molecular mechanisms on murine macrophage RAW264.7 cell model. Ethyl acetate fraction from the aqueous extract of the couplet at equal weight ratio (EA11) showed the strongest inhibition of the nitrite accumulation in supernatant of RAW264.7 cells stimulated with lipopolysaccharide (LPS)/interferon-γ (IFN-γ). In addition, EA11 inhibited iNOS and IL-1β expression in a concentration-dependent manner while promoting the expression of HO-1 and PPAR-γ. Anti-inflammatory capability is most likely facilitated by its inhibitory effect on JNK signaling pathway and miR-155 expression. This study suggests that EA11 may be represented as a potential anti-inflammatory therapeutic candidate.

scholarly journals Hedyotis diffusaplusScutellaria barbataInduce Bladder Cancer Cell Apoptosis by Inhibiting Akt Signaling Pathway through Downregulating miR-155 Expression

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Li-Tao Pan ◽  
Yip Sheung ◽  
Wen-Peng Guo ◽  
Zhi-Bin Rong ◽  
Zhi-Ming Cai
Keyword(s):  
Bladder Cancer ◽  
Cancer Cell ◽  
Cell Apoptosis ◽  
Bladder Cancer Cell ◽  
Dependent Manner ◽  
Cancer Cell Growth ◽  
Scutellaria Barbata ◽  
Akt Activation ◽  
The Core ◽  
Hedyotis Diffusa

Traditional Chinese medicine is increasingly used to treat cancer. Our clinical experiences identifyHedyotis diffusaplusScutellaria barbataas the most common herb-pair (couplet medicinal) used for the core treatment of bladder cancer. This study aims to investigate the antitumor effect of the herb-pair in bladder cancer cells. The results show thatHedyotis diffusaplusScutellaria barbatainhibited bladder cancer cell growth and clone formation in a dose-dependent and time-dependent manner. It also induced cell apoptosis through decreasing Akt activation and reducing the expression of antiapoptotic proteins Bcl-2 and Mcl-1. Further experiments showed that miR-155 was reduced by the herb-pair and miRNA-155 inhibitor induced cell apoptosis and suppressed Akt activation. Overexpression of miR-155 reversed herb-pair induced cell apoptosis through activating Akt pathway in both bladder cancer cell lines. The findings reveal thatHedyotis diffusaplusScutellaria barbatareduce Akt activation through reducing miR-155 expression, resulting in cell apoptosis. It demonstrated the potential mechanism ofHedyotis diffusaplusScutellaria barbatafor the core treatment of bladder cancer.

scholarly journals Ethyl Acetate Fraction from Hedyotis diffusa plus Scutellaria barbata Suppresses Migration of Bone-Metastatic Breast Cancer Cells via OPN-FAK/ERK/NF-κB Axis

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Ting Fang ◽  
Ying-Xuan Yan ◽  
Yue Yang ◽  
Ya-Xin Lv ◽  
Qing-Qi Chang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Migration ◽  
Bone Metastasis ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Equal Weight ◽  
Dependent Manner ◽  
Scutellaria Barbata ◽  
Hedyotis Diffusa ◽  
Mcf 7

Hedyotis diffusa plus Scutellaria barbata is a couplet of medicinal that has been commonly used to treat inflammation-related diseases and various types of tumors. However, the effect of this couplet on tumor cell migration has not been elucidated. With the aid of MCF-7-BOM, a bone-metastatic subline of ER + breast cancer MCF-7, we showed that ethyl acetate fraction extracted at an equal weight ratio of Hedyotis diffusa plus Scutellaria barbata (EA11) inhibited cell migration of MCF-7-BOM in a concentration-dependent manner. To define the underlying molecular mechanism, we revealed that EA11 reduced the expression of osteopontin (OPN) and interfered with the FAK/ERK/NF-κB signaling pathways, which are both critical for breast cancer bone metastasis. This study strongly suggested EA11 may represent a potential therapeutic agent against bone metastasis of breast cancer.

Simultaneous Determination of 15 Flavonoids in Scutellaria barbata–Hedyotis diffusa Herb Pair by HPLC Q-TOF MS

2019 ◽  
Vol 102 (1) ◽  
pp. 75-80 ◽  
Author(s):  
Wei Yang ◽  
Shiyong He ◽  
Nan Xiao ◽  
Yanling Qiao ◽  
Hua Sui ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Stability Study ◽  
Scutellaria Barbata ◽  
Inflammatory Conditions ◽  
Hedyotis Diffusa ◽  
Relative Standard ◽  
Peak Areas ◽  
The Stability ◽  
Tof Ms

Abstract Background: The Scutellaria barbata – Hedyotis diffusa herb pair has appeared in classical prescriptions and has usually been used for the treatment of cancer and inflammatory conditions. Objective: For quality control of this herb pair, a rapid and reliable HPLC-quadrupole-time-of-flight(Q-TOF)-MS method was used for the simultaneous determination of alpinetin, apigenin-7-O-β-D-glucopyranoside, scutellarein, apigenin, quercetin-3-O-β-D-glucopyranoside, wogonoside, quercetin, amentoflavone, wogonin, chrysin, luteolin, rutin, naringenin, baicalein, and baicalin in the S. barbata–H. diffusa herb pair. Methods: LC-MS analysis was performed on a Shimadzu HPLC 20ADXR LC system in-line with an AB-SCIEX 5600 Triple TOF mass spectrometer. Results: The calibration curves showed good linearity in a relatively wide concentration range (r >0.996). The LOD and LOQ were less than 20 and 68 ng/mL, respectively. The relative standard deviation (RSD) values of intraday and inter-day precision were <3.31% for all the analytes. The recoveries of the method were in the range of 94.6–106.0%, with RSD <4.72%. The RSD values of peak areas in the stability study were not more than 4.28% for all analytes within 2 days. These results revealed that the method exhibited good sensitivity, precision, accuracy, and stability. Conclusions: This method could be applied to the quantitative determination of 15 flavonoids in the S. barbata – H. diffusa herb pair. Highlights: (1) The established method of HPLC-Q-TOF-MS is simple, fast, sensitive, and reliable. (2) 15 Flavonoids in the S. barbata–H. diffusa herb pair were simultaneous determined.

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