scholarly journals Less light, more growth? Effects of the abscemce of light on roots of in vitro of Catasetum fimbriatum (Orchidaceae)

2019 ◽  
Vol 1 (2) ◽  
pp. 14-16
Author(s):  
Regina A. Amadeu
Keyword(s):  
Growth Effects ◽  
Catasetum Fimbriatum

Progastrin1–80stimulates growth of intestinal epithelial cells in vitro via high-affinity binding sites

2003 ◽  
Vol 284 (2) ◽  
pp. G328-G339 ◽  
Author(s):  
P. Singh ◽  
X. Lu ◽  
S. Cobb ◽  
B. T. Miller ◽  
N. Tarasova ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Binding Sites ◽  
Intestinal Epithelial Cells ◽  
Full Length ◽  
Intestinal Epithelial ◽  
Growth Effects ◽  
High Affinity ◽  
Significant Difference

Proliferation and carcinogenesis of the large intestinal epithelial cells (IEC) cells is significantly increased in transgenic mice that overexpress the precursor progastrin (PG) peptide. It is not known if the in vivo growth effects of PG on IEC cells are mediated directly or indirectly. Full-length recombinant human PG (rhPG1–80) was generated to examine possible direct effects of PG on IEC cells. Surprisingly, rhPG (0.1–1.0 nM) was more effective than the completely processed gastrin 17 (G17) peptide as a growth factor. Even though IEC cells did not express CCK1and CCK2receptors (-R), fluorescently labeled G17 and Gly-extended G17 (G-Gly) were specifically bound to the cells, suggesting the presence of binding proteins other than CCK1-R and CCK2-R on IEC cells. High-affinity ( Kd= 0.5–1.0 nM) binding sites for125I-rhPG were discovered on IEC cells that demonstrated relative binding affinity for gastrin-like peptides in the order PG ≥ COOH-terminally extended G17 ≥ G-Gly > G17 > *CCK-8 (* significant difference; P< 0.05). In conclusion, our studies demonstrate for the first time direct growth effects of the full-length precursor peptide on IEC cells in vitro that are apparently mediated by the high-affinity PG binding sites that were discovered on these cells.

Promising anti-growth effects of palladium(II) saccharinate complex of terpyridine by inducing apoptosis on transformed fibroblasts in vitro

2013 ◽  
Vol 21 (15) ◽  
pp. 4698-4705 ◽  
Author(s):  
Melis Debreli Coskun ◽  
Ferda Ari ◽  
Arzu Yilmaztepe Oral ◽  
Mehmet Sarimahmut ◽  
Hatice M. Kutlu ◽  
...  
Keyword(s):  
Growth Effects ◽  
Transformed Fibroblasts

In Vitro Growth Effects of Colony-Stimulating Factors in Ovarian Cancer

1994 ◽  
Vol 52 (3) ◽  
pp. 347-352 ◽  
Author(s):  
Joseph P. Connor ◽  
Robert C. Squatrito ◽  
Kristina L. Terrell ◽  
Britt J. Antisdel ◽  
Richard E. Buller
Keyword(s):  
Ovarian Cancer ◽  
In Vitro Growth ◽  
Colony Stimulating Factors ◽  
Growth Effects

scholarly journals pp60c-Src Kinase Mediates Growth Effects of the Full-Length Precursor Progastrin1–80 Peptide on Rat Intestinal Epithelial Cells, in Vitro

Endocrinology ◽  
2003 ◽  
Vol 144 (1) ◽  
pp. 201-211 ◽  
Author(s):  
D. Brown ◽  
U. Yallampalli ◽  
A. Owlia ◽  
P. Singh
Keyword(s):  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Calf Serum ◽  
Src Kinase ◽  
Inhibitory Effect ◽  
Full Length ◽  
Intestinal Epithelial ◽  
Growth Effects ◽  
In Cells

Abstract Growth factor effects of precursor forms of gastrins have become evident in recent years. However, intracellular pathways that mediate growth effects of the precursor molecules are not known. In previous studies, we reported an increase in Tyr phosphorylation of pp60c-Src in intestinal epithelial cells (IEC) in response to the fully processed form of gastrin [gastrin1–17 (G17)]. We have now examined whether c-Src kinase is similarly phosphorylated and activated in response to the full-length precursor molecule, progastrin (PG)1–80, (recombinant human PG) in IEC cells. We found a significant increase in pp60c-Src kinase activity in response to both G17 and PG (0.1–1.0 nm), suggesting that growth effects of both the precursor and fully processed gastrin molecules may be mediated via similar pathways. On the other hand, pp62c-Yes was not phosphorylated or activated in response to either G17 or PG. To examine whether c-Src kinase mediates proliferative effects of PG, IEC cells were microinjected with anti-Src-IgG and 3H-thymidine (3H-Tdr) uptake of the cells measured. Control cells received nonimmune IgG. The 3H-Tdr uptake of cells stimulated with 1.0 nm PG was significantly reduced in cells microinjected with anti-c-Src-IgG; control IgG had no effect. In cells stimulated with 1.0% fetal calf serum, microinjection with c-Src-IgG had no effect on 3H-Tdr uptake. The specificity of the effect was further confirmed by blocking the inhibitory effect of anti-c-Src-IgG with antigenic Src peptide. These results suggest that activation of c-Src kinase likely represents a critical step in mediating proliferative effects of both the precursor and fully processed forms of gastrins on IEC.

scholarly journals Genistein inhibits estradiol- and environmental endocrine disruptor-induced growth effects on neuroblastoma cells in vitro

2013 ◽  
Vol 5 (5) ◽  
pp. 1583-1586 ◽  
Author(s):  
JICUI ZHENG ◽  
HUI LI ◽  
HAITAO ZHU ◽  
XIANMIN XIAO ◽  
YANGYANG MA
Keyword(s):  
Endocrine Disruptor ◽  
Neuroblastoma Cells ◽  
Growth Effects

Poly(dl-lactic acid)/triblock PCL–PDMS–PCL copolymers: synthesis, characterization and demonstration of their cell growth effects in vitro

Polymer ◽  
2001 ◽  
Vol 42 (9) ◽  
pp. 4109-4116 ◽  
Author(s):  
N Kayaman-Apohan ◽  
O Karal-Yılmaz ◽  
K Baysal ◽  
B.M Baysal
Keyword(s):  
Lactic Acid ◽  
Cell Growth ◽  
Growth Effects

scholarly journals Paradoxical Growth of Candida dubliniensis Does Not Preclude In Vivo Response to Echinocandin Therapy

2009 ◽  
Vol 53 (12) ◽  
pp. 5297-5299 ◽  
Author(s):  
Marçal Mariné ◽  
F. Javier Pastor ◽  
Ismail H. Sahand ◽  
José Pontón ◽  
Guillermo Quindós ◽  
...  
Keyword(s):  
Systemic Infection ◽  
Candida Dubliniensis ◽  
Clinical Isolates ◽  
Murine Models ◽  
Growth Effects ◽  
Abnormal Growth ◽  
Paradoxical Growth

ABSTRACT Candida dubliniensis commonly shows paradoxical or trailing growth effects in vitro in the presence of echinocandins. We tested the in vitro activities of anidulafungin, caspofungin, and micafungin against clinical isolates of C. dubliniensis and evaluated the efficacy of these drugs in two murine models of systemic infection. The three echinocandins were similarly effective in the treatment of experimental disseminated infections with C. dubliniensis strains showing or not showing abnormal growth in vitro.

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