scholarly journals Short tandem repeat typing technologies used in paternity testing: a case study

2020 ◽  
pp. 04-09
Author(s):  
Amany Abdel Hakim Mousa ◽  
Dena Mohamed Naguib Abdel Moawed ◽  
Adel Mohamed Abdallah Elberry
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Nuclear Dna ◽  
Pcr Amplification ◽  
Str Typing ◽  
Biological Father ◽  
Str Analysis ◽  
Dna Profile ◽  
Federal Bureau Of Investigation ◽  
Short Tandem

Background: In forensic field, for establishing the paternity of disputed offspring, STR typing (autosomal, and Y typing) are commonly used to dissolve the cases. Short tandem repeat (STR) technology is used to evaluate specific regions (loci) within nuclear DNA. Variability in STR regions can be used to distinguish one DNA profile from another. The Federal Bureau of Investigation (FBI) uses a standard set of 13 specific STR regions for CODIS. Aim of the study: The present work was done to find out the biological father of child in a case where mother was killed by her husband for the doubt of her pregnancy. Methods: After taking blood samples from the murdered mother, the baby and the killer husband, Autosomal STRs Using AmpFlSTR Identifiler Plus PCR Amplification Kit and Y-STR analysis using AmpFlSTR Yfiler PCR Amplification Kit were done to solve the disputed paternity case. Results: After the analysis of DNA STRs profiles of autosomal and Y-chromosome markers, the dead fetus was proved to be the son of the killer father. Conclusions: Autosomal STR multiplex analysis combined with Y-STR analysis help to reach a conclusion in disputed paternity cases with greater confidence. Keywords: Disputed; Paternity; Multiplex; Short tandem reprats; Y-STR; Analysis

DNA analysis : Current status in court

2004 ◽  
Vol 1 (3) ◽  
Author(s):  
W. J. Wall
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Dna Analysis ◽  
Current Status ◽  
Str Analysis ◽  
Dna Profile ◽  
Single Locus Probes ◽  
Or Groups ◽  
Starting Point ◽  
Short Tandem

AbstractSince the introduction of DNA profiling the methods have change considerably and so have the way that results are expressed. There have been many different techniques used to produce a DNA profile. The starting point was multilocus probes, followed by single locus probes, neither of which are now widely used, and then Short tandem Repeat (STR) analysis. Whatever the next step in DNA analysis is, the methods of interpretation will remain essentially the same. We rely on the concept and reality of the benign state for this to be used for the good of society, not the oppression of individuals or groups.

Evaluation genotypes of cancer cell lines HCC1954 and SiHa by short tandem repeat (STR) analysis and DNA sequencing

2018 ◽  
Vol 45 (6) ◽  
pp. 2689-2695 ◽  
Author(s):  
Jiewen Fu ◽  
Jingliang Cheng ◽  
Xiaoyan Liu ◽  
Jun Li ◽  
Chunli Wei ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Cell Lines ◽  
Cancer Cell ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Cancer Cell Lines ◽  
Str Analysis ◽  
Short Tandem

Variant alleles observed in short tandem repeat (STR) analysis of random Indian population

2019 ◽  
Vol 19 (1) ◽  
pp. 94
Author(s):  
Monika Chakravarty ◽  
Nidhi Singh ◽  
Damini Varshney ◽  
Dhruw Sharma ◽  
Naresh Kumar ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Indian Population ◽  
Str Analysis ◽  
Variant Alleles ◽  
Short Tandem

Automated short tandem repeat (STR) analysis in forensic casework — a strategy for the future

1995 ◽  
Vol 16 (1) ◽  
pp. 1543-1552 ◽  
Author(s):  
Peter Gill ◽  
Colin P. Kimpton ◽  
Andrew Urquhart ◽  
Nicola Oldroyd ◽  
Emma S. Millican ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Str Analysis ◽  
Forensic Casework ◽  
The Future ◽  
Short Tandem

scholarly journals Research Resource: STR DNA Profile and Gene Expression Comparisons of Human BG-1 Cells and a BG-1/MCF-7 Clonal Variant

2014 ◽  
Vol 28 (12) ◽  
pp. 2072-2081 ◽  
Author(s):  
Yin Li ◽  
Yukitomo Arao ◽  
Julie M. Hall ◽  
Sandra Burkett ◽  
Liwen Liu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ovarian Cancer ◽  
Cell Line ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Expression Patterns ◽  
Genotypic Differences ◽  
Dna Profile ◽  
Short Tandem ◽  
Mcf 7

Human ovarian cancer BG-1 cells are a valuable in vitro model that has enabled several laboratories to study the estrogenic responses of ovarian cancers. We recently discovered that there are two different BG-1 cell lines being used for experiments, denoted here as BG-1 FR and BG-1 NIEHS, which exhibit striking morphological differences. The objective of this study was to methodically analyze these two BG-1 variants and compare their characteristics. Short tandem repeat analysis revealed that the DNA profile of BG-1 FR cells was unique, yet the Short tandem repeat pattern of BG-1 NIEHS was identical with that of MCF-7 cells. From a cytogenetic analysis, it became apparent that the BG-1 FR line had the same profile as previously reported, whereas the BG-1 NIEHS and MCF-7 cells share a similar genetic display. A significant number of unique chromosomal translocations were observed between the BG-1 NIEHS and MCF-7 cells, suggesting that acquired genotypic differences resulted in the formation of two lines from a common origin. Although all cell types demonstrated a similar estrogen responsiveness in reporter gene assays, a microarray analysis revealed distinct estrogen-responsive gene expression patterns with surprisingly moderate to low overlap. We conclude that BG-1 FR is the original ovarian cancer cell line, whereas the BG-1 NIEHS is a variant from the MCF-7 cells. These findings provide much needed clarification of the identities and characteristics of key cell line models that are widely used to study estrogen action in female reproductive cancers.

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