scholarly journals EFFECT OF ULTRASOUND ON S. EPIDERMIDIS MUSEUM CULTURE

2020 ◽  
Vol 73 (4) ◽  
pp. 786-788
Author(s):  
Yuliia V. Sidash ◽  
Olha N. Boychenko ◽  
Ivan Yu. Popovych ◽  
Andrii V. Zaytsev
Keyword(s):  
Scientific Literature ◽  
Bacterial Culture ◽  
Antimicrobial Effect ◽  
Ultrasonic Waves ◽  
Empirical Methods ◽  
Viable Cells ◽  
Comparison Measurement ◽  
Glass Tubes ◽  
Measurement Experiment ◽  
Museum Culture

The aim of the present research project was to study the effect of ultrasonic waves on the S. epidermidis ATCC 14990 museum culture. Materials and methods: A bibliosemantic method – for analyzing previous research based on scientific literature, electronic resources, empirical methods of scientific research (observation, comparison, measurement, experiment) – to study the effect of ultrasound on the strain of S. epidermidis ATCC 14990. Results: A study showed that when exposed to ultrasound for four and a half minutes on a culture of microorganisms S. epidermidis in glass tubes, the number of viable cells in the suspension decreases. Conclusions: The influence of ultrasound on the culture of S. epidermidis, studied in the work, showed that the selected and used parameters of ultrasound treatment cause destructive changes in microorganisms. These changes are not fatal for the entire bacterial culture used in the study, but they have a reliable antimicrobial effect.

scholarly journals Role of single and mixed culture of Pseudomonas aeruginosa and Serratia ficaria in utilization of petroleum wastes of Dora- refineries.

2007 ◽  
Vol 4 (4) ◽  
pp. 542-548
Author(s):  
Baghdad Science Journal
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Mixed Culture ◽  
Bacterial Culture ◽  
Bacterial Cultures ◽  
Viable Cells ◽  
Nacl Concentration ◽  
Agitated Culture ◽  
Growth Of Bacteria ◽  
Better Than

The ability of single and mixed bacterial culture to utilize Dora-refineries petroleum wastes was compared. Pseudomonas aeruginosa and Serratia ficaria mixed culture consumed the wastes better than the single bacterial cultures. The highest log. number of viable cells in mixed culture was 6.842 , while in single bacterial cultures it was 6.683 and 5.631, respectively. after 3 days in API medium containing the refinery wastes. The effect of some environmental conditions on the degradation of petroleum wastes was studied included aeration , NaCl concentration , pH and temperature. The growth of bacteria in the agitated culture was higher than stagnant culture the log. of cell no. was 6.021 in the first culture. The highest log. of cell no. stagnant culture was 5.771. Pseudomonas aeruginosa AA22 and Serratia ficaria AA39 were able to grow in medium containing 5 , 7 % NaCl , they favorite pH 7. The mixed culture of the two bacteria grew well of 45 oC.

scholarly journals PROFESSIONAL READINESS OF THE TEACHERS TO CREATE ELECTRONIC EDUCATIONAL CONTENT (BY THE EXAMPLE OF PROFESSIONAL DEVELOPMENT PROGRAMS)

2020 ◽  
pp. 71-80
Author(s):  
O.B. Akimova ◽  
◽  
V.A. Zavyalova
Keyword(s):  
Scientific Literature ◽  
Learning Technologies ◽  
Educational Technologies ◽  
Development Programs ◽  
Empirical Methods ◽  
Educational Content ◽  
Professional Programs ◽  
Effective Implementation ◽  
E Learning ◽  
Distance Learning Technologies

The possibility of implementing additional professional programs using e-learning and distance learning technologies is discussed. The condition for the effectiveness of this activity is the professional readiness of the teacher to create electronic educational content. The scientific literature on the problems of teachers' readiness has been analyzed. Components have been identified and criteria for professional readiness have been defined. The purpose of the work was to identify the readiness of teachers to create electronic educational content. On the basis of theoretical and empirical methods the conclusions were made that a condition of qualitative organization of the teacher's own activity on creation of electronic educational content is a set of all components of professional readiness. Thus, the task of effective implementation of additional professional programs with the use of e-learning and distance educational technologies is solved.

Formation of ‘non-culturable’ cells of Mycobacterium smegmatis in stationary phase in response to growth under suboptimal conditions and their Rpf-mediated resuscitation

Microbiology ◽  
2004 ◽  
Vol 150 (6) ◽  
pp. 1687-1697 ◽  
Author(s):  
Margarita Shleeva ◽  
Galina V. Mukamolova ◽  
Michael Young ◽  
Huw D. Williams ◽  
Arseny S. Kaprelyants
Keyword(s):  
Stationary Phase ◽  
Mycobacterium Smegmatis ◽  
Bacterial Culture ◽  
Simultaneous Action ◽  
Wild Type ◽  
Low Oxygen ◽  
Viable Cells ◽  
Transient Loss ◽  
Isogenic Strains ◽  
Endogenous Synthesis

Conditions were investigated that promote the formation of ‘non-culturable’ (NC) cells of Mycobacterium (Myc.) smegmatis in stationary phase. After cultivation in a rich medium, or under conditions that may be considered optimal for bacterial growth, or starvation for carbon, nitrogen or phosphorus, bacteria failed to enter a NC state. However, when grown under suboptimal conditions, resulting in a reduced growth rate or maximal cell concentration (e.g. in modified Hartman's–de Bont medium), bacteria adopted a stable NC state after 3–4 days incubation in stationary phase. Such conditions are not specific as purF and devR mutants of Myc. smegmatis also showed (transient) loss of culturability following growth to stationary phase in an optimized medium, but under oxygen-limited conditions. The behaviour of the same mutants in oxygen-sufficient but nutrient-inappropriate medium (modified Hartman's–de Bont medium) was similar to that of the wild-type (adoption of a stable NC state). It is hypothesized that adoption of a NC state may represent an adaptive response of the bacteria, grown under conditions when their metabolism is significantly compromised due to the simultaneous action of several factors, such as usage of inappropriate nutrients or low oxygen availability or impairment of a particular metabolic pathway. NC cells of wild-type Myc. smegmatis resume growth when transferred to a suitable resuscitation medium. Significantly, resuscitation was observed when either recombinant Rpf protein or supernatant derived from a growing bacterial culture was incorporated into the resuscitation medium. Moreover, co-culture with Micrococcus (Mcc.) luteus cells (producing and secreting Rpf) also permitted resuscitation. Isogenic strains of Myc. smegmatis harbouring plasmids containing the Mcc. luteus rpf gene also adopt a similar NC state after growth to stationary phase in modified Hartman's–de Bont medium. However, in contrast to the behaviour noted above, these strains resuscitated spontaneously when transferred to the resuscitation medium, presumably because they are able to resume endogenous synthesis of Mcc. luteus Rpf. Resuscitation was not observed in the control strain harbouring a plasmid lacking Mcc. luteus rpf. In contrast to wild-type, the NC cells of purF and devR mutants obtained under oxygen-limited conditions resuscitate spontaneously, presumably because the heterogeneous population contains some residual viable cells that continue to make Rpf-like proteins.

Antimicrobial Effect of Chlorine on Listeria monocytogenes

1987 ◽  
Vol 50 (12) ◽  
pp. 999-1003 ◽  
Author(s):  
R. E. BRACKETT
Keyword(s):  
Phosphate Buffer ◽  
Sodium Thiosulfate ◽  
Potassium Phosphate ◽  
Antimicrobial Effect ◽  
Residual Chlorine ◽  
Brussels Sprouts ◽  
Viable Cells ◽  
Colony Forming Units ◽  
Free Residual Chlorine ◽  
Effect Of Chlorine

The antimicrobial effect of reagent-grade sodium hypochlorite (SH) and household bleach (HB) on 2 strains of Listeria monoctiytogenes (Scott A and LCDC 81-861, both serotype 4a) was determined. After 24 h of growth in tryptic soy broth, cells were centrifuged, and pellets resuspended in potassium phosphate buffer (pH 7.0). Three-milliliter portions of the cell suspensions were then added to 27 ml of phosphate buffer containing about 0, 5, 10, 50, 100, or 200 ppm free residual chlorine. Cells were exposed to the chlorine for 20, 60, 120 and 300 s, at which time the chlorine was neutralized with 0.01 M sodium thiosulfate. Populations of surviving cells were determined by plating samples of the neutralized solution on tryptic soy agar and incubating the plates for 48 h at 30°C before counting. Chlorine concentrations less than about 50 ppm showed no antimicrobial effect but exposure to 50 ppm or greater chlorine resulted in no viable cells being recovered. Results for both SH and HB were similar. Dipping Brussels sprouts containing about 6 log10 colony forming units (CFU) L. monocytogenes/g into a 200-ppm chlorine solution for 10 s reduced viable cells recovered on McBrides agar by about 2 log10 CFU/g. Dipping Brussels sprouts in water alone reduced populations by about 1 log10 CFU/g.

scholarly journals Scientific potential of Russian women

2021 ◽  
Vol 5 (S3) ◽  
pp. 1172-1184
Author(s):  
Aleksandr M. Egorychev ◽  
Anna G. Akhtyan ◽  
Anatoly K. Bykov ◽  
Elina N. Rychikhina ◽  
Svetlana V. Lvova ◽  
...  
Keyword(s):  
Scientific Literature ◽  
Educational Institution ◽  
Historical Analysis ◽  
Scientific Activity ◽  
Empirical Methods ◽  
Russian State ◽  
State And Society ◽  
Scientific Potential ◽  
Young Students ◽  
Gender Specific

Based on historical analysis of the scientific literature, the paper demonstrates the role, importance, and necessity of developing the female scientific potential in the Russian state and society. The sphere of young students' scientific interests has been identified and substantiated with gender consideration. Similarly, possibilities of optimizing the potential of young students have been found with gender-specific aspects borne in mind. In the research, the authors used theoretical and empirical methods of collecting information about the scientific potential of Russian women. An entire range of problems (causes) has been found which prevent the potential of Russian women from being completely fulfilled in science. Principal conditions and mechanisms have been examined which help optimize the development of the scientific potential of young higher educational institution students with gender consideration. The obtained conclusions highlight the necessity of optimizing the female potential in the country's scientific activity in the most diverse focus areas.

scholarly journals Youth unemployment: research of problems and needs

2021 ◽  
Vol 24 (1) ◽  
pp. 35-44
Author(s):  
Lyudmila Melnik ◽  
Olena Danilyuk ◽  
Natalia Gevchuk
Keyword(s):  
Young People ◽  
Job Search ◽  
Scientific Literature ◽  
Youth Unemployment ◽  
Market Development ◽  
Empirical Methods ◽  
Job Offers ◽  
Article Analysis ◽  
Employment Center ◽  
Unemployed Youth

The problems and needs of unemployed youth on the example of unemployed young people in Kamyanets-Podilsky district of Khmelnytsky region and those registered in Kamyanets-Podilsky district branch of Khmelnytsky regional employment center are analyzed in the article. Studies of youth unemployment, its causes, types and consequences are one of the key socio-economic studies, which not only do not lose their relevance, but also, in modern conditions, acquire new dimensions. The application of general theoretical methods of analysis and generalization of the scientific literature on the problem in the process of research made it possible to conduct a theoretical analysis of the definitions of "unemployment", "unemployed" and to determine the theoretical meaning of "unemployed youth". The use of a set of empirical methods, such as interviews, surveys (questionnaires), statistical analysis, made it possible to determine the number of unemployed persons by age, the level of education of unemployed youth, duration of the period of staying unemployed being registered at the employment center, expected results from applying to the employment center and being registered, the most common types of professions in which young people have previous experience, the level of expected wages of unemployed citizens at the new place of work, methods of independent job search. Summarizing the results of the study, conclusions on the causes of youth unemployment, in particular the causes of unemployment of young women, the reasons for rejection of job offers, problems and needs of young people who are looking for job; the role and importance of the employment center in future employment are made. The prospects for further research in this area are also outlined in the article: analysis and development of ways to increase employment among unemployed youth; identification of the most relevant and popular professions in the labor market, development and implementation of innovative forms of employment centers specialists’ work with unemployed youth.

scholarly journals Preparation and Functional Properties of Synbiotic Yogurt Fermented with Lactobacillus brevis PML1 Derived from a Fermented Cereal-Dairy Product

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Fereshteh Falah ◽  
Alireza Vasiee ◽  
Farideh Tabatabaee Yazdi ◽  
Behrooz Alizadeh Behbahani
Keyword(s):  
Functional Foods ◽  
Dairy Product ◽  
Antimicrobial Effect ◽  
Probiotic Strain ◽  
Lactobacillus Brevis ◽  
Fermented Food ◽  
Antimicrobial Compounds ◽  
Normal Range ◽  
Physiochemical Properties ◽  
Viable Cells

Nowadays, production of functional foods has become very essential. Inulin is one of the most functional hydrocolloid compounds used in such products. In the present study, the production of a synbiotic yogurt containing 1, 2.5, and 5% ( w / v ) inulin has been investigated. The yogurt was fermented with Lactobacillus brevis PML1 derived from Tarkhineh, an Iranian cereal-dairy fermented food. Furthermore, the physicochemical properties, antioxidant activity, sensory attributes, and microbial viability properties were investigated on the 0th, 7th, and 14th days of storage after fermentation. The viable cells of L. brevis PML1 reached 108 CFU/g, and the product resisted to simulated digestive juices. Moreover, the synbiotic yogurt impressively increased the production of antimicrobial compounds and had the most profound antimicrobial effect on S. typhimurium. The physiochemical properties were in the normal range, and the fat content of the synbiotic yogurt was reduced remarkably. The antioxidant capacity of the fermented yogurt was significantly increased ( p < 0.05 ), which was equal to those of DPPH ( 69.18 ± 1.00 % ) and BHA ( 89.16 ± 2.00 % ). The viability of L. brevis PML1 was increased during storage. Sensory analysis showed that there were significant differences in terms of the impressive parameters between the samples and the control ( p < 0.05 ). Addition of 2.5% inulin not only improved the physical properties but also retained the viability of the probiotic after 14 days of storage, in addition to the viability of L. brevis with a viability count above 6 log CFU/g in the yogurt. Therefore, a novel synbiotic product containing L. brevis PML1, which can exert the desired properties, can be used as a suitable carrier for the delivery of the probiotic strain, exerting its beneficial health effects.

scholarly journals TO THE ISSUE OF THE CONCEPT OF THE STATE TASK AS A FINANCIAL INSTRUMENT IN THE SPHERE OF EDUCATION AND ITS LEGAL REGULATION

2021 ◽  
Vol 17 (1(63)) ◽  
pp. 201-211
Author(s):  
Надежда Николаевна АНДРЕЯНОВА ◽  
Лариса Владимировна НАУМОВА
Keyword(s):  
Scientific Literature ◽  
Legal Regulation ◽  
The State ◽  
Municipal Services ◽  
Empirical Methods ◽  
Financial Instrument ◽  
Legal Norms ◽  
The Subject ◽  
The Russian Federation ◽  
Theoretical Foundations

The financial provision of state and municipal services is being actively improved at the legislative level, and therefore is the subject of interest of jurisprudence and economics, which determines the relevance of the chosen topic. Purpose: to study scientific literature on the theoretical foundations of the concept of «finance», and regulatory legal acts of the Russian Federation related to the concepts of «financing» and «state task» for disclosing the concepts of financial provision of state and municipal services in the sphere of education. Methods: the authors use the empirical methods of comparison, description, interpretation, legal-dogmatic and as well as the method of interpretation of legal norms. Results: the authors’ interpretation of the concept of «state task» is given, it is concluded that the state task is a financial instrument in the budgetary sphere.

scholarly journals ENSURING PHYSICAL AND MENTAL HEALTH OF ATHLETES BY THE LABOR LAW

2021 ◽  
Vol 74 (11) ◽  
pp. 3025-3030
Author(s):  
Olena V. Moskalenko ◽  
Nataliia O. Melnychuk ◽  
Denys O. Novikov
Keyword(s):  
Mental Health ◽  
Direct Relationship ◽  
Scientific Literature ◽  
Labor Law ◽  
Labor Rights ◽  
Empirical Methods ◽  
Working Capacity ◽  
Physical And Mental Health ◽  
Regional Organizations ◽  
Failure To Protect

The aim: To identify the problems of exclusion of athletes from the sphere of labor law; characterize the main precarious practices in sports that are contrary to labor law, that lead to injury of athletes. Materials and methods: The research materials cover the analysis of scientific literature, legal acts and jurisprudence; comparison; synthesis; generalization. Empirical methods related to the statistics processing of reports of international and regional organizations (ILO, EU Athletes, European Commission, FICA, FIFPro, United Nations) serve to combine and graphically display information in the field of sports. Conclusions: Precarious practices in sports are widespread; cover all athletes (from elite to non-professional); constitute a threat to the health and working capacity of athletes. A direct relationship has been revealed between the regulation of the labor of athletes and their physical and mental health. Failure to protect labor rights increases the risks of disability for athletes. The international community and national states should reduce the inclusion of the so-called “sports exemption” and create legal mechanisms for transferring the labor of athletes to the scope of labor law.

Sign in / Sign up

Export Citation Format

Share Document