scholarly journals REMODELING OF THE DUCT SYSTEM OF THE RAT SUBMANDIBULAR SALIVARY GLANDS IN CHRONIC ETHANOL INTOXICATION

2020 ◽  
Vol 73 (1) ◽  
pp. 128-133 ◽  
Author(s):  
Kostyantyn V. Shevchenko ◽  
Galina A. Yeroshenko ◽  
Оlena V. Vilkhova ◽  
Denis R. Kramarenko ◽  
Оlena S. Yakushko ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Conventional Technique ◽  
Chronic Ethanol ◽  
Lumen Diameter ◽  
Control Group ◽  
Duct System ◽  
Ethanol Intoxication ◽  
Submandibular Glands ◽  
Chronic Ethanol Intoxication ◽  
Granular Ducts

The aim: To determine the dynamics of changes in metric parameters of the duct system of rat submandibular glands in normal conditions and chronic ethanol intoxication. Materials and methods: 50 albino outbred rats were involved in the experiment. 10 animals were assigned in the control group, 40 animals – in the experimental group. Animals were sacrificed on 5, 9, 12 and 30 days by overdose of thiopental anesthesia. Pieces of the submandibular glands were embedded into epon-812 according to the conventional technique. Results: On day 5 of the experiment the lumen diameter of intercalated duct reduced by 9,15 % (р <0,05). The lumen diameter of the striated ducts was by 5,29 % significantly greater than the values in controls (р <0,05). The lumen diameter of the granular ducts reduced by 2,45 % (р <0,05). On day 30 of the experiment the height of the epithelial cells of the intercalated ducts was by 8,47 % significantly less (р <0,05), the height of the epithelial cells of the striated ducts was by 12,27 % less (р <0,05) and the height of the epithelial cells of the granular ducts was by 11,96 % less (р <0,05) than the values in controls. Conclusions: No recovery of parameters occurs by day 30 of the experiment, indicating the depletion of the secretory epithelium of the duct system, due to dystrophic changes caused by vascular disorder in the microvasculature.

scholarly journals MORPHOMETRIC DESCRIPTION OF THE EXCHANGE SEGMENT OF MICROVASCULATURE OF RATS’ SALIVARY GLANDS IN NORMAL CONDITIONS AND CHRONIC ETHANOL INTOXICATION

2019 ◽  
Vol 72 (3) ◽  
pp. 323-326 ◽  
Author(s):  
Kostyantyn V. Shevchenko ◽  
Galyna A. Yeroshenko ◽  
Оlena S. Yakushko ◽  
Kateryna S. Kazakova ◽  
Denys R. Kramarenko
Keyword(s):  
Standard Procedure ◽  
Early Observation ◽  
Chronic Ethanol ◽  
Control Group ◽  
Albino Rats ◽  
Outer Diameter ◽  
Ethanol Intoxication ◽  
Submandibular Glands ◽  
Intact Group ◽  
Chronic Ethanol Intoxication

Introduction: The state of the segments of the microvasculature, especially exchange one, has a significant effect on the organs’ functions. The results of it’s research can be objectified by morphometric method. The aim: to determine the dynamics of changes in metric indices of the exchange segment of microvasculature of rats’ submandibular glands in normal conditions and in chronic ethanol intoxication. Materials and methods: 45 albino rats were involved into study. Intact group (n=5) animals were administered with NaCl isotonic solution 4 times a day directly into the stomach. Experimental group (n=40) animals were administered with 40° ethanol 4 times a day directly into the stomach. Animals were sacrificed on 5, 9, 12 and 30 days by overdose of thiopental anesthesia. Lobules of submandibular glands were embedded into epon-812 according to standard procedure. Results: On day 5 of the experiment the outer diameter of the capillary wall significantly reduced (4,91±0,02 μm), that is 19,5 % less than the values in controls (р<0,05). On day 9 of the experiment it was 15,2 % lower than the value of control group (р>0,05). On day 30 of the experiment the outer capillary diameter was 8 % lower compared to controls (р<0,05). Conclusions: The early observation showed vasodilatation, confirmed by the constriction of the outer diameter and lumen diameter, accompanied by the thickening of the vascular wall under the influence of chronic ethanol intoxication. The indices did not come to normal values by the 30-th day of the experiment.

ULTRASTRUCTURAL REMODELING OF RAT SUBMANDIBULAR GLANDS IN CHRONIC ETHANOL INTOXICATION

2020 ◽  
Vol 16 (73) ◽  
pp. 175
Author(s):  
G.А. Yeroshenko ◽  
K.V. Shevchenko ◽  
O.D. Lisachenko ◽  
O.V. Vilhova ◽  
O.S. Yakushko ◽  
...  
Keyword(s):  
Chronic Ethanol ◽  
Ethanol Intoxication ◽  
Submandibular Glands ◽  
Chronic Ethanol Intoxication

scholarly journals Morphometric features of rat pinealocytes in conditions of chronic ethanol intoxication

2021 ◽  
Vol 27 (1) ◽  
pp. 17-22
Author(s):  
V.V. Pshychenko ◽  
V.S. Cherno
Keyword(s):  
Pineal Gland ◽  
Morphological Changes ◽  
Alcohol Intoxication ◽  
Morphometric Parameters ◽  
Chronic Ethanol ◽  
Male Rats ◽  
Control Group ◽  
Ethanol Intoxication ◽  
Chronic Ethanol Intoxication ◽  
Experimental Group

Ethanol has chronobiological effects, which are associated with inhibition of melatonin synthesis and secretion and disruption of the sleep-wake cycle. Ethanol is known to cause sleep fragmentation due to frequent awakenings, prolong wakefulness, and reduce the duration of the slow sleep phase. At the same time, changes in the morphology of the pineal gland under chronic exposure to ethanol remain poorly studied. Of particular interest are changes in the basic morphometric parameters of pinealocytes, because they are a marker of the functional state of the pineal gland. The aim of the study was to morphometrically study the features of morphological changes in rat pinealocytes in the physiological norm and in chronic ethanol intoxication. To achieve this goal, we used 20 laboratory male rats: a control group and an experimental group. The control group was under standard vivarium conditions. For the experimental group, alcohol intoxication was modeled by injecting a 40% ethanol solution at the rate of 12 mg/kg of body weight intragastric 4 times a day. The morphometric parameters of pinealocytes were studied on day 30 from the start of the study. According to the results of morphometric measurements, a significant increase in the parameters of light pinealocytes and a decrease in the parameters of dark cells were established. It was determined that the average values of the cytoplasm area of light pinealocytes increase by 54.55% (p<0.05), the area of the nucleus increases by 61.32% (p<0.05), and the area of the nucleolus by 32.84% (p<0.05) compared with the control group. The area of the cytoplasm of dark pinealocytes decreases by 27.2% (p<0.05), and the area of the nucleus by 37.33% (p<0.05). Changes in the ratio of light and dark pinealocytes were also noted. An increase in the number of active light cells by 8.17% was found. The detected morphometric changes indicate high functional activity of cells, which has a compensatory nature in response to apoptosis of pinealocytes.

Effects of the Acute and Chronic Ethanol Intoxication on Acetate Metabolism and Kinetics in the Rat Brain

2018 ◽  
Vol 42 (2) ◽  
pp. 329-337 ◽  
Author(s):  
Ya-Ju Hsieh ◽  
Liang-Chih Wu ◽  
Chien-Chih Ke ◽  
Chi-Wei Chang ◽  
Jung-Wen Kuo ◽  
...  
Keyword(s):  
Rat Brain ◽  
Chronic Ethanol ◽  
Acetate Metabolism ◽  
Ethanol Intoxication ◽  
Chronic Ethanol Intoxication

Mechanisms of Immune Status Disorders in Chronic Ethanol Intoxication

2011 ◽  
Vol 152 (1) ◽  
pp. 90-92 ◽  
Author(s):  
P. F. Zabrodskii ◽  
V. G. Lim ◽  
V. A. Grishin ◽  
A. V. Kuzmin
Keyword(s):  
Immune Status ◽  
Chronic Ethanol ◽  
Ethanol Intoxication ◽  
Chronic Ethanol Intoxication

scholarly journals Inhibition of hepatic regeneration in rats by acute and chronic ethanol intoxication

1979 ◽  
Vol 77 (3) ◽  
pp. 528-531 ◽  
Author(s):  
J.R. Wands ◽  
E.A. Carter ◽  
N.L.R. Bucher ◽  
K.J. Isselbacher
Keyword(s):  
Chronic Ethanol ◽  
Hepatic Regeneration ◽  
Ethanol Intoxication ◽  
Chronic Ethanol Intoxication

THE GAMMA-AMINOBUTYRIC ACID (GABA) SYSTEM IN BRAIN DURING ACUTE AND CHRONIC ETHANOL INTOXICATION

1975 ◽  
Vol 25 (1) ◽  
pp. 43-48 ◽  
Author(s):  
I. A. Sytinsky ◽  
B. M. Guzikov ◽  
M. V. Gomanko ◽  
V. P. Eremin ◽  
N. N. Konovalova
Keyword(s):  
Chronic Ethanol ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Ethanol Intoxication ◽  
Gaba System ◽  
Chronic Ethanol Intoxication
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