scholarly journals THE CLINICAL ROLE OF COMPONENT DIAGNOSTICS OF PEANUT ALLERGY AMONG CHILDREN WITH ALLERGIC DISEASES

2014 ◽  
Vol 11 (6) ◽  
pp. 41-46
Author(s):  
A M Shulyaeva ◽  
A N Pampura ◽  
T S Okuneva ◽  
E I Shabelnikova
Keyword(s):  
Clinical Significance ◽  
Allergic Diseases ◽  
Specific Ige ◽  
Diagnostic Significance ◽  
Clinical Role ◽  
Ara H1 ◽  
Peanut Allergens ◽  
Method Of Measurement

Background. To determine the clinical significance of IgEmediated sensibilization to recombinant peanut allergens. Materials and methods. We examined 30 children with allergic diseases and sensibilization to whole peanut allergen. All children passed through allergological examination, including determination of specific IgE (method of Measurement CAP 100 Phadia, Switzerland) to recombinant peanut allergens: Ara h1, Ara h2, Ara h3, Ara and Ara h8, h9. Results. A wide variation of combinations of sensibilization to various peanut allergens was found. Sensibilization to rAra hi allergen was found in 40% of cases, to rAra h2 - in 36,6%, to rAra h3 - in 33%, to rAra h8 - in 60%, to rAra h9 in 56,6% of cases. Their clinical significance was 36,6% (rAra hi), 26% (rAra h2), 23% (rAra h3), 33% (rAra h8), 36% (rAra h9). Monosensibilization to peanut allergens rAra hi, 8 and 9 was identified in 26,6% of cases. The interrelation of rAra h2 and rAra h8 with atopic dermatitis and rAra h3 with asthma was found. Conclusion. Component diagnostics of peanut allergens has high diagnostic significance in further administration of elimination diets.

scholarly journals Comparison of diagnostic parameters of methods for determination of sensitization to allergen D. pteronyssinus in patients with respiratory allergic diseases

2020 ◽  
pp. 19-26
Author(s):  
Artemy Ye. Bogomolov ◽  
Lyudmila I. Dubchak
Keyword(s):  
Allergic Rhinitis ◽  
Skin Testing ◽  
Allergic Diseases ◽  
Specific Ige ◽  
Test Method ◽  
Atopic Asthma ◽  
Diagnostic Significance ◽  
Western Blots ◽  
Diagnostic Parameters

The aim of the study was to evaluate the diagnostic parameters of various methods for determining allergy sensitization to the dust mite allergen Dermatophagoides pteronyssinus in patients with respiratory allergic diseases – allergic rhinitis and bronchial asthma. Materials and methods: During the study, 88 patients with allergic rhinitis and / or atopic asthma were examined with three different methods of specific allergic diagnosis (in vivo and in vitro) according to the recommendations of the ethics committee of the Pirogov Vinnitsa National Medical University, all of which were out of acute period. Inclusion criteria were a diagnosis of allergic rhinitis (both intermittent and persistent) and / or atopic asthma. The prick-test was conducted according to the classic test method by normative documents with commercial allergen extracts. Western blots for the determination of IgE levels were performed using RIDA AllergyScreen test systems (R-Biopharm AG, Darmstadt, Germany) and Euroline (Euroimmun). Results and discussion: The results of two systems for the determination of specific IgE to D. Pteronyssinus by the Rida AllergyScreen and Euroline methods have a systematic difference of values (-1.27 kU / l). There is a good agreement between the results of skin testing with D. Pteronyssinus allergens and the detection of specific IgE by the Rida AllergyScreen method, and there is a satisfactory agreement between the results of skin testing with D. Pteronyssinus allergens and detection of specific IgE by the Euroline method. According to the results of the curve of detection of specific IgE by AllergyScreen method for determination of sensitization to allergen D. Pteronyssinus has excellent diagnostic significance (AUC=0,953), detection of specific IgE by Euroline method for determination of sensitization to allergen D. Pteronyssinus – good AUC=0,7.

scholarly journals The Importance of Diet in Predicting the Remission of Urticaria—Determination of Allergen-Specific IgE

Medicina ◽  
2021 ◽  
Vol 57 (7) ◽  
pp. 679
Author(s):  
Monica Iuliana Ungureanu ◽  
Liliana Sachelarie ◽  
Radu Ciorap ◽  
Bogdan Aurelian Stana ◽  
Irina Croitoru ◽  
...  
Keyword(s):  
Predictive Value ◽  
Allergic Diseases ◽  
Specific Ige ◽  
Egg White ◽  
Food Allergens ◽  
Pediatric Hospital ◽  
Disease Remission ◽  
Specific Serum ◽  
Different Types

Background and Objectives: Different types of food introduced gradually in the diet will expose children to different food allergens, increasing the chance of developing allergic diseases. The aim of our study was to determine if allergen-specific IgE values can influence, depending on the diet, the prediction of remission of urticaria in children. Materials and Methods: This prospective study was conducted in 132 patients diagnosed over two years with urticaria, admitted to “Sf. Maria” Clinical Pediatric Hospital Iaşi. Total IgE assay was performed by ELISA, and determination of specific serum IgE by the CLA System Quanti Scan method (Innogenetics, Heiden, Germany). Data were gathered and statistical analysis was performed using statistical software SPSS, using descriptive and inferential statistics. Results: The determination of specific IgE to food allergens was performed on a total of 132 cases. The values of specific IgE were positive for one or more food allergens in 84 patients (63.64%). The most common allergens involved were: cow’s milk in 33.3% cases, egg white in 22.6% cases, and hazelnuts in 11.9% cases. The specific IgE values for the different types of food included in our study had a predictive value for disease remission. Conclusions: The determination of specific IgE confirms the presence of a particular food allergen and may have predictive value for the future development of an allergic manifestation.

scholarly journals Az ösztrogénmetabolom biológiai és klinikai jelentősége lokális folyamatokban

2017 ◽  
Vol 158 (24) ◽  
pp. 929-937
Author(s):  
Krisztián Kovács ◽  
Barna Vásárhelyi ◽  
Katalin Mészáros ◽  
Attila Patócs ◽  
Gellért Karvaly
Keyword(s):  
Biological Activity ◽  
Clinical Significance ◽  
Physiological Role ◽  
Tissue Homeostasis ◽  
Diagnostic Significance ◽  
Peripheral Tissues ◽  
Breakdown Process ◽  
Specific Manner ◽  
Related Compounds

Abstract: Considerable knowledge has been gathered on the physiological role of estrogens. However, fairly little information is available on the role of compounds produced in the breakdown process of estrone and estradiol wich may play a role in various diseases associated with estrogen impact. To date, approximately 15 extragonadal estrogen-related compounds have been identified. These metabolites may exert protective, or, instead, pro-inflammatory and/or pro-oncogenic activity in a tissue-specific manner. Systemic and local estrogen metabolite levels are not necesserily correlated, which may promote the diagnostic significance of the locally produced estrogen metabolites in the future. The aim of the present study is a bibliographic review of the extragonadal metabolome in peripheral tissues, and to highlight the role of the peripheral tissue homeostasis of estrogens as well as the non-hormonal biological activity and clinical significance of the estrogen metabolome. Orv Hetil. 2017; 158(24): 929–937.

scholarly journals CLINICAL SIGNIFICANCE OF DETERMINATION OF IGE ANTIBODIES TO STAPHYLOCOCCUS AUREUS AND MALASSEZIA SPECIES IN SERUM OF CHILDREN WITH ATOPIC DERMATITIS

2018 ◽  
Vol 63 (4) ◽  
pp. 84-88
Author(s):  
T. S. Okuneva ◽  
M. V. Kushnareva ◽  
A. N. Pampura ◽  
E. E. Varlamov ◽  
N. G. Konyukova
Keyword(s):  
Staphylococcus Aureus ◽  
Atopic Dermatitis ◽  
Clinical Significance ◽  
Specific Ige ◽  
Frequency Of Occurrence ◽  
Ige Antibodies ◽  
Malassezia Species ◽  
Specific Ige Antibodies ◽  
Malassezia Spp

We studied the frequency of occurrence and concentration of allergen-specific IgE antibodies (asIgE) to enterotoxins A, B, C and TSST-1 of Staphylococcus aureus and Malassezia spp. in the serum of children with atopic dermatitis in the acute period of the disease. The determination of asIgE levels was performed in 84 children aged from 5 months to 17 years using the immunochemiluminescent method on the UniCAP 100 analyzer (Phadia, Sweden). The level of asIgE was evaluated on a scale of 6 classes.The sensitization to S. aureus enterotoxins was detected in 29 children with аsIgE concentration from 0.35 to 25.8 kU/L (I–IV classes). In 16 children were observed 8 polysensitization options to S. aureus allergens. Combinations of asIgE to A + B and A + B + C enterotoxins were most common – in every fourth and fifth child,respectively. Sensitization to Malassezia spp. was observed in 41 children with concentration from 0.38 to 98.9 KU/L (I–V classes). Polysensitization to both S. aureus and Malassezia spp. allergens was observed in one of two children. A higher degree of sensitisation to Malassezia spp allergens has been established compared to S. aureus.Conclusion. To improve the specific diagnostics of atopic dermatitis, it is advisable to examine the serum for a complete panel of recombinant S. aureus enterotoxins in combination with the determination of asIgE to Malassezia spp. 

Simultaneous determination of major peanut allergens Ara h1 and Ara h2 in baked foodstuffs based on their signature peptides using ultra-performance liquid chromatography coupled to tandem mass spectrometry

2019 ◽  
Vol 11 (12) ◽  
pp. 1689-1696 ◽  
Author(s):  
Jingshun Zhang ◽  
Yuwei Hong ◽  
Zengxuan Cai ◽  
Baifen Huang ◽  
Junlin Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Tandem Mass Spectrometry ◽  
Ultra Performance Liquid Chromatography ◽  
Tandem Mass ◽  
Ara H1 ◽  
Peanut Allergens ◽  
Signature Peptides

A robust UPLC-MS/MS method was established for the quantitative analysis of the major peanut allergens Ara h1 and Ara h2 in baked foodstuffs based on their signature peptides.

scholarly journals The atopic desease development in infants and preschool children in Lesnoy town of Sverdlovsk region

2015 ◽  
Vol 12 (2) ◽  
pp. 59-63
Author(s):  
N A Volkova ◽  
I A Tuzankina ◽  
V N Shershnev
Keyword(s):  
Preschool Children ◽  
Clinical Symptoms ◽  
Allergic Diseases ◽  
Specific Ige ◽  
Atopic Diseases ◽  
Different Types ◽  
Specific Ige Antibodies ◽  
Airborne Allergens

The aim of study. To determine the structure of atopic diseases and characterization of different allergens in their progression in infants and preschool children in Lesnoy town. Material and methods. We studied a spectrum of allergen-specific IgE antibodies in children’s blood with different allergic diseases. Comparative analysis of the results was conducted with methods of mathematical statistics. Results. Our data demonstrate the different stages of formation of atopic diseases in children. We revealed that clinical symptoms of atopic diseases and relevance of allergens were changing gradually as children were getting older. Our results showed that the role of nutrition allergens is reduced while airborne allergens came to the fore. Conclusion. Formation and progression of atopic diseases are determined by various allergens. The temporary relevance of different types of allergens indicates a diversity of multivalent sensibilization during affection of the «shock» organ; the result of this phenomenon appears as atopic march. Detection the relevant causative allergens is important for prevention of progression of allergic diseases.

scholarly journals Features of sensitization to widespread allergens in residents of St. Petersburg and the Leningrad region with common allergic deseases

2013 ◽  
Vol 10 (4) ◽  
pp. 74-80
Author(s):  
O V Aak ◽  
A V Sobolev
Keyword(s):  
Allergic Rhinitis ◽  
Atopic Dermatitis ◽  
Bronchial Asthma ◽  
Allergic Diseases ◽  
Specific Ige ◽  
Leningrad Region ◽  
Food Allergens ◽  
Significant Similarity ◽  
High Degree

Background. To identify differences in sensitization to widespread allergens in patients with common allergic diseases: allergic rhinitis, bronchial asthma, angioedema, allergic urticaria and atopic dermatitis. Materials and methods. Determination of sIgE in serum using commercial and domestic reagent kits («AllergoI-FA-specific IgE» and biotinylated allergens of «Alcor Bio», St. Petersburg) was conducted in patients with allergic rhinitis, bronchial asthma, angioedema, allergic urticaria and atopic dermatitis. Results. There was a significant similarity between the spectrum of sensitization in patients with allergic rhinitis and asthma In atopic dermatitis patients the increasing of frequency of food and especially mold sensitization was estimated. The high degree of correlation between the moulds, plants and food allergens in atopic dermatitis was observed. Conclusion. The results obtained in the study can be interesting for physicians in allergological examination and for the researchers to identify new cross-reactive allergens.

scholarly journals Features of clinical manifestations of allergic diseases in children sensitized to potatoes

2011 ◽  
Vol 8 (4) ◽  
pp. 55-61
Author(s):  
N G Konyukova ◽  
A N Pampura ◽  
E S Fedenko ◽  
A I Khavkin ◽  
T S Okuneva ◽  
...  
Keyword(s):  
Severe Disease ◽  
Clinical Manifestations ◽  
Allergic Diseases ◽  
Specific Ige ◽  
Food Plant ◽  
Plant Proteins ◽  
Ige Antibodies ◽  
Specific Ige Antibodies

Background. To establish the clinical and immunological features of the manifestations of allergic diseases in children with IgE-mediated sensitization to potato. Methods. allergy tests (skin prick tests and / or the determination of specific IgE in serum by ImmunoCap, Phadia was performed in 171 children with allergic diseases, who had sensitization to food plant proteins. Results. sensitization to the potato found in 134 children. significant discrepancies (36%) in the results of in vitro and in vivo tests to the potatoes were shown. sensitization to the potatoes is more common in patients with atopic dermatitis (ad), especially in severe disease. among children with the level of specific IgE antibodies to potato ≥2 kUa / l there were more common patients with severe ad. the correlation between the level of specific IgE antibodies to the potatoes and some of food plant proteins was found. the relationship between the concentration of specific IgE to the potatoes and Bet v 2 was not detected. Conclusion. the presence and concentration of specific IgE to the potatoes to a certain extent associated with clinical manifestations of ad. the data obtained substantiate the determination of specific IgE antibodies to potato in children with ad, especially in severe cases.

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