scholarly journals Classification of anemia as to the medullary response through reticulocyte count in dogs attended to in the Veterinary Hospital from Federal University of Mato Grosso, Campus Sinop.

2020 ◽  
Vol 13 (6) ◽  
pp. 79
Author(s):  
D. B. Morais ◽  
M. S. Jesus ◽  
A. C. Bonatto ◽  
D. W. Silva ◽  
A. Kataoka
Keyword(s):  
Tissue Oxygenation ◽  
Reticulocyte Count ◽  
Hemoglobin Concentration ◽  
Exercise Intolerance ◽  
Mato Grosso ◽  
Blood Smears ◽  
Null Response ◽  
Veterinary Hospital ◽  
Supravital Staining

Anemia is defined as the presence of erythrocytes, hemoglobin concentration and/or hematocrit below the reference values, leading to death if not treated. It is manifested clinically in the presence of pale mucosae, dyspnea, exercise intolerance and increased heart rate due to reduced tissue oxygenation. The aim of this study was to classify the anemia according to the medullar response in regenerative with weak, moderate or intense release and non-regenerative with null response, correlating with its possible causes by reticulocyte count. Thus, 50 blood samples from anemic dogs treated at the Veterinary Hospital of the Universidade Federal de Mato Grosso, Sinop Campus, were evaluated. After collection, blood smears were prepared for reticulocyte counting using supravital staining. Of the 50 animals evaluated, 54% (27/50) showed non-regenerative anemia with null response (<60,000 cells/μL), 36% (18/50) showed regeneration with weak release (60,000 to 150,000 cells/μL) and 10% (5/50) presented moderate to intense regeneration (>150,000 cells/μL). In conclusion, in this study was observed a predominance of non-regenerative anemia and a lower incidence of responsive anemia; the medullary response was more evident in hemoparasitosis anemia and non-regenerative anemia occurred mainly in animals with renal insufficiency, possibly due to insufficient production of erythropoietin

scholarly journals Hereditary Spherocytosis : A Clinical Experience

2019 ◽  
Vol 28 (1-2) ◽  
pp. 27-35
Author(s):  
F. X. Santoso ◽  
Sukardi Sukardi ◽  
Bambang Permono ◽  
Netty R. H. T. ◽  
S. Untario
Keyword(s):  
Hereditary Spherocytosis ◽  
Reticulocyte Count ◽  
Hemoglobin Concentration ◽  
The Past ◽  
Northern European ◽  
Blood Smears ◽  
The Mean ◽  
Almost All ◽  
Peripheral Blood Smears

Although hereditary spherocytosis is a common cause of hemolytic anemia among whites of Northern European descent, it is uncommon in Asia. In the past 8.5 years (from December 1978 to June 1987), we found only six cases of hereditary spherocytosis. They were 3 males and 3 females, and their ages ranged from 3 months to 8 years, with a mean age of 3.3 years. The most common presenting complaint was anemia (6 cases) followed by jaundice (4 cases) and splenomegaly (4 cases). Other symptoms were fever, abdominal pain and hepatomegaly. The mean hemoglobin concentration of these patients was 7.5 g/dl, in which 2 patients had severe anemia (less than 6 g/dl). Reticulocyte count ranged from 1.9% to 10% (mean 5.9%). All patients were found to have spherocytosis in their peripheral blood smears and an increased red blood cell fragility. Splenectomy was performed in one patient. There was no significant complication after operation in a 7-month follow up. The clinical manifestation returned to normal and the mean hemoglobin concentration increased. The existence of hereditary spherocytosis could not be proven in almost all parents of the patients. Based on this fact, is 'congenital spherocytosis' a more suitable term instead of hereditary spherocytosis?

Performance of the CellaVision DC-1 digital cell imaging analyser for differential counting and morphological classification of blood cell

2021 ◽  
pp. jclinpath-2021-207863
Author(s):  
Lisa N van der Vorm ◽  
Henriët A Hendriks ◽  
Simone M Smits
Keyword(s):  
Blood Cell ◽  
Peripheral Blood ◽  
Cell Imaging ◽  
Small Satellite ◽  
Morphological Classification ◽  
Clinical Sensitivity ◽  
Blood Smears ◽  
Low Volume ◽  
Peripheral Blood Smears

AimsRecently, a new automated digital cell imaging analyser (Sysmex CellaVision DC-1), intended for use in low-volume and small satellite laboratories, has become available. The purpose of this study was to compare the performance of the DC-1 with the Sysmex DI-60 system and the gold standard, manual microscopy.MethodsWhite blood cell (WBC) differential counts in 100 normal and 100 abnormal peripheral blood smears were compared between the DC-1, the DI-60 and manual microscopy to establish accuracy, within-run imprecision, clinical sensitivity and specificity. Moreover, the agreement between precharacterisation and postcharacterisation of red blood cell (RBC) morphological abnormalities was determined for the DC-1.ResultsWBC preclassification and postclassification results of the DC-1 showed good correlation compared with DI-60 results and manual microscopy. In addition, the within-run SD of the DC-1 was below 1 for all five major WBC classes, indicating good reproducibility. Clinical sensitivity and specificity were, respectively, 96.7%/95.9% compared with the DI-60% and 96.6%/95.3% compared with manual microscopy. The overall agreement on RBC morphology between the precharacterisation and postcharacterisation results ranged from 49% (poikilocytosis) to 100% (hypochromasia, microcytosis and macrocytosis).ConclusionsThe DC-1 has proven to be an accurate digital cell imaging system for differential counting and morphological classification of WBCs and RBCs in peripheral blood smears. It is a compact and easily operated instrument that can offer low-volume and small satellite laboratories the possibilities of readily available blood cell analysis that can be stored and retrieved for consultation with remote locations.

scholarly journals Molecular diagnosis of Hepatozoon canis in symptomatic dogs in the city of Goiania, Goiás, Brazil

2016 ◽  
Vol 68 (6) ◽  
pp. 1431-1439
Author(s):  
S.C. Duarte ◽  
J.A. Parente ◽  
O.J. Silveira Neto ◽  
V.S. Jayme ◽  
T.S.A. Bastos ◽  
...  
Keyword(s):  
Laboratory Diagnosis ◽  
Extraction Process ◽  
Hepatozoon Canis ◽  
Phylogenetic Study ◽  
Blood Smears ◽  
Two Samples ◽  
Pcr Products ◽  
Hepatozoon Americanum ◽  
The City ◽  
Veterinary Hospital

ABSTRACT More than 300 species have been described in the genus Hepatozoon, occurring in different vertebrates. Among these, only Hepatozoon canis and Hepatozoon americanum are seen in dogs. Different methods may be used for laboratory diagnosis. The most common of these is direct parasitological examination of parasite stages in blood smears. The aim of this investigation was to conduct a phylogenetic study on Hepatozoon isolates from symptomatic dogs in the city of Goiânia, Goiás, Brazil. Blood samples were obtained from 40 symptomatic dogs that had been referred to the Veterinary Hospital of the Federal University of Goiás. Among these, only two samples were positive for Hepatozoon spp. using the direct parasitological method. These samples were then subjected to a DNA extraction process and amplification of a fragment of the 18S rRNA by means of PCR. Subsequently, the PCR products from each sample were purified and sequenced. The sequences obtained were then analyzed using the BLASTn algorithm, which identified both sequences of this study as Hepatozoon canis. By applying the Mega4 software, it was confirmed that these isolates of H. canis from dogs in Goiânia are similar to other reference isolates of the same species from other regions of Brazil and worldwide.

scholarly journals Endoparasites of wild animals from three biomes in the State of Mato Grosso, Brazil

2016 ◽  
Vol 68 (3) ◽  
pp. 571-578 ◽  
Author(s):  
D.G.S. Ramos ◽  
A.R.G.L.O. Santos ◽  
L.C. Freitas ◽  
S.H.R. Correa ◽  
G.V. Kempe ◽  
...  
Keyword(s):  
Host Species ◽  
Abiotic Factors ◽  
Wild Animals ◽  
New Host ◽  
Biotic Factor ◽  
Mato Grosso ◽  
Biotic And Abiotic Factors ◽  
Parasitological Examination ◽  
Biotic Potential ◽  
Veterinary Hospital

ABSTRACT The population of wild animals is regulated by many biotic and abiotic factors, and parasites are a biotic factor that affects the dynamic and density of host populations. From 2002 to 2014, 62 wild animals from the biomes Pantanal, Amazon, and "Cerrado" (or Savanna), which died in attendance in the veterinary hospital or have been road-killed, underwent necropsy for parasitological examination. Overall, 36 species of parasites were identified from 24 host species. Among the parasites, the most prevalent order was Oxyurida (29.1%), followed by Strongylida (20.9%), Spirurida (19.4%), Ascaridida (16.2%), Pentastomida (3.2%), Echinostomida (3.2%), Gygantorhynchia (3.2%), Rhabditida (1.6%), Plagiorchiida (1.6%), and Monilimorfida (1.6%), especially nematodes, which have more biotic potential and is more easily adapted to the environment than other classes. The occurrence of endoparasites was observed more frequently in endothermic than ectothermic animals, and herein is reported eleven new host occurrences for endoparasites in wild animals. The study has contributed to the knowledge on the biodiversity of parasites in wild animals from three biomes in central-western Brazil.

scholarly journals First record of Trypanosoma sp. (Protozoa: Kinetoplastida) in tuvira (Gymnotus aff. inaequilabiatus) in the Pantanal wetland, Mato Grosso do Sul State, Brazil

2011 ◽  
Vol 20 (1) ◽  
pp. 85-87 ◽  
Author(s):  
Santiago Benites de Pádua ◽  
Márcia Mayumi Ishikawa ◽  
Fabiana Satake ◽  
Gabriela Tomas Jerônimo ◽  
Fabiana Pilarski
Keyword(s):  
Prevalence Rate ◽  
First Record ◽  
Diagnostic Methods ◽  
Mato Grosso ◽  
First Report ◽  
Paraguay River ◽  
Blood Smears ◽  
Mato Grosso Do Sul ◽  
Pantanal Wetland ◽  
Blood Centrifugation

The blood infection by Trypanosoma sp. in tuvira (Gymnotus aff. inaequilabiatus) from the Pantanal wetland was reported in this study. Ten fish from the Paraguay River in the Pantanal were evaluated for the presence of hemoflagellates. Trypomastigotes of Trypanosoma sp. were observed in blood smears from three fish (30% prevalence) and some forms were seen to be undergoing division. Using the diagnostic methods of fresh examination and blood centrifugation in hematocrit capillary tubes, the prevalence rate was 80%. This is the first report of Trypanosoma sp. in tuvira in Brazil.

scholarly journals Clinico – Hemato and Biochemical Study of Anemia in Cachectic Cattle in Baghdad

2011 ◽  
Vol 8 (4) ◽  
pp. 877-886
Author(s):  
Baghdad Science Journal
Keyword(s):  
Foreign Body ◽  
Muscular Atrophy ◽  
Biochemical Study ◽  
Hypochromic Anemia ◽  
Gastrointestinal Parasites ◽  
Blood Parasites ◽  
Blood Smears ◽  
Wbc Count ◽  
Clinical Cases

The study was carried out in order to evaluate clinically and laboratory cachectic animals suffering from anemia. Animal examined were 50 cow and calf. The study include clinical, hemato and biochemical test for accurate diagnosis of cachexia in cows and calves . Blood smears were conducted for detection of blood parasites , fecal examination for gastrointestinal parasites and Different parameters were applied for classification of cachexia , depending on bony projection specially ribs and pelvic and generalized muscular atrophy. However , The study revealed an incidence of cachexia and anemia of blood parasites was including Theileria, Anaplasma, gastrointestinal parasites, ten cases were shown foreign body syndrome while other tens were diagnosed as other clinical cases. A significant decrease ( P < 0.05) of RBCS, Hb, PCV and WBC count in debility animals were observed . However , a significant lymphocytosis were seen in blood parasites infection and other clinical cases , Neutrophilia in foreign body syndrome and other clinical cases , Esenophilia in gastrointestinal parasites infestation ,Monocytosis in all clinical cases were detected . Different parameters were applied for classification of anemia mainly morphological classification including macrocytic hypochromic anemia mainly recorded in blood parasites infection and other clinical cases while Normocytic hypochromic anemia appeared in gastrointestinal parasites infestation, and foreign body Syndrome.Fecal examination of cachectic animal indicate the identification of (7) species of gastrointestinal parasites including: Cooperia onchophora, Haemonchus contortus, Bunostomum phlebtomum, Ostertagia spp., Oesophagostomum radiatum, Trichostrongylus axei and Strongyloides papillosus. However , a mixed infestation parasite was dominant and recorded in Animal.Biochemical changes revealed a. Hypoprotenemia appears in all cachectic animal except in foreign body syndrome cases .

scholarly journals Natural hemoplasma infection of cats in Cuiaba, Mato Grosso, Brazil

2018 ◽  
Vol 39 (2) ◽  
pp. 875 ◽  
Author(s):  
Herica Makino ◽  
Daphine Ariadne Jesus de Paula ◽  
Valéria Regia Franco Sousa ◽  
Adriane Jorge Mendonça ◽  
Valéria Dutra ◽  
...  
Keyword(s):  
Blood Count ◽  
Clinical Care ◽  
Mato Grosso ◽  
Blood Samples ◽  
Factors Associated ◽  
Mixed Breed ◽  
The City ◽  
Veterinary Hospital ◽  
Candidatus Mycoplasma Turicensis

The aim of this research was to investigate natural hemoplasma infection in cats treated at the Veterinary Hospital of the Federal University of Mato Grosso, and the factors associated with infection. Blood samples from 151 cats of different sexes, breeds, and ages were analyzed by PCR and blood count. The overall occurrence of hemoplasma was 25.8%. Mycoplasma haemofelis (Mhf), ‘Candidatus Mycoplasma haemominutum (CMhm)’, and ‘Candidatus Mycoplasma turicensis’ (CMt) were observed in 15.2%, 14.6% and 2.6% of cats, respectively. In 6.6 % of cases, co-infection was observed. Male felines or mixed breed cats were associated with infection by CMhm (P = 0.02 and 0.04, respectively). The data obtained demonstrated an occurrence of 25.8% for hemoplasma infection in felines coming from clinical care in the city of Cuiabá, where males were at higher risk of acquiring the infection by these agents, in addition to a higher risk for CMhm in felines with no specific breed.

scholarly journals Molecular detection of feline arthropod-borne pathogens in cats in Cuiabá, state of Mato Grosso, central-western region of Brazil

2013 ◽  
Vol 22 (3) ◽  
pp. 385-390 ◽  
Author(s):  
Natasha Gandolfi Miceli ◽  
Fernando Antonio Gavioli ◽  
Luiz Ricardo Gonçalves ◽  
Marcos Rogério André ◽  
Valéria Régia Franco Sousa ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Western Region ◽  
Mato Grosso ◽  
Animal Shelters ◽  
Blood Samples ◽  
Cytauxzoon Felis ◽  
The City ◽  
Veterinary Hospital

Hemotrophic mycoplasmas (hemoplasmas), Bartonellasp., Hepatozoon sp. and Cytauxzoon felis are prominent pathogens that circulate between cats and invertebrate hosts. The present study aimed to detect the presence of DNA from hemoplasmas,Bartonella sp., Hepatozoon sp. andCytauxzoon felis, and then confirm it by means of sequencing, in blood samples from cats in Cuiabá, MT, Brazil. From February 2009 to February 2011, blood samples with added EDTA were collected from 163 cats that were being housed in four different animal shelters in the city of Cuiabá, state of Mato Grosso, Brazil and from 15 cats that were admitted to the veterinary hospital of the Federal University of Mato Grosso (UFMT). Out of the 178 cats sampled, 15 (8.4%) were positive for hemoplasmas: four (2.2%) forMycoplasma haemofelis, 12 (6.7%) for ‘Candidatus M. haemominutum’ and one (0.5%) for ‘Candidatus M. turicensis’. One cat (0.5%), a patient that was attended at the veterinary hospital, was coinfected with M. haemofelis, ‘Candidatus M. haemominutum’ and ‘Candidatus M. turicensis’, based on sequencing confirmation. Four cats were positive for Bartonella spp.: three (1.7%) for B. henselae and one (0.5%) for B. clarridgeiae. None of the animals showedCytauxzoon sp. or Hepatozoon sp. DNA in their blood samples. This study showed that cats housed in animal shelters in the city of Cuiabá, state of Mato Grosso, are exposed to hemoplasmas andBartonella species.

TISSUE OXYGENATION IN PATIENTS WITH LOW HEMOGLOBIN CONCENTRATION DURING CARDIAC SURGERY; COMPARISON OF TWO METHODS

1998 ◽  
Vol 86 (Supplement) ◽  
pp. 52S
Author(s):  
Y Ates ◽  
N Canakci ◽  
J Hallioglu ◽  
B Aygunes ◽  
A Aslan ◽  
...  
Keyword(s):  
Cardiac Surgery ◽  
Tissue Oxygenation ◽  
Hemoglobin Concentration

scholarly journals Hematological values associated to the serological and molecular diagnostic in cats suspected of Ehrlichia canisinfection

2013 ◽  
Vol 22 (4) ◽  
pp. 470-474 ◽  
Author(s):  
ísis Assis Braga ◽  
Luana Gabriela Ferreira dos Santos ◽  
Andréia Lima Tomé Melo ◽  
Felipe Wolf Jaune ◽  
Thaysa Felfili Ziliani ◽  
...  
Keyword(s):  
Molecular Diagnostic ◽  
Rrna Gene ◽  
Igg Antibody ◽  
Mato Grosso ◽  
Pcr Products ◽  
Antibody Titers ◽  
Polymerase Chain ◽  
Pcr Targeting ◽  
Veterinary Hospital ◽  
Hematological Alterations

The literature contains several studies on feline ehrlichiosis. However, information about the characteristics of Ehrlichiainfection in cats is still scanty. This study evaluated the association between Ehrlichia spp. infection and the hematologic data of 93 cats treated at the Federal University of Mato Grosso Veterinary Hospital in Cuiabá, state of Mato Grosso, Brazil. The presence of or exposure to Ehrlichia spp. infection was evaluated by Polymerase Chain Reaction (PCR) targeting the dsb and 16S rRNA gene of Ehrlichia, and by detection of anti-Ehrlichia canis IgG antibodies in Indirect Fluorescence Assay (IFA), respectively. Eight (8.6%) cats tested positive by PCR and the partial DNA sequence obtained from PCR products was a 100% match to E. canis. Forty-two (45.1%) cats showed antibody reactivity against Ehrlichia spp. Hematological alterations such as low erythrocyte count, thrombocytopenia, lymphopenia and monocytosis were observed in PCR positive cats. Among them, low erythrocyte counts were associated with IgG antibody titers of 40 to 640 and five cats also tested positive by PCR. Furthermore, PCR-positive cats showed a tendency to be lymphopenic. No correlation was found between age and sex, and no ticks were observed in any of the examined cats.

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