scholarly journals Isolation of antioxidants from Alchemilla xanthochlora

2021 ◽  
Vol 9 (3) ◽  
Author(s):  
Miroslav Ondrejovič ◽  
Zuzana Ondrigová ◽  
Janka Kubincová
Keyword(s):  
Column Chromatography ◽  
Plant Material ◽  
Liquid Extraction ◽  
Photometric Method ◽  
Herbal Extracts ◽  
Isopropanol Extract ◽  
Liquid Liquid Extraction ◽  
Tlc Analysis ◽  
Solid Liquid ◽  
Chloroform Methanol

The aim of this study was to find conditions for isolation of antioxidants from Alchemilla xanthochlora. The solid-liquid extraction of plant material with different solvents (n-hexane, chloroform, ethylacetate, methanol, and water) was used for preparation of crude extracts of antioxidants. For additional purification of crude extract, liquid-liquid extraction in different organic solvents (chloroform, ethylacetate, methanol) and water, and column chromatography with silica gel as stationary phase and mixture of chloroform : methanol as mobile phase were used. The content of active compounds was monitored by photometric method and special TLC analysis using DPPH as basic reagent. TLC-DPPH analysis of herbal extracts showed that antioxidants from Alchemilla xanthochlora were separated into two groups with different polarity. Finally, for the isolation of antioxidants from Alchemilla xanthochlora were successfully used the following procedures: extraction of dried grounded plant material with methanol at 50°C during 1 hour, subsequent liquid-liquid extraction in isopropanol and water and fractionation of isopropanol extract by column chromatography. Fraction with isolated antioxidants showed antioxidant activity 535.2 mg to g of fraction residue.

scholarly journals Solid-liquid separation after liquid-liquid extraction

1969 ◽  
Vol 18 (3) ◽  
pp. 398-399 ◽  
Author(s):  
Taitiro FUJINAGA ◽  
Toru KUWAMOTO ◽  
Eiichiro NAKAYAMA ◽  
Masatada SATAKE
Keyword(s):  
Liquid Extraction ◽  
Liquid Separation ◽  
Liquid Liquid Extraction ◽  
Solid Liquid ◽  
Solid Liquid Separation

scholarly journals Identification of Phytochemicals from the Water Extract of Eurycoma longifolia Roots using Solid-Liquid and Liquid-Liquid Extraction Based Fractionation Techniques

2021 ◽  
Vol 68 (4) ◽  
pp. 765-772
Author(s):  
Lee Suan Chua ◽  
Abirame Segaran ◽  
Hoi Jin Wong
Keyword(s):  
Ethyl Acetate ◽  
Solid Phase ◽  
Water Extract ◽  
Reversed Phase ◽  
Liquid Extraction ◽  
Acetone Precipitation ◽  
Eurycoma Longifolia ◽  
Liquid Liquid Extraction ◽  
Saponin Content ◽  
Solid Liquid

Phytochemicals in the water extract of Eurycoma longofolia roots were identified using both solid-liquid and liquid-liquid extraction based fractionation techniques. A reversed phase C18 solid phase extraction (SPE) was used as solid-liquid extraction, whereas solvent partition was applied as liquid-liquid extraction. Total saponin was increased after fractionation. A few known quassinoids; eurycomanone, 13α(21)-epoxyeurycomanone, pasakbumin D, 13β,18-dihydroeurycomanol and 13β,21-dihydroxyeurycomanol were identified from the 40% and 60% methanol fractions of SPE. Solvent partition extract using ethyl acetate was found to have the highest saponin content compared to butanol and chloroform fractions. Subsequent acetone precipitation of the organic fractions recovered a formylated hexose trimer and other saccharide-containing compounds. Ethyl acetate effectively recovered saponins from E. longofolia water extract using liquid-liquid extraction followed by acetone precipitation.

scholarly journals Highly Efficient, Tripodal Ion-Pair Receptors for Switching Selectivity between Acetates and Sulfates Using Solid–Liquid and Liquid–Liquid Extractions

2020 ◽  
Vol 21 (24) ◽  
pp. 9465
Author(s):  
Marta Zaleskaya ◽  
Łukasz Dobrzycki ◽  
Jan Romański
Keyword(s):  
Binding Sites ◽  
Liquid Extraction ◽  
Ion Pair ◽  
Anion Binding ◽  
Binding Modes ◽  
Anion Receptor ◽  
X Ray ◽  
Liquid Liquid Extraction ◽  
Solid Liquid Extraction ◽  
Solid Liquid

A tripodal, squaramide-based ion-pair receptor 1 was synthesized in a modular fashion, and 1H NMR and UV-vis studies revealed its ability to interact more efficiently with anions with the assistance of cations. The reference tripodal anion receptor 2, lacking a crown ether unit, was found to lose the enhancement in anion binding induced by presence of cations. Besides the ability to bind anions in enhanced manner by the “single armed” ion-pair receptor 3, the lack of multiple and prearranged binding sites resulted in its much lower affinity towards anions than in the case of tripodal receptors. Unlike with receptors 2 or 3, the high affinity of 1 towards salts opens up the possibility of extracting extremely hydrophilic sulfate anions from aqueous to organic phase. The disparity in receptor 1 binding modes towards monovalent anions and divalent sulfates assures its selectivity towards sulfates over other lipophilic salts upon liquid–liquid extraction (LLE) and enables the Hofmeister bias to be overcome. By changing the extraction conditions from LLE to SLE (solid–liquid extraction), a switch of selectivity from sulfates to acetates was achieved. X-ray measurements support the ability of anion binding by cooperation of the arms of receptor 1 together with simultaneous binding of cations.

scholarly journals Nanomicellar Extraction of Polyphenols—Methodology and Applications Review

2021 ◽  
Vol 22 (21) ◽  
pp. 11392
Author(s):  
Paweł Śliwa ◽  
Karolina Śliwa
Keyword(s):  
Extraction Method ◽  
Plant Material ◽  
Liquid Extraction ◽  
Alternative Methods ◽  
Theoretical Principle ◽  
Current State ◽  
Solid Liquid Extraction ◽  
Solid Liquid ◽  
Active Substances ◽  
Selection Of

The selection of the appropriate extraction method is crucial, especially for the receiving of active substances from plant material. The extraction using supercritical liquids and micellar-mediated extraction (MME) is the most advantageous among the alternative methods to classical solid–liquid extraction. However, the latter seems to be the best solution when the desired actives are polar. The following article presents a comprehensive review of the micellar-mediated extraction method in the last decade. The theoretical principle of the process was also refreshed and the current state of knowledge on the applications for analytical and manufacturing purposes was summarized.

Isolation of atropine and scopolamine from plant material using liquid-liquid extraction and EXtrelut® columns

2017 ◽  
Vol 1043 ◽  
pp. 202-208 ◽  
Author(s):  
Paula Śramska ◽  
Artur Maciejka ◽  
Anna Topolewska ◽  
Piotr Stepnowski ◽  
Łukasz P. Haliński
Keyword(s):  
Plant Material ◽  
Liquid Extraction ◽  
Liquid Liquid Extraction
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