scholarly journals CONTAMINATION OF CHICKEN EGGSHELLS AND EGG CONTENTS WITH SALMONELLA SPECIES FROM SELECTED FARMS IN KOSGAMA, COLOMBO DISTRICT

2021 ◽  
pp. e307
Author(s):  
Ureshika Sharuni Ambagas Ange Gedara ◽  
Fathima Sumaiya Mohamed Raafi ◽  
Dinushini Nammida Ekanayake Mudiyanselage ◽  
Shirani Chandrasiri Nambage ◽  
Piyumali Sandareka Arachchi Mudiyanselage
Keyword(s):  
Brilliant Green ◽  
Egg Quality ◽  
Foodborne Disease ◽  
Biochemical Tests ◽  
Gram Staining ◽  
Significant Difference ◽  
Peptone Water ◽  
Commercial Farms ◽  
Chicken Eggs

Salmonellosis is a common, widely distributed foodborne disease. Consumption of raw or undercooked chicken eggs infected with Salmonella has been reported in association with salmonellosis cases; however, minimum attention has been paid to regulate the quality of eggs released for consumption. This study aimed to investigate the presence of Salmonella in eggs collected from selected farms from Kosgama area and to compare the egg quality of backyard and commercial farms. Randomly purchased eggs from selected chicken farms were analyzed for the presence of Salmonella. Egg content was mixed thoroughly, and 25.0mL was inoculated into 225.0mL of 1% Buffered Peptone Water (BPW) and incubated at 350C (24h). From the pre-enriched specimen, 0.1ml was added to 10.0ml of Rappaport Vassiliadis broth and incubated at 420C (24h). The same procedure was followed for shells. Isolated cultures were streaked on Brilliant Green Agar (BGA) and Xylose Lysine Deoxycholate Agar (XLD) and incubated at 350C for 24h. Colonies were investigated with Gram staining, biochemical tests and serotyping was carried out to identify the species. Of 78 eggs, 35 were from backyard and 43 from commercial farms. Six specimens (4 from shell and 2 from content) yielded Salmonella (7.69 %). Four of the positive specimens were from backyard farms (4/35, 8.91%) and remaining two (2/43, 3.62%) were from commercial farms. Isolates were identified as S. Typhimurium and S. Enteriditis.  The prevalence of Salmonella was 7.69 % (n=6). The proportion of Salmonella showed no significant difference (p=0.782) between backyard and commercial farms.

scholarly journals Isolation, identification and antibiogram profile of Aeromonas hydrophila from broiler chickens in Mymensingh Sadar, Bangladesh

2020 ◽  
Vol 4 (1) ◽  
pp. 22-30
Author(s):  
Basana Sarker ◽  
Mohammad Arif ◽  
Nilofa Eashmen ◽  
Mir Rowshan Akter ◽  
SM Lutful Kabir
Keyword(s):  
Aeromonas Hydrophila ◽  
Broiler Chickens ◽  
Susceptibility Testing ◽  
Specific Detection ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Alkaline Peptone Water ◽  
Sensitivity Pattern ◽  
Peptone Water

Investigation of Aeromonas hydrophila was conducted to assess the microbial quality of broiler chickens from July to November 2019. A total of 60 samples from 20 broiler chickens were collected from two different locations of Mymensingh Sadar: KR market, Bangladesh Agricultural University (BAU) and Shesh mor bazar (10 birds from each location). Samples included 20 skins, 20 legs and 20 breast samples from 20 broiler chickens. PCR was done for the specific detection of each isolate and finally antimicrobial susceptibility testing was performed to check sensitivity pattern of each isolate. Alkaline peptone water was used for processing and enrichment of the samples followed by inoculation onto Aeromonas selective agar supplemented with ampicillin for the isolation and identification of A. hydrophila. Out of these 60 samples, 27 isolates were confirmed as A. hydrophila through biochemical tests and PCR where 55.56% isolates were recovered from Shesh mor market and other 44.4% isolates from KR market, BAU. Source-wise analysis revealed that maximum isolates of A. hydrophila were recovered from skin (59.26 %) followed by leg (22.22 %) and breast samples (18.52 %). PCR test revealed that all 27 isolates were found carrying lip gene which is specific for A. hydrophila. Isolates of A. hydrophila were found sensitive to ciprofloxacin (92%), gentamycin (66%) and chloramphenicol (50%); intermediate against erythromycin (50%), tetracycline (50%) and imipenem (50%); resistant against co-trimoxazole (84%) and ampicillin (100%). From the present study, it was found that samples were considerably contaminated with Aeromonas hydrophila causing risks for public health. Necessary control actions should be taken in every steps of production, processing and marketing for mitigation of this contamination. Asian Australas. J. Food Saf. Secur. 2020, 4 (1), 22-30

scholarly journals PENGARUH LAMA PERENDAMAN DALAM GEL LIDAH BUAYA (Aloe vera) TERHADAP KUALITAS TELUR AYAM RAS

ZOOTEC ◽  
2020 ◽  
Vol 40 (1) ◽  
pp. 150
Author(s):  
Asrul Lamarang ◽  
A. Yelnetty ◽  
L.Ch.M Karisoh ◽  
N.N Lontaan
Keyword(s):  
Storage Time ◽  
Aloe Vera ◽  
Egg Yolk ◽  
Egg White ◽  
Egg Weight ◽  
White Index ◽  
Significant Difference ◽  
Chicken Eggs ◽  
Haugh Unit

THE EFFECT OF IMMERSION IN GEL OF ALOE VERA ON THE QUALITY OF  CHICKEN EGGS DURING STORAGE. The study was conducted to determine the duration effect of immersion of chicken eggs in aloe vera extract (Aloe Vera) on the quality of broiler eggs during storage. The material used in this study was freshly bred eggs at first day at weight of 55 - 60 grams. Aloe vera was obtained from the center of the ornamental plant of Bahu Village. The tools used in the study were including knives, scales, blenders, containers, diameter caliper, egg candlling, petri dishes. The method used was a completely randomized design (CRD) method with factorial pattern of a 5 x 5 with 3 replications. Factor A was the immersion time and factor B was the storage time. The observed variables were egg weight, egg white index, egg yolk index, and haugh unit. The data obtained were analyzed using ANOVA. The results of this study indicated that there was no significant difference (P> 0.05) on Egg Weight, however, there were significant differences (P< 0.05) on the egg white Index, Egg Yolk Index and Haugh Unit. Soaking eggs in aloe vera gel could extend the storage time up to 30 days.Keywords: Eggs, Aloe vera, Storage, Physical Quality

scholarly journals Ex-situ exploration of cemani chicken in Balai Penelitian Ternak (Balitnak), Bogor-West Java

2021 ◽  
Vol 33 ◽  
pp. 01001
Author(s):  
Tatan Kostaman ◽  
Soni Sopiyana ◽  
Isbandi ◽  
Tiurma Pasaribu
Keyword(s):  
Egg Quality ◽  
Ex Situ ◽  
Egg Weight ◽  
Commercial Feed ◽  
Native Chicken ◽  
Ad Libitum ◽  
Chicken Eggs ◽  
Average Body ◽  
Average Body Weight

Cemani chicken is the rarest chicken in the world and generally in Indonesia is associated with medication and ritual ceremonies. The purpose of the research to determine the performance of cemani chickens that are kept outside their habitat. A total of 20 hens kept in litter cages was observed in the exploration. The hens were given commercial feed and drinking water was given ad libitum. The observed variable were hen day production (%), first egg weight (g), egg quality, and growth (g). All data obtained were analyzed descriptively. The results showed that hen day production (%) during 6 months of production increased, from 14.96% at the beginning of production to 48.97%. The first egg weight was 31.31 ± 1.43 g with the egg quality is almost the same as the quality of other local/native chicken eggs. The average body weight of DOC was 25.13 g/head, 8 weeks 839.94 g/head (♂) and 759.98 g/head (♀), and 20 weeks 1537.29 g/head (♂) and 1455.18 g/head (♀). The observed from the data obtained, the performance of cemani chicken in Balitnak showed hen day production, first egg weight, and DOC weight was slightly lower, but for 20-weeks bodyweight, growth was relatively higher.

scholarly journals Vegetable pigments in sorghum-based diets for laying hens

2020 ◽  
Vol 12 ◽  
Author(s):  
Donaldo Antônio Nunes Junior ◽  
Heder José D’Avila Lima ◽  
Jean Kaique Valentim ◽  
Laura Aline Zanelatto Souza ◽  
Nayara Emanoelle Matos e Silva ◽  
...  
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Energy Sources ◽  
Natural Pigment ◽  
Randomized Design ◽  
Significant Difference ◽  
Egg Yolks ◽  
Completely Randomized Design

The main objective of this research is to evaluate the inclusion of different natural pigment solutions in diets formulated with maize and sorghum solutions on the growth performance and egg quality of commercial laying hens created in hot climates. Throughout the conduction of this experiment, were used 252 laying hens of the Hisex Brow, in a completely randomized design, with six treatments, seven repetitions, and six birds per plot. The experimental diets were assigned with different energy sources and adding pigmentation solutions, diet 1 (corn), diet 2 (corn + annatto), diet 3 (corn + carrot), diet 4 (sorghum), diet 5 (sorghum + annatto), diet 6 (sorghum + Carrot).  Afterward, being evaluated the performance and quality of eggs. It was observed significant difference with reference t egg production, egg weight (g), gem weight (g), the weight of shell (g), albumin in weight (g), and gem coloration (tons) among the treatments. There was no difference in the parameters of performance and quality of eggs evaluated, only the variable yolk color had a significant effect between treatments. Replacing the energy source of the corn diet with sorghum in the diet of laying hens at peak production provides similar performance and egg quality, which can be a substitute for quality and efficiency. The use of annatto and carrots as a natural pigmentation promotes greater pigmentation of the egg yolks of laying Hisex Brown eggs and does not impair the performance of the birds.

scholarly journals REPRODUCTIVE PERFORMANCE OF DOMESTICATED BROODSTOCK OF SILVER PERCH, Bidyanus bidyanus (MITCHELL 1838) AND THE RELATIONSHIP BETWEEN OIL GLOBULE FRAGMENTATION AND EGG QUALITY

2017 ◽  
Vol 12 (2) ◽  
pp. 43 ◽  
Author(s):  
Sulaeman Sulaeman ◽  
Ravi Fotedar
Keyword(s):  
Survival Rate ◽  
Reproductive Performance ◽  
Egg Quality ◽  
Fertilization Rate ◽  
Hatching Rate ◽  
High Fecundity ◽  
Fertilisation Rate ◽  
Significant Difference ◽  
The Relationship

The experiments investigated the reproductive performance of the domesticated broodstock of the silver perch and the relationship between various degrees of oil globule fragmentation and egg quality. Six years old of second generation broodstock (n=3) were evaluated based on the fecundity, fertilisation rate, hatching rate, the degree of oil fragmentation of egg, and the quality of embryos and larvae produced. The fragmentation were grouped into three categories: un-fragmented (cat-1), moderately fragmented (cat-2), and highly fragmented (cat-3). The results showed that the broodstock had a relatively high fecundity (132,400 ± 7,22), fertilization rate (94.27 ± 1.28%), and hatching rates (87.94 ± 1.23%). The survival rate of larvae at 12 days post hatching (dph) in cat-1 (71.3 ± 0.9%) was higher than cat-2 (66.7 ± 0.9%) whereas cat-2 was higher than cat-3 (61.3 ± 0.3%). The eggs was dominated by cat-1 (78.11 ± 2.44%) which was significantly higher than cat-2 (21.26 ± 2.45%) and cat-3 ones (0.40 ± 0.21%). The survival rate of embryo at 20 hours post spawning (hps) and hatching rate of cat-1 (95.33 ± 0.00% and 93.33 ± 0.00%) and cat-2 (90.00 ± 0.00% and 85.00 ± 0.00%) were significantly higher than cat-3 (72.33 ± 1.76% and 60.33 ± 0.00%). The total length (TL) of the larvae of cat-1 and cat-2 (8.44 ± 0.21 mm and 8.35 ± 0.23 mm respectively) were significantly higher than larvae of cat-3 (7.09 ± 0.14 mm). No significant difference was found in the larval deformities among any categories. In conclusion, the reproductive performance of six year-old broodstock silver perch showing acceptable performance and egg categorisation based on oil globule fragmentation can be used as a useful tool to indicate eggs quality of silver perch.

scholarly journals Physical quality of eggs of four strains of poultry

2021 ◽  
Vol 43 ◽  
pp. e52738
Author(s):  
Guilherme Resende de Almeida ◽  
Michele de Oliveira Mendonça ◽  
Lidianne Carolina de Castro Weitzel ◽  
Tatiana Marques Bittencourt ◽  
Anderson Soares de Matos ◽  
...  
Keyword(s):  
Shell Thickness ◽  
Egg Quality ◽  
Specific Weight ◽  
Shell Weight ◽  
Physical Quality ◽  
Significant Difference ◽  
Great Relevance ◽  
Egg Diameter ◽  
Important Activity

The layer poultry is an important activity for food production with high biological value. Measuring egg quality has great relevance to ensure safety and quality products for consumers. Thus, the objective was to evaluate the egg physical quality of four laying hen’s strains. Were used 864 eggs from four laying hens’ lines (Hisex Brown®, Hy-Line Brown®, Isa Label®, and Lohmann Brown®). The experimental design was completely randomized composed of four treatments (strains) with nine replications with four eggs each. Egg weight, egg diameter, egg length, specific gravity, yolk, albumen and shell weight and percentage, Haugh units, and shell thickness were evaluated. There was a significant difference for all parameters evaluated. The Hisex Brown® strain showed the best results for egg diameter, egg length, specific weight, albumen height, Haugh units, yolk weight, albumen%, shell weight, shell%, and shell thickness, while the Hy-Line Brown® produced bigger and heavier eggs, and Isa Label® presented the highest yolk%. The Hisex Brown® strain showed the better physical quality of eggs when compared to the other studied lines, the strain being indicated when the objective is to produce eggs with better internal and external quality.

scholarly journals Effect of Probiotics on production and egg quality of dual purpose chicken at Kathmundu in Nepal

2019 ◽  
Vol 48 (1) ◽  
pp. 29-35
Author(s):  
D Neupane ◽  
DB Nepali ◽  
N Devkota ◽  
MP Sharma ◽  
IP Kadaria
Keyword(s):  
Weight Gain ◽  
Feed Intake ◽  
Egg Production ◽  
Egg Quality ◽  
Proximate Analysis ◽  
Control Group ◽  
Feed Conversion ◽  
Nutrient Analysis ◽  
Significant Difference

A total of 448, Sakini (224) and Giriraja (224) breed of chickens were used to investigate the feed intake & weight gain from 0-150 days and egg production and egg quality from 150 – 240 days of age in Khumaltar Lalitpur Nepal. The four different levels of probiotics viz. 0,5, 10 and 15 ml of Probiotic (Poultry biosa)/kg of feed were used with randomized factor design 2*4*8(RBD) with 8 treatments and 8 replications. The data was recorded daily on feed intake and egg production and in every 15 days interval for weight gain. Results showed that the addition of probiotic to the feed significantly improved feed intake, weight gain , egg production and egg quality on sakini and giriraja breeds of chickens(P<0.05). Additionally, data on egg production and egg quality showed increased production in treatment group than control group (P<0.05).The general trend revealed increasing level of egg production from five to eight months with significant difference (P<0.05). Nutrient analysis of eggs by proximate analysis in terms of CP, CF, fat and energy showed significant difference (P<0.05). Thus to conclude, there is positive effect of probiotics for production and productivity in terms of feed conversion ratio, weight gain, egg production & egg quality in Sakini and Giriraja breed of Chicken. Bang. J. Anim. Sci. 2019. 48 (1): 29-35

scholarly journals Produksi Telur dan Kualitas Telur Ayam IPB D-1 G7 serta Pendugaan Nilai Ripitabilitasny

2020 ◽  
Vol 8 (2) ◽  
pp. 97-101
Author(s):  
R. Habiburahman ◽  
S. Darwati ◽  
C. Sumantri ◽  
Rukmiasih
Keyword(s):  
Egg Production ◽  
Egg Quality ◽  
Maintenance Management ◽  
Quality Data ◽  
Dual Purpose ◽  
Unit Value ◽  
Parent Stock ◽  
Chicken Eggs ◽  
Haugh Unit

IPB-D1 is a chicken cross between male F1 PS (PelungxSentul) with F1 female (Kampung x parent stock cobb). The establishment of chicken IPB-D1 aims to produce commercial meat type local chicken with semi-intensive maintenance management. The aim of this research to observe study assesses the performance of egg production and the quality of IPB D-1 chicken eggs and estimates the value of repeatability of production and haugh units of IPB D-1 chicken eggs. The chicken was observed as many as 30 hens IPB D-1 G7 aged 24-36 weeks and eggs as many as 288 eggs. The production and egg quality data were analyzed descriptively and the repeatability values were analyzed with Anova. Egg weight 40 g, Haugh unit value 87 (Very good), egg production 49.22%. The repeatability value of egg production is 0.2 (Moderate) and the repeatability value of the haugh unit is 0.31 (Medium). IPB D-1 Chicken has the potential to be a good dual-purpose chicken.

scholarly journals Identification of Bacteria on Seblak Food around University of Jember Based on Microbiological Criteria of Processed Food

BIOEDUKASI ◽  
2020 ◽  
pp. 34
Author(s):  
Rizka Maulidya Cahyani ◽  
Joko Waluyo ◽  
Mochammad Iqbal
Keyword(s):  
Morphological Characteristics ◽  
Hot Water ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Gram Staining ◽  
Microbiological Criteria ◽  
Identification Of Bacteria ◽  
Oxidase Test ◽  
Safe Food

The quality of food that is good in bacteriological, chemical and physical must always be maintained in order to avoid diseases or health problems. Healthy and safe food is an important factor to improve the standard of public health. Seblak is a ready-to-eat Indonesian food which until now has never been carried out research about what kinds of bacteria in it. Seblak is a food made from raw crackers which is then deliberately soaked using hot water to have a chewy texture. This study aims to determine what types of bacteria are contained in seblak, through the process of isolation and identification in the macroscopic, microscopic and biochemical way. This study used 5 samples, which was repeated 5 times for each sample. Bacteria were isolated from the sample using spread plate techniques and observed by growing colonies on the plate. Each different colonies was observed microscopically through gram staining and endospore staining. To strengthen the data, biochemical tests were also carried out, biochemical tests that have been done in this study were the oxidase test, catalase test, and indole test. The results of the study showed that the bacteria that were found from the samples are in the genus of Bacillus sp. because they show the morphological characteristics of the colonies that form concentric circles, meanwhile, microscopic observations show morphological characteristics of cells in the form of gram-negative bacilli and have the endospores.

scholarly journals Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms

2018 ◽  
Vol 68 (3) ◽  
pp. 347
Author(s):  
J. BOJKOVSKI ◽  
V. ILIĆ ◽  
A. VASIĆ ◽  
J. MALETIĆ ◽  
S. VAKANJAC ◽  
...  
Keyword(s):  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Pcv2 Infection ◽  
Porcine Circovirus ◽  
Reproductive Parameters ◽  
Biochemical Tests ◽  
Blood Biochemical ◽  
Significant Difference ◽  
Commercial Farms

The aim of the present study was to determine the porcine circovirus type 2 (PCV2) prevalence in boars in 3 farms in Serbia and their possible relation with alterations of reproductive parameters and blood biochemical parameters [total protein, urea, creatinine and aspartate transaminase levels (AST)]. The prevalence of (PCV2) was evaluated by the presence of specific antibodies. An ELISA assay was used for the detection of PCV2 antibodies in 58 boars’ blood sera from Serbian commercial farms, among which 48 were from and reared in the Serbian farms, and 10 were imported from different European Union (EU) countries. Anti PCV2 IgM and/or IgG were detected in sera of 51 (87.93%) boars. Based on the type of antibodies (IgM and IgG PCV2 antibodies), it was concluded that chronic PCV2 infection was the predominant type in tested boars. The imported boars did not have an active infection titer. Biochemical tests in blood didn’t show significant differences between PCV2 positive and negative boars. The recorded high prevalence of PCV2 antibodies among boars indirectly proved that PCV2 has been wide spread in the examined farms. Significant difference in the frequency of active, recent and chronic PCV2 infection in boars was found among three farms (p<0.05). Imported boars did not show significant difference in terms of active, recent or chronic PCV2 infection in comparison with boars deriving from Serbian farms (p>0.05). Among boars of three different breeds (Landrace, Duroc and Yorkshire), a significantly higher prevalence of PCV2 antibodies was revealed in Landrace boars (p=0.003). Reproductive parameters of sows that were inseminated with PCV2 positive or negative boars’ semen, showed similar farrowing rate, as well as percentage of live and stillborn piglets, thus indicating that the observed chronic PCV2 infection did not affect significantly the breeding capability of boars.

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