scholarly journals Study on Biofilm Formation Process Based on Image Structure Analyzer Software Analysis

2021 ◽  
Vol 83 ◽  
Author(s):  
Z.C. LI
Keyword(s):  
Formation Process ◽  
Biofilm Formation ◽  
Image Structure ◽  
Software Analysis ◽  
Structure Analyzer

Process Description of an Unconventional Biofilm Formation by Bacterial Cells Autoagglutinating Through Sticky, Long, and Peritrichate Nanofibers

2019 ◽  
Author(s):  
Yoshihide Furuichi ◽  
Shogo Yoshimoto ◽  
Tomohiro Inaba ◽  
Nobuhiko Nomura ◽  
Katsutoshi Hori
Keyword(s):  
Formation Process ◽  
Biofilm Formation ◽  
Extracellular Polymeric Substances ◽  
Waste Treatment ◽  
Large Cell ◽  
Dlvo Theory ◽  
Bacterial Cells ◽  
Chemical Production ◽  
Discontinuous Surface ◽  
Cell Cell

<p></p><p>Biofilms are used in environmental biotechnologies including waste treatment and environmentally friendly chemical production. Understanding the mechanisms of biofilm formation is essential to control microbial behavior and improve environmental biotechnologies. <i>Acinetobacter </i>sp. Tol 5 autoagglutinate through the interaction of the long, peritrichate nanofiber protein AtaA, a trimeric autotransporter adhesin. Using AtaA, without cell growth or the production of extracellular polymeric substances, Tol 5 cells quickly form an unconventional biofilm. In this study, we investigated the formation process of this unconventional biofilm, which started with cell–cell interactions, proceeded to cell clumping, and led to the formation of large cell aggregates. The cell–cell interaction was described by DLVO theory based on a new concept, which considers two independent interactions between two cell bodies and between two AtaA fiber tips forming a virtual discontinuous surface. If cell bodies cannot collide owing to an energy barrier at low ionic strengths but approach within the interactive distance of AtaA fibers, cells can agglutinate through their contact. Cell clumping proceeds following the cluster–cluster aggregation model, and an unconventional biofilm containing void spaces and a fractal nature develops. Understanding its formation process would extend the utilization of various types of biofilms, enhancing environmental biotechnologies.</p><p></p>

scholarly journals Effects of Operational Parameters on Biofilm Formation of Mixed Bacteria for Hydrogen Fermentation

2020 ◽  
Vol 12 (21) ◽  
pp. 8863
Author(s):  
Jie Mei ◽  
Huize Chen ◽  
Qiang Liao ◽  
Abdul-Sattar Nizami ◽  
Ao Xia ◽  
...  
Keyword(s):  
Formation Process ◽  
Biofilm Formation ◽  
Laser Scanning ◽  
Extracellular Polymeric Substances ◽  
Packed Bed ◽  
Dark Fermentation ◽  
Laser Scanning Microscopy ◽  
Packed Bed Reactor ◽  
Confocal Laser ◽  
Operational Parameters

Dark fermentation of organic wastes, such as food waste and algae, via mixed hydrogen-producing bacteria (HPB) is considered a sustainable approach for hydrogen production. The biofilm system protects microorganisms from the harmful environment and avoids the excessive loss of bacteria caused by washout, which ensures that the dark fermentation process remains stable. In this study, a downflow anaerobic packed-bed reactor was commissioned to investigate the biofilm formation process of mixed HPB under various operational parameters. Scanning electron microscopy indicated changes in surface morphology during the biofilm formation period. Proteins and polysaccharides in extracellular polymeric substances were identified by confocal laser scanning microscopy to reveal their distribution characteristics. A hydraulic retention time of 0.5 d, a substrate concentration of 15 g/L and an HPB inoculum ratio of 35% were identified as the optimal operational parameters for biofilm formation. The diversity of bacteria between suspension and biofilm showed significantly different distributions; Clostridiales and Lactobacillales were identified as the dominant orders in the biofilm formation process. The abundances of Clostridiales and Lactobacillales were 15.1% and 56.2% in the biofilm, respectively.

Analysis on the Start-up of Campus Sewage Treatment with 3-Stage Biological Aerated Filter

2013 ◽  
Vol 295-298 ◽  
pp. 1376-1379
Author(s):  
Lei Zhu ◽  
Fang Xing Liu ◽  
Xiao Lin Jiang ◽  
Hong Jiao Song
Keyword(s):  
Removal Efficiency ◽  
Formation Process ◽  
Biofilm Formation ◽  
Sewage Treatment ◽  
Biological Aerated Filter ◽  
Filter System ◽  
N Concentration ◽  
Start Up ◽  
Formation Method ◽  
Aerated Filter

In this study, the alternating 3-stage biological aerated filter system with the brush as the filler was proposed for campus sewage treatment and the biofilm formation process was researched. Adopting the four-stage inoculated biofilm formation method, the treatment effects of the 3 filter columns respectively lasted 23d, 20d, 23d to reach stable. After the attached biofilm grew steadily, the effluent COD concentration was between 45 mg/L and 95 mg/L, and the removal efficiency was 77%~85%. The effluent NH4+-N concentration of 1st and 3rd filter columns was 11~25 mg/L, and the removal efficiency was 47%~67%; while the effluent NH4+-N concentration of 2nd filter column was 8 ~19 mg/L, the removal efficiency was 64%~ 78%.

In situ monitoring of wastewater biofilm formation process via ultrasonic time domain reflectometry (UTDR)

2018 ◽  
Vol 334 ◽  
pp. 2134-2141 ◽  
Author(s):  
Jinfeng Wang ◽  
Hongqiang Ren ◽  
Xianhui Li ◽  
Jianxin Li ◽  
Lili Ding ◽  
...  
Keyword(s):  
Time Domain ◽  
Formation Process ◽  
Biofilm Formation ◽  
Time Domain Reflectometry ◽  
In Situ Monitoring ◽  
Ultrasonic Time

Influence of Small RNAs on Biofilm Formation Process in Bacteria

2013 ◽  
Vol 55 (3) ◽  
pp. 288-297 ◽  
Author(s):  
Mohammad Ali Ghaz-Jahanian ◽  
Fatemeh Khodaparastan ◽  
Aydin Berenjian ◽  
Hoda Jafarizadeh-Malmiri
Keyword(s):  
Formation Process ◽  
Biofilm Formation ◽  
Small Rnas

Shotgun proteomics study of early biofilm formation process of Acidithiobacillus ferrooxidans ATCC 23270 on pyrite

PROTEOMICS ◽  
2013 ◽  
Vol 13 (7) ◽  
pp. 1133-1144 ◽  
Author(s):  
Mario Vera ◽  
Beate Krok ◽  
Sören Bellenberg ◽  
Wolfgang Sand ◽  
Ansgar Poetsch
Keyword(s):  
Formation Process ◽  
Acidithiobacillus Ferrooxidans ◽  
Biofilm Formation ◽  
Shotgun Proteomics ◽  
Proteomics Study

scholarly journals Assessing toxicity of titanium dioxide (TiO2) nanoparticles on Pseudomonas species biofilms

2021 ◽  
Author(s):  
Yevheniya Chabanyuk
Keyword(s):  
Titanium Dioxide ◽  
Biofilm Formation ◽  
Laser Scanning ◽  
Titanium Dioxide Nanoparticles ◽  
Viable Cell ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Long Term Effects ◽  
Software Analysis ◽  
Cell Counts

Biofilms are essential to the aquatic environment. Recent advances in technology resulted in increased use of nanomaterials (such as titanium dioxide nanoparticles) and their release into aquatic environments with unknown long-term effects. Potential toxicity of titanium dioxide, known for its photocatalytic properties, on Pseudomonas aeruginosa (PAO1-gfp) and Pseudomonas sp. (CT07-gfp) biofilm formation and proliferation was assessed using flowcells, confocal laser scanning microscopy (CLSM), and total and viable cell release into effluent under different titanium dioxide concentrations (100 ppm, 10 ppm and 1 ppm). COMSTAT software analysis was used to obtain quantitative morphological biofilm data. Results showed that titanium dioxide had a concentration and media-dependent effect on biofilm formation, growth, proliferation and viability. Viable effluent cell counts remained within the same order of magnitude. Biofilm recovery was evident within 24-48 hours after exposure. At environmentally relevant concentration (1 ppm), there was no effect on formation, proliferation or growth of the biofilm.

scholarly journals Influence of antibiotics on biofilm formation by Streptococcus pyogenes in vitro

2021 ◽  
Vol 98 (1) ◽  
pp. 59-64
Author(s):  
A. A. Adzhieva ◽  
T. A. Danilova ◽  
G. A. Danilina ◽  
N. V. Shevlyagina ◽  
A. G. Minko ◽  
...  
Keyword(s):  
Streptococcus Pyogenes ◽  
Formation Process ◽  
Biofilm Formation ◽  
Water Solution ◽  
Spherical Shape ◽  
Inhibitory Effect ◽  
Destructive Effect ◽  
Alcohol Water ◽  
Negative Effect

Aim. To study the effect of the antibiotics oxacillin and cefazolin on culture growth and biofilm formation of Streptococcus pyogenes type 30M in vitro.Materials and methods. The formation of biofilms was studied in light and scanning electron microscope, on sterile cover glasses using special nutrient medium. To identify the biofilm, experimental samples were stained with alcohol-water solution of alcian blue.Results. Small quantitative doses (0.01–0.1 mg) of oxacillin and cefazoline, regardless of the duration of the exposure, did not have a negative effect both on the biofilm formation process and the formed streptococcal biofilm. An increase in quantitative doses of antibiotics to 0.5–1.0 mg at the stage of formation of Streptococcus biofilm had an inhibitory effect on the growth of microbial cells and the formation of biofilm. The integrity of streptococcal chains and biofilm was preserved when oxacillin and cefazoline were applied at a quantity of 0.5–1.0 mg to the formed biofilm. However, in some cases, the twisting of the chains in spherical shape with visible colored biofilm inside was observed. In addition, there was a stimulating effect of cefazoline on the formed Streptococcal biofilm. When the formed biofilm was exposed to high doses of antibiotics (2 mg or higher), a break of streptococcal chains was observed, while oxacillin had a destructive effect on the microbial biofilm. In similar experiments with cefazolin, the biofilm was preserved, despite the fact that the destruction of microbial chains was also observed.Conclusion. Significant differences were found in the effect of tested antibiotics on both the mature S. pyogenes type 30M biofilm and the biofilm formation process.

Sign in / Sign up

Export Citation Format

Share Document